Macrophages Promote Coal Tar Pitch Extract-induced Tumorigenesis of BEAS-2B Cells and Tumor Metastasis in Nude Mice Mediated by AP-1

Zhang, Peng,Jin, Yue-Fei,Zhang, Qiao,Wu, Yi-Ming,Wu, Wei-Dong,Yao, Wu,Wu, Yong-Jun,Li, Zhi-Tao,Zhao, Yong,Liu, Yu,Feng, Fei-Fei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.12

Background: We sought to evaluate the role of tumor associated macrophages (TAMs) on the promotion of coal tar pitch extract (CTPE)-induced tumorigenesis of human bronchial epithelial cells (BEAS-2B) and tumor metastasis in nude mice, and related mechanisms. Materials and Methods: BEAS-2B cells were first treated with 2.4 mg/mL CTPE for 72 hours. After removal of CTPE, the cells were continuously cultured and passaged using trypsin-EDTA. THP-1 cells were used as macrophage-like cells. BEAS-2B cells under different conditions (n=6/group) were injected into the back necks of nude mice, and alterations of tumor xenograft growth, indicative of tumorigenicity, and tumor metastasis were determined. Pathological changes (tumor nests and microvascular lesions) of HE-stained tumor tissues were also evaluated. The expression of AP-1(c-Jun) in xenografts and metastatic tumors was determined using immunohistochemistry. Results: Tumor size and weight in nude mice transplanted with the mixture of CTPE-induced passage 30 BEAS-2B and THP-1 cells (2:1) were increased compared to those from the CTPE-treated BEAS-2B cells at passage 30 alone at different observation time points. Tumor metastasis to lymph nodes and liver was only detected after transplantation of a mixture the two kinds of cells. The numbers of tumor nests and microvascular lesions, and the expression levels of AP-1 (c-Jun) in tumors from the mixture of two kinds of cells were increased apparently in contrast to those in tumor from the CTPE-treated BEAS-2B cells of passage 30 alone. In addition, there was positive correlation between AP-1 (c-Jun) expression level and the number of microvascular lesions, or between AP-1 (c-Jun) expression level and tumor metastasis in these two groups. Conclusions: TAMs not only facilitate tumorigenesis transformation of CTPE-induced BEAS-2B cells, but also promote tumor growth, angiogenesis and metastasis in nude mice in vivo, which may be mediated by AP-1.

Thin Film Fabrications : PREPARATION AND CHARACTERIZATION OF CdTe AND HgCdTe BY HOT WALL MOCVD

Peng Rui Wu,Ding Yong Qing 대한금속재료학회 ( 구 대한금속학회 ) 1995 Advanced Material and Processing Vol.1 No.-

Magnetic Resonance Image Manifestations of the Atypical Meningioma

Wu, Qing-Wu,Yan, Rui-Fang,Li, Qiang,Hu, Ying,Zhou, Feng-Mei,Ren, Ji-Peng,Yang, Rui-Min,Zhang, Yan Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11

Through retrospective analysis of 13 cases of magnetic resonance image (MRI) manifestations of atypical meningiomas confirmed by operation and pathology in the First Affiliated Hospital of Xinxiang Medical University, the objective of this study was to evaluate the diagnostic value of MRI in order to improve the accuracy rate of preoperative diagnosis. In this retrospective analysis of MRI findings for atypical meningiomas in First Affiliated Hospital of Xinxiang Medical University from January to July in 2012, the location, morphology and tumor signals and other tumor imaging characteristics were covered. In 13 cases of atypical meningioma patients of this group, most tumors were located at typical sites (10/13), mainly the falx cerebri, parasagittal, convexity, saddle area. Only two cases were at atypical locations, 1 in the cerebellar hemisphere and 1 in a lateral ventricle. Most of the tumors showed T1 and T2 isointensity signals, and necrosis, calcification, and peritumoral edema were always featured. DWI showed isointensity in 11 cases (11/13), and hyperintensity in 2. Some 9 cases had dural tail signs, 12 had accurate positioning (12/13), and 2 were postoperative recurrences. MRI has high value in the diagnosis of atypical meningiomas, with important roles in early clinical diagnosis, treatment and prognosis evaluation.

