The hydrogen storage nanomaterial MgH2 improves irradiation-induced male fertility impairment by suppressing oxidative stress

Jing Ma,Suhe Dong,Hongtao Lu,Zhongmin Chen,Huijie Yu,Xuejun Sun,Renjun Peng,Wei Li,Sinian Wang,Qisheng Jiang,Fengsheng Li,Li Ma 한국생체재료학회 2022 생체재료학회지 Vol.26 No.2

Objective: This study aimed to reveal the protective effect of hydrogen storage nanomaterial MgH2 on radiationinduced male fertility impairment. Methods: The characterization of MgH2 were analyzed by scanning electron microscopy (SEM) and particle size analyzer. The safety of MgH2 were evaluated in vivo and in vitro. The radioprotective effect of MgH2 on the reproductive system were analyzed in mice, including sperm quality, genetic effect, spermatogenesis, and hormone secretion. ESR, flow cytometry and western blotting assay were used to reveal the underlying mechanisms. Results: MgH2 had an irregular spherical morphology and a particle size of approximately 463.2 nm, and the content of Mg reached 71.46%. MgH2 was safe and nontoxic in mice and cells. After irradiation, MgH2 treatment significantly protected testicular structure, increased sperm density, improved sperm motility, reduced deformity rates, and reduced the genetic toxicity. Particularly, the sperm motility were consistent with those in MH mice and human semen samples. Furthermore, MgH2 treatment could maintain hormone secretion and testicular spermatogenesis, especially the generation of Sertoli cells, spermatogonia and round sperm cells. In vitro, MgH2 eliminated the [·OH], suppressed the irradiation-induced increase in ROS production, and effectively alleviated the increase in MDA contents. Moreover, MgH2 significantly ameliorated apoptosis in testes and cells and reversed the G2/M phase cell cycle arrest induced by irradiation. In addition, MgH2 inhibited the activation of radiation-induced inflammation and pyroptosis. Conclusion: MgH2 improved irradiation-induced male fertility impairment by eliminating hydroxyl free radicals.

An Improved Model of General Data Publish/Subscribe Based on Data Distribution Service

Shufen Liu,Xuejun Ma,Xinyong Wang 보안공학연구지원센터 2016 International Journal of Database Theory and Appli Vol.9 No.11

Most existing data publish/subscribe systems applied in a particular field, the lack of generality. In order to satisfy the general support for interdisciplinary model, proposed an improved model of general data publish/subscribe. The model supports the configuration and modification of the underlying data types. In order to avoid the impact on the application layer while changing the underlying DDS product, proposed an encapsulation on DDS based on the abstract factory pattern. Finally, through simulation experiments to verify the feasibility of the proposed model, the simulation results show that the improved model can be well applied to various types of data publish/subscribe occasions, with high performance.

Preparation of ursodeoxycholic acid by direct electro-reduction of 7-ketolithocholic acid

Xiangxi Yuan,Xuejun Cao,Xiaolei Ma 한국화학공학회 2014 Korean Journal of Chemical Engineering Vol.31 No.7

A novel method of producing ursodeoxycholic acid was developed through direct electro-reduction of 7-ketolithocholic acid in a divided electrolytic cell. Titanium ruthenium mesh electrode was used as the anode, whilehigh purity lead plate was used as the cathode. The process was optimized with regards to the electrolyte, temperature,concentration of methanol, current density and concentration of anolyte. When potassium bromide was used as theelectrolyte, the saturated solution of 7-ketolithocholic acid in 85-93% (v/v) methanol, current density 9.52-28.6 A/m2and concentration of anolyte at 4-6% (w/w), the maximum percentage yield of ursodeoxycholic acid could be 47%. The method will provide a potential approach for large-scale production of ursodeoxycholic acid.

Power Sharing Strategy of A Series-parallel-connected EV Charger

Guozhen Hu,Huang Lei,Xuejun Ma,Guorong Zhu 전력전자학회 2015 ICPE(ISPE)논문집 Vol.2015 No.6

In order to meet different requirements of rechargeable batteries in Electric vehicles, a charger with hybrid structure, which designed as module and combined in series and parallel, was proposed in this paper. Each individual module uses ZCS phase-shifted full-bridge converters. And in order to ensure power sharing of each module, the outer loop power control strategy based on average current sharing method has been adopted. A combination prototype was built following the proposed strategy. Experimental results verify the correctness of the proposed scheme and the performance of power sharing system can meet the charging application.

