Diagnosis on improved electrocatalytic activity of La₂Ni_0.8Cu_0.2O_4+δ electrodes towards oxygen reduction reaction

Wang, Ya-Ping,Xu, Qing,Huang, Duan-Ping,Zhao, Kai,Chen, Min,Kim, Bok-Hee Elsevier 2017 APPLIED SURFACE SCIENCE - Vol.423 No.-

<P><B>Abstract</B></P> <P>La<SUB>2</SUB>Ni<SUB>0.8</SUB>Cu<SUB>0.2</SUB>O<SUB>4+δ</SUB> electrodes with different thicknesses were prepared on Ce<SUB>0.8</SUB>Sm<SUB>0.2</SUB>O<SUB>1.9</SUB> electrolyte using screen printing technique. The electrocatalytic activity of La<SUB>2</SUB>Ni<SUB>0.8</SUB>Cu<SUB>0.2</SUB>O<SUB>4+δ</SUB> electrodes towards oxygen reduction reaction was investigated in comparison with La<SUB>2</SUB>NiO<SUB>4+δ</SUB> electrode. To understand the electrocatalytic activity of the two electrodes, chemical states of constituent elements on the surface of La<SUB>2</SUB>NiO<SUB>4+δ</SUB> and La<SUB>2</SUB>Ni<SUB>0.8</SUB>Cu<SUB>0.2</SUB>O<SUB>4+δ</SUB> were analyzed using X-ray photoelectron spectroscopy technique. La<SUB>2</SUB>Ni<SUB>0.8</SUB>Cu<SUB>0.2</SUB>O<SUB>4+δ</SUB> electrodes sintered at 920°C exhibited improved electrocatalytic activity relative to literature results of the electrodes sintered at higher temperatures (e.g. 1200°C). The Cu doping in La<SUB>2</SUB>NiO<SUB>4+δ</SUB> was validated to be effective in enhancing electrocatalytic activity. The electrocatalytic activity of La<SUB>2</SUB>Ni<SUB>0.8</SUB>Cu<SUB>0.2</SUB>O<SUB>4+δ</SUB> electrodes was found to be dependent on their thickness. Optimal electrocatalytic activity was achieved for the electrode with a thickness of 10μm, showing a polarization resistance of 0.13Ω cm<SUP>2</SUP> at 800°C. The effects of Cu doping and electrode thickness were diagnosed with respect to contributions of involved electrode reaction steps. The results of this work underscore that the electrocatalytic activity of La<SUB>2</SUB>Ni<SUB>0.8</SUB>Cu<SUB>0.2</SUB>O<SUB>4+δ</SUB> electrodes is dictated by dissociative adsorption process of molecular oxygen on the surface.</P> <P><B>Highlights</B></P> <P> <UL> <LI> LNC electrodes achieved improved electrocatalytic activity than literature results. </LI> <LI> The electrocatalytic activity of LNC electrodes is higher than LN electrode. </LI> <LI> The electrocatalytic activity of LNC electrodes is dependent on thickness. </LI> <LI> The optimal electrode polarization resistance of LNC is 0.13Ω cm<SUP>2</SUP> at 800°C. </LI> <LI> The effects of Cu doping and electrode thickness have been explained. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Survey on electrochemical properties of La_2−xSr_xNiO_4±δ (x = 0.2 and 0.8, δ > 0) cathodes related with structural stability under cathodic polarization conditions

Wang, Ya-Ping,Xu, Qing,Huang, Duan-Ping,Zhao, Kai,Chen, Min,Kim, Bok-Hee Elsevier 2017 International journal of hydrogen energy Vol.42 No.9

