Effects of L-arginine on Endothelium Derived Factors and Cyclic Nucleotides in Broilers under Low Ambient Temperature

Han, Bo,Yoon, Soonseek,Han, Hongryul,Wang, Xiaolong Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.11

A flock of AA breed chickens were reared in peterstme brood-vait chamber and were provided with high energy pelleted feed. At 14 d of age, a total of 350 birds were randomly divided into 3 groups as follows: 100 birds were exposed to normal ambient temperature of 20$^{\circ}C$ for control group; 150 birds were exposed to lower ambient temperature of 11$^{\circ}C$ to induce ascites (treatment I); and another group of 100 birds were exposed to lower ambient temperature of 11$^{\circ}C$ and fed diet containing 1% L-arginine for ascitic prophylactic treatment (treatment II). Samples were collected from blood and abdominal fluid of chicken at 3, 4, 5, 6 and 7 wk of age subsequently, to analysis the contents of plasma endothelin (ET-1), angiotensin II (Ang II), cyclic adenosine monophosphate (cAMP), cyclic guanosine monophosphate (cGMP). The results indicated that the contents of cAMP, cGMP, and Ang II in reatment I and ascitic broilers were higher than the corresponding control group (p<0.01, p<0.05), ET-1 of preascitic broilers were control group (p<0.05), while there was an insignificant difference with later ascitic broilers. The contents of cAMP and cGMP in treatment II were higher than the treatment I and control groups (p<0.01, p<0.05), whereas, the contents of Ang II were gradually decreased compared to the control group (p<0.05), the contents of ET-1 were insignificantly different. On further analysis, the increased plasma Ang II at low ambient temperature condition in broilers made endothelium cell secretion of increased ET-1, cAMP, cGMP and decreased NO. Therefore, low temperature accelerated ascites syndrome in broilers. Supplemently L-arginine can decrease ET-1, and increase cAMP and cGMP. It is concluded that cAMP mediated in broilers pulmonary hypertension syndrome.

The Secure IEEE 11073:20601 Protocol and Agent Structure for Protecting PHD

Xiaolong Xu,Zhonghe Gao,Lijuan Han 보안공학연구지원센터 2016 International Journal of Security and Its Applicat Vol.10 No.12

In this paper, we suggest improvements of security in IEEE 11073:20601 protocol and the structure of its agent for protecting user’s healthcare information safely. Current IEEE 11073:20601 standard does not provide any method to ensure security of data exchange, and it assumes that data exchange is secured by other means. The suggested improvements include mechanism to enhance security in exchanging and storing data in PHD. While providing those advantages, it also satisfies availability by effective encoding rules and data protecting algorithm.

Atomic insights into effects of grain boundary types on stress corrosion cracking of Ni60Cr30Fe10 alloy in the high-temperature water

Xiaolong Liu,Woonggi Hwang(황웅기),Boyoung Lee(이보영),Sung-Yup Kim(김성엽),Sangsoo Han(한상수) 대한용접·접합학회 2017 대한용접학회 특별강연 및 학술발표대회 개요집 Vol.2017 No.11

Morphology and Electrochemical Behavior of Anodic Oxide Films of Ti in Aqueous H₃PO₄

Xiaolong ZHU,Kyo-Han KIM 대한치과재료학회 2000 대한치과기재학회 학술대회 Vol.2000 No.1

Isolation and identification of goose skeletal muscle satellite cells and preliminary study on the function of C1q and tumor necrosis factor-related protein 3 gene

Wang Han,He Ke,Zeng Xuehua,Zhou Xiaolong,Yan Feifei,Yang Songbai,Zhao Ayong 아세아·태평양축산학회 2021 Animal Bioscience Vol.34 No.6

