Physciosporin suppresses the proliferation, motility and tumourigenesis of colorectal cancer cells

Ta&#x15f,, &#x130,sa,Han, Jin,Park, So-Yeon,Yang, Yi,Zhou, Rui,Gamage, Chathurika D.B.,Van Nguyen, Tru,Lee, Ji-Yoon,Choi, Yong Jae,Yu, Young Hyun,Moon, Kyung-Sub,Kim, Kyung Keun,Ha, Hyung-Ho,Kim, Sang Elsevier 2019 Phytomedicine Vol.56 No.-

<P><B>Abstract</B></P> <P><B>Background</B></P> <P>Lichens, which represent symbiotic associations of fungi and algae, are potential sources of numerous natural products. Physciosporin (PHY) is a potent secondary metabolite found in lichens and was recently reported to inhibit the motility of lung cancer cells via novel mechanisms.</P> <P><B>Purpose</B></P> <P>The present study investigated the anticancer potential of PHY on colorectal cancer (CRC) cells.</P> <P><B>Methods</B></P> <P>PHY was isolated from lichen extract by preparative TLC. The effect of PHY on cell viability, motility and tumourigenicity was elucidated by MTT assay, hoechst staining, flow cytometric analysis, transwell invasion and migration assay, soft agar colony formation assay, Western blotting, qRT-PCR and PCR array <I>in vitro</I> as well as tumorigenicity study <I>in vivo</I>.</P> <P><B>Results</B></P> <P>PHY decreased the viability of various CRC cell lines (Caco2, CT26, DLD1, HCT116 and SW620). Moreover, PHY elicited cytotoxic effects by inducing apoptosis at toxic concentrations. At non-toxic concentrations, PHY dose-dependently suppressed the invasion, migration and colony formation of CRC cells. PHY inhibited the motility of CRC cells by suppressing epithelial-mesenchymal transition and downregulating actin-based motility markers. In addition, PHY downregulated β-catenin and its downstream target genes cyclin-D1 and c-Myc. Moreover, PHY modulated KAI1 C-terminal-interacting tetraspanin and KAI1 expression, and downregulated the downstream transcription factors c-jun and c-fos. Finally, PHY administration showed considerable bioavailability and effectively decreased the growth of CRC xenografts in mice without causing toxicity.</P> <P><B>Conclusion</B></P> <P>PHY suppresses the growth and motility of CRC cells via novel mechanisms.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

XRD studies on phase formation and the crystallite structure of BaTiO3 synthesized by HBM: the effect of calcination temperature

X.M. Chen,Y. Zhang,W.W. Kong,X.B. Bian,J.P. Zhou,P. Liu 한양대학교 세라믹연구소 2010 Journal of Ceramic Processing Research Vol.11 No.4

Barium titanate (BaTiO3, or BT) powders were synthesized via a solid state reaction of BaCO3-TiO2 by combining a highenergy ball milling (HBM) technique and a calcination method. Using X-ray diffraction (XRD) methods with a Rietveld refinement, the effects of calcination temperature on the phase formation and crystallite structure of BT powders were studied. It is found that by means of HBM the synthesis temperature for obtaining BT single phase is decreased to 960 oC, which is much lower than that required by the conventional solid-state reaction process, and the crystallite structure of BT is in the (pseudo)cubic form. As the calcination temperature is increased to 980 oC, the structure of BT crystallites transforms from the (pseudo)cubic to the tetragonal form. With an increase in the calcination temperature, both the tetragonality (c/a-1) and crystallite size are increased.

