Characterization and Antioxidant Activity of the Exopolysaccharide Produced by Bacillus amyloliquefaciens GSBa-1

( Wen Zhao ),( Jian Zhang ),( Yun-yun Jiang ),( Xiao Zhao ),( Xiao-na Hao ),( Liu Li ),( Zhen-nai Yang ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.8

The exopolysaccharide (EPS) produced by Bacillus amyloliquefaciens GSBa-1 was isolated and purified by ethanol precipitation, and DEAE-cellulose and Sepharose CL-6B chromatographies. The molecular mass of the purified EPS was determined to be 54 kDa. Monosaccharide analysis showed that the EPS was composed of predominantly glucose, and it was further confirmed by NMR spectroscopy to be α-glucan that consisted of a trisaccharide repeating unit with possible presence of two α-(1→3) and one α-(1→6) glucosidic linkages. Microstructural analysis showed that the EPS appeared as ellipsoid or globose with a smooth surface. The EPS had a degradation temperature at 240℃. Furthermore, the EPS had strong DPPH and hydroxyl radical scavenging activities, and moderate superoxidant anion scavenging and metal ion-chelating activities. This is the first characterization of a glucan produced by B. amyloliquefaciens with strong antioxidant activity. The results of this study suggest the potential of the EPS from B. amyloliquefaciens GSBa-1 to serve as a natural antioxidant for application in functional products.

Effect of sodium sulfite, tartaric acid, tannin, and glucose on rheological properties, release of aroma compounds, and color characteristics of red wine

Hao Wang,Zhi-Jing Ni,Wen-Ping Ma,Chang-Bing Song,Jian-Guo Zhang,Kiran Thakur,Zhao-Jun Wei 한국식품과학회 2019 Food Science and Biotechnology Vol.28 No.2

In this study, we evaluated the effect of additionof non-volatile compounds (sodium sulfite, tartaric acid,tannin, and glucose) on the rheological properties, releaseof aroma compounds, and color of the red wine. Whiledetermining the rheological properties of the supplementedsamples, non-Newtonian fluidic and shear-thinningbehavior of samples was noticed. The viscosity of thesesamples was found in negative correlation with the dose ofaddition of various non-volatile substances. The aromaprofile of red wine after additions showed the change in therelease of the nine key aroma compounds. Among themethyl hexanoate, phenylethyl alcohol, octanoic acid, diethylsuccinate, and ethyl octanoate were profoundly increased. Further, the color of red wines was improved in the presenceof tartaric acid and tannin. Overall, supplementationof various substances during storage period of three differentwines could enormously affect the sensory characteristicsin a dose dependent manner.

Analytical study on free vertical and torsional vibrations of two- and threepylon suspension bridges via d’Alembert’s principle

Wen-Ming Zhang,Zhi-wei Wang,Hao-qing Zhang,Xiao-fan Lu,Zhao Liu 국제구조공학회 2020 Structural Engineering and Mechanics, An Int'l Jou Vol.76 No.3

This study derives the differential equations of free vertical bending and torsional vibrations for two- and three-pylon suspension bridges using d'Alembert's principle. The respective algorithms for natural vibration frequency and vibration mode are established through the separation of variables. In the case of the three-pylon suspension bridge, the effect of the along-bridge bending vibration of the middle pylon on the vertical bending vibration of the entire bridge is considered. The impact of torsional vibration of the middle pylon about the vertical axis on the torsional vibration of the entire bridge is also analyzed in detail. The feasibility of the proposed method is verified by two engineering examples. A comparative analysis of the results obtained via the proposed and more intricate finite element methods confirmed the former feasibility. Finally, the middle pylon stiffness effect on the vibration frequency of the three-pylon suspension bridge is discussed. It is found that the vibration frequencies of the first- and thirdorder vertical bending and torsional modes both increase with the middle pylon stiffness. However, the increase amplitudes of thirdorder bending and torsional modes are relatively small with the middle pylon stiffness increase. Moreover, the second-order bending and torsional frequencies do not change with the middle pylon stiffness.