Objective: Skeletal muscle satellite cells (SMSCs) are significant for the growth, regeneration, and maintenance of skeletal muscle after birth. However, currently, few studies have been performed on the isolation, culture and inducing differentiation of goose muscle satellite cells. Previous studies have shown that C1q and tumor necrosis factor-related protein 3 (CTRP3) participated in the process of muscle growth and development, but its role in the goose skeletal muscle development is not yet clear. This study aimed to isolate, culture, and identify the goose SMSCs in vitro. Additionally, to explore the function of CTRP3 in goose SMSCs. Methods: Goose SMSCs were isolated using 0.25% trypsin from leg muscle (LM) of 15 to 20 day fertilized goose eggs. Cell differentiation was induced by transferring the cells to differentiation medium with 2% horse serum and 1% penicillin streptomycin. Immunofluorescence staining of Desmin and Pax7 was used to identify goose SMSCs. Quantitative realtime polymerase chain reaction and western blot were applied to explore developmental expression profile of CTRP3 in LM and the regulation of CTRP3 on myosin heavy chains (MyHC), myogenin (MyoG) expression and Notch signaling pathway related genes expression. Results: The goose SMSCs were successfully isolated and cultured. The expression of Pax7 and Desmin were observed in the isolated cells. The expression of CTRP3 decreased significantly during leg muscle development. Overexpression of CTRP3 could enhance the expression of two myogenic differentiation marker genes, MyHC and MyoG. But knockdown of CTRP3 suppressed their expression. Furthermore, CTRP3 could repress the mRNA level of Notch signaling pathway-related genes, notch receptor 1, notch receptor 2 and hairy/ enhancer-of-split related with YRPW motif 1, which previously showed a negative regulation in myoblast differentiation. Conclusion: These findings provide a useful cell model for the future research on goose muscle development and suggest that CTRP3 may play an essential role in skeletal muscle growth of goose. Objective: Skeletal muscle satellite cells (SMSCs) are significant for the growth, regeneration, and maintenance of skeletal muscle after birth. However, currently, few studies have been performed on the isolation, culture and inducing differentiation of goose muscle satellite cells. Previous studies have shown that C1q and tumor necrosis factor-related protein 3 (CTRP3) participated in the process of muscle growth and development, but its role in the goose skeletal muscle development is not yet clear. This study aimed to isolate, culture, and identify the goose SMSCs <i>in vitro</i>. Additionally, to explore the function of CTRP3 in goose SMSCs.Methods: Goose SMSCs were isolated using 0.25% trypsin from leg muscle (LM) of 15 to 20 day fertilized goose eggs. Cell differentiation was induced by transferring the cells to differentiation medium with 2% horse serum and 1% penicillin streptomycin. Immunofluorescence staining of Desmin and Pax7 was used to identify goose SMSCs. Quantitative realtime polymerase chain reaction and western blot were applied to explore developmental expression profile of CTRP3 in LM and the regulation of CTRP3 on myosin heavy chains (MyHC), myogenin (MyoG) expression and Notch signaling pathway related genes expression.Results: The goose SMSCs were successfully isolated and cultured. The expression of <i>Pax7</i> and Desmin were observed in the isolated cells. The expression of CTRP3 decreased significantly during leg muscle development. Overexpression of CTRP3 could enhance the expression of two myogenic differentiation marker genes, <i>MyHC</i> and <i>MyoG</i>. But knockdown of CTRP3 suppressed their expression. Furthermore, CTRP3 could repress the mRNA level of Notch signaling pathway-related genes, notch receptor 1, notch receptor 2 and hairy/enhancer-of-split related with YRPW motif 1, which previously showed a negative regulation in myoblast differentiation.Conclusion: These findings provide a useful cell model for the future research on goose muscle development and suggest that CTRP3 may play an essential role in skeletal muscle growth of goose.

Atomic insights into effects of cold work on stress corrosion cracking of Ni60Cr30Fe10 alloy in the high-temperature water

Liu Xiaolong,Woonggi Hwang(황웅기),Boyoung Lee(이보영),Sung-Yup Kim(김성엽),Sangsoo Han(한상수) 대한용접·접합학회 2017 대한용접학회 특별강연 및 학술발표대회 개요집 Vol.2017 No.11

Morphology and Electrochemical Behavior of Anodic Oxide Films of Ti in Aqueous H₃PO₄

KIM,Kyo-Han,ZHU,Xiaolong 대한치과기재학회 2000 대한치과재료학회지 Vol.27 No.1

Anodic oxidation is a superior method for the modification of titanium to promote the bonding between the implants and host tissue, including the improvements of mechanical interlocking and chemical bonding. Till now no systematic investigations have been reported on the anodization of titanium in H_3PO_4 solution.

