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Wolff Bernard J.,Gaines Anna,Conley Andrew B.,Norris Emily,Rishishwar Lavanya,Chande Aroon T.,Yang Eungi,Diaz Maureen H.,Winchell Jonas M. 대한진단검사의학회 2023 Annals of Laboratory Medicine Vol.43 No.4
We developed and assessed the performance of a new multiplex real-time PCR assay for the detection of all Chlamydia species and simultaneous differentiation of Chlamydia psittaci and Chlamydia pneumoniae—two important human respiratory pathogens—in human clinical specimens. Next-generation sequencing was used to identify unique targets to design real-time PCR assays targeting all Chlamydia species, C. psittaci, and C. pneumoniae. To validate the assay, we used a panel of 49 culture isolates comprising seven C. psittaci genotypes, eight C. pneumoniae isolates, seven other Chlamydia species, and 22 near-neighbor bacterial and viral isolates, along with 22 specimens from external quality assessment (EQA) panels and 34 nasopharyngeal and oropharyngeal swabs and cerebrospinal fluid, stool, and sputum specimens previously identified as positive or negative for C. psittaci or C. pneumoniae. The assays were 100% specific, with limits of detection of 7.64– 9.02 fg/μL. The assay results matched with historical assay results for all specimens, except for one owing to the increased sensitivity of the new C. psittaci assay; the results of the EQA specimens were 100% accurate. This assay may improve the timely and accurate clinical diagnosis of Chlamydia infections and provide a greater understanding of the burden of disease caused by these agents.
2SG-1 Circular Economy of Mobile Phones and Li-Ion Batteries
( Fabian Wolff ) 한국공업화학회 2017 한국공업화학회 연구논문 초록집 Vol.2017 No.1
Electric and electronic devices have often a very short lifespan, which raises the question of their end of life treatment. While the level of product complexity is increasing every year, the recycling methods struggle to keep up with the technological progress and are lagging behind. Two waste fractions with very high contents of valuable materials are mobile phones and Li-Ion batteries. The Fraunhofer Institute aims to improve the recycling of mobile phones by a combination of an electro hydraulic fragmentation (EHF) in combination with the solvent based CreaSolv<sup>®</sup> Process. The CreaSolv<sup>®</sup> Process was also applied to improve the recycling of Li-Ion batteries. In a project we showed the possibility to retrieve the housing polymers, the separator and the binder PVDF while making the active mass fluorine-free and accessible. This makes downstream recovery of Co easier and more cost effective.