A Computational Investigation of Fuel-Air Mixture Formation in the TR Combustion System

( He Xu ),( Xi Yan Gao ),( Wei Guo Liu ),( Chun Ming Liao ),( De Sheng Yang ) 한국액체미립화학회 2005 한국액체미립화학회 학술강연회 논문집 Vol.2005 No.-

A new combustion system applied in the 135-type diesel engine - TR(Three-Rapidity), was modified from deep cave ω combustion chamber with an oriented arc in the middle of chamber wall, and perfectly fit in a nozzle with orifices in conic section plus orifice in the center. In this paper, three-dimensional numerical simulation was completed and compared on TR combustion system and original 135 diesel engine combustion system. The results show that in TR combustion system, fuel ignition location is near TDC, and premix combustion proportion increases. The oriented arc, contracted cave and centric spray contribute to enhancing air flow, making more reasonable fuel distribution and formatting more homogeneous mixture. The usage of air in chamber is effectively increased and heat loss from the chamber wall is obviously decreased. The cold start performance of the engine is improved also. A performance test on TR combustion system was made on a 135-type diesel engine. The results demonstrate that TR has better exhaust smoke than original engine. At the low and part load, NOX emission of TR combustion system is less than original diesel, but more at high load conditions, which could be solved by delaying fuel spray timing. TR combustion has much potential to improve emission with less structure changed of original engine, so it has large foreground.

Hypoxia-Inducible Factor 1 Promoter-Induced JAB1 Overexpression Enhances Chemotherapeutic Sensitivity of Lung Cancer Cell Line A549 in an Anoxic Environment

Hu, Ming-Dong,Xu, Jian-Cheng,Fan, Ye,Xie, Qi-Chao,Li, Qi,Zhou, Chang-Xi,Mao, Mei,Yang, Yu Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

The presence of lung cancer cells in anoxic zones is a key cause od chemotherapeutic resistance. Thus, it is necessary to enhance the sensitivity of such lung cancer cells. However, loss of efficient gene therapeutic targeting and inefficient objective gene expression in the anoxic zone in lung cancer are dilemmas. In the present study, a eukaryotic expression plasmid pUC57-HRE-JAB1 driven by a hypoxia response elements promoter was constructed and introduced into lung cancer cell line A549. The cells were then exposed to a chemotherapeutic drug cis-diamminedichloroplatinum (C-DDP). qRT-PCR and western blotting were used to determine the mRNA and protein level and flow cytometry to examine the cell cycle and apoptosis of A549 transfected pUC57-HRE-JAB1. The results showed that JAB1 gene in the A549 was overexpressed after the transfection, cell proliferation being arrested in G1 phase and the apoptosis ratio significantly increased. Importantly, introduction of pUC57-HRE-JAB1 significantly increased the chemotherapeutic sensitivity of A549 in an anoxic environment. In conclusion, JAB1 overexpression might provide a novel strategy to overcome chemotherapeutic resistance in lung cancer.

Molecular and Biochemical Characterization of a Novel Xylanase from Massilia sp. RBM26 Isolated from the Feces of Rhinopithecus bieti

( Bo Xu ),( Li Ming Dai ),( Jun Jun Li ),( Meng Deng ),( Hua Biao Miao ),( Jun Pei Zhou ),( Yue Lin Mu ),( Qian Wu ),( Xiang Hua Tang ),( Yun Juan Yang ),( Jun Mei Ding ),( Nan Yu Han ),( Zun Xi Huang 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.1

