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( Xing Jia Shen ),( Yong Zhu Yi ),( Shun Ming Tang ),( Zhi Fang Zhang ),( Yi Ren Li ),( Jia Lu He ),( Xiang Fu Wu ) 한국잠사학회 2007 International Journal of Industrial Entomology Vol.8 No.2
Bombyx mori nucleopolyhedrovirus(BmNPV) ecdysteroid UDP-glucosyltransferase gene (egt) promoter fragments of different lengths were amplified from BmNPV ZJ-8 genomic DNA by PCR. Reporter plasmids pBmegt542-luc, pBmegt309-luc and pBmegtl59-luc with luciferase (luc) driven by egt promoters were constructed. Both in vitro and in vivo expressions showed that BmNPV egt promoter activity requires the transactivation of viral factor(s), and expression of luc was detected earliest at 24 hrs post infection (pi). BmNPV ZJ-8 homologous region 3 (hr3) increased the expression of luc by over 1,600-fold. Molting hormone of 1.0∼2.0㎍/㎖ can dramatically down regulate expression of luc. Juvenile hormone analogue of 0.5∼2.0㎍/㎖ increased expression of luc by 145.8% to 75.7%. Deletion assay revealed that the promoter fragment of 159 bp contains the basal promoter structure; Promoter fragments of 309 bp and 542 bp showed similar but much higher transcriptional activities than that of 159 bp, suggesting that nucleotide from -159 to -309 nt upstream the translation initiation site harbors the main cis-acting elements.
Xiang-rong Zhang,Xiao-yan Chen,Lian-Dong Hu,Xing Tang,San-Ming Li,Da-fang Zhong 대한약학회 2005 Archives of Pharmacal Research Vol.28 No.8
The aim of this study was to compare two formulations of film-coated pellets containing clarithromycin after single oral dose study in healthy male volunteers. Two formulations with different coating polymers were prepared: formulation-1 (F-1) was prepared by incorporating three kinds of pH-dependent gradient-release coated pellets into capsules and formulation-2 (F-2) was prepared by coated with an insoluble semiosmotic film. Release profiles of filmcoated pellets were evaluated using paddle method under different conditions. Pharmacokinetic profiles of these formulations were obtained in three healthy male volunteers and compared to commercially available immediate release (IR) tablets. The relative bioavailability based on the AUC0-24 h was found to be 96.2% and 58.7% for F-1 and F-2 compared with IR, and the Tmax was delayed.
Xing-Jia Shen,Yong-Zhu Yi,Shun-Ming Tang,Zhi-Fang Zhang,Yi-Ren Li,Jia-Lu He,Xiang-Fu Wu 한국잠사학회 2004 International Journal of Industrial Entomology Vol.8 No.2
Bombyx mori nucleopolyhedrovirus (BmNPV) ecdysteroid UDP-glucosyltransferase gene (egt) promoter fragments of different lengths were amplified from BmNPV ZJ-8 genomic DNA by PCR. Reporter plasmids pBmegt542-luc, pBmegt309-luc and pBmegt159- luc with luciferase (luc) driven by egt promoters were constructed. Both in vitro and in vivo expressions showed that BmNPV egt promoter activity requires the transactivation of viral factor(s), and expression of luc was detected earliest at 24 hrs post infection (pi). BmNPV ZJ-8 homologous region 3 (hr3) increased the expression of luc by over 1,600-fold. Molting hormone of 1.0 ~ 2.0 mg/ml can dramatically down regulate expression of luc. Juvenile hormone analogue of 0.5 ~ 2.0 mg/ml increased expression of luc by 145.8% to 75.7%. Deletion assay revealed that the promoter fragment of 159 bp contains the basal promoter structure; Promoter fragments of 309 bp and 542 bp showed similar but much higher transcriptional activities than that of 159 bp, suggesting that nucleotide from -159 to -309 nt upstream the translation initiation site harbors the main cis-acting elements.
New multifunction materials with both electrorheological performance and luminescence property
Ming-Xing Chen,Yan-Li Shang,Yun-Ling Jia,Xiang-Yu Dong,Jing Ren,Jun-Ran Li 한국유변학회 2017 Korea-Australia rheology journal Vol.29 No.1
Novel multifunctional materials, the composites AlOOH-NaYFTb5 and AlOOH-NaYFTb10, containing AlO(OH) and β-NaYF4:5%Tb3+, have been synthesized via a facile hydrothermal route and a simple grinding method. The boehmite [AlO(OH)], yttrium nitrate [Y(NO3)3·6H2O], terbium nitrate, [Tb(NO3)3·6H2O], sodium citrate (Na3C6H5O7·2H2O) and sodium fluoride (NaF) were used as starting materials. The composition, electrorheological (ER) performance, and luminescence property of the functional materials were studied. Our results show that the composites not only have good electrorheological (ER) performance, but also have good optics property. The relative shear stress τr (τr = τE/τ0, τE and τ0 are the shear stresses at the electric field strength E = 4 and 0 kV/mm, respectively) values of the suspension (25 wt.%) of AlOOHNaYFTb5 material in silicone oil are all larger than 50 in a shear rate ranging from 0.06 to 26 s−1, the τr value reaches 1333 at a shear rate of 0.06 s−1. The material with such high ER activity and favorable luminescence performance is advantageous in its application as a multifunctional material.