One Step Fabrication of Micro-hole on SiCp/Al Cambered Surface by Micro-EDM and Mechanical-reaming Combined Machining

Sirui Gong,Han Wang,Xiaolong He,Zhenlong Wang,Yukui Wang 한국정밀공학회 2023 International Journal of Precision Engineering and Vol.10 No.4

SiCp/Al, as a new material, is widely used for its excellent wear resistance, corrosion resistance and other properties. Micro-holes are important but difficult to obtain on this ceramic material especially in curved surface. In this paper, taking micro bearing bush as an example, a micro-EDM and in-situ micro-reaming method is put forward for preparing micro through holes and micro eccentric holes on SiCp/Al cambered surface, without secondary clamping or tool change. High machining efficiency, low wear and no recasting layer can be achieved by this method. Here, machining characteristics is studied in hole micro-EDM on SiCp/Al, small taper and high efficiency machining is realized by comparing four electrical discharge drilling technique. Carbon deposition and tool wear are related to electrical parameters according to experimental exploration. After determining the discharge gap, taper and recast layer thickness, a segmented tool is designed, which can use the lower part to perform micro-EDM with the taper of 1.04°, MRR is 0.069 mm3/min, and the TWR is 3.22%. Then only needs to continue feeding the tool, the upper part of the tool can be used to achieve reaming, which can fully remove the recast layer, and decreases the roughness of hole wall from Ra 1.452 μm to Ra 0.803 μm.

A Novel Modulation Strategy for Diode-assisted Buck-boost VSI with Maximum Voltage Gain and Minimum Switching Frequency

Yan Zhang,Xiaolong Ma,Jinjun Liu,Zhuo Dong,Shah Zaman,Jiuqiang Han 전력전자학회 2015 ICPE(ISPE)논문집 Vol.2015 No.6

Diode-assisted buck-boost voltage source inverter (VSI) boosts the dc source voltage by introducing a diode-capacitor network. With capacitive charging in parallel and discharging in series, it extends voltage regulation range and avoids extreme boost duty ratio. As for the unique structure, various pulse width modulation (PWM) strategies are provided with regarding to the chopped intermediate dc-link voltage. In order to maximize voltage gain, as well as to achieve the increased efficiency, this paper proposed a novel modulation strategy by reducing the switching frequency of power devices in the inverter bridge. The operation principle and relationship of switching device voltage stress versus voltage gain are analyzed in detail. Simulation and experiments are implemented to verify the theoretical analysis. Compared with existing modulation strategies, diode-assisted buck? boost VSI with new modulation scheme demonstrates good performances: less power device requirement and higher efficiency in high voltage gain application. Owing to improved performance, it is a more competitive topology for wide range dc/ac voltage regulation in renewable energy application, especially for ac output with relatively high fundamental frequency. Furthermore, the idea of proposed modulation scheme can also be extended to the control of other quasi two-stage inverter.

An Improved Control Strategy for Z-source Inverter with Maximum Boost Capability and Minimum Switching Frequency

Yan Zhang,Xiaolong Ma,Jinjun Liu,Zhuo Dong,Jiuqiang Han 전력전자학회 2015 ICPE(ISPE)논문집 Vol.2015 No.6

Z-source inverter introduces a unique Xshaped impedance network and boosts the input voltage by utilizing shoot-through (ST) states. It provides a competitive single-stage approach for DC to AC power conversion in relatively low voltage boost ratio range (1?2). This paper proposes a novel control strategy for Z-source inverter. With this strategy, only one phase leg operates at high switching frequency to achieve both ST duty ratio control and output voltage regulation, while the other two phase legs maintain the fixed switching states during one sextant. Therefore, it reduces the equivalent switching frequency of power devices in the inverter bridge to about 1/3. However, it introduces the low frequency current and voltage ripple in the inductor and capacitor which related to the fundamental frequency of output voltage. It has the potential to achieve higher efficiency especially in high-switching-frequency and medium-frequency DC-AC power application. Then, the paper provides a detailed close-loop controller design approach for the novel modulation strategy. Furthermore, the detailed comparisons among the existing strategies and proposed control strategy are conducted in theory. Finally, all the theoretical analysis and calculation are verified by simulation results.