<P><B>Abstract</B></P> <P>In view of utilization as cathodes for intermediate temperature solid oxide fuel cells (SOFCs), the La<SUB>2−x</SUB>Sr<SUB>x</SUB>NiO<SUB>4±δ</SUB> (x = 0.2 and 0.8, δ > 0) series of compounds were evaluated with respect to their electrical conducting, thermomechanical and electrochemical properties. On the one hand, La<SUB>1.2</SUB>Sr<SUB>0.8</SUB>NiO<SUB>4−δ</SUB> exhibits a higher electrical conductivity (>180 S cm<SUP>−1</SUP> in the temperature range 600–800 °C) and a lower electrode polarization under open-circuit voltage conditions (∼0.12 Ω cm<SUP>2</SUP> at 800 °C) than La<SUB>1.8</SUB>Sr<SUB>0.2</SUB>NiO<SUB>4+δ</SUB>, together with a suitable thermal expansion coefficient (13.8 × 10<SUP>−6</SUP> K<SUP>−1</SUP> averaged between 50 and 1000 °C). On the other hand, the structure and electrochemical properties of La<SUB>1.2</SUB>Sr<SUB>0.8</SUB>NiO<SUB>4−δ</SUB> electrode were obviously changed after being subjected to cathodic polarization. The cathodically polarized La<SUB>1.2</SUB>Sr<SUB>0.8</SUB>NiO<SUB>4−δ</SUB> electrode showed an aggregation of strontium oxide onto the surface, concomitant with a degradation of electrochemical properties. By contrast, the structure and electrochemical properties of La<SUB>1.8</SUB>Sr<SUB>0.2</SUB>NiO<SUB>4+δ</SUB> electrode before and after cathodic polarization were basically unvaried. The disparate changes in structure and electrochemical properties of the two electrodes were interpreted in terms of their differing peculiarities in oxygen nonstoichiometry. The results of this work highlight the importance of strontium content and thereby oxygen nonstoichiometry of La<SUB>1−x</SUB>Sr<SUB>x</SUB>NiO<SUB>4±δ</SUB> electrodes to their structural stability under cathodic polarization conditions, which is a critical point to be used as cathodes in SOFCs.</P> <P><B>Highlights</B></P> <P> <UL> <LI> LSN2 and LSN8 were evaluated in view of cathode utilization for IT-SOFCs. </LI> <LI> LSN8 exhibits higher electrical conductivity and catalytic activity than LSN2. </LI> <LI> Cathodically polarized LSN8 electrode undergoes structure and property changes. </LI> <LI> The structure and properties of polarized LSN2 electrode remains unchanged. </LI> <LI> The disparate behaviors of the two electrodes were interpreted. </LI> </UL> </P>

原解釋 - 中國文化的符號化動態結構

황아평 ( Ya Ping Huang ) 中國語文硏究會 2005 中國語文論叢 Vol.0 No.29

Ni/La2O3-ZrO2 catalyst for hydrogen production from steam reforming of acetic acid as a model compound of bio-oil

Ya-ping Xue,Chang-feng Yan,Xiao-yong Zhao,Shi-lin Huang,Chang-qing Guo 한국화학공학회 2017 Korean Journal of Chemical Engineering Vol.34 No.2

Hydrogen production from steam reforming of acetic acid was investigated over Ni/La2O3-ZrO2 catalyst. A series of Ni/La2O3-ZrO2 catalysts were synthesized by sol-gel method coupled with wet impregnation, which was characterized by XRD, BET, TEM, EDS, TG, SEM and TPR. Catalytic activity of Ni/La2O3-ZrO2 was evaluated by steam reforming of acetic acid at the temperature range of 550-750 oC. The tetragonal phase La0.1Zr0.9O1.95 is formed through the doping of La2O3 into the ZrO2 lattice and nickel species are highly dispersed on the support with high specific surface area. H2 yield and CO2 yield of Ni/La2O3-ZrO2 catalyst with 15%wt Ni reaches 89.27% and 80.41% at 600 oC, respectively, which is attributed to high BET surface area and sufficient Ni active sites in strong interaction with the support. 15%wt Ni supported on La2O3-ZrO2 catalyst maintains relatively stable catalytic activities for a period of 20 h.