Alluaudite Na₂Co₂Fe(PO₄)₃ as an electroactive material for sodium ion batteries

Essehli, R.,Belharouak, I.,Ben Yahia, H.,Maher, K.,Abouimrane, A.,Orayech, B.,Calder, S.,Zhou, X. L.,Zhou, Z.,Sun, Y-K. The Royal Society of Chemistry 2015 Dalton Transactions Vol.44 No.17

<P>The electroactive orthophosphate Na<SUB>2</SUB>Co<SUB>2</SUB>Fe(PO<SUB>4</SUB>)<SUB>3</SUB> was synthesized using a solid state reaction. Its crystal structure was solved using the combination of powder X-ray- and neutron-diffraction data. This material crystallizes according to the alluaudite structure (S.G. <I>C</I>2/<I>c</I>). The structure consists of edge sharing [MO<SUB>6</SUB>] octahedra (M = Fe, Co) resulting in chains parallel to [−101]. These chains are linked together <I>via</I> the [PO<SUB>4</SUB>] tetrahedra to form two distinct tunnels in which sodium cations are located. The electrochemical properties of Na<SUB>2</SUB>Co<SUB>2</SUB>Fe(PO<SUB>4</SUB>)<SUB>3</SUB> were evaluated by galvanostatic charge–discharge cycling. During the first discharge to 0.03 V, Na<SUB>2</SUB>Co<SUB>2</SUB>Fe(PO<SUB>4</SUB>)<SUB>3</SUB> delivers a specific capacity of 604 mA h g<SUP>−1</SUP>. This capacity is equivalent to the reaction of more than seven sodium ions per formula unit. Hence, this is a strong indication of a conversion-type reaction with the formation of metallic Fe and Co. The subsequent charge and discharge involved the reaction of fewer Na ions as expected for a conversion reaction. When discharged to 0.9 V, the material intercalated only one Na<SUP>+</SUP>-ion leading to the formation of a new phase Na<SUB>3</SUB>Co<SUB>2</SUB>Fe(PO<SUB>4</SUB>)<SUB>3</SUB>. This phase could then be cycled reversibly with an average voltage of 3.6 V <I>vs.</I> Na<SUP>+</SUP>/Na and a capacity of 110 mA h g<SUP>−1</SUP>. This result is in good agreement with the theoretical capacity expected from the extraction/insertion of two sodium atoms in Na<SUB>3</SUB>Co<SUB>2</SUB>Fe(PO<SUB>4</SUB>)<SUB>3</SUB>.</P> <P>Graphic Abstract</P><P>Na<SUB>2</SUB>Co<SUB>2</SUB>Fe(PO<SUB>4</SUB>)<SUB>3</SUB> crystallizes with the alluaudite-type structure (S.G. <I>C</I>2/<I>c</I>) and plays a dual anode/cathode behavior in sodium ion batteries. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c5dt00971e'> </P>

Improvement of lower hybrid current drive systems for high-power and long-pulse operation on EAST

Wang M.,Liu L.,Zhao L.M.,Li M.H.,Ma W.D.,Hu H.C.,Wu Z.G.,Feng J.Q.,Yang Y.,Zhu L.,Chen M.,Zhou T.A.,Jia H.,Zhang J.,Cao L.,Zhang L.,Liang R.R.,Ding B.J.,Zhang X.J.,Shan J.F.,Liu F.K.,Ekedahl A.,Gonich 한국원자력학회 2022 Nuclear Engineering and Technology Vol.54 No.11

