Pathway and Network Analysis in Glioma with the Partial Least Squares Method

Gu, Wen-Tao,Gu, Shi-Xin,Shou, Jia-Jun Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.7

Gene expression profiling facilitates the understanding of biological characteristics of gliomas. Previous studies mainly used regression/variance analysis without considering various background biological and environmental factors. The aim of this study was to investigate gene expression differences between grade III and IV gliomas through partial least squares (PLS) based analysis. The expression data set was from the Gene Expression Omnibus database. PLS based analysis was performed with the R statistical software. A total of 1,378 differentially expressed genes were identified. Survival analysis identified four pathways, including Prion diseases, colorectal cancer, CAMs, and PI3K-Akt signaling, which may be related with the prognosis of the patients. Network analysis identified two hub genes, ELAVL1 and FN1, which have been reported to be related with glioma previously. Our results provide new understanding of glioma pathogenesis and prognosis with the hope to offer theoretical support for future therapeutic studies.

Fluid-structure interaction analysis of heat exchanger with torsional flow in the shell side

Xin Gu,Guan Wang,Qianxin Zhang,Cheng Chen,Ning Li,Weijie Chen 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.1

Based on the theory of fluid-structure interaction (FSI), the flow characteristics and mechanical properties of torsional flow heat exchanger (TFHX) and cross torsional flow heat exchanger (CTFHX) were numerically studied. The simulation results were compared with the experimental results to verify the reliability of the numerical simulation. The results show that the pressure drop of CTFHX decrease is 30.31-32.56 % lower than that of TFHX, and the heat transfer coefficient is found to lower by 16.8-18.5 %, but the comprehensive performance h/∆P is increased by 14.8-17.9 %. There is also a higher stress around the baffle holes, and the influence of temperature load on stress is much greater than that of pressure load. Moreover, the linearization results of hazardous locations show that CTFHX has greater stress. This study provides theoretical guidance for the structural optimization and equipment maintenance of heat exchanger.

VR 환경 속에서 색온도 및 조도(照度)가 환경인지도와 길찾기에 미치는 영향

고흔명(Xin Ming Gu),이일남(Yi Nan Li) 한국디자인리서치학회 2022 한국디자인리서치 Vol.7 No.4

본 연구의 목적은 가상 환경에서 조명의 색온도, 광속(lx) 조건이 인간의 길찾기 및 환경지각에 대한 주관적 판단에미치는 영향을 탐구하는 데에 있다. 또한 CG 가상현실 환경을 통해 복잡한 평면도와 지속적 공간이 있는 가상 환경에서 색온도, 광색 및 광속의 변량을 이용하여 가상 환경의 인식 및 길찾기 능력을 해결하고 향상하게 시키는 방법을 연구하였다. 다양한 광색 조건 변수의 가상현실 환경에서 실험 참가자(N=60)의 자료를 조사하고 SD의 심리적조사 방법을 사용하여 이러한 조건 아래의 차이를 평가하였다. 그 결과 고(高)색 온도 및 중(中) 색온도의 광원 환경보다 저색 온도(난색)의 흡인력과 기억 분수가 더 높다. 하지만 고색 온도(한색) 환경은 선택의 망설임을 줄이고 더나은 통과 효율을 얻을 수 있는 것으로 나타났다. 또한 연구에 따르면 고아속이 낮은 가상 환경에서 고색 온도를 사용하든 저색 온도를 사용하든 다 부정적인 효과를 낼 것이라고 한다. 따라서 저색 온도 조명 환경은 효과적인 도로찾기 표시로 사용될 수 있고, 광범위적으로 고색 온도를 사용하는 공간 조명 조건이 도로 찾기 및 통행 효율에 더도움이 되는 것으로 간주한다. 또 여성이 남성보다 고광도 수준의 가상 환경을 선호한다는 연구 결과도 나왔다. The purpose of this study is to investigate the influence of color temperature and lx conditions on human pathfinding and environmental perception in virtual environment.In addition, in CG virtual reality environment, how to use color temperature, light color and light flux variables to solve and improve the cognitive and pathfinding ability of virtual environment is studied.The data of participants (N=60) in virtual reality environment with various light and color conditions were investigated, and the differences were evaluated using SD psychological survey method.The results show that low color temperature (warm color) has higher attraction and memory score than high color temperature and neutral color temperature.Furthermore, the study confirmed that the use of color temperature in a virtual environment with low-throughput light levels, regardless of color temperature, results in negative effects.Therefore, low color temperature (warm color) lighting environment can be used as an effective wayfinding landmark, but wider use of high color temperature (cold color) lighting conditions are considered to be more conducive to pathfinding and traffic efficiency.In addition, the results show that women prefer high-brightness virtual environments to men.

