Pre-operative Predictive Factors for Intra-operative Pathological Lymph Node Metastasis in Rectal Cancers

Gao, Chun,Li, Jing-Tao,Fang, Long,Wen, Si-Wei,Zhang, Lei,Zhao, Hong-Chuan Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11

Background: A number of clinicopathologic factors have been found to be associated with pathological lymph node metastasis (pLNM) in rectal cancer; however, most of them can only be identified by expensive high resolution imaging or obtained after surgical treatment. Just like the Child-Turcotte-Pugh (CTP) and the model for end-stage liver disease (MELD) scores which have been widely used in clinical practice, our study was designed to assess the pre-operative factors which could be obtained easily to predict intra-operative pLNM in rectal cancer. Methods: A cohort of 469 patients who were treated at our hospital in the period from January 2003 to June 2011, and with a pathologically hospital discharge diagnosis of rectal cancer, were included. Clinical, laboratory and pathologic parameters were analyzed. A multivariate unconditional logistic regression model, areas under the curve (AUC), the Kaplan-Meier method (log-rank test) and the Cox regression model were used. Results: Of the 469 patients, 231 were diagnosed with pLNM (49.3%). Four variables were associated with pLNM by multivariate logistic analysis, age<60 yr (OR=1.819; 95% CI, 1.231-2.687; P=0.003), presence of abdominal pain or discomfort (OR=1.637; 95% CI, 1.052-2.547; P=0.029), absence of allergic history (OR=1.879; 95% CI, 1.041-3.392; P=0.036), and direct $bilirubin{\geq}2.60{\mu}mol/L$ (OR=1.540; 95% CI, 1.054-2.250; P=0.026). The combination of all 4 variables had the highest sensitivity (98.7%) for diagnostic performance. In addition, age<60 yr and direct $bilirubin{\geq}2.60{\mu}mol/L$ were found to be associated with prognosis. Conclusion: Age, abdominal pain or discomfort, allergic history and direct bilirubin were associated with pLNM, which may be helpful for preoperative selection.

Statistical Optimization of Medium Components for Milk-Clotting Enzyme Production by Bacillus amyloliquefaciens D4 Using Wheat Bran-an Agro-Industry Waste

( Wei Bing Zhang ),( Xiao Ling He ),( Hong Na Liu ),( Hui Yuan Guo ),( Fa Zheng Ren ),( Wei Dong Gao ),( Peng Cheng Wen ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.8

In this paper, two statistical methods were applied to optimize medium components to improve the production of the milk-clotting enzyme by Bacillus amyloliquefaciens D4. First, wheat bran juice, skim milk powder, and Na2HPO4 were shown to have significant effects on D4 enzyme production using the Plackett?Burman experimental design. Subsequently, an optimal medium was obtained using the Box?Behnken method, which consisted of 3.31 g/l of skim milk powder, 5.0 g/l of sucrose, 0.1 g/l of FeSO4?7H2O, 0.1 g/l of MgSO4?7H2O, 0.1 g/l of MnSO4?2H2O, 0.1 g/l of ZnSO4?7H2O, 1.52 g/l of Na2HPO4, and 172.45 g/l of wheat bran juice. With this optimal medium, the milk-clotting enzyme production was remarkably enhanced. The milk-clotting enzyme activity reached 3,326.7 SU/ml after incubation of 48 h, which was 1.76-fold higher than that of the basic medium, showing that the Plackett?Burman design and Box?Behnken response surface method are effective to optimize medium components, and B. amyloliquefaciens D4 possessed a high rennet-producing capacity in the optimal medium.