Aiming at high-power and long-pulse operation up to 1000 s, some improvements have been made for both 2.45 GHz and 4.6 GHz lower hybrid (LH) systems during the recent 5 years. At first, the guard limiters of the LH antennas with graphite tiles were upgraded to tungsten, the most promising material for plasma facing components in nuclear fusion devices. These new guard limiters can operate at a peak power density of 12.9 MW/m2 . Strong hot spots were usually observed on the old graphite limiters when 4.6 GHz system operated with power >2.0 MW [B. N. Wan et al., Nucl. Fusion 57 (2017) 102019], leading to a reduction of the maximum power capability. With the new limiters, 4.6 GHz LH system, the main current drive (CD) and electron heating tool for EAST, can be operated with power >2.5 MW routinely. Long-pulse operation up to 100 s with 4.6 GHz LH power of 2.4 MW was achieved in 2021 and the maximal temperature on the guard limiters measured by an infrared (IR) camera was about 540 C, much below the permissible value of tungsten material (~1200 C). A discharge with a duration of 1056 s was achieved and the 4.6 GHz LH energy injected into the plasma was up to 1.05 GJ. Secondly, the fully-activemultijunction (FAM) launcher of 2.45 GHz system was upgraded to a passive-active-multijunction (PAM), for which the density of optimum coupling was relatively low (below the cut-off value). Good coupling with reflection coefficient ~3% has been achieved with plasma-antenna distance up to 11 cm for the new PAM. Finally, in order to eliminate the effect of ion cyclotron range of frequencies (ICRF) wave on 4.6 GHz LH wave coupling, the location of the ICRF launcher was changed to a port that is located 157.5 toroidally from the 4.6 GHz LH system and is not magnetically connected

Dynamic Threshold Switching Behavior of Ge[sub 2]Sb[sub 2]Te[sub 5] and Sb-doped Ge[sub 2]Sb[sub 2]Te[sub 5] Thin Films Using Scanning Electrical Nanoprobe

Zhou, P.,Shin, Y. C.,Choi, B. J.,Choi, S.,Hwang, C. S.,Lin, Y. Y.,Lv, H. B.,Yan, X. J.,Tang, T. A.,Chen, L. Y.,Chen, B. M. The Electrochemical Society 2007 Electrochemical and solid-state letters Vol.10 No.9

Measurement of the Secondary Neutron Emission Differential and Double-Differential Cross Sections between 20 and 30 MeV

X. C. Ruan,G. C. Chen,H. X. Huang,X. Li,Y. B. Nie,B. Zhou,Z. Y. Ma,J. Bao,Q. P. Zhong,Z. Y. Zhou,H. Q. Tang,J. S. Zhang,C. L. Lan,Y. L. Zhang,Y. M. Li 한국물리학회 2011 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.59 No.23

More and more interests have been paid to the differential and double-differential neutron emission cross sections (DX and DDX) at above 20 MeV region in recent years. At the China Institute of Atomic Energy (CIAE), a program to measure the DX and DDX between 20 and 30 MeV neutron energy region has been carried out. The d-T reaction neutron source was used to produce the 20 - 30 MeV neutrons with a tritium gas target at the HI-13 tandem accelerator at CIAE. The time-of-flight(TOF) technique was used to measure the neutron energy from about 1 MeV to 30 MeV with 3 BC501A liquid scintillators (with a size of Φ180 ×100 mm). A preliminary measurement has been carried out for ^9Be at 22 MeV. The measured data were analyzed by detailed Monte-Carlo simulation to perform the multiple scattering, flux attenuation and finite geometry corrections. The experimental details, the data analysis, the progress, the results and the future plan will be presented in this report.

Bioprocessing for elimination antibiotics and hormones from swine wastewater

Cheng, D.L.,Ngo, H.H.,Guo, W.S.,Liu, Y.W.,Zhou, J.L.,Chang, S.W.,Nguyen, D.D.,Bui, X.T.,Zhang, X.B. Elsevier 2018 Science of the Total Environment Vol.621 No.-

