Cell Surface Nano-modulation for Non-invasive in-vivo Near-IR Stem Cell Monitoring

Shin, W. J.,Shin, S. W.,Yuk, J. S.,Amornkitbamrung, L.,Jang, M. S.,Song, I. H.,Choi, S. W.,Kang, I.,Lee, J. Y.,Bae, H. JOHN WILEY SONS LTD 2017 ChemMedChem Vol.12 No.1

<P>A stem cell tracking system is in high demand for the determination of cell destinations and for the validation of cell therapeutic efficacy in regenerative transplantation. To date, near-infrared (NIR) imaging technology has received considerable attention in cell behavior monitoring, owing to its patient compatibility, easy accessibility and cost effectiveness. Conventionally, invivo cell tracking has been visualized by direct in-cell staining with NIR, where it may be achieved by complicated genetic engineering. Such genetic amendment techniques have suffered from serious challenges, which can destroy a cell's metabolism and can accidentally incur unexpected carcinoma. Herein we demonstrate a novel cell nano-modulation method for noninvasive stem cell monitoring. It is simply achieved by conjugating stem cells with lipid-supported, NIR-tagged, polymeric nanoparticles. These engineered cells, which are designated as NIR-labeled light-emitting stem cells (LESCs), maintain their biochemical functionality (i.e., differentiation, quantum efficacy, etc.) even after conjugation. LESCs were used for insitu stem cell monitoring at inoculation sites. It is speculated that the LESC technique could provide a new preparative methodology for invivo cell tracking in advanced diagnostic medicine, where cell behavior is a critical issue.</P>

BOAO PHOTOMETRIC SURVEY OF GALACTIC OPEN CLUSTERS. I. BERKELEY 14, COLLINDER 74, BIURAKAN 9, and NGC 2355

ANN H. B.,LEE M. G.,CHUN M. Y.,KIM S.-L.,JEON Y.-B.,PARK B.-G.,YUK I.-S.,SUNG H.,LEE S. H. The Korean Astronomical Society 1999 Journal of The Korean Astronomical Society Vol.32 No.1

Open clusters are useful tools to investigate the structure and evolution of the Galactic disk. We have started a long-term project to obtain UBVI CCD photometry of open clusters which were little studied before, using the Doyak 1.8 m telescope of Bohyunsan Optical Astronomy Observatory in Korea. The primary goals of this project are (1) to make a catalog of UBVI photometry of open clusters, (2) to make an atlas of open clusters, and (3) to survey and monitor variable stars in open clusters. Here we describe this project and report the first results based on preliminary analysis of the data on four open clusters in the survey sample: Be 14, Cr 74, Biu 9, and NGC 2355. Isochrone fitting of the color-magnitude diagrams of the clusters shows that all of them are intermediate age to old (0.3-1.6 Gyrs) open clusters with moderate metallicity.

가축분의 사료화를 위한 연구 9 . 수집계절 , 수집방법 및 건조방법이 계분의 영양소함량에 미치는 영향

이택원,육종융,한인규,정정수,유문일,정천용 ( Tack W . Lee,Chong Y . Yuk,In K . Han,Chung S . Chung,Moon I . Yoo,Chun Y . Chung ) 한국축산학회 1978 한국축산학회지 Vol.20 No.5

This experiment was conducted to investigate the changes in chemical composition of dehydrated poultry waste (DPW) as affected by season (spring, summer, autumn, winter), collection intervals (12, 24, 36, 48 hours) and drying process (oven dry, oven dry often solar exposure for I-day, oven dry after solar exposure for 2-day, oven dry after solar exposure for 3-day, oven dry after solar exposure for 4-day). The waste was collected from laying hens of White Leghorn strain at Livestock Experiment Station and College of Agriculture, Seoul National University. Fresh hens excreta was dried in drying even at 80℃ for 24 hours after various preliminary treatment as originally designed. The results obtained are summarized as follows: 1. Crude protein content of dries poultry waste collected in winter (38.40%) way much higher than that of spring (28.63%), autumn (24.91%) and summer (22.20%). However, no definite; trend by season was found in the contents of crude fat, crude fiber, crude ash and nitrogen free extract (NFE). 2. Although the collection intervals had no effect on the contents of crude fat. crude fiber, crude ash and NFE, the crude protein content of DPW collected at 12 hours interval (30.15%) showed the higher than the at 24 hours (29.62%), 36 hours (27.70%), or 48 hours (27.20%). 3. It was found that the crude protein content of DPW dried in drying oven at 80℃ was analyzed to be 34.45% and that of DPW dried in oven after solar exposure for 1 day, 2 days, 3 days and 4 days was found to be 30.19%, 28.32%, 27.75% respectively. Drying process had no effect on the other chemical components of DPW studies. 4. Present data indicated that the content of crude protein in DPW could best be retained when fresh poultry waste was dried in oven at 80℃ regardless of collection intervals. It was also apparent that nitrogen loss was proportional to the length of collection intervals, even if same method of drying process was used. It appeared that the content of crude protein and fat was more easily affected by season, collection interval and dying method than the other chemical components.