Study on Thermodynamics of Three Kinds of Benzindocarbocyanine Dyes in Aqueous Methanol Solution

Wei Huang,Lan-Ying Wang,Yi-Le Fu,Ji-Quan Liu,You-Ni Tao,Fang-Li Fan,Gao-Hong Zhai,Zhen-Yi Wen 대한화학회 2009 Bulletin of the Korean Chemical Society Vol.30 No.3

Aggregation behavior of three kinds of benzindocarbocyanine dyes in aqueous methanol solution was studied by UV-Vis absorption spectrum. The results indicated that the three dyes all existed monomer-dimer equilibrium in aqueous methanol solution (concentration range 10−5 to 10−6 M) at 25.0~41.0 °C for Dye 1, 28.0~49.0 oC for Dye 2 and 26.0~47.0 °C for Dye 3. The fundamental property of the three dyes as the dimeric association constant KD, the dimeric free energy ΔGD, the dimeric entropy ΔSD, and the dimeric enthalpy ΔHD were determined. The ΔHD of three dyes: Dye 1, Dye 2 and Dye 3 was -42.5, -15.1 and -18.9 kJ/mol, respectively. The experimental observations were the subject of a theoretical study including the ground-state geometries which were fully optimized using DFT at B3LYP/6-31G level. The effect of dye molecule structure on ΔHD was discussed by theoretical calculations.

Analysis on the safe distance between shield tunnel through sand stratum and underlying karst cave

Wei Wan,Shiming Gao,Lingfeng Liu,Wushuang Wen,Ping Li,Jianping Chen 한국자원공학회 2019 Geosystem engineering Vol.22 No.2

When a subway tunnel passes through a karst geological area, in order to ensure the stability of the tunnel, the safe distance between the tunnel and karst cave needs to be researched. This article is based on the construction project of Wuhan metro line 6 from Qianjincun station to Mayinglu station. In view of the geological condition of overlying sand and underlying limestone, by using numerical simulation and theoretical calculation and selecting a characteristic section, the influence law and degree of karst cave span, height-span ratio, and filling degree on the safe thickness of karst cave roof are studied and a forecasting model of the safe thickness of karst cave roof is established. Combined with dichotomy and numerical simulation, the critical horizontal safe distance beyond which cave collapse does not cause tunnel instability is calculated. The results show that: (1) the safe thickness of karst cave roof increases linearly with the increase of karst cave span, and decreases with the increase of height-span ratio and filling degree; (2) among the three factors, the effect of karst cave span is the most significant, the filling degree of karst cave is the second, and the influence of height-span ratio of karst cave is the weakest; (3) the proposed forecasting model passes the significance test, indicating that the model is very reasonable; and (4) the critical horizontal safe distance from the tunnel border to the karst cave boundary is 22.4 m. The research results can provide guidance for the design and construction of shield tunnels and karst cave treatment in karst areas.

Deep-blue organic light-emitting diodes based on multi-tert-butyl modified naphthylene

Bin Wei,Hanfei Xu,Ning Zhao,Xicun Gao,Yiteng Ye,Yingnan Wang,Dengli Yao,Xiao-Wen Zhang,Xiujuan Liu 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.102 No.-

Six tert-butyls modified naphthyl derivative (2,6-di-tert-butyl-1,5-bis-(3,5-di-tert-butyl-phenyl)-naphthalene (N(dtb)4) was synthesized and tested for host and guest materials in organic light-emittingdiodes (OLEDs). Blue OLED using N(dtb)4 as a host and an emitter DSA-PH as a guest reaches an efficiencyof 11.52 cd A 1 (maximum external quantum efficiency 7.8 % and maximum power efficiency10.33 lm W 1). In addition, for the OLED with N(dtb)4 being a guest, we find that the concentrations ofN(dtb)4 will also affect the performance of the OLED, among which ADN: 5 wt.% N(dtb)4 has higher efficiency,and the maximum current efficiency, external quantum efficiency (EQE) and power efficiency ofthe device reach 3.75 cd A 1, 3.93 % and 3.36 lm W 1, respectively. We also investigated the pure N(dtb)4-based ultraviolet OLED (UV-OLED), and achieved the maximum EQE of the device is 1.12 %. These resultsindicate that N(dtb)4, a novel synthesized fluorescent organic material, has great potential applications inOLEDs and accordingly advancing lightings and displays.

