Long Non-Coding RNA TUG1 Attenuates Insulin Resistance in Mice with Gestational Diabetes Mellitus via Regulation of the MicroRNA-328-3p/SREBP-2/ERK Axis

Xuwen Tang,Qingxin Qin,Wenjing Xu,Xuezhen Zhang 대한당뇨병학회 2023 Diabetes and Metabolism Journal Vol.47 No.2

Background: Long non-coding RNAs (lncRNAs) have been illustrated to contribute to the development of gestational diabetes mellitus (GDM). In the present study, we aimed to elucidate how lncRNA taurine upregulated gene 1 (TUG1) influences insulin resistance (IR) in a high-fat diet (HFD)-induced mouse model of GDM.Methods: We initially developed a mouse model of HFD-induced GDM, from which islet tissues were collected for RNA and protein extraction. Interactions among lncRNA TUG1/microRNA (miR)-328-3p/sterol regulatory element binding protein 2 (SREBP-2) were assessed by dual-luciferase reporter assay. Fasting blood glucose (FBG), fasting insulin (FINS), homeostasis model assessment of insulin resistance (HOMA-IR), HOMA pancreatic β-cell function (HOMA-β), insulin sensitivity index for oral glucose tolerance tests (ISOGTT) and insulinogenic index (IGI) levels in mouse serum were measured through conducting gain- and loss-of-function experiments.Results: Abundant expression of miR-328 and deficient expression of lncRNA TUG1 and SREBP-2 were characterized in the islet tissues of mice with HFD-induced GDM. LncRNA TUG1 competitively bound to miR-328-3p, which specifically targeted SREBP-2. Either depletion of miR-328-3p or restoration of lncRNA TUG1 and SREBP-2 reduced the FBG, FINS, HOMA-β, and HOMA-IR levels while increasing ISOGTT and IGI levels, promoting the expression of the extracellular signal-regulated kinase (ERK) signaling pathway-related genes, and inhibiting apoptosis of islet cells in GDM mice. Upregulation miR-328-3p reversed the alleviative effects of SREBP-2 and lncRNA TUG1 on IR.Conclusion: Our study provides evidence that the lncRNA TUG1 may prevent IR following GDM through competitively binding to miR-328-3p and promoting the SREBP-2-mediated ERK signaling pathway inactivation.

HACP2 : The Pre-processing Software Tool for the Hybrid Atomistic-Continuum Coupling Simulation

Qian Wang,Wenjing Yang,Fu Li,Xiaoguang Ren,Yuhua Tang 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.1

Fluid simulation is an important application of High Performance Computing. The hybrid atomistic-continuum coupling fluid simulation can effectively handle the contradictory of the reduction of the simulation scale and the increase of the computation load when trying to improve the accuracy. The pre-processing procedure of the coupling simulation is quite important in the whole simulation process and requires the support of an efficient, user-friendly interface. Under the demand of efficient coupling fluid simulation, in this paper we design and implement a visualized pre-processing framework HACP2 based on SALOME, for the unified molecular dynamic-computational fluid dynamics modeling and apply it into the coupling simulation process. The experimental verification indicates that our HACP2 framework can offer efficient, easy-to-use pre-processing for the hybrid atomistic-continuum coupling simulation, and effectively improve the efficiency of the coupling simulation.

A novel hydrothermal releasing synthesis of modified SiO2 material and its application in phenol removal process

Xinyu Yang,Xiaoyao Liu,Wenjing Tang,Yajun Gao,Huijuan Ni,Jianbin Zhang 한국화학공학회 2017 Korean Journal of Chemical Engineering Vol.34 No.3

A modified SiO2 material (MSM) was successfully synthesized by using Na2SiO3 and a novel CO2SM in the presence of CTAB through an innovative hydrothermal releasing treatment protocol. The MSM was systemically characterized using several techniques, including N2 adsorption-desorption isotherm, thermogravimetric analysis (TGA), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and X-ray diffraction (XRD). The CO2SM as a platform for CO2 capturing and releasing can be reversibly used for multiple cycles without significant loss of capabilities. The optimum conditions for the preparation of MSM were identified as follows: Na2SiO3 concentration, 0.2mol/L; hydrothermal temperature, 120 oC; CO2SM dosage, 6 g; CTAB concentration, 24 g/L, reaction time, 12 h. Additionally, the MSM exhibited high efficiency for the removal of phenol from aqueous solution: it showed a phenol adsorption capacity of 91.29mg/g when shaken with the aqueous phenol solution at 180 rpm for 1 h at 25 oC.

Effects of three drying methods on polyphenol composition and antioxidant activities of Litchi chinensis Sonn.

Si Tan,Jianmin Tang,Wenjing Shi,Zhuwei Wang,Yuanyuan Xiang,Tingwei Deng,Xiaoxu Gao,Wenfeng Li,Shengyou Shi 한국식품과학회 2020 Food Science and Biotechnology Vol.29 No.3

The aim of this study was to investigate the effects of three different drying methods, freeze drying (FD), vacuum drying (VD) and oven drying (OD) on phenolic contents and antioxidant activities of litchi fruits. 20 polyphenols were exactly identified in the litchi fruits by UPLC-QqQ/MS. Significant losses were observed in the contents of total polyphenols and antioxidant activities in the dried litchi when compared with the fresh litchi. Principle component analysis indicated that there was significant difference of phenolic component between the use of thermal drying (VD and OD) and FD. Our results suggest that FD is the optimum drying method for litchi fruits considering the content of total polyphenols and antioxidant activities.

