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徐哲俊(Xu,Zhe-Jun),金红,英(Xu,Zhe-Jun),金春洙(Kim, Chun-Soo) 글로벌경영학회 2012 글로벌경영학회지 Vol.9 No.3
With the development of Yanbian tourism and entering from all directions of foreign enterprises, which will have a great impact on the development and management of tourism human resources in China. As Yanbian tourism develops rapidly in recent years, the demand for tourism human resources has a rapid expansion. However, the development and management of human resources is relatively backward, which has seriously limited the development of Yanbian tourism and needs to be solved as soon as possible. Therefore, this paper elaborates the human resources of Yanbian, analyses the problems about the development and management of human resources, and then finds that the total quantity of Yanbian human resources is insufficient, whose personnel training has a great gap compared with its market demand;its quality and level as a whole is somewhat low; professionals of tourism and hotel management are deficient;training work falls behind, which can not satisfy the tourism demand;incentive mechanism is on shortage, which leads to a frequent flow of personnel;employees have a weak sense of service. Then, it constructed a “Trinity” pattern of human resources development and management in Yanbian tourism. This paper puts forward practical countermeasure and measures from 6 aspects of establishing the correct concept of tourism talents, letting local governments and tourism administration departments play a leading role sufficiently, improving and strengthening the tourism human resources training, improving Yanbian tourism education system, building perfect talent and incentive mechanism, strengthening the occupation career management of tourism enterprise employee and so on, which provides theoretic and practical reference for the scientific development of human resources in Yanbian. 旅游业的持续快速发展以及国外旅游企业的全方位进入,将对中国旅游人力资源开发与管理 带来极大的冲击。近年来延边旅游业迅猛发展,旅游业对人力资源的需求急剧膨胀,但是,旅 游人力资源的开发与管理的相对滞后,严重制约着延边旅游业的发展,需要迅速加以解决。因 此,本文首先阐述了延边旅游人力资源的现状,分析了旅游人力资源开发与管理中存在的问 题,发现了延边旅游人力资源总量相对不足,人才培养滞后于市场需求;整体素质及层次偏 低;旅游及酒店管理专业人才匮乏;培训工作落后,不能满足旅游业的需求;缺乏有效的激励 机制,人员流动频繁;从业人员服务意识薄弱。然后,构建了延边旅游业“三位一体”人力资源开 发与管理模式。最后,提出了树立正确的旅游人才观念、充分发挥地方政府和旅游行政管理部 门的指导作用、完善和加强旅游人力资源培训、改善延边旅游教育体系、建立完善的人才使用 和激励机制、强化旅游企业员工职业生涯管理等6个方面提出了切实可行的对策和措施,为延边 旅游企业人力资源的科学发展提供理论与实践。鉴。
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Jun Ki Son,Nguyen Xu Do,박정규 한국응용곤충학회 2008 Journal of Asia-Pacific Entomology Vol.11 No.4
Seasonal parasitism of adult Riptortus clavatus (Thunberg) (Heteroptera: Alydidae) by the tachinid fly parasitoid, Dionaea magnifrons (Herting), was monitored at three sites in Korea, 2006 by using the aggregation pheromone traps of R. clavatus. Basic developmental characteristics of the parasitoid were also examined. Annual parasitism of R. clavatus female and male adults was 6.8 and 11.6%, respectively. Parasitism of females and males of the over-wintering (Oct. to Jun.) generation (10.6 and 16.5%, respectively) was higher than the summer (Jul. to Sep.) generation (2.1 and 2.5%). Maximum parasitism for males was recorded during spring or autumn, reaching 35.6, 25.0, and 26.9% at the university campus, and pear and sweet persimmon orchards, respectively. Sex ratio of all the D. magnifrons was slightly male-dominated (62.2%). The pupal period of D. magnifrons was 9.2 days for both sexes in room temperature conditions. D. magnifrons larvae escaped from R. clavatus in 8.0±3.4 days after host bug collection fromthe field during April to September, whereas in October and November they emerged in 15.1±3.8 days. This parasitoid fly should be studied further to determine its effectiveness as a biological control agent complementary to existing strategies in the management of bean bug. Seasonal parasitism of adult Riptortus clavatus (Thunberg) (Heteroptera: Alydidae) by the tachinid fly parasitoid, Dionaea magnifrons (Herting), was monitored at three sites in Korea, 2006 by using the aggregation pheromone traps of R. clavatus. Basic developmental characteristics of the parasitoid were also examined. Annual parasitism of R. clavatus female and male adults was 6.8 and 11.6%, respectively. Parasitism of females and males of the over-wintering (Oct. to Jun.) generation (10.6 and 16.5%, respectively) was higher than the summer (Jul. to Sep.) generation (2.1 and 2.5%). Maximum parasitism for males was recorded during spring or autumn, reaching 35.6, 25.0, and 26.9% at the university campus, and pear and sweet persimmon orchards, respectively. Sex ratio of all the D. magnifrons was slightly male-dominated (62.2%). The pupal period of D. magnifrons was 9.2 days for both sexes in room temperature conditions. D. magnifrons larvae escaped from R. clavatus in 8.0±3.4 days after host bug collection fromthe field during April to September, whereas in October and November they emerged in 15.1±3.8 days. This parasitoid fly should be studied further to determine its effectiveness as a biological control agent complementary to existing strategies in the management of bean bug.