Inhibition of c-FLIP by RNAi Enhances Sensitivity of the Human Osteogenic Sarcoma Cell Line U2OS to TRAILInduced Apoptosis

Zhang, Ya-Ping,Kong, Qing-Hong,Huang, Ying,Wang, Guan-Lin,Chang, Kwen-Jen Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.6

To study effects of cellular FLICE (FADD-like IL-$1{\beta}$-converting enzyme)-inhibitory protein (c-FLIP) inhibition by RNA interference (RNAi) on sensitivity of U2OS cells to tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL)-induced apoptosis, plasmid pSUPER-c-FLIP-siRNA was constructed and then transfected into U2OS cells. A stable transfection cell clone U2OS/pSUPER-c-FLIP-siRNA was screened from the c-FLIP-siRNA transfected cells. RT-PCR and Western blotting were applied to measure the expression of c-FLIP at the levels of mRNA and protein. The results indicated that the expression of c-FLIP was significantly suppressed by the c-FLIP-siRNA in the cloned U2OS/pSUPER-c-FLIP-siRNA as compared with the control cells of U2OS/pSUPER. The cloned cell line of U2OS/pSUPER-c-FLIP-siRNA was further examined for TRAILinduced cell death and apoptosis in the presence of a pan-antagonist of inhibitor of apoptosis proteins (IAPs) AT406, with or without 4 hrs pretreatment with rocaglamide, an inhibitor of c-FLIP biosynthesis, for 24 hrs. Cell death effects and apoptosis were measured by the methods of MTT assay with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and flow cytometry, respectively. The results indicated that TRAIL-induced cell death in U2OS/pSUPER-c-FLIP-siRNA was increased compared with control cells U2OS/pSUPER in the presence or absence of AT406. Flow cytometry indicated that TRAIL-induced cell death effects proceeded through cell apoptosis pathway. However, in the presence of rocaglamide, cell death or apoptotic effects of TRAIL were similar and profound in both cell lines, suggesting that the mechanism of action for both c-FLIP-siRNA and rocaglamide was identical. We conclude that the inhibition of c-FLIP by either c-FLIP-siRNA or rocaglamide can enhance the sensitivity of U2OS to TRAIL-induced apopotosis, suggesting that inhibition of c-FLIP is a good target for anti-cancer therapy.

Identification of the DNA Binding Element of the Human ZNF333 Protein

( Zhe Jing ),( Ya Ping Liu ),( Min Dong ),( Shao Yi Hu ),( Shang Zhi Huang ) 생화학분자생물학회 2004 BMB Reports Vol.37 No.6

ZNF 333 is a new and sole gene containing two KRAB domains which has been identified currently. It is a member of subfamilies of zinc finger gene complex which had been localized on chromosome 19p13.1. The ZNF333 gene mainly encodes a 75.5 kDa protein which contains 10 zinc finger domains. Using the methods of random oligonucleotide selection assay, electromobility gel shift assay and luciferase activity assay, we found that ZNF333 recognized the specific DNA core binding sequence ATAAT. Moreover, these data indicated that the KRAB domain of ZNF333 really has the ability of transcriptional repression.

BMB reports : Characterization of a novel Cotesia vestalis polydnavirus (CvBV) gene containing a ser-rich motif expressed in Plutella xylostella Larvae

( Min Shi ),( Ya Feng Chen ),( Fang Huang ),( Xue Ping Zhou ),( Xue Xin Chen ) 생화학분자생물학회 2008 BMB Reports Vol.41 No.8

Cotesia vestalis is an endoparasitoid of Plutella xylostella larvae and injects a polydnavirus (CvBV) into its host during oviposition. In this report we characterize the gene, CvBV3307, and its products. CvBV3307 is located on segment S33 of the CvBV genome, is 517 bp, and encodes a putative protein of 122 amino acids, including a serine-rich region. The expression pattern of CvBV3307 in parasitized larvae and the subcellular localization of CvBV3307 only in granulocytes indicated that it might be involved in early protection of parasitoid eggs from host cellular encapsulation and in manipulating the hormone titer and developmental rhythm of host larvae. Western blot analysis showed that the size of the immunoreactive protein (about 55 kDa) in parasitized hosts at 48 hours post parasitization (h p.p.) is much larger than the predicted molecular weight of 13.6 kDa, which suggests that CvBV3307 undergoes extensive post-translational modification in hosts.