면양에 대한 국산옥수수 펠릿트의 성장능력 , 소화율 및 제1위내 VFA조성에 미치는 영향

최윤재,육종융,한인규,류연선,배동호 ( Y . J . Choi,C . Y . Yuk,I . K . Han,Y . S . Ryu,D . H . Bae ) 한국축산학회 1982 한국축산학회지 Vol.24 No.4

This experiment was conducted for a period of 140 days to evaluate feeding values, digestibility and volatile fatty acid (VFA) composition of ruminal juice of whole corn crop pellet and corn ear with husk pellet through feeding trial, digestibility experiment and the determination of ruminal pH and volatile fatty acid pattern for lamb. Twelve male lambs weighing 30㎏ in initial body weight were divided into 4 treatments i.e. control, whole corn crop pellet, corn ear with husk pellet and rice straw pellet group. The results obtained are summarized as follows. 1. The body weight gain of lambs fed whole corn crop pellet (1,254.8g) and corn ear with husk pellet group (1,253.9g) was higher than that of rice straw pellet fed group (1,194.3g) or control group. And control group (974.7g) was the lowest. The body weight gain of whole corn crop pellet group was significantly (p$lt;0.05) heavier than that of control group. While, there were no significant differences among the other treatments. Daily dry matter consumption and feed efficiency showed the same tendency as the body weight gain. 2. Digestibilities of the dry matter, crude protein, crude fiber and nitrogen free extract (NFE) in the corn pellets were lower than those in the commercial formula feed, but were higher or slightly lower than those in hay or rice straw pellet. While, crude fat digestibilities of corn pellets were the highest among treatments. Digestible crude protein contents of the whole com crop pellet and corn ear with husk pellet were found to be 5.86 and 6.59%, respectively. Total digestible nutrients (TDN) values of the whole corn crop pellet (62.74%) and corn ear with husk pellet (69.00%) were lower than that of commercial formula feed (72.26%), but higher than that of hay (59.61%) by 3-9%. 3. The changes in ruminal VFA production in molar % were characterized by 4-5% decrease in acetate production and 3-4% and 2-3% increases in propionate and butyrate production during 20 days after feeding the experimental feed, but were not affected by treatments. Tonal VFA production of corn pellet groups was higher than that of rice straw pellet or control group. The ruminal pH was decreased from 0 day to 10 days after feeding experimental diets, but was not changed thereafter regardless of treatments. According to the results of obtained from the present studies, it could be suggested that the use of whole corn crop pellet and corn ear with husk pellet may be used as a combined feed of roughage and grains for ruminant animals and will also help to contribute the use of self produced feedsruffs.

Doxorubicin/heparin composite nanoparticles for caspase-activated prodrug chemotherapy

Khaliq, N.U.,Sandra, F.C.,Park, D.Y.,Lee, J.Y.,Oh, K.S.,Kim, D.,Byun, Y.,Kim, I.S.,Kwon, I.C.,Kim, S.Y.,Yuk, S.H. IPC Science and Technology Press 2016 Biomaterials Vol.101 No.-

<P>Caspase-activated prodrug chemotherapy is introduced and demonstrated using the composite nano particles (NPs), which deliver doxorubicin (DOX) and DEVD-S-DOX together to the tumor tissue. DEVD-S-DOX, DOX linked to a peptide moiety (DEVD), is a prodrug that is cleaved into free DOX by caspase-3 upon apoptosis. DEVD-S-DOX has no therapeutic efficacy, but it changes into free DOX with the expression of caspase-3. With the accumulation of the composite NPs in the tumor tissue by the enhanced permeation and retention (EPR) effect, a small exposure of DOX in the tumor. cells initiated apoptosis in a localized area of the tumor tissue, which induced caspase-3 activation. Cleavage of DEVD-S-DOX into free DOX by caspase-3 continued with repetitive activation of caspase-3 and cleavage of DEVD-S-DOX at the tumor site. The composite NPs were characterized with transmittance electron microscopy (TEM) and particle size analyzer. We then evaluated the nanoparticle drug release, therapeutic efficacy, and in vivo biodistribution for tumor targeting using a non-invasive live animal imaging technology and the quantification of DOX with high performance liquid chromatography. DOX-induced apoptosis-targeted chemotherapy (DIATC) was verified by in vitro/in vivo DEVD-S-DOX response to free DOX and cellular uptake behavior of the composite NPs with flow cytometry analysis. Significant antitumor efficacy with minimal cardiotoxicity was also observed, which supported DIATC for improved chemotherapy. (C) 2016 Elsevier Ltd. All rights reserved.</P>

