High Mobility Group Box 1 Protein Is Methylated and Transported to Cytoplasm in Clear Cell Renal Cell Carcinoma

Wu, Fei,Zhao, Zuo-Hui,Ding, Sen-Tai,Wu, Hai-Hu,Lu, Jia-Ju Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10

Background: The high mobility group box 1 (HMGB1) protein is a widespread nuclear protein present in most cell types. It typically locates in the nucleus and functions as a nuclear cofactor in transcription regulation. However, HMGB1 can also localize in the cytoplasm and be released into extracellular matrix, where it plays critical roles in carcinogenesis and inflammation. However, it remains elusive whether HMGB1 is relocated to cytoplasm in clear cell renal cell carcinoma (ccRCC). Methods: Nuclear and cytoplasmic proteins were extracted by different protocols from 20 ccRCC samples and corresponding adjacent renal tissues. Western blotting and immunohistochemistry were used to identify the expression of HMGB1 in ccRCC. To elucidate the potential mechanism of HMGB1 cytoplasmic translocation, HMGB1 proteins were enriched by immunoprecipitation and analyzed by mass spectrometry (MS). Results: The HMGB1 protein was overexpressed and partially localized in cytoplasm in ccRCC samples (12/20, 60%, p<0.05). Immunohistochemistry results indicated that ccRCC of high nuclear grade possess more HMGB1 relocation than those with low grade (p<0.05). Methylation of HMGB1 at lysine 112 in ccRCC was detected by MS. Bioinformatics analysis showed that post-translational modification might affect the binding ability to DNA and mediate its translocation. Conclusion: Relocation of HMGB1 to cytoplasm was confirmed in ccRCC. Methylation of HMGB1 at lysine 112 might the redistribution of this cofactor protein.

An Intelligent Renewable Energy Distribution Strategy for Off-line Fare Meter

Sen-Tung Wu,Yong-Ye Lin,Feng-Chen Wu,Pin-He Liu,Yen-Chih Liu,Ching-Chun Chuang 전력전자학회 2023 ICPE(ISPE)논문집 Vol.2023 No.-

This paper designs an intelligent solar energy storage system for off-line electronic devices, which collects solar energy through solar panels and stores it in a storage system. The energy storage components include a set of supercapacitor and two sets of lithium-ion battery packs. An intelligent energy storage and discharging optimization strategy is adopted to allocate energy effectively between the supercapacitor and lithium-ion battery packs, which enabling the two sets of lithium-ion battery packs to alternate between energy storage to achieve uninterrupted power supply for the load system. The experimental results show that the total conversion efficiency of the intelligent power distribution system (including the self-powered system) can reach up to 89.32%.

A Collaborative Filtering Recommender System Integrated with Interest Drift Based on Forgetting Function

Wu Sen,Zhang Xiaonan,Du Yannan 보안공학연구지원센터 2015 International Journal of u- and e- Service, Scienc Vol.8 No.4

Association of Oral Contraceptives Use and Lung Cancer Risk among Women: an Updated Meta-analysis Based on Cohort and Case-control Studies

Wu, Wei,Yin, Zhi-Hua,Guan, Peng,Ren, Yang-Wu,Zhou, Bao-Sen Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.3

Background: Previous studies on the association of oral contraceptives (OC) use and lung cancer generated inconsistent findings. The aim of this study was to confirm any definite correlation between OC use and lung cancer risk. Methods: Publications were reviewed and obtained through PubMed and EMBASE databases literature search up to November, 2013. Reference lists from retrieved articles were also reviewed. The language of publication was restricted to English. A meta-analysis was performed to evaluate the association by calculating pooled odds ratios (ORs) and 95% confidence intervals (CIs). Results: A total of 14 studies consisting of 9 case-control studies and 5 cohort studies were finally included in this meta-analysis. There was no significant association observed between OC use and lung cancer risk in the overall analysis (OR=0.91; 95% CI=0.81-1.03). There was a significant protective effect in Europe (OR=0.74; 95% CI=0.60-0.91) and a borderline significant protective effect with an adenocarcinoma histology (OR=0.90; 95% CI=0.80-1.01) in subgroup analyses. No association was observed for methodological quality of study, study design, smoking status and case number of study. Conclusion: This meta-analysis suggests that OC use is not likely to be associated with the risk of lung cancer at all. While a significant protective effect of OC use on lung cancer was observed in Europe, interpretation should be cautious because of the potential biases of low-quality studies. At the same time, more attention should be paid to the possible association of OC use with adenocarcinoma of lung. Our findings require further research, with well-conducted and large-scale epidemiological studies to confirm effects of OC use on lung cancer.