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( Jun Mei Ding ),( Ting Ting Yu ),( Lian Ming Liang ),( Zhen Rong Xie ),( Yun Juan Yang ),( Jun Pei Zhou ),( Bo Xu ),( Jun Jun Li ),( Zun Xi Huang ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.11
The esterase gene Est8 from the thermophilic bacterium Bacillus sp. K91 was cloned and expressed in Escherichia coli. The monomeric enzyme exhibited a theoretical molecular mass of 24.5 kDa and an optimal activity around 50°C at pH 9.0. A model of Est8 was constructed using a hypothetical YxiM precursor structure (2O14_A) from Bacillus subtilis as template. The structure showed an α/β-hydrolase fold and indicated the presence of a typical catalytic triad consisting of Ser-11, Asp-182, and His-185, which were investigated by site directed replacements coupled with kinetic characterization. Asp-182 and His-185 residues were more critical than the Ser-11 residue in the catalytic activity of Est8. A comparison of the amino acid sequence showed that Est8 could be grouped into the GDSL family and further classified as an SGNH hydrolase. Est8 is a new member of the SGNH hydrolase subfamily and may employ a different catalytic mechanism.
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Lu Xu,Chun-Qing Zhao,De-Jin Xu,Guang-Chun Xu,Xiao-Long Xu,Zhao-Jun Han,Ya-Nan Zhang,Zhong-Yan Gu 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.2
Nuclear receptors (NRs), which belong to a superfamily of ligand-dependent transcription factors, play essential roles in gene regulation to affect numerous physiological pathways. Twenty NR genes were identified in Nilaparvata lugens by using genomic, transcriptomic and GenBank databases, and categorized into NR0-NR6 subfamilies according to standard nomenclature. Among them, three NR geneswere classed into NR0, four into NR1, eight into NR2, one into NR3, one into NR4, two into NR5, and one into NR6, respectively. A phylogenetic tree of NRs from N. lugens and other representative species was constructed, which provided evolutionary insight into genetic distance. In order to investigate the NRs,whichwere induced by sulfoxaflor, time- and tissue-specific expression profiles of NR genes in fourth-instar nymphs were determined following LD50 sulfoxaflor treatment (0.28 ng/insect) and compared with control samples. NlUSP, NlE78, NlTLL, NlHR51, NlHR83, NlPNR and NlFTZ-F1 were highly expressed following 12–48 h of sulfoxaflor treatment. NlHR39 and NlDSF transcripts were detected in the head and the thorax, respectively. High-level and constitutive expression of NlHR3, NlTLL, NlHR83, NlFTZF1 and NlHR4 were found in the abdomen. Feeding of dsRNAs reduced the expression of NlHR3, NlUSP, NlTLL, NlHR83, NlPNR, NlFTZ-F1 and NlHR4 (35.48–49.77%) and caused significant nymph mortality (69.21–81.45%). These NRs, considered as insecticide targets, may play important function in sulfoxaflor detoxification.
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( Bo Xu ),( Fu Ya Yang ),( Cai Yun Xiong ),( Jun Jun Li ),( Xiang Hua Tang ),( Jun Pei Zhou ),( Zhen Rong Xie ),( Jun Mei Ding ),( Yun Juan Yang ),( Zun Xi Huang ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.4
To isolate novel and useful microbial enzymes from uncultured gastrointestinal microorganisms, a fecal microbial metagenomic library of the pygmy loris was constructed. The library was screened for amylolytic activity, and 8 of 50,000 recombinant clones showed amylolytic activity. Subcloning and sequence analysis of a positive clone led to the identification a novel gene (amyPL) coding for α-amylase. AmyPL was expressed in Escherichia coli BL21 (DE3) and the purified AmyPL was enzymatically characterized. This study is the first to report the molecular and biochemical characterization of a novel α-amylase from a gastrointestinal metagenomic library.