<P><B>Abstract</B></P> <P>Antibiotics and hormones in swine wastewater have become a critical concern worldwide due to the severe threats to human health and the eco-environment. Removal of most detectable antibiotics and hormones, such as sulfonamides (SAs), SMs, tetracyclines (TCs), macrolides, and estrogenic hormones from swine wastewater utilizing various biological processes were summarized and compared. In biological processes, biosorption and biodegradation are the two major removal mechanisms for antibiotics and hormones. The residuals in treated effluents and sludge of conventional activated sludge and anaerobic digestion processes can still pose risks to the surrounding environment, and the anaerobic processes' removal efficiencies were inferior to those of aerobic processes. In contrast, membrane bioreactors (MBRs), constructed wetlands (CWs) and modified processes performed better because of their higher biodegradation of toxicants. Process modification on activated sludge, anaerobic digestion and conventional MBRs could also enhance the performance (e.g. removing up to 98% SMs, 88.9% TCs, and 99.6% hormones from wastewater). The hybrid process combining MBRs with biological or physical technology also led to better removal efficiency. As such, modified conventional biological processes, advanced biological technologies and MBR hybrid systems are considered as a promising technology for removing toxicants from swine wastewater.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Biological antibiotics and hormones treatment process for swine wastewater was reviewed. </LI> <LI> Mechanisms of antibiotics/hormones removal can be biosorption and biodegradation. </LI> <LI> Conventional AS and AD are ineffective for antibiotics & hormones removal. </LI> <LI> MBRs/hybrid MBRs and constructed wetlands can improve toxicants removal. </LI> <LI> Modified conventional bioreactors are also efficient in antibiotics and hormones removal. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Phospholipase C delta 1 is a novel 3p22.3 tumor suppressor involved in cytoskeleton organization, with its epigenetic silencing correlated with high-stage gastric cancer

Hu, X -T,Zhang, F -B,Fan, Y -C,Shu, X -S,Wong, A H Y,Zhou, W,Shi, Q -L,Tang, H -M,Fu, L,Guan, X -Y,Rha, S Y,Tao, Q,He, C Macmillan Publishers Limited 2009 Oncogene Vol.28 No.26

Located at the important tumor suppressor locus, 3p22, PLCD1 encodes an enzyme that mediates regulatory signaling of energy metabolism, calcium homeostasis and intracellular movements. We identified PLCD1 as a downregulated gene in aerodigestive carcinomas through expression profiling and epigenetic characterization. We found that PLCD1 was expressed in all normal adult tissues but low or silenced in 84% (16/19) gastric cancer cell lines, well correlated with its CpG island (CGI) methylation status. Methylation was further detected in 62% (61/98) gastric primary tumors, but none of normal gastric mucosa tissues. PLCD1 methylation was significantly correlated with tumor high stage. Detailed methylation analysis of 37 CpG sites at the PLCD1 CGI by bisulfite genomic sequencing confirmed its methylation. PLCD1 silencing could be reversed by pharmacological demethylation with 5-aza-2′-deoxycytidine, indicating a direct epigenetic silencing. Ectopic expression of PLCD1 in silenced gastric tumor cells dramatically inhibited their clonogenicity and migration, possibly through downregulating MMP7 expression and hampering the reorganization of cytoskeleton through cofilin inactivation by phosphorylation. Thus, epigenetic inactivation of PLCD1 is common and tumor-specific in gastric cancer, and PLCD1 acts as a functional tumor suppressor involved in gastric carcinogenesis.Oncogene (2009) 28, 2466–2475; doi:10.1038/onc.2009.92; published online 18 May 2009

Effects of Probiotic-vitacogen and β1-4 Galacto-oligosaccharides Supplementation on Methanogenesis and Energy and Nitrogen Utilization in Dairy Cows

Mwenya, B.,Zhou, X.,Santoso, B.,Sar, C.,Gamo, Y.,Kobayashi, T.,Takahashi, J. Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.3