Scientific Collaboration Networks in China’s System Engineering Subject

Sen Wu,Jiaye Wang,Xiaodong Feng,Dan Lu 보안공학연구지원센터(IJUNESST) 2013 International Journal of u- and e- Service, Scienc Vol.6 No.6

Clustering Algorithm for Incomplete Data Sets with Mixed Numeric and Categorical Attributes

Wu Sen,Chen Hong,Feng Xiaodong 보안공학연구지원센터 2013 International Journal of Database Theory and Appli Vol.6 No.5

P53 Arg72Pro and MDM2 SNP309 Polymorphisms Cooperate to Increase Lung Adenocarcinoma Risk in Chinese Female Non-smokers: A Case Control Study

Ren, Yang-Wu,Yin, Zhi-Hua,Wan, Yan,Guan, Peng,Wu, Wei,Li, Xue-Lian,Zhou, Bao-Sen Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.9

Background: Cell cycle deregulation is a major component of carcinogenesis. The p53 tumor suppressor gene plays an important role in regulating cell cycle arrest, and mouse double minute 2 (MDM2) is a key regulator of p53 activity and degradation. Abnormal expression of p53 and MDM2 occurs in various cancers including lung cancer. Methods: We investigated the distribution of the p53 Arg72Pro (rs1042522) and MDM2 SNP309 (rs2279744) genotypes in patients and healthy control subjects to assess whether these single nucleotide polymorphisms (SNPs) are associated with an increased risk of lung adenocarcinomas in Chinese female non-smokers. Genotypes of 764 patients and 983 healthy controls were determined using the TaqMan SNP genotyping assay. Results: The p53 Pro/Pro genotype (adjusted OR = 1.55, 95% CI = 1.17-2.06) significantly correlated with an increased risk of lung adenocarcinoma, compared with the Arg/Arg genotype. An increased risk was also noted for MDM2 GG genotype (adjusted OR = 1.68, 95% CI = 1.27-2.21) compared with the TT genotype. Combined p53 Pro/Pro and MDM2 GG genotypes (adjusted OR = 2.66, 95% CI = 1.54-4.60) had a supermultiplicative interaction with respect to lung adenocarcinoma risk. We also found that cooking oil fumes, fuel smoke, and passive smoking may increase the risk of lung adenocarcinomas in Chinese female non-smokers who carry p53 or MDM2 mutant alleles. Conclusions: P53 Arg72Pro and MDM2 SNP309 polymorphisms, either alone or in combination, are associated with an increased lung adenocarcinoma risk in Chinese female non-smokers.

Association analysis of the SNP (rs345476947) in the FUT2 gene with the production and reproductive traits in pigs

Haifei Wang,Sen Wu,Jiayun Wu,Shouyong Sun,Shenglong Wu,Wen Bin Bao 한국유전학회 2018 Genes & Genomics Vol.40 No.2

The FUT2 gene was considered as an important candidate for pathogenic infections, while the potential associations between this gene and the production and reproductive traits of pigs have not been explored. In this study, we detected the genetic variants of porcine FUT2 gene and analyzed the associations of the polymorphisms with FUT2 mRNA expression and production and reproductive traits (age at 100 kg, backfat thickness at 100 kg, eye muscle thickness, the number of newborn piglets, the number of weaned piglets, and birth weight) in 100 Large White sows. One single nucleotide polymorphism (SNP) (rs345476947, C→T) in the intron of FUT2 and three genotypes (TT, CT and CC) were determined. Association analysis revealed significant associations between this SNP with the number of newborn piglets and weaned piglets. Furthermore, individuals with the TT genotype had significantly higher numbers of newborn piglets and weaned piglets than those with the CC genotype (P < 0.05). Quantitative PCR analysis showed that FUT2 expression in individuals with CC genotype was significantly higher than those with TT and CT genotypes in the liver and lymph gland (P < 0.05) and higher than that of CT in the spleen, kidney, and duodenum (P < 0.05). These findings indicated that the TT genotype may be a favorable genotype for the reproductive traits of pigs. Our study revealed the genetic variants of the FUT2 gene and identified a promising candidate SNP (rs345476947) associated with the reproductive traits, which has the potential to be applied in selective breeding of pigs.