Comparison of Survival Rates between Chinese and Thai Patients with Breast Cancer

Che, Yanhua,You, Jing,Zhou, Shaojiang,Li, Li,Wang, Yeying,Yang, Yue,Guo, Xuejun,Ma, Sijia,Sriplung, Hutcha Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.15

The burden and severity of a cancer can be reflected by patterns of survival. Breast cancer prognosis between two countries with a different socioeconomic status and cultural beliefs may exhibit wide variation. This study aimed to describe survival in patients with breast cancer in China and Thailand in relation to demographic and clinical prognostic information. Materials and Methods: We compared the survival of 1,504 Chinese women in Yunnan province and 929 Thai women in Songkhla with breast cancer from 2006 to 2010. Descriptive prognostic comparisons between the Chinese and Thai women were performed by relative survival analysis. A Cox regression model was used to calculate the hazard ratios of death, taking into account the age, disease stage, period of diagnosis and country. Results: The overall 5-year survival proportion for patients diagnosed with breast cancer for Yunnan province (0.72) appeared slightly better than Songkhla (0.70) without statistical significance. Thai women diagnosed with distant and regional breast cancer had poorer survival than Chinese women. Disease stage was the most important determinant of survival from the results of Cox regression model. Conclusions: Breast cancer patients in Kunming had slightly greater five-year survival rate than patients in Songkhla. Both Chinese and Thai women need improvement in prognosis, which could conceivably be attained through increased public education and awareness regarding early detection and compliance to treatment protocols.

Ginsenoside Rh2 reduces depression in offspring of mice with maternal toxoplasma infection during pregnancy by inhibiting microglial activation via the HMGB1/TLR4/NF-kB signaling pathway

Xiang Xu,Yu-Nan Lu,Jia-Hui Cheng,Hui-Wen Lan,Jing-Mei Lu,Guang-Nan Jin,Guang-Hua Xu,Cheng-Hua Jin,Juan Ma,Hu-Nan Piao,Xuejun Jin,Lian-Xun Piao 고려인삼학회 2022 Journal of Ginseng Research Vol.46 No.1

Background: Maternal Toxoplasma gondii (T. gondii) infection during pregnancy has been associated with various mental illnesses in the offspring. Ginsenoside Rh2 (GRh2) is a major bioactive compound obtained from ginseng that has an anti-T. gondii effect and attenuates microglial activation through toll-like receptor 4 (TLR4)/nuclear factor-kappa B (NF-kB) signaling pathway. GRh2 also alleviated tumor-associated or lipopolysaccharide-induced depression. However, the effects and potential mechanisms of GRh2 on depression-like behavior in mouse offspring caused by maternal T. gondii infection during pregnancy have not been investigated. Methods: We examined GRh2 effects on the depression-like behavior in mouse offspring, caused by maternal T. gondii infection during pregnancy, by measuring depression-like behaviors and assaying parameters at the neuronal and molecular level. Results: We showed that GRh2 significantly improved behavioral measures: sucrose consumption, forced swim time and tail suspended immobility time of their offspring. These corresponded with increased tissue concentrations of 5-hydroxytryptamine and dopamine, and attenuated indoleamine 2,3-dioxygenase or enhanced tyrosine hydroxylase expression in the prefrontal cortex. GRh2 ameliorated neuronal damage in the prefrontal cortex. Molecular docking results revealed that GRh2 binds strongly to both TLR4 and high mobility group box 1 (HMGB1). Conclusion: This study demonstrated that GRh2 ameliorated the depression-like behavior in mouse offspring of maternal T. gondii infection during pregnancy by attenuating the excessive activation of microglia and neuroinflammation through the HMGB1/TLR4/NF-kB signaling pathway. It suggests that GRh2 could be considered a potential therapy in preventing and treating psychiatric disorders in the offspring mice of mothers with prenatal exposure to T. gondii infection.