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( Dan Li ),( Dongwei Fu ),( Yue Zhang ),( Xueling Ma ),( Liguo Gao ),( Xiaohua Wang ),( Dongpo Zhou ),( Kai Zhao ) 한국미생물생명공학회(구 한국산업미생물학회) 2017 Journal of microbiology and biotechnology Vol.27 No.8
The content of taxol in the bark of yews is very low, and this is not affordable from the environmental point of view. Thus, it is a necessity to look for alternative sources of taxol production to solve its supply. Currently, a large portion of the taxol in the market comes from chemical semi-synthesis, but the semi-synthetic precursors such as baccatin III and 10- deacetyl-baccatin III are extracted from needles and twigs of yew trees. Taxol-producing fungi as a renewable resource is a very promising way to increase the scale of taxol production. Our group has obtained a taxol-producing endophytic fungus, Aspergillus niger subsp. taxi HD86-9, to examine if A. niger can produce the taxanes. Six compounds from the fermentation broth of strain HD86-9 were isolated and identified by <sup>1</sup>H NMR, <sup>13</sup>C NMR, and ESI-MS. The results showed that the six compounds included four taxane diterpenoids (taxol, cephalomannine, baccatin III, and 10-deacetyl-baccatin III) and two non-taxane compounds (β-sitosterol and flavonoid isovitexin). The study verified that the taxanes can be produced by the A. niger, which is very important to taxol production via chemical semi-synthesis. Additionally, the finding is potentially very significant to solve the taxol semi-synthetic precursors extracted from needles and twigs of yew trees, and the precursor production can be easily increased through the culture condition optimization, genetic breeding, and metabolic engineering of the A. niger.
A New Model for Predicting the Retention of Stationary Phase in Countercurrent Chromatography
( Ye Li ),( Lishui Sun ),( Xueling Du ) 조선대학교 공학기술연구원 2010 공학기술논문지 Vol.3 No.4
The retention of stationary phase (Sf) is a crucial factor in countercurrent chromatography (CCC), which is used for the derivation of the column efficiency, peak resolution, and solute retention. Both operation conditions (flow-rate of mobile phase and rotation speed) and physical properties of two-phase systems (density difference and viscosity) exert important effects on Sf and have been studied to improve the efficiency in various CCC separations. In this study, an artificial neural network (ANN) was used to simultaneously predict the effects of operation conditions and physical properties of two-phase systems on the retention of stationary phase in CCC. The agreement between the predicted and the literature data, which contained the retention of stationary phase for 16 two-phase systems under different operation condition with 194 data points, was excellent with the total average absolute deviation (AAD) of 1.86% and the maximum deviation of 8.79%. By comparing the results predicted by ANN with the results calculated by the mathematical model proposed in literature, it was found that more accurate predictions were achieved by means of the ANN.
Measurement and Correlation of Solubility of Solanesol in Single and Binary Solvent Systems
( Ye Li ),( Xueling Du ),( Jingshun Chen ) 조선대학교 공학기술연구원 2010 공학기술논문지 Vol.3 No.3
The aim of this study was to investigate the solubilities of solanesol in three single solvent and three binary solvent systems at different temperatures ranging from 268.15 K to 303.15 K. The single solvent systems included methanol, ethanol, and acetonitrile. The binary solvent systems included methanol-ethanol, methanol-acetic ester, and ethanol-water. Two empirical equations were proposed to correlate with the experimental solubilities and the calculated results were well consistent with experimental data. The results may provide scientific references for the separation process of solanesol.
Rugang Yu,Xueling Du,Jing Li,Lan Liu,Chaomeng Hu,Xiaoling Yan,Yuqing Xia,Huijuan Xu 한국유전학회 2020 Genes & Genomics Vol.42 No.4
Background Taproot skin color is a major trait for assessing the commercial and nutritional quality of radish, and red-skinned radish is confirmed to improve consumer’s interest and health. However, little is known about the molecular mechanisms responsible for controlling the formation of red-skinned radish. Objective This study aimed to identify the differentially expressed anthocyanin biosynthetic genes between red- and whiteskinned radishes and understand the molecular regulatory mechanism underlying red-skinned radish formation. Methods Based on the published complete genome sequence of radish, the digital gene expression profiles of Yangzhouyuanbai (YB, white-skinned) and Sading (SD, red-skinned) were analyzed using Illumina sequencing. Results A total of 3666 DEGs were identified in SD compared with YB. Interestingly, 46 genes encoded enzymes related to anthocyanin biosynthesis and 241 genes encoded transcription factors were identified. KEGG pathway analysis showed that the formation of red-skinned radish was mainly controlled by pelargonidin-derived anthocyanin biosynthetic pathway genes. This process included the upregulation of PAL, C4H, 4CL, CHS, CHI, F3H, DFR, LDOX, and UGT enzymes in SD. CHS genes were specifically expressed in SD, and it might be the key point for red pigment accumulation in red-skinned radish. Furthermore, MYB1/2/75, bHLH (TT8), and WD 40 showed higher expression in SD than in YB. Meanwhile, the corresponding low-abundance anthocyanin biosynthesis enzymes and upregulation of MYB4 might be the factors influencing the formation of white-skinned radish. Conclusion These findings provide new insights into the molecular mechanisms and regulatory network of anthocyanin biosynthesis in red-skinned radish.
Qipeng Yuan,Ye Li,Xueling Du 한국화학공학회 2008 Korean Journal of Chemical Engineering Vol.25 No.6
The effects of key parameters on the preparation and regeneration of protoplast from the β-carotene-producing fungus Blakeslea trispora were discussed in this paper, including the combination of various enzymes, mycelial age, digesting time and temperature, pH value, osmotic stabilizers, pretreatment, culture medium and culture method. Under the condition of mixed enzymes in osmotic stabilizer (0.6 M NaCl) combined with 2% lysozyme, 3% cellulase and 3% snailase, the highest protoplast yield, as high as 7.48×106 protoplasts/mL, was obtained when mycelial age was 60 h at pH 5.0-6.0 with digesting for 14-16 h at 28 oC. After purification of the obtained protoplasts, they were regenerated in PDA regenerative medium using bilayer plate culture method. To validate the usability of the protoplasts, a novel plasmid with green fluorescent protein (GFP) was used in transformation for easy visual observation. The results showed that the protoplasts prepared by the optimized method were active and applicable in further gene manipulation experiments.
Ziya Lan,Huiling Huang,Chenyang Liu,Jincai Li,Xueling Li 한국식품과학회 2024 Food Science and Biotechnology Vol.33 No.6
Animal intestines is considered as a source of lactic acid bacteria (LAB) that have potential to decrease the nitrite level during fermentation of food such as pickles. It was hypothesized that optimized level of LAB has a high capacity to degrade nitrite during Chinese pickle fermentation and benefit a higher acceptability of the Chinese pickle product. This study aims to investigate the performance of a goose intestine-isolated LAB strain G6 under the species Lactiplantibacillus plantarum as a starter culture of Chinese pickles. The results showed that Lactiplantibacillus sp. G6 had a nitrite degradation rate close to 100% under the MRS broth condition of 25 °C, 2% inoculum volume and pH at 5. As a starter culture for Chinese pickle, this strain was able to achieve a higher LABs amount, lower nitrite residue after fermentation, compared with the group without the starter, which implicates its feasibility of applying on fermented food for reducing nitrite level.