Zhang Xiang-rong,Chen Xiao-yan,Hu Lian-Dong,Tang Xing,Li San-Ming,Zhong Da-fang The Pharmaceutical Society of Korea 2005 Archives of Pharmacal Research Vol.28 No.8
The aim of this study was to compare two formulations of film-coated pellets containing c1arithromycin after single oral dose study in healthy male volunteers. Two formulations with different coating polymers were prepared: formulation-1 (F-1) was prepared by incorporating three kinds of pH-dependent gradient-release coated pellets into capsules and formulation-2 (F-2) was prepared by coated with an insoluble semiosmotic film. Release profiles of filmcoated pellets were evaluated using paddle method under different conditions. Pharmacokinetic profiles of these formulations were obtained in three healthy male volunteers and compared to commercially available immediate release (IR) tablets. The relative bioavailability based on the $AUC_{0-24h}$ was found to be $96.2\%\;and\;58.7\%$ for F-1 and F-2 compared with IR, and the $T_{max}$ was delayed.
Yan Xing,Xiao-Hui Bai,Ming-Li Peng,Xiang-Rong Ma,Norbert Buske,Ya-Li Cui 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.10
Fe3O4/Au nanocomopsites (Fe3O4/Au NPs) with much improved catalytic activity were successfully fabricated through a simple seed growth method in aqueous solution. The petal-like structure, high saturation magnetization, the negatively charged sodium citrate-stabilized Fe3O4/Au NPs was characterized by transmission electron microscopy (TEM), X-ray diffractometer (XRD), dynamic light scattering (DLS) and vibrating sample magnetometry (VSM). The activated-H2O2 ability of Fe3O4/Au NPs was evaluated by using methylene blue (MB) as a cationic phenothiazines dye to be degraded in near neutral solution. The results showed Fe3O4/Au NPs removed over 95% MB from an aqueous solution within 60 min under the optimum conditions. The apparent rate constant of Fe3O4/Au NPs was 10.8 x 10 -2 min -1 which was 43.2 and 8.3 times higher than pure Fe3O4 (2.5 x 10 -3 min -1) and Au (1.3 x 10 -2 min -1) NPs. The enhanced catalytic activity and increased oxidation rate constant probably owing to the synergistic effect between Fe3O4 and Au NPs to activate H2O2 generate a large amount of strong oxidizing species, such as ·OH. In addition, nanocrystalline structure of Fe3O4/Au NPs was also very important to the peroxidase-like effect, especially the interaction interface between Fe3O4 and Au NPs. Moreover, Fe3O4/Au NPs was stable and could be regenerated and reused for at least five cycles.
Fibulin2: a negative regulator of BMSC osteogenic differentiation in infected bone fracture healing
Li Shi-Dan,Xing Wei,Wang Shao-Chuan,Li You-Bin,Jiang Hao,Zheng Han-Xuan,Li Xiao-Ming,Yang Jing,Guo De-Bin,Xie Xiao-Yu,Jiang Ren-Qing,Fan Chao,Li Lei,Xu Xiang,Fei Jun 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-
Bone fracture remains a common occurrence, with a population-weighted incidence of approximately 3.21 per 1000. In addition, approximately 2% to 50% of patients with skeletal fractures will develop an infection, one of the causes of disordered bone healing. Dysfunction of bone marrow mesenchymal stem cells (BMSCs) plays a key role in disordered bone repair. However, the specific mechanisms underlying BMSC dysfunction caused by bone infection are largely unknown. In this study, we discovered that Fibulin2 expression was upregulated in infected bone tissues and that BMSCs were the source of infection-induced Fibulin2. Importantly, Fibulin2 knockout accelerated mineralized bone formation during skeletal development and inhibited inflammatory bone resorption. We demonstrated that Fibulin2 suppressed BMSC osteogenic differentiation by binding to Notch2 and inactivating the Notch2 signaling pathway. Moreover, Fibulin2 knockdown restored Notch2 pathway activation and promoted BMSC osteogenesis; these outcomes were abolished by DAPT, a Notch inhibitor. Furthermore, transplanted Fibulin2 knockdown BMSCs displayed better bone repair potential in vivo. Altogether, Fibulin2 is a negative regulator of BMSC osteogenic differentiation that inhibits osteogenesis by inactivating the Notch2 signaling pathway in infected bone.
Zhang, Zhi-Fang,Shen, Xing-Jia,Yi, Yong-Zhu,Tang, Shun-Ming,Li, Yi-Ren,He, Jia-Lu,Wu, Xiang-Fu Korean Society of Sericultural Science 2004 International Journal of Industrial Entomology Vol.8 No.2
Bombyx mori nucleopolyhedrovirus(BmNPV) ecdysteroid UDP-glucosyltransferase gene (egt) promoter fragments of different lengths were amplified from BmNPV ZJ-8 genomic DNA by PCR. Reporter plasmids pBmegt542-luc, pBmegt309-luc and pBmegtl59-luc with luciferase (lue) driven by egt promoters were constructed. Both in vitro and in vivo expressions showed that BmNPV egt promoter activity requires the transactivation of viral factor(s), and expression of luc was detected earliest at 24 hrs post infection (pi). BmNPV ZJ-8 homologous region 3 (hr3) increased the expression of luc by over 1,600-fold. Molting hormone of 1.0 - 2.0 $\mu\textrm{g}$/$m\ell$ can dramatically down regulate expression of luc. Juvenile hormone analogue of 0.5-2.0 ${\mu}g$/$m\ell$ increased expression of luc by 145.8% to 75.7%. Deletion assay revealed that the promoter fragment of 159 bp contains the basal promoter structure; Promoter fragments of 309 bp and 542 bp showed similar but much higher transcriptional activities than that of 159 bp, suggesting that nucleotide from -159 to -309 nt upstream the translation initiation site harbors the main cis-acting elements.