Xylanases sourced from different bacteria have significantly different enzymatic properties. Therefore, studying xylanases from different bacteria is important to their applications in different fields. A potential xylanase degradation gene in Massilia was recently discovered through genomic sequencing. However, its xylanase activity remains unexplored. This paper is the first to report a xylanase (XynRBM26) belonging to the glycosyl hydrolase family (GH10) from the genus Massilia. The gene encodes a 383-residue polypeptide (XynRBM26) with the highest identity of 62% with the endoxylanase from uncultured bacterium BLR13. The XynRBM26 expressed in Escherichia coli BL21 is a monomer with a molecular mass of 45.0 kDa. According to enzymatic characteristic analysis, pH 5.5 is the most appropriate for XynRBM26, which could maintain more than 90% activity between pH 5.0 and 8.0. Moreover, XynRBM26 is stable at 37°C and could maintain at least 96% activity after being placed at 37°C for 1 h. This paper is the first to report that GH10 xylanase in an animal gastrointestinal tract (GIT) has salt tolerance, which could maintain 86% activity in 5 M NaCl. Under the optimum conditions, Km, Vmax, and kcat of XynRBM26 to beechwood xylan are 9.49 mg/ml, 65.79 μmol/min/mg, and 47.34 /sec, respectively. Considering that XynRBM26 comes from an animal GIT, this xylanase has potential application in feedstuff. Moreover, XynRBM26 is applicable to high-salt food and seafood processing, as well as other high-salt environmental biotechnological fields, because of its high catalytic activity in high-concentration NaCl.

Adjuvant Radiotherapy after Breast Conserving Treatment for Breast Cancer:A Dosimetric Comparison between Volumetric Modulated Arc Therapy and Intensity Modulated Radiotherapy

Liu, Zhe-Ming,Ge, Xiao-Lin,Chen, Jia-Yan,Wang, Pei-Pei,Zhang, Chi,Yang, Xi,Zhu, Hong-Cheng,Liu, Jia,Qin, Qin,Xu, Li-Ping,Lu, Jing,Zhan, Liang-Liang,Cheng, Hong-Yan,Sun, Xin-Chen Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.8

Background: Radiotherapy is an important treatment of choice for breast cancer patients after breast-conserving surgery, and we compare the feasibility of using dual arc volumetric modulated arc therapy (VMAT2), single arc volumetric modulated arc therapy (VMAT1) and Multi-beam Intensity Modulated Radiotherapy (M-IMRT) on patients after breast-conserving surgery. Materials and Methods: Thirty patients with breast cancer (half right-sided and half left-sided) treated by conservative lumpectomy and requiring whole breast radiotherapy with tumor bed boost were planned with three different radiotherapy techniques: 1) VMAT1; 2) VMAT2; 3) M-IMRT. The distributions for the planning target volume (PTV) and organs at risk (OARs) were compared. Dosimetries for all the techniques were compared. Results: All three techniques satisfied the dose constraint well. VMAT2 showed no obvious difference in the homogeneity index (HI) and conformity index (CI) of the PTV with respect to M-IMRT and VMAT1. VMAT2 clearly improved the treatment efficiency and can also decrease the mean dose and V5Gy of the contralateral lung. The mean dose and maximum dose of the spinal cord and contralateral breast were lower for VMAT2 than the other two techniques. The very low dose distribution (V1Gy) of the contralateral breast also showed great reduction in VMAT2 compared with the other two techniques. For the ipsilateral lung of right-sided breast cancer, the mean dose was decreased significantly in VMAT2 compared with VMAT1 and M-IMRT. The V20Gy and V30Gy of the ipsilateral lung of the left-sided breast cancer for VMAT2 showed obvious reduction compared with the other two techniques. The heart statistics of VMAT2 also decreased considerably compared to VMAT1 and M-IMRT. Conclusions: Compared to the other two techniques, the dual arc volumetric modulated arc therapy technique reduced radiation dose exposure to the organs at risk and maintained a reasonable target dose distribution.

Novel and Effective Almagate Enema for Hemorrhagic Chronic Radiation Proctitis and Risk Factors for Fistula Development

Yuan, Zi-Xu,Ma, Teng-Hui,Zhong, Qing-Hua,Wang, Huai-Ming,Yu, Xi-Hu,Qin, Qi-Yuan,Chu, Li-Li,Wang, Lei,Wang, Jian-Ping Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.2