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( Bo Xu ),( Li Ming Dai ),( Jun Jun Li ),( Meng Deng ),( Hua Biao Miao ),( Jun Pei Zhou ),( Yue Lin Mu ),( Qian Wu ),( Xiang Hua Tang ),( Yun Juan Yang ),( Jun Mei Ding ),( Nan Yu Han ),( Zun Xi Huang 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.1
Xylanases sourced from different bacteria have significantly different enzymatic properties. Therefore, studying xylanases from different bacteria is important to their applications in different fields. A potential xylanase degradation gene in Massilia was recently discovered through genomic sequencing. However, its xylanase activity remains unexplored. This paper is the first to report a xylanase (XynRBM26) belonging to the glycosyl hydrolase family (GH10) from the genus Massilia. The gene encodes a 383-residue polypeptide (XynRBM26) with the highest identity of 62% with the endoxylanase from uncultured bacterium BLR13. The XynRBM26 expressed in Escherichia coli BL21 is a monomer with a molecular mass of 45.0 kDa. According to enzymatic characteristic analysis, pH 5.5 is the most appropriate for XynRBM26, which could maintain more than 90% activity between pH 5.0 and 8.0. Moreover, XynRBM26 is stable at 37°C and could maintain at least 96% activity after being placed at 37°C for 1 h. This paper is the first to report that GH10 xylanase in an animal gastrointestinal tract (GIT) has salt tolerance, which could maintain 86% activity in 5 M NaCl. Under the optimum conditions, Km, Vmax, and kcat of XynRBM26 to beechwood xylan are 9.49 mg/ml, 65.79 μmol/min/mg, and 47.34 /sec, respectively. Considering that XynRBM26 comes from an animal GIT, this xylanase has potential application in feedstuff. Moreover, XynRBM26 is applicable to high-salt food and seafood processing, as well as other high-salt environmental biotechnological fields, because of its high catalytic activity in high-concentration NaCl.
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Preparation of lithium-doped NaV6O15 thin film cathodes with high cycling performance in SIBs
Xu Hai-Yan,Ruan Jun Hai,Liu Fang Lin,Li Dong-Cai,Zhang Feng-Jun,Wang Ai-Guo,Sun Dao-Sheng,오원춘 한국세라믹학회 2022 한국세라믹학회지 Vol.59 No.3
Lithium ions-doped NaV6O15 thin films have been prepared using a simple low temperature liquid phase deposition method and subsequent annealing process. X-ray diffraction (XRD), Fourier transform infrared spectrometer (FTIR), scanning elec- tron microscopy (SEM), and photoelectron spectroscopy (XPS) have been used to study the structural and physicochemical characteristics of the NaV6O15 film. The films were grown on the FTO conductive glass and used directly as an electrode of sodium ion batteries. The prepared lithium ions-doped NaV6O15 thin film electrodes showed an excellent cycling stability and discharge capacity, which may be attributed to the stability of the Li+ embedded into the gap between the V–O layers to maintain the structure and its stable β-phase structure transformed after the first cycle. The cycling stability greatly improved with increasing annealing temperature, while the discharge capacity decreased. The capacities of the film electrodes annealed at 400 °C and 450 °C maintained above 97% after 100 cycles. The lithium-doped NaV6O15 underwent a phase transition dur- ing the first charge/discharge cycle. The new transformed phase has perfect crystal structure stability undergoing insertion and deinsertion of Na+. Therefore, the lithium-doped NaV6O15 thin film possesses good cycling stability and is expected to be a promising thin film cathode for sodium-ion batteries.
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Xu, Jun,Dong, Fengshou,Liu, Xingang,Li, Jing,Li, Yuanbo,Shan, Weili,Zheng, Yongquan Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.12
A method based on ethyl acetate extraction and gas chromatography with tandem mass spectrometry was developed for determining tetraconazole residues in fruits and vegetables. A 10 g homogenized sample was mixed with 10 mL ethyl acetate, shaken vigorously for 3 min, stored at $-20^{\circ}C$ for 15 min, and then vortexed vigorously for 1 min; 1 g NaCl and 4 g anhydrous $MgSO_4$ were added. The clean-up was carried out by applying dispersive solid-phase with 150 mg $MgSO_4$and 50 mg primary secondary amine. Three precursor product ion transitions for tetraconazole were measured and evaluated to provide the maximum degree of confidence. Average recoveries in fruits and vegetables at three levels (0.005, 0.05 and 0.5 mg/kg) ranged from 85.53% to 110.66% with relative standard deviations ($RSD_r$) from 1.3% to 17.5%. The LODs ranged from 0.002 to 0.004 ${\mu}g$/kg, and LOQs ranged from 0.006 to 0.012 ${\mu}g$/kg. This method was also applied to determine tetraconazole residue in cucumber dissipation experiment under field conditions. The half-lives of tetraconazole in cucumber were in the range of 2.1-3.1 days.
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