Semi-vioxanthin Isolated from Marine-Derived Fungus Regulates Tumor Necrosis Factor-α, Cluster of Differentiation (CD) 80, CD86, and Major Histocompatibility Complex Class II Expression in RAW264.7 Cells <i>via</i> Nuclear Factor-κB and Mitogen-Activat

Yang, Xin-Ying,Cai, Sheng-Xin,Zhang, Wen-Ji,Tang, Xue-Lian,Shin, Hye-Young,Lee, Joo-Young,Gu, Qian-Qun,Park, Hyun Pharmaceutical Society of Japan 2008 BIOLOGICAL & PHARMACEUTICAL BULLETIN Vol.31 No.12

<P>Semi-vioxanthin isolated from marine-derived fungus was assessed for immunoregulatory activity in mouse RAW264.7 macrophages. In the present study, the facilitative effects of semi-vioxanthin on tumor necrosis factor-α (TNF-α) and its mRNA expression and on expression of the co-stimulatory molecules, cluster of differentiation (CD) 80, CD86 and major histocompatibility complex class II (MHC II), as well as the molecular mechanism underlying the immunologic enhancement properties of semi-vioxanthin were studied. Our results clearly indicated that semi-vioxanthin treatment resulted in the degradation of IκBα, which led to the activation and nuclear translocation of the p65 subunit of nuclear factor-κB (NF-κB), as determined by immunoblotting, immunofluorescence and electrophoretic mobility shift assays (EMSA). Moreover, TNF-α production was prevented by NF-κB and mitogen-activated protein kinase (MAPK) inhibitors. Inhibition of NF-κB and extracellular signal regulated kinases (ERK1/2) activity by specific inhibitors blunted the effect of semi-vioxanthin on the up-regulation of CD80, CD86 and MHCII expression, but neither p38 MAPK nor c-Jun N-terminal kinase (JNK) inhibitor had this effect. Thus, we demonstrate that semi-vioxanthin regulates TNF-α production through NF-κB and MAPK signaling pathways. Activation of NF-κB and ERK1/2 were necessary for CD80, CD86 and MHCII expression induced by semi-vioxanthin. These data suggest that semi-vioxanthin has immunoregulatory effects.</P>

Thalidomide Combined with Chemotherapy in Treating Patients with Advanced Colorectal Cancer

Huang, Xin-En,Yan, Xiao-Chun,Wang, Lin,Ji, Zhu-Qing,Li, Li,Liu, Meng-Yan,Qian, Ting,Shen, Hui-Ling,Gu, Han-Gang,Liu, Yong,Gu, Ming,Deng, Li-Chun Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.17

Objective: To assess the safety and effectiveness of thalidomide (produced by CHANGZHOU PHARMACEUTICAL FACTORY CO.LTD) combined with chemotherapy in treating patients with advanced colorectal cancer. Method: A consecutive cohort of pretreated patients with advanced colorectal cancer were treated with thalidomide combined with chemotherapy. And chemotherapy for patients with advanced colorectal cancer were administered according to the condition of patients. Thalidomide was orally administered at a dosage of 50mg/day to 150mg/day before sleeping for at least 14 days. After at least 14 days of treatment, safety and side effects were evaluated. Results: There were 12 female and 3 male patients with advanced cancer recruited into this study, including 9 patients with colon, 6 patients with rectal cancer. The median age of patients was 57(41-82) years. Partial response was observed in 2 patients (2/15), and stable disease in 3 patients(3/15). Incidences of Grade 1 to 2 myelosuppression was observed in 1/15 patients, and Grade 1 to 2 elevation of hepatic enzyme was recorded in 1/15 patients. Adverse effects on the gastrointestinal tract were documented in 1/15 patients, and were Grade 1. No Grade 3-4 toxicities were diagnosed. No treatment related death was found. Conclusions: Thalidomide combined with chemotherapy was safe and mildly effective in treating patients with advanced colorectal cancer. However, further study should be conducted to clarify the effectiveness of this combination.

Transcriptome characterization and gene expression analysis related to immune response in Gynaephora qinghaiensis pupae

Hai-Zhen Wang,Xin Zhong,Gu-Ren Zhang,Xin Liu,Li Gu 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.2

Gynaephora qinghaiensis is a pest on the Qinghai-Tibet Plateau (QTP) that has led to substantial destruction of grassland vegetation. Its pupae are also natural hosts of parasitic wasp. Sexual dimorphism in immune responses is prevalent in vertebrates and invertebrates. However, sexual dimorphism in immune responses of insects, particularly G. qinghaiensis, is poorly understood at the transcriptional level. Here, we performed transcriptome sequencing in male and female pupae of G. qinghaiensis. A total of 118,357,040 clean reads were obtained and assembled into 114,944 unigenes. To explore the difference of immune responses in pupae of both sexes (male and female) of G. qinghaiensis. based on transcriptional level, we characterized the expression profiles of candidate transcripts in the two sexes of G. qinghaiensis. A total of 3,469 unigenes (1,888 up-regulated and 1,581 down-regulated genes) were differentially expressed in pupae of both sexes. Among the differentially expressed genes (DEGs), 263 unigenes related to immune responses were found in the two sexes in G. qinghaiensis; of these, 202 were up-regulated and 61 were down-regulated in the female pupae compared to male pupae. This indicates there were some differences in the expression of immune-related genes between male and female G. qinghaiensis pupae. Therefore, we speculated that the differences in immune responses also likely exsited in pupae of both sexes. Our report provides a valuable genomic resource for further studies of Gynaephora and improves our understanding of the molecular mechanisms underlying immunological differences between male and female insects.