Whole Brain Radiotherapy Plus Chemotherapy in the Treatment of Brain Metastases from Lung Cancer: A Meta-analysis of 19 Randomized Controlled Trails

Liu, Wen-Jing,Zeng, Xian-Tao,Qin, Hai-Feng,Gao, Hong-Jun,Bi, Wei-Jing,Liu, Xiao-Qing Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.7

Objective: To evaluate the efficacy and safety of whole brain radiotherapy (WBRT) plus chemotherapy versus WBRT alone for treating brain metastases (BM) from lung cancer by performing a meta-analysis based on randomized controlled trials (RCTs). Methods: The PubMed, Embase, CENTRAL, ASCO, ESMO, CBM, CNKI, and VIP databases were searched for relevant RCTs performed between January 2000 and March 2012. After quality assessment and data extraction, the meta-analysis was performed using the RevMan 5.1 software, with funnel plot evaluation of publication bias. Results: 19 RCTs involving 1,343 patients were included. The meta-analyses demonstrated that compared to WBRT alone, WBRT plus chemotherapy was more effective with regard to the objective response rate (OR = 2.30, 95% CI = 1.79 - 2.98; P < 0.001); however, the incidences of gastrointestinal reactions (RR = 3.82, 95% CI = 2.33 - 6.28, P <0.001), bone marrow suppression (RR = 5.49, 95% CI = 3.65 - 8.25, P < 0.001), thrombocytopenia (RR = 5.83, 95% CI = 0.39 - 86.59; P = 0.20), leukopenia (RR = 3.13, 95% CI = 1.77 - 5.51; P < 0.001), and neutropenia (RR = 2.75, 95% CI = 1.61 - 4.68; P < 0.001) in patients treated with WBRT plus chemotherapy were higher than with WBRT alone. There was no obvious publication bias detected. Conclusion: WBRT plus chemotherapy can obviously improve total efficacy rate, butalso increases the incidence of adverse reactions compared to WBRT alone. From the limitations of this study, more large-scale, high-quality RCTs are suggested for further verification.

Protective Effect of Astragalus polysaccharides on Liver Injury Induced by Several Different Chemotherapeutics in Mice

Liu, Wen,Gao, Fang-Fang,Li, Qun,Lv, Jia-Wei,Wang, Ying,Hu, Peng-Chao,Xiang, Qing-Ming,Wei, Lei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.23

Side effects are an unavoidable consequence of chemotherapy drugs, during which liver injury often takes place. The current study was designed to investigate the protective effect of Astragalus polysaccharides (APS) against the hepatotoxicity induced by frequently-used chemical therapy agents, cyclophosphamide (CTX), docetaxel (DTX) and epirubicin (EPI)) in mice. Mice were divided into five groups, controls, low or high dose groups ($DTX_L$, $CTX_L$, $EPI_L$ or $DTX_H$, $CTX_H$, $EPI_H$), and low or high dose chemotherapeutics+APS groups ($DTX_L$+APS, $CTX_L$+APS, $EPI_L$+APS or $DTX_H$+APS, $CTX_H$+APS, $EPI_H$+APS). Controls were treated with equivalent normal saline for 28 days every other day; low or high dose group were intraperitoneal (i.p) injected with low or high doses of CTX, DTX and EPI for 28 days every other day; low or high dose chemotherapeutics+APS group were separately intraperitoneal (i.p) injected with chemotherapeutics for 28 days every other day and i.p with APS (100 mg/kg) for 7 days continually from the 22th to the 28th days. The body weight, serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), histopathological features, and ultrastructure morphological change of liver tissues, protein expression level of caspase-3 were estimated at different time points. With high dose treatment of DTX, CTX and EPI, weight gain was inhibited and serum levels of ALT and AST were significantly increased. Sections of liver tissue showed massive hepatotoxicity in $CTX_H$ group compared to the control group, including hepatic lobule disorder, granular and vacuolar degeneration and necrosis in hepatic cells. These changes were confirmed at ultrastructural level, including obvious pyknosis, heterochromatin aggregation, nuclear membrane resolution, and chondrosome crystal decrease. Western blotting revealed that the protein levels of caspase-3 increased in $CTX_H$ group. The low dose groups exhibited trivial hepatotoxicity. More interestingly, after 100 mg/kg APS, liver injury was redecued not only regarding serum transaminase activities (low or high dose chemotherapeutics+APS group), but also from pathological and ultrastructural changes and the protein levels of caspase-3 ($CTX_H$+APS group). In conclusion, DTX, CTX and EPI induce liver damage in a dose dependent manner, whereas APS exerted protective effects.