Radiation proctitis is a common complication after radiotherapy for pelvic malignant tumors. This study was conducted to assess the efficacy of novel almagate enemas in hemorrhagic chronic radiation proctitis (CRP) and evaluate risk factors related to rectal deep ulcer or fistula secondary to CRP. All patients underwent a colonoscopy to confirm the diagnosis of CRP and symptoms were graded. Typical endoscopic and pathological images, risk factors, and quality of life were also recorded. A total of 59 patients were enrolled. Gynecological cancers composed 93.1% of the primary malignancies. Complete or obvious reduction of bleeding was observed in 90% (53/59) patients after almagate enema. The mean score of bleeding improved from 2.17 to 0.83 (P<0.001) after the enemas. The mean response time was 12 days. No adverse effects were found. Moreover, long-term successful rate in controlling bleeding was 69% and the quality of life was dramatically improved (P=0.001). The efficacy was equivalent to rectal sucralfate, but the almagate with its antacid properties acted more rapidly than sucralfate. Furthermore, we firstly found that moderate to severe anemia was the risk factor of CRP patients who developed rectal deep ulcer or fistulas (P= 0.015). We also found abnormal hyaline-like thick wall vessels, which revealed endarteritis obliterans and the fibrosis underlying this disease. These findings indicate that almagate enema is a novel effective, rapid and well-tolerated method for hemorrhagic CRP. Moderate to severe anemia is a risk factor for deep ulceration or fistula.

Study on Grinding Characteristics of Different Crystal Surfaces of Sapphire

Yi-Qing Yu,Ming-Jian Shao,Chang-Cai Cui,Zhong-Wei Hu,Xi-Peng Xu 한국생산제조학회 2016 한국생산제조시스템학회 학술발표대회 논문집 Vol.2016 No.10

Apoptosis of Human Burkitt’s Lymphoma Cells Induced by 2-N,NDiethylaminocarbonyloxymethyl-1-diphenylmethyl-4-(3,4,5-trimethoxybenzoyl) piperazine Hydrochloride (PMS-1077)

Wen-di Wang,Xi-ming Xu,Ying Chen,Peng Jiang,Chang-zhi Dong,Qin Wang 대한약학회 2009 Archives of Pharmacal Research Vol.32 No.12

Piperazine is one of the heterocycles which are associated with diverse pharmacological activities. 2-N,N-Diethylaminocarbonyloxymethyl-1-diphenylmethyl-4-(3,4,5-trimethoxybenzoyl) piperazine hydrochloride (PMS-1077) is a trisubstituted piperazine which contains a trimethoxybenzene ring and a benzhydrylpiperazine fragment, both of which can induce cell proliferation regression by different mechanisms. We have therefore examined the effects of PMS-1077 on Human Burkitt’s lymphoma cells (Raji). The viability of Raji cells was determined by MTT assay and also assessed by trypan blue dye exclusion method. The results demonstrate that PMS-1077 can suppress the proliferation of Raji cells in a dose- and timedependent manner, while inhibit colony formation ability of Raji cells merely in a dose-dependent manner in vitro. Meanwhile, morphological changes were observed using fluorescence microscope. Flow cytometric analysis through PI stains showed that PMS-1077 blocked the growth of Raji cells in the G0/G1 period, and induced apoptosis of Raji cells after 48 h of incubation. Cell apoptosis induced by PMS-1077 was further confirmed by staining with Annexin-V FITC and PI. Preliminary study by molecular docking suggests that PMS-1077 may inhibit tubulin polymerization. More experiments are in progress in our laboratory to reveal the mode of action of PMS-1077 in the induction of apoptosis of Raji cells.

Investigation on Texture, Magnetic Properties and Inhomogeneities of Hot Deformed Nd–Fe–B Magnet

Hongjian Li,Qiong Wu,Ming Yue,Xiaochang Xu,Yuqing Li,Jingming Liang,Wang Xi,Jiuxing Zhang 한국자기학회 2018 Journal of Magnetics Vol.23 No.3

The anisotropic hot deformed nanocyrstalline Nd.Fe.B magnet was prepared by spark plasma sintering technique using MQU-F powders. The location dependence along radial direction on the magnetic properties, microstructure and crystal alignment of the hot deformed magnet have been investigated. The inhomogeneity of the magnetic performance along radial direction was revealed with a vibrating sample magnetometer (VSM). The crystal structure and microstructure were characterized by X-ray diffractometry (XRD) and scanning electron microscopy (SEM), respectively. In addition, quantitative texture analysis was carried out based on the electron backscattered diffraction technique (EBSD). The experimental results reveal that the coordination of grain size and orientation of Nd.Fe.B grains leads to the inhomogeneities of magnetic performance.