Investigation on Driver’s Lower Limb Injury Based on 3D Active Muscle Model During Offset Frontal Impact

Wu Chen,Sen Xiao,Zhidong Qu,Xinran Liu 한국정밀공학회 2021 International Journal of Precision Engineering and Vol.22 No.5

Lower limb injuries occupy a high percentage of the traffic accident injuries among road users. However, the injury outcomes in accident statistics is somehow different from the results in cadaver tests. The purpose of this study is to determine the influence of active muscle function on the kinetics result of lower limb injuries during emergency braking in car frontal collision. Based on a human body numerical model regrading a Chinese 50th percentile male lower extremity with active muscles, frontal collisions under different overlaps were established. Then, by comparing the peak forces and moments of the femur and tibia, the dynamic responses of lower limbs under different load conditions were determined. Furthermore, the distribution of stress indicators was analyzed to determine the influence of active muscle and collision overlaps on the injury outcomes at a micro level. Results show that active muscle has a significant effect on the lower limbs, which are particularly affected by the increase of bending moments. The moment caused by the active muscles in the right tibia is 3.7 times greater than that of the femur. Moreover, the effect of active muscle is more significant in small overlap frontal impact. In the 25% overlap collision, the moment caused by the active muscles of the tibia increase by 1.2 times that of the 40% off set collision. In conclusion, this study can provide an explanation on the differences of lower limb injuries between the cadaveric tests and traffic accidents statistics.

Theoretical study on the hydrolysis mechanism of N,N-dimethyl-N′-(2-oxo-1, 2-dihydro-pyrimidinyl)formamidine: Water-assisted mechanism and cluster-continuum model

Wu, Yong,Jin, Lu,Xue, Ying,Xie, Dai Qian,Kim, Chan Kyung,Guo, Yong,Yan, Guo Sen Wiley Subscription Services, Inc., A Wiley Company 2008 Journal of computational chemistry Vol.29 No.8

<P>The hydrolysis reaction of N,N-dimethyl-N′-(2-oxo-1, 2-dihydro-pyrimidinyl)formamidine (DMPFA), a model compound of the antivirus drug amidine-3TC (3TC = 2′, 3′-dideoxy-3′-thiacytidine), is investigated by the hybrid density functional theory B3LYP/6-31+G (d,p) method. The hydrolysis reaction of the title compound is predicted to undergo via two pathways, each of which is a stepwise process. Path A is the addition of H<SUB>2</SUB>O to the C&n.dbond;N double bond in the amidine group to form a tetrahedral structure in its first step, and then the transfer of the H atom of hydroxyl leads to the corresponding products via four possible channels. Path B simultaneously involves the nucleophilic attack of H<SUB>2</SUB>O to the C atom of the C&n.dbond;N bond and the proton transfer to the N atom of amino group leading to the cleavage of the C&n.bond;N single bond in the amidine group. The results indicate that path A is more favorable than path B in the gas phase. Moreover, to simulate the title reaction in aqueous solution, water-assisted mechanism and the cluster-continuum model, based on the SCRF/CPCM model, are taken into account in our work. The results indicate that it is rational for two water molecules served as a bridge to assist in the first step of path A and that cytosine rather than the cytosine-substituted formamide should be released from the tetrahedral intermediate via s six-membered cycle transition state (channel 2). Our calculations exhibit that the process toward the tetrahedral intermediate is the rate-determining step both in the gas phase and in aqueous solution. © 2007 Wiley Periodicals, Inc. J Comput Chem, 2008</P> <B>Graphic Abstract</B> <P> <img src='wiley_img/01928651-2008-29-8-JCC20883-gra001.gif' alt='wiley_img/01928651-2008-29-8-JCC20883-gra001'> </P>