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Yang Zhuqing,Bao Linbin,Song Wanming,Zhao Xianghui,Liang Huan,Yu Mingjin,Qu Mingren 아세아·태평양축산학회 2024 Animal Bioscience Vol.37 No.2
Objective: The aim of this study was to investigate the impact of dietary nicotinic acid (NA) on apparent nutrient digestibility, rumen fermentation, and rumen microbiota in uncastrated Xiangzhong black cattle. Methods: Twenty-one uncastrated Xiangzhong black cattle (385.08±15.20 kg) aged 1.5 years were randomly assigned to the control group (CL, 0 mg/kg NA in concentrate diet), NA1 group (800 mg/kg NA in concentrate diet) and NA2 group (1,200 mg/kg NA in concentrate diet). All animals were fed a 60% concentrate diet and 40% dried rice straw for a 120-day feeding experiment. Results: Supplemental NA not only enhanced the apparent nutrient digestibility of acid detergent fiber (p<0.01), but also elevated the rumen acetate and total volatile fatty acid concentrations (p<0.05). 16S rRNA gene sequencing analysis of rumen microbiota revealed that dietary NA changed the diversity of rumen microbiota (p<0.05) and the abundance of bacterial taxa in the rumen. The relative abundances of eight Erysipelotrichales taxa, five Ruminococcaceae taxa, and five Sphaerochaetales taxa were decreased by dietary NA (p< 0.05). However, the relative abundances of two taxa belonging to Roseburia faecis were increased by supplemental 800 mg/kg NA, and the abundances of seven Prevotella taxa, three Paraprevotellaceae taxa, three Bifidobacteriaceae taxa, and two operational taxonomic units annotated to Fibrobacter succinogenes were increased by 1,200 mg/kg NA in diets. Furthermore, the correlation analysis found significant correlations between the concentrations of volatile fatty acids in the rumen and the abundances of bacterial taxa, especially Prevotella. Conclusion: The results from this study suggest that dietary NA plays an important role in regulating apparent digestibility of acid detergent fiber, acetate, total volatile fatty acid concentrations, and the composition of rumen microbiota.
Li, Xianghui,Zhen, Zhilei,Tang, Guodong,Zheng, Chong,Yang, Guofu Korean Society for Molecular and Cellular Biology 2016 Molecules and cells Vol.39 No.2
As a degenerative joint disease, osteoarthritis (OA) constitutes a major cause of disability that seriously affects the quality of life of a large population of people worldwide. However, effective treatment that can successfully reverse OA progression is lacking until now. The present study aimed to determine whether two small non-coding RNAs miR-29a and miR-140, which are significantly down-regulated in OA, can be applied together as potential therapeutic targets for OA treatment. MiRNA synergy score was used to screen the miRNA pairs that potentially synergistically regulate OA. An in vitro model of OA was established by treating murine chondrocytes with IL-$1{\beta}$. Transfection of miR-29a and miR-140 via plasmids was investigated on chondrocyte proliferation and expression of nine genes such as ADAMTS4, ADAMTS5, ACAN, COL2A1, COL10A1, MMP1, MMP3, MMP13 and TIMP metallopeptidase inhibitor 1 (TIMP1). Western blotting was used to determine the protein expression level of MMP13 and TIMP1, and ELISA was used to detect the content of type II collagen. Combined use of miR-29a and miR-140 successfully reversed the destructive effect of IL-$1{\beta}$ on chondrocyte proliferation, and notably affected the MMP13 and TIMP1 gene expression that regulates extracellular matrix. Although co-transfection of miR-29a and miR-140 did not show a synergistic effect on MMP13 protein expression and type II collagen release, but both of them can significantly suppress the protein abundance of MMP13 and restore the type II collagen release in IL-$1{\beta}$ treated chondrocytes. Compared with single miRNA transfection, cotransfection of both miRNAs exceedingly abrogated the suppressed the protein production of TIMP1 caused by IL-$1{\beta}$, thereby suggesting potent synergistic action. These results provided1novel insights into the important function of miRNAs' collaboration in OA pathological development. The reduced MMP13, and enhanced TIMP1 protein production and type II collagen release also implies that miR-29a and miR-140 combination treatment may be a possible treatment for OA.