Effect of Choice Feeding on Performance, Gastrointestinal Development and Feed Utilization of Broilers

Yao, Junhu,Tian, Xiaoyan,Xi, Haibo,Han, Jincheng,Xu, Ming,Wu, Xiaobing Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.1

The experiment was conducted to study the effect of choice feeding on growth performance, carcass quality, gastrointestinal development and feed utilization of 22-49 days old broilers. One hundred and forty four 22-day-old broilers were randomly allocated to 3 treatments with 4 replicates per treatment and 12 birds per replicate. Three feeding regimes are complete diet (control), ground corn and protein concentrate (treatment I), and soybean meal and balancer (treatment II). Protein concentrate is the residue part of complete diet without corn, and balancer is the residue part of complete diet without soybean meal. Treatment I and II are designed for the broilers to freely choose the two parts of diet. The results showed that: (1) broilers under choice feeding (treatment I and II) had lower performances compared with the control; (2) gastrointestinal development and the efficiency ratios that broilers converted dietary crude protein and lysine to body weight gain were improved in treatment I (p<0.05); (3) there were no significant differences in the apparent metabolizabilities of dietary dry matter, crude protein and gross energy, and deposition ratios of dietary nitrogen and energy, and carcass quality among three feeding regimes (p>0.05).

Complete mitochondrial genome of the meadow moth, Loxostege sticticalis (Lepidoptera: Pyraloidea: Crambidae), compared to other Pyraloidea moths

Hong-Fang Ma,Xi-Xi Zheng,Ming-Hui Peng,Hai-Xu Bian,Miao-Miao Chen,Yan-Qun Liu,Xing-Fu Jiang,Li Qin 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.3

The mitochondrial genome (mitogenome) is an important topic for comparative and evolutionary genomics, as well as phylogenetic and population genetics. However, there are limited data regarding the mitochondrial genome available of Pyraloidea, one of the largest superfamilies in Lepidoptera. In this report, we present the complete mitogenome of the meadow moth, Loxostege sticticalis L. (Lepidoptera: Pyraloidea: Crambidae), which is a serious economic pest of both crops and weedsworldwide, thereby enhancing the available genomic information for Pyraloidea. This circular genome is 15,218 bp in length, containing 13 protein-coding genes (PCGs), two rRNA genes (rRNAs), and 22 tRNA genes (tRNAs), with a typical gene orientation and order comparable to other sequenced Pyraloidea insects. The genome composition of the major strand exhibits highly AT bias (80.82%), with a slightly positive AT skew indicating the occurrence of more As than Ts. The L. sticticalis mitogenome has a total of 130 bp of intergenic spacer sequences spread over 15 regions, ranging in size from 1 to 48 bp, of which only two are common among the 23 total Pyraloidea moths that have data collected on the mitogenome (one is located between tRNAGln and ND2 with variation change in length and a limited sequence conservation, and the other is located between tRNASer(UCN) and ND1 with a conserved 6 bp motif ‘ATACTA’). The A + Trich region of 331 bp in the genome is comprised of non-repetitive sequences but contains an ATAGN motif followed by a poly-T stretch of 17 bp, a microsatellite-like (TA)11 element preceded by an ATTTA motif, and a poly-A stretch upstream tRNAMet. These conserved structures identified in the A + T-rich region are presented in all of the sequenced Pyraloidea species. We provide a mitogenome-based phylogeny of Pyraloidea species, in which L. sticticalis shares close ancestry to Ostrinia species with substantial evidence. Our phylogenetic analyses strongly divide Crambidae into two sister lineages, one consisting of Pyraustinae and Spilomelinae, while the other contains Crambinae, Acentropinae, Scopariinae, Schoenobiinae and Glaphyriinae. The mitogenome dataset also supports the basal split between Pyraustinae and Spilomelinae.