Protein-protein Interaction Network Analyses for Elucidating the Roles of LOXL2-delta72 in Esophageal Squamous Cell Carcinoma

Wu, Bing-Li,Zou, Hai-Ying,Lv, Guo-Qing,Du, Ze-Peng,Wu, Jian-Yi,Zhang, Pi-Xian,Xu, Li-Yan,Li, En-Min Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.5

Lysyl oxidase-like 2 (LOXL2), a member of the lysyl oxidase (LOX) family, is a copper-dependent enzyme that catalyzes oxidative deamination of lysine residues on protein substrates. LOXL2 was found to be overexpressed in esophageal squamous cell carcinoma (ESCC) in our previous research. We later identified a LOXL2 splicing variant LOXL2-delta72 and we overexpressed LOXL2-delta72 and its wild type counterpart in ESCC cells following microarray analyses. First, the differentially expressed genes (DEGs) of LOXL2 and LOXL2-delta72 compared to empty plasmid were applied to generate protein-protein interaction (PPI) sub-networks. Comparison of these two sub-networks showed hundreds of different proteins. To reveal the potential specific roles of LOXL2- delta72 compared to its wild type, the DEGs of LOXL2-delta72 vs LOXL2 were also applied to construct a PPI sub-network which was annotated by Gene Ontology. The functional annotation map indicated the third PPI sub-network involved hundreds of GO terms, such as "cell cycle arrest", "G1/S transition of mitotic cell cycle", "interphase", "cell-matrix adhesion" and "cell-substrate adhesion", as well as significant "immunity" related terms, such as "innate immune response", "regulation of defense response" and "Toll signaling pathway". These results provide important clues for experimental identification of the specific biological roles and molecular mechanisms of LOXL2-delta72. This study also provided a work flow to test the different roles of a splicing variant with high-throughput data.

A New Path of Peaceful Revolution : The South Korea's National Image Strategy and Its Impact on the Reunification of Korean Peninsula

Peng Wu 한국평화연구학회 2016 한국평화연구학회 학술회의 Vol.2016 No.07

Technological, Pedagogical, and Content Knowledge in Teaching Translation : Representation of Chinese MTI Teachers

Peng WU 한국외국어대학교 통번역연구소 2019 한국외국어대학교 통번역연구소 학술대회 Vol.2019 No.07

Informed by the importance of technological knowledge for conducting effective technology-enhanced instruction, this study investigates translation teachers’ technological, pedagogical, and content knowledge (TPACK) to understand seven TPACK components and their synthesized pattern. A survey was conducted to assess translation teachers’ performance among 98 Chinese teachers in MTI program. A regression analysis was made to reveal the interrelationship among the seven TPACK components. The Survey shows the translation teachers’ technological knowledge is the highest component among all the seven TPACK components. Regression analysis reveals that the three core components of technological, pedagogical and content knowledge mutually influence TPACK in different synthesized patterns. The results will inform translation teachers of the effective way to enhance their instructional strategies with IT devices and platform.

An Improved Two-Dimensional Run-Length Encoding Scheme and Its Application

Peng Wu,Shunping Zhou,Bo wan,Fang Fang,Sha Zhou 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.4

In this paper, we propose an improved two-dimensional run-length encoding (I2DRLE) scheme for representing grayscale images. Conventional 2D run-length encoding scheme is simple and effective that has been widely used, while it is not suitable to represent non-block images. Our approach is a new data compression algorithm inspired by 2D run-length encoding and quadtree, which apply some predefined patterns to represent various data and can sharply reduce the number of blocks in image representation. Experimental results show that this method is an effective lossless grayscale image encoding scheme.