과학기술위성 1호 원자외선 분광기 FIMS의 배플 설계

Yuk, I.S.,Seon, K.I.,Ryu, K.S.,Jin, H.,Park, J.H,Nam, U.W.,Lee, D.H.,Oh, S.H.,Rhee, J.G.,Han, W.Y.,Min, K.W.,Edelstein, Jerry,Korpela, Eric 한국천문학회 2003 天文學論叢 Vol.18 No.1

FIMS (Far-ultraviolet IMaging Spectrograph) is the main payload of STSAT-1 satellite which was successfully launched on September 27, 2003. The optical system of FIMS consists of two sets of parabolic cylinder mirror, slit, ellipsoidal reflection grating, and baffle system. We designed two types of baffle system for the FIMS: FOV baffle and order baffle. FOV baffle in the mirror house controls the field of view, and the order baffle in the vacuum box blocks the rays reflected rays by different orders.

On the Mechanical Structure of the FIMS

Yuk, I.S.,Ryu, K.S.,Park, J.H.,Seon, K.I.,Nam, U.W.,Han, W.,Seon, J.,Min, K.W.,Korpela, E.J.,Edelstein, J. 한국우주과학회 2000 한국우주과학회보 Vol.9 No.2

Levonorgestrel-Releasing Intrauterine Systems Versus Oral Cyclic Medroxyprogesterone Acetate in Endometrial Hyperplasia Therapy: A Meta-Analysis

Yuk, J. S.,Song, J. Y.,Lee, J. H.,Park, W. I.,Ahn, H. S.,Kim, H. J. Springer New York LLC 2017 Annals of Surgical Oncology Vol.24 No.5

<P>The LNG-IUS treatment has a higher regression rate than cyclic MPA in non-atypical endometrial hyperplasia and mixed endometrial hyperplasia therapy for non-obese women but has a similar regression rate, albeit limited, for obese women.</P>

Comparison between dot-immunoblotting assay and clinical sign determination method for quantifying avian infectious bronchitis virus vaccine by titration in embryonated eggs

Yuk, S.s.,Kwon, J.H.,Noh, J.Y.,Hong, W.t.,Gwon, G.B.,Jeong, J.H.,Jeong, S.,Youn, H.N.,Heo, Y.H.,Lee, J.B.,Park, S.Y.,Choi, I.S.,Song, C.S. Elsevier/North-Holland Biomedical Press 2016 JOURNAL OF VIROLOGICAL METHODS Vol.230 No.-

A sensitive and specific method for measuring the vaccine titer of infectious bronchitis virus (IBV) is important to commercial manufacturers for improving vaccine quality. Typically, IBV is titrated in embryonated chicken eggs, and the infectivity of the virus dilutions is determined by assessing clinical signs in the embryos as evidence of viral propagation. In this study, we used a dot-immunoblotting assay (DIA) to measure the titers of IBV vaccines that originated from different pathogenic strains or attenuation methods in embryonated eggs, and we compared this assay to the currently used method, clinical sign evaluation. To compare the two methods, we used real-time reverse transcription-PCR, which had the lowest limit of detection for propagated IBV. As a clinical sign of infection, dwarfism of the embryo was quantified using the embryo: egg (EE) index. The DIA showed 9.41% higher sensitivity and 15.5% higher specificity than the clinical sign determination method. The DIA was particularly useful for measuring the titer of IBV vaccine that did not cause apparent stunting but propagated in embryonated chicken eggs such as a heat-adapted vaccine strain. The results of this study indicate that the DIA is a rapid, sensitive, reliable method for determining IBV vaccine titer in embryonated eggs at a relatively low cost.

Efficacy of clade 2.3.2 H5 commercial vaccines in protecting chickens from clade 2.3.4.4 H5N8 highly pathogenic avian influenza infection

Yuk, S.s.,Erdene-Ochir, T.O.,Kwon, J.H.,Noh, J.Y.,Hong, W.t.,Jeong, J.H.,Jeong, S.,Gwon, G.B.,Shin, J.i.,Sur, J.H.,Song, C.S. Butterworths ; Elsevier Science Ltd 2017 Vaccine Vol.35 No.9

Emerging clade 2.3.4.4 of the highly pathogenic avian influenza (HPAI) virus strain H5N8, which had been detected sporadically in domestic poultry in China, started to affect wild birds and poultry in South Korea in 2014. The virus was spread to Germany, Italy, the Netherlands, United Kingdom, and even United States by migratory birds. Here, we tested currently used commercial clade 2.3.2 H5 vaccines to evaluate mortality, clinical signs, virus shedding, and histological damage after experimental infection of chickens with the clade 2.3.4.4 HPAI H5N8 virus. Although the vaccination protected chickens from death, it failed to prevent chickens from shedding the virus and from tissue damage according to histological examination. These results suggest that the use of appropriate vaccines that match the currently epidemic HPAI virus is recommended, and continuous HPAI surveillance and testing of currently used commercial vaccines should be performed.