Characterization of high-alumina coal fly ash based silicate material and its adsorption performance to CO2

Jianbin Zhang,Yinan Yan,Yajun Gao,Wenjing Tang,Qiang Li 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.4

Silicate material prepared from high-alumina coal fly ash (HACFA) was characterized by using XRD, SEM, FTIR spectroscopy, TGA-DSC, and elemental analysis. These spectral results show that the silicate material is mainly composed of eight elements--O, C, Si, Ca, Na, Mg, Al, and Fe, which exist as the formations of Ca2+, Na+, Mg2+, Al3+, Fe3+, SiO3 2−, and CO3 2−, and some adsorbed water and crystal water are determined in the silicate material. The material with surface area of 117.12m2/g shows a faveolate structure, and a pore size distribution of silicate material is calculated at 11.01 nm from the nitrogen desorption isotherm using the BJH model. When the material was used for CO2 adsorption at T=323.15 K and flow rate=95mL/min with 15.79% (vol) CO2, a dynamic adsorption capacity of CO2 on the surface of silicate material was found at 8.67mg/g and the adsorption values decreased weakly after seventeen recycling times. The investigation of dynamic adsorption behavior shows that the silicate material presents similar adsorption properties with commercial active carbon and stronger adsorption properties than commercial diatomite.

Dual-Responsive Cross-Linked Micelles from Amphiphilic Four-Arm Star Copolymers with Different Block Ratios for Triggering DOX Release

Yunwei Huang,Yanzhe Li,Zilun Tang,Qiuping Su,Tingting Liao,Yuxin Gu,Xiaofeng Lin,Xihong Zu,Wenjing Lin,Guobin Yi 한국고분자학회 2020 Macromolecular Research Vol.28 No.8

The four-arm star copolymers poly(methacrylic acid)-poly(2-hydroxyethyl methacrylate-disulfide~)-poly(poly(ethylene glycol) methyl ether methacrylate) (4AS-PMAAx-(PHEMA-SS~)y-PPEGMAz) with four different block ratios were synthesized and could self-assembled into cross-linked polymer micelles for the exploration of the structure-property relationship. The cross-linked polymer micelles in aqueous solution had low critical micelle concentration (CMC) values (1.9-4.6 mg/L), which exhibited better stability than non-cross-linked micelles. The CMC value decreased with the increase of the length of inner PMAA core and hydrophobic PHEMA cross-linked middle layer. The blank and doxorubicin (DOX)- loaded micelles with different block ratios were prepared by dialysis with the particle sizes of 120-240 nm. The longer inner PMAA core and cross-linked middle layer enhanced the drug loading content (DLC) results and led to relatively bigger particle sizes of polymer micelles. The in vitro DOX release data revealed that DOX-loaded micelles had low DOX cumulative release percentages of 18-37% after 110 h at pH 7.4, but up to 83-90% when introducing reductant GSH at pH 5.0. The 4AS-PMAA21.2-(PHEMASS~) 13.1-PPEGMA5.1 micelles with the longest PMAA core had the largest cumulative release of 90.1%. The DOX release process and mechanism of the micelles at different conditions fitted well with the semi-empirical equation. Overall, the results demonstrated that the block ratios and pH/redox-responsiveness of these four-arm star copolymers could be well-controlled and their self-assembled cross-linked micelles as anticancer drug carrier system could be improved by optimizing the different ratios.

ROCK1 induces dopaminergic nerve cell apoptosis via the activation of Drp1-mediated aberrant mitochondrial fission in Parkinson’s disease

Qian Zhang,Changpeng Hu,Jingbin Huang,Wuyi Liu,Wenjing Lai,Faning Leng,Qin Tang,Yali Liu,Qing Wang,Min Zhou,Fangfang Sheng,Guobing Li,Rong Zhang 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

Dopamine deficiency is mainly caused by apoptosis of dopaminergic nerve cells in the substantia nigra of themidbrain and the striatum and is an important pathologic basis of Parkinson’s disease (PD). Recent research has shownthat dynamin-related protein 1 (Drp1)-mediated aberrant mitochondrial fission plays a crucial role in dopaminergicnerve cell apoptosis. However, the upstream regulatory mechanism remains unclear. Our study showed that Drp1knockdown inhibited aberrant mitochondrial fission and apoptosis. Importantly, we found that ROCK1 was activated inan MPP+-induced PD cell model and that ROCK1 knockdown and the specific ROCK1 activation inhibitor Y-27632blocked Drp1-mediated aberrant mitochondrial fission and apoptosis of dopaminergic nerve cells by suppressing Drp1dephosphorylation/activation. Our in vivo study confirmed that Y-27632 significantly improved symptoms in a PDmouse model by inhibiting Drp1-mediated aberrant mitochondrial fission and apoptosis. Collectively, our findingssuggest an important molecular mechanism of PD pathogenesis involving ROCK1-regulated dopaminergic nerve cellapoptosis via the activation of Drp1-induced aberrant mitochondrial fission.

Doppler LIDAR Measurement of Wind in the Stratosphere

Jihui Dong,Hyunki Cha,김덕현,Sung Hoon Baik,Guocheng Wang,Lei Tang,Zhifeng Shu,Wenjing Xu,Dongdong Hu,Dongsong Sun 한국광학회 2010 Current Optics and Photonics Vol.14 No.3

A mobile direct detection Doppler LIDAR based on molecular backscattering for measurement of wind in the stratosphere has been developed in Hefei, China. First, the principle of wind measurement with direct detection Doppler LIDAR is presented. Then the configuration of the LIDAR system is described. Finally, the primary experimental results are provided and analyzed. The results indicate that the detection range of the designed Doppler LIDAR reached 50 km altitude, and there is good consistency between the molecular Doppler wind LIDAR(DWL) and the wind profile radar(WPR) in the low troposphere.