Segmentation of Leaf Images Based on the Active Contours

Wu Peng,Li Wenlin,Song Wenlong 보안공학연구지원센터 2015 International Journal of u- and e- Service, Scienc Vol.8 No.6

Leaves contain important genetic information which can be used as a basis for the identification of plants. As a first step of modeling virtual three-dimensional plant, how to extract visual characteristic information form leaf images has great significance. We propose an optimized C-V model in this paper, which can detect objects in homogeneous regions of given leaf images and speed up running time. The new method combines local information with global information and optimizes the defect that SDF needs to be reconstructed partially so that the energy function is improved. Experimental results show that our algorithm can stop active contours on the correct boundary, get accurate image segmentation, and the speed is more than 1.5 times faster to C-V model.

中国普通高校公共艺术教育课程现状调查研究 ― 基于大学生的课程实践体验

Wu Peng,이영 한국중국언어문화연구회 2021 한중언어문화연구 Vol.- No.59

公共艺术教育着眼于促进学生的身心全面发展,它是高校进行素质教育的重要途径。然而,受各种历史原因和功利化办学思想的影响,中国高校公共艺术教育在整个学校教育中不受重视,长期被“边缘化”、“模糊化”,处于十分薄弱的环节。公共艺术教育作为普通高校素质教育的一个重要组成部分,对于促进大学生的全面发展有着十分重要的作用。近年来,随着中国教育部颁布了一系列关于促进公共艺术教育发展的政策,公共艺术教育课程在中国高校教育体系中的地位日益凸显,并取得飞速发展。然而,尽管目前对于中国的公共艺术教育课程的研究正在逐渐增加,但是关于公共艺术教育课程发展中存在问题的分析尚且不足。对此,笔者结合相关文献研究,以课程设置四要素,即,课程目标、课程内容、课程实施和教学评价四个维度为切入点,对普通高校公共艺术教育课程现状进行问卷调查。调查结果发现,中国高校对公共艺术教育课程重要性认识不足、课程目标认识不到位;课程内容特色不明显,教育内容与学生需求相对脱节;教学方式不够多元化、不能满足学生的学习需求;课程评价方式单一、评价机制不够完善等诸多问题。本文结合调查结果,对目前中国高校公共艺术教育课程发展现状中存在的问题给与相关建议与对策,以期促进中国高校公共艺术教育课程的发展。

Comprehensive Bioinformation Analysis of the MRNA Profile of Fascin Knockdown in Esophageal Squamous Cell Carcinoma

Wu, Bing-Li,Luo, Lie-Wei,Li, Chun-Quan,Xie, Jian-Jun,Du, Ze-Peng,Wu, Jian-Yi,Zhang, Pi-Xian,Xu, Li-Yan,Li, En-Min Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

Background: Fascin, an actin-bundling protein forming actin bundles including filopodia and stress fibers, is overexpressed in multiple human epithelial cancers including esophageal squamous cell carcinoma (ESCC). Previously we conducted a microarray experiment to analyze fascin knockdown by RNAi in ESCC. Method: In this study, the differentially expressed genes from mRNA expression profilomg of fascin knockdown were analyzed by multiple bioinformatics methods for a comprehensive understanding of the role of fascin. Results: Gene Ontology enrichment found terms associated with cytoskeleton organization, including cell adhesion, actin filament binding and actin cytoskeleton, which might be related to fascin function. Except GO categories, the differentially expressed genes were annotated by 45 functional categories from the Functional Annotation Chart of DAVID. Subpathway analysis showed thirty-nine pathways were disturbed by the differentially expressed genes, providing more detailed information than traditional pathway enrichment analysis. Two subpathways derivated from regulation of the actin cytoskeleton were shown. Promoter analysis results indicated distinguishing sequence patterns and transcription factors in response to the co-expression of downregulated or upregulated differentially expressed genes. MNB1A, c-ETS, GATA2 and Prrx2 potentially regulate the transcription of the downregulated gene set, while Arnt-Ahr, ZNF42, Ubx and TCF11-MafG might co-regulate the upregulated genes. Conclusions: This multiple bioinformatic analysis helps provide a comprehensive understanding of the roles of fascin after its knockdown in ESCC.