Application of Complex Probiotics in Swine Nutrition - A Review

Liu, Wen Chao,Ye, Mao,Liao, Jia Hao,Zhao, Zhi Hui,Kim, In Ho,An, Li Long De Gruyter Open 2018 Annals of animal science Vol.18 No.2

<P>The use of probiotics as alternatives to antibiotics for farm animals is gaining more and more interest during recent years. Probiotics are living microorganisms that provide a wide variety of health benefits to the host when ingested in adequate amounts. The bacterial strains most frequently used as probiotic agents are Bacillus, lactic acid bacteria, Enterococcus and Saccharomyces cerevisiae. It has been suggested that multi-strain probiotics might be more effective than mono-strain probiotics due to the additive and synergistic effects, and many previous studies demonstrated that dietary complex probiotics supplementation had growth promoting effects on pigs. However, the effect of complex probiotics in practice is not always consistent, the effect of probiotic could be affected by strain composition, dosage, feed formula, and the age of animals. In this review, we will give an overview on the current use of complex probiotics for weaning, growing and finishing pigs and sows.</P>

Combination Doxorubicin and Interferon-α Therapy Stimulates Immunogenicity of Murine Pancreatic Cancer Panc02 Cells via Up-regulation of NKG2D ligands and MHC Class I

Wang, Wen-Jia,Qin, Si-Hao,Zhang, Ji-Wei,Jiang, Yue-Yao,Zhang, Jin-Nan,Zhao, Lei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.22

Background: Pancreatic adenocarcinoma is a malignant gastrointestinal cancer with significant morbidity and mortality. Despite severe side effects of chemotherapy, the use of immunotherapy combined with chemotherapy has emerged as a common clinical treatment. In this study, we investigated the efficacy of the combined doxorubicin and interferon-${\alpha}$ (IFN-${\alpha}$) therapy on murine pancreatic cancer Panc02 cells in vitro and in vivo and underlying mechanisms. Materials and Methods: A Panc02-bearing mouse model was established to determine whether doxorubicin and interferon-${\alpha}$ (IFN-${\alpha}$) could effectively inhibit tumor growth in vivo. Cytotoxicity of natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) was evaluated using a standard LDH release assay. To evaluate the relevance of NK cells and CD8 T cells to the combination therapy-mediated anti-tumor effects, they were depleted in tumor-bearing mice by injecting anti-asialo-GM-1 antibodies or anti-CD8 antibodies, respectively. Finally, the influence of doxorubicin+interferon-${\alpha}$ (IFN-${\alpha}$) on the ligands of NK and T cells was assessed by flow cytometry. Results: The combination therapy group demonstrated a significant inhibition of growth of Panc02 in vivo, resulting from activated cytotoxicity of NK cells and CTLs. Depleting CD8 T cells or NK cells reduced the anticancer effects mediated by immunochemotherapy. Furthermore, the doxorubicin+IFN-a treatment increased the expression of major histocompatibility complex class I (MHC I) and NKG2D ligands on Panc02 cells, suggesting that the combined therapy may be a potential strategy for enhancing immunogenicity of tumors. All these data indicate that the combination therapy using doxorubicin and interferon-${\alpha}$ (IFN-${\alpha}$) may be a potential strategy for treating pancreatic adenocarcinoma.

Aberrant Expression of Pim-3 Promotes Proliferation and Migration of Ovarian Cancer Cells

Zhuang, Hao,Zhao, Man-Yin,Hei, Kai-Wen,Yang, Bai-Cai,Sun, Li,Du, Xue,Li, Yong-Mei Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.8

Pim kinase-3(Pim-3), a member of serine/threonine protein kinases, has been implicated in multiple human cancers and involved in Myc-induced tumorigenesis. However, little is known regarding its expression and biological function in human ovarian cancer. In this study we showed that the clinical significance and biological functions of Pim-3 in ovarian cancer and found that higher Pim-3 mRNA level are detected in ovarian cancer tissues than those in normal ovarian tissues. There are significant correlations between higher Pim-3 expression levels with the FIGO stage, histopathological subtypes, and distant metastasis in ovarian cancer patients. Lentivirus-mediated gene overexpression of Pim-3 significantly promotes the proliferation and migration of SKOV3 cell lines. Furthermore, MACC1 and Pim-3 expression were significantly correlated in human ovarian cancer cells, and overexpression of Pim-3 in ovary cancer cells increased MACC1 mRNA and protein expression. The data indicate that Pim-3 acts as a putative oncogene in ovary cancer and could be a viable diagnostic and therapeutic target for ovarian cancer.

Exercise Reduces Airway Smooth Muscle Contraction in Asthmatic Rats via Inhibition of IL-4 Secretion and Store-Operated Ca2+ Entry Pathway

Huang Jun-Hao,Gao Hui-Wen,Gao Dong-Dong,Yang Wei-Yue,Zhao Meng-Ke,Shen Bing,Hu Min 대한천식알레르기학회 2023 Allergy, Asthma & Immunology Research Vol.15 No.3

Purpose: Increased evidence has shown that aerobic exercise reduces airway hyperresponsiveness in asthmatic individuals. However, the underlying mechanisms of action