ALGORITHMS FOR SYSTEMS OFNONLINEAR VARIATIONAL INEQUALITIES

Yeol Je Cho,Ya-ping Fang,Nan-jing Huang,Ho Jin Hwang 대한수학회 2004 대한수학회지 Vol.41 No.3

In this paper, we introduce and study a new system of nonlinear variational inequalities. The existence and uniqueness of solution for this problem are proved and an iterative algorithm for approximating the solution of system of nonlinear variational inequalities is constructed.

Chlorophyll-Related Compounds Inhibit Cell Adhesion and Inflammation in Human Aortic Cells

Kuan-Hung Lin,Ching-Yun Hsu,Ya-Ping Huang,Jun-You Lai,Wen-Bin Hsieh,Meng-Yuan Huang,Chi-Ming Yang,Pi-Yu Chao 한국식품영양과학회 2013 Journal of medicinal food Vol.16 No.10

The objectives of this study were to investigate the effects of chlorophyll-related compounds (CRCs) and chlorophyll (Chl) a + b on inflammation in human aortic endothelial cells. Adhesion molecule expression and interleukin (IL)-8, nuclear factor (NF)-jB p65 protein, and NF-jB and activator protein (AP)-1 DNA binding were assessed. The effects of CRCs on inflammatory signaling pathways of signal transducers and activators of transcription 3 (STAT3) and mothers against decapentaplegic homolog 4, respectively induced by IL-6 and transforming growth factor (TGF)-b, in human aortic smooth muscle cells cultured in vitro were also investigated. HAECs were pretreated with 10 lM of CRCs, Chl a + b, and aspirin (Asp) for 18 h followed by tumor necrosis factor (TNF)-a (2 ng/mL) for 6 h, and U937 cell adhesion was determined. TNF-a–induced monocyte-endothelial cell adhesion was significantly inhibited by CRCs. Moreover, CRCs and Chl a + b significantly attenuated vascular cell adhesion molecule-1, intercellular adhesion molecule-1, and IL-8 expressions. Treatments also significantly decreased in NF-jB expression, DNA binding, and AP-1 DNA binding by CRCs and Asp. Thus, CRCs exert anti-inflammatory effects through modulation of NF-jB and AP-1 signaling. Ten micromoles of CRCs and Asp upregulated the expression of mothers against decapentaplegic homolog 4 (Drosophila) (SMAD4) in the TGF-b receptor signaling pathway, and SMAD3/4 transcription activity was also increased. Ten micromoles of CRCs were able to potently inhibit STAT3-binding activity by repressing IL-6–induced STAT3 expression. Our results provide a potential mechanism that explains the anti-inflammatory activities of these CRCs.

Experience in Professional Resilience for Nurses Caring for Patients with COVID-19: A Qualitative Descriptive Study

Chiu Pai-En,Lin Shu-Chuan A.,Li Ya-Ping,Huang Chiao-Hsin,Shu Ying-Mei,Chen Chi-Wen 한국간호과학회 2024 Asian Nursing Research Vol.18 No.1

Purpose During the COVID-19 pandemic, nurses have faced many professional and ethical dilemmas and challenges along with bearing physical, mental, and emotional stress resulting from worrying about themselves or their family being infected and stigmatized. This stress can potentially lead to burnout and resignation. Professional resilience is crucial for nurses to cope with these adverse situations. This study aimed to investigate the process by which nurses adapt, change, and overcome challenges during the COVID-19 pandemic and ultimately demonstrate professional resilience. Methods Descriptive phenomenology was applied. Semi-structured interviews were conducted with 11 nurses working in COVID-19 wards and intensive care units to collect data. Giorgi's phenomenological analysis method was employed. Results Based on the interview responses, four major themes were identified: 1) balancing patient care, self-protection, and passing on experience; 2) providing timely pandemic team resources and social support; 3) nurses' perseverance amid social discourse and constrained lives; and 4) selfless dedication shaping nursing's pinnacle experiences. Conclusions In the face of a sudden pandemic, frontline nurses play a critical role in maintaining medical capacity. Consequently, they must balance their families, lives, and work while adapting to the impact of the pandemic and changing practices and procedures based on the development of the pandemic and policy demands. The study findings provide insights into the challenges and emotional experiences encountered by nurses during a sudden pandemic outbreak and can serve as a reference for developing strategies to help nurses overcome these challenges and enhance their professional resilience.