Numerical analysis of a dual-wave band guided-mode resonance filter with embedded bilayer asymmetric metallic gratings

Ma Wen-Zhuang,Liu Jing,Yu Gao-Xiang,Chen Wei,Chen Yu-Shan,Deng Xu-Chu 한국물리학회 2021 Current Applied Physics Vol.27 No.-

In this paper, a novel guided-mode resonances (GMRs) based embedded dual-wavelength filter, which had the bilayer gratings located at the two sides of dielectric substrate and arranged at asymmetric position, was investigated and studied theoretically. As compared to symmetric structures that usually provided single wavelength of transmission resonance, as one normal incidence was used for transverse magnetic polarized light, the designed asymmetric structure could generate two remarkable narrow band wavelengths of transmission resonance. The parameters to affect the resonance wavelengths and the transmission resonance Q value (Q = λ/Δλ) of two bands were the distance between the two metallic gratings, the relatively lateral positions of the upper and lower gratings, the number of structure period, the thicknesses of metallic gratings, and the thickness of dielectric film. By optimizing the designed parameters, a GMR device with two resonance wavelengths located at 1239 and 1302 nm showed relative optimal performance because they had high transmission depth (99.9% and 90%) and ultra-narrow transmission bandwidth (2.8 and 1.8 nm) at the two resonance wavelengths. The presented structure can offer a potential route towards dual-band narrow-band filters and refractive index sensors in the near infrared.

Study on Thermodynamics of Three Kinds of Benzindocarbocyanine Dyes in Aqueous Methanol Solution

Huang, Wei,Wang, Lan-Ying,Fu, Yi-Le,Liu, Ji-Quan,Tao, You-Ni,Fan, Fang-Li,Zhai, Gao-Hong,Wen, Zhen-Yi Korean Chemical Society 2009 Bulletin of the Korean Chemical Society Vol.30 No.3

Aggregation behavior of three kinds of benzindocarbocyanine dyes in aqueous methanol solution was studied by UV-Vis absorption spectrum. The results indicated that the three dyes all existed monomer-dimer equilibrium in aqueous methanol solution (concentration range $10^{−5}\;to\;10^{−6}$ M) at 25.0$\sim$41.0 ${^{\circ}C}$ for Dye 1, 28.0$\sim$49.0 ${^{\circ}C}$ for Dye 2 and 26.0$\sim$47.0 ${^{\circ}C}$ for Dye 3. The fundamental property of the three dyes as the dimeric association constant KD, the dimeric free energy ${\Delta$}G_D, the dimeric entropy ${\Delta$}S_D, and the dimeric enthalpy ${\Delta$}H_D were determined. The ${\Delta$}H_D of three dyes: Dye 1, Dye 2 and Dye 3 was -42.5, -15.1 and -18.9 kJ/mol, respectively. The experimental observations were the subject of a theoretical study including the ground-state geometries which were fully optimized using DFT at B3LYP/6-31G level. The effect of dye molecule structure on ${\Delta$}H_D was discussed by theoretical calculations.

Endothelin-1 enhances the melanogenesis via MITF-GPNMB pathway

Ping Zhang,Wei Liu,Xiaoying Yuan,Dong Guang Li,We Ijie Gu,Tian Wen Gao 생화학분자생물학회(구 한국생화학분자생물학회) 2013 BMB Reports Vol.46 No.7