Guofu Yang,Xianghui Li,Zhilei Zhen,Guodong Tang,Chong Zheng 한국분자세포생물학회 2016 Molecules and cells Vol.39 No.2
As a degenerative joint disease, osteoarthritis (OA) constitutes a major cause of disability that seriously af-fects the quality of life of a large population of people worldwide. However, effective treatment that can successfully reverse OA progression is lacking until now. The present study aimed to determine whether two small non-coding RNAs miR-29a and miR-140, which are significantly down-regulated in OA, can be applied together as potential therapeutic targets for OA treatment. MiRNA synergy score was used to screen the miRNA pairs that potentially synergistically regulate OA. An in vitro model of OA was established by treating murine chondrocytes with IL-1. Transfection of miR-29a and miR-140 via plasmids was investigated on chondrocyte proliferation and expression of nine genes such as ADAMTS4, ADAMTS5, ACAN, COL2A1, COL10A1, MMP1, MMP3, MMP13 and TIMP metallopeptidase inhibitor 1 (TIMP1). Western blotting was used to determine the protein expression level of MMP13 and TIMP1, and ELISA was used to detect the content of type II collagen. Combined use of miR-29a and miR-140 successfully reversed the destructive effect of IL-1 on chondrocyte proliferation, and notably affected the MMP13 and TIMP1 gene expression that regulates extracellular matrix. Although co-transfection of miR-29a and miR-140 did not show a synergistic effect on MMP13 protein expression and type II collagen release, but both of them can significantly suppress the protein abun-dance of MMP13 and restore the type II collagen release in IL-1 treated chondrocytes. Compared with single miRNA transfection, co-transfection of both miRNAs exceedingly abrogated the suppressed the protein production of TIMP1 caused by IL-1, thereby suggesting potent synergistic action. These results provided novel insights into the important function of miRNAs' collaboration in OA pathological development. The reduced MMP13, and enhanced TIMP1 protein production and type II collagen release also implies that miR-29a and miR-140 combination treatment may be a possible treatment for OA.
Xusheng Yang,Weijiu Huang,Xianghui Zhu,Ran Zhang,Fei Guo,Li Hu 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.11
The true stress–strain curves of an as-extruded state AA2099 were determined by uniaxial compression tests under varioushot deformation regimes. Constitutive analysis of the curves allowed calculation of the hot deformation activation energy(QHW). The microstructure of the as-compressed specimens were also characterized by electron back scatter diffraction(EBSD). EBSD data analyses showed that the major restoration mechanism corresponding to common industrial extrusionregimes was dynamic recovery. The precipitates in three states (T83, as-homogenized, and as-extruded) were characterizedby X-ray diffraction (XRD) phase analyses and transmission electron microscopy. The results revealed that the QHWwasclosely relevant to the precipitates strengthening. The finite element method simulation results and the microstructure of theextruded products confirmed that AA2099 in as-extruded state possessed good workability.
Influence of δ' Phase with GP‑I Zones Fillings on Slip Behavior and Cold Rolling Texture in AA2099
Xusheng Yang,Weijiu Huang,Xianghui Zhu,Fei Guo,Yanlong Ma,Linjiang Chai,Ran Zhang 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.9
The cold rolling texture of AA2099 sheets was strongly influenced by the prior heat treatment. Two typical heat treatments,including solid solution (T4) and underaging (T6) were utilized to control slip behavior by different precipitates during coldrolling. A much weaker texture with an orientation scatter towards higher Φ angles was found in the as-rolled T6 specimen(T6R). Slip behavior was analyzed using geometry necessary boundaries distribution. Results show that the T6R specimenexperienced a strong co-planar slip compared to the as-rolled T4 specimen. Prosperity of co-planar slips and limitation ofco-direction slips were main reasons for the weakening of Copper texture component in the T6R specimen. The distinguishslip behavior was caused by existence of δ' phase with GP-I zones fillings in the T6 specimen. This phase could be easilysheared by dislocations and then promoted co-planar slips in further deformation.
Junke Ou,Lin Yang,Xianghui Xi 대한금속·재료학회 2017 ELECTRONIC MATERIALS LETTERS Vol.13 No.1
Horn comb, an abundant biomass waste, has been successfully converted intoa hierarchical porous nitrogen doped carbon (HPNDC) via a simple and costeffectiveapproach. Tested as anode for sodium ion batteries (SIBs), horncomb derived carbon shows good rate capability and cycling stability,delivering a high initial charge capacity of 400 mAh g−1 at 100 mA g−1,retaining a reversible capacity of 112 mAh g−1 at 5 A g−1, and exhibiting acapacity of 241 mAh g−1 at 100 mA g−1 after 100 cycles. These superiorelectrochemical performances can be ascribed to its unique hierarchical porestructure combined with appropriate nitrogen doping effects. We believe thatour works will be helpful in promoting the development of high-rate and lowcostsodium ion batteries for large-scale energy storage systems.
Bacillus subtilis Spore Surface Display Technology: A Review of Its Development and Applications
( Guoyan Zhang ),( Yingfeng An ),( Hossain M. Zabed ),( Qi Guo ),( Miaomiao Yang ),( Jiao Yuan ),( Wen Li ),( Wenjin Sun ),( Xianghui Qi ) 한국미생물 · 생명공학회 2019 Journal of microbiology and biotechnology Vol.29 No.2
Bacillus subtilis spore surface display (BSSD) technology is considered to be one of the most promising approaches for expressing heterologous proteins with high activity and stability. Currently, this technology is used for various purposes, such as the production of enzymes, oral vaccines, drugs and multimeric proteins, and the control of environmental pollution. This paper presents an overview of the latest developments in BSSD technology and its application in protein engineering. Finally, the major limitations of this technology and future directions for its research are discussed.