A Novel Mutation p.L461P in KRT5 Causing Localized Epidermolysis Bullosa Simplex

( Xin Jiang ),( Yingyu Zhu ),( Huihui Sun ),( Feng Gu ) 대한피부과학회 2021 Annals of Dermatology Vol.33 No.1

Background: Epidermolysis bullosa (EB) is a rare genetic disease with widely different clinical manifestations, but the relationship between genotype and phenotype is not fully understood. In the present study, we recruited a Chinese family in which two members had been diagnosed with localized EB simplex (EBS), with clinical manifestation, including blisters and erosions on the soles of the feet since infancy. Objective: To identify and confirm the genetic variation in a Chinese family diagnosed as localized EBS. Methods: Our study included two patients, other healthy members of the family, and 100 normal controls. Genomic DNA samples were isolated from each participant, and then polymerase chain reaction (PCR) direct sequencing was performed. Results: The results of PCR direct sequencing revealed a novel heterozygous missense mutation in codon 461 of exon 7 of KRT5 (c.1382T>C), which led to an amino acid change (p.L461P) in the patients with EBS but was absent in unaffected family members and 100 unrelated control samples. Conclusion: The present study broadens the mutational spectrum of EBS, and this knowledge could be harnessed for prenatal screening, gene diagnosis, and gene therapy for localized EBS. (Ann Dermatol 33(1) 11∼17, 2021)

Quality of Life and Radiotherapy Complications of Chinese Nasopharyngeal Carcinoma Patients at Different 3DCRT Stages

Gu, Mo-Fa,Su, Yong,Chen, Xin-Lin,He, Wei-Ling,He, Zhen-Yu,Li, Jian-Jun,Chen, Miao-Qiu,Mo, Chuan-Wei,Xu, Qian,Diao, Yuan-Ming Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.1

Purpose: the study aimed to compare the quality of life (QOL) and radiotherapy complications among Chinese nasopharyngeal carcinoma (NPC) patients at different 3-dimensional conformal radiotherapy (3DCRT) stages adjusting for other variables. Methods: 511 NPC patients at different 3DCRT stages were enrolled. They were interviewed regarding SF-36, complications and socio-demographic variables and cancer- or treatment-related variables. Analysis of covariance (ANCOVA) based on SF-36, complications scores as dependent variables, 3DCRT stages as independent variables, and other variables as covariate were established. Results: The influencing factors of PCS included 3DCRT stages and age group. The influencing factors of MCS included 3DCRT stages and income. Most QOL scores of NPC patients were significantly associated with 3DCRT stage, after accounting for other variables. QOL scores of the patients receiving 3DCRT were the lowest, QOL scores of people after 3DCRT gradually increased. PCS scores of people greater than 5 years after 3DCRT was improved to or even better than the level before 3DCRT. The complications with significantly different scores of patients at different 3DCRT status included xerostomia, throat ache, hypogeusia, caries, hearing loss, snuffles. Conclusions: Clinicians should pay more attention to older NPC patients and patients with lower income. When patients receive 3DCRT, measures should be taken to reduce radiation injury to improve the patients' QOL.

Current Status of Hygiene Standard Setting for Chemical Substances in the Workplace in China

Gu Xue-qi,Liang, You-xin 가톨릭대학산업의학센타 산업의학연구소 1991 韓國의 産業醫學 Vol.30 No.3

Sirolimus and Non-melanoma Skin Cancer Prevention after Kidney Transplantation: A Meta-analysis

Gu, Yu-Hong,Du, Jia-Xin,Ma, Man-Ling Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.9

Background: Whether sirolimus is useful in the prevention of non-melanoma skin cancer (NMSC) remains unclear and we therefore performed this meta-analysis of randomized controlled trials to test the hypothesis that Sirolimus-based immunosuppression is associated with a decrease in NMSC. Methods: The main outcomes were NMSC, squamous-cell carcinoma and basal-cell carcinoma. The pooled risk ratio (RR) with its 95% confidence interval (95%CI) were used to assess the effects. Results: 5 randomized trials involving a total of 1499 patients receiving kidney transplantation were included. Patients undergoing Sirolimus-based immunosuppression had much lower risk of NMSC (RR = 0.49, 95%CI 0.32-0.76, P = 0.001). Subgroup analyses by tumor type showed that Sirolimus-based immunosuppression significantly decreased risk of both squamous-cell carcinoma (RR = 0.58, 95%CI 0.43-0.78, P < 0.001) and basal-cell carcinoma (RR = 0.56, 95%CI 0.37-0.85, P = 0.006). The quality of evidence was high for NMSC, and moderate for squamous-cell carcinoma and basal-cell carcinoma. No evidence of publication bias was observed. Conclusion: High quality evidence suggests that Sirolimus-based immunosuppression decreases risk of non-melanoma skin cancer, and Sirolimus has an antitumoral effect among kidney-transplant recipients.