The effects of probiotic-vitacogen and galacto-oligosaccharides (GOS) supplementation on methanogesis, energy and nitrogen utilization in replacement dairy cows were evaluated. A basal diet comprising orchardgrass hay, lucerne hay cube and concentrate (2:2:1, DM basis) were fed with or without supplements to four cows at $80g\;DM/kgBW^{0.75}$per day in a $4{\times}4$ Latin square arrangement. The four treatments were; 1) basal diet, 2) basal diet plus 100 g probiotic-vitacogen, 3) basal diet plus 50 g GOS, 4) basal diet plus 50 g GOS and 100 g probiotic-vitacogen. Nutrient apparent digestibility was not altered by the effect of supplementation. Nitrogen intake was significantly (p<0.001) higher for the two vitacogen-supplemented diets compared to control and GOS supplemented diets. However, vitacogen supplemented diets had numerically higher fecal and urinary nitrogen losses, thereby, having lower nitrogen retention compared to control and GOS supplemented diets. Gross energy intake was also significantly (p<0.05) higher for vitacogen-supplemented diets compared to control and GOS diets, however, due to higher losses in feces, urine, methane and heat, GOS supplemented diet had numerically higher energy retention. There was an 11% reduction in methane emission (liters/day) in GOS supplemented diet compared to control diet. However, the combination of GOS with vitacogen resulted in an increased methane emission. When expressed per unit of animal production (g/kg live-weight gain), methane production tended to be lower in vitacogensupplemented diets compared to control and GOS diets. The supplementation of replacement dairy cows with GOS reduced methane emission (liters/day), while, vitacogen supplementation reduced methane emission per unit animal production. The two feed supplements may contribute to the abatement of methane as a greenhouse gas.

Effects of Partial or Total Replacement of Maize with Alternative Feed Source on Digestibility, Growth Performance, Blood Metabolites and Economics in Limousin Crossbred Cattle

Shi, F.H.,Fang, L.,Meng, Q.X.,Wu, H.,Du, J.P.,Xie, X.X.,Ren, L.P.,Zhou, Z.M.,Zhou, B. Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.10

Increasing cost and scarcity of maize has stimulated the use of alternative feed sources (AFS) in the diets of cattle. In this study, we investigated the effects of partial or total replacement of maize on nutrient digestibility, growth performance, blood metabolites, and economics in Limousin crossbred feedlot cattle. Forty-five $Limousin{\times}Luxi$ crossbred bulls were randomly assigned to the three treatment groups, orthodox diet (OD; 45.0% maize), partial replacement diet (PRD; 15% maize, 67% AFS), total replacement diet (TRD; 0% maize, 100% AFS). The growth feeding trial lasted for 98 days. Dry matter intake (DMI) and average daily gain (ADG) were recorded. The digestion trial was carried out after the end of the growth trial. Total faeces and feed samples were measured daily. Digestibilities of dry matter (DM) and organic matter (OM), crude protein (CP), neutral detergent fiber (NDF) and acid detergent fiber (ADF) were calculated. After the feeding trial, blood metabolites were measured in 12 animals from each group. Initial and final body weights did not differ significantly among treatment groups (p>0.05). The ADG and DMI were 1.72 and 8.66, 1.60 and 9.10, and 1.40 and 9.11 kg/d for OD, PRD, and TRD, respectively. The PRD and TRD exhibited lower ADG (p<0.01) and higher DMI (p<0.01) than OD. The DMI (%body weight) was comparable between groups (p>0.5). Feed efficiency of PRD and TRD were lower than OD (p<0.01). The DM digestibility decreased with reduced level of maize (p = 0.10), OM digestibility was higher in OD (p<0.05), and CP, NDF and ADF digestibilities were similar for all groups (p>0.05). Blood urea nitrogen (mg/dL) in PRD and TRD was higher than OD (p<0.01), while other blood parameters did not differ significantly. Feed costs ($/head/d) were 1.49, 0.98, and 0.72 for OD, PRD, and TRD, respectively (p<0.01). Feed costs per kg gain ($) were significantly lower for PRD (0.63) and TRD (0.54) than OD (0.89; p<0.01). Overall profit ($/head) and daily profit ($/head/d) did not differ significantly between treatments (p>0.05), although TRD showed the highest economic benefits overall (p<0.01). While a traditional diet maximized the growth rate, partial or total replacement of dietary maize with AFS proved economically feasible due to their lower costs and comparable nutrient digestibilities of DM, CP, NDF, and ADF. Partial replacement may prove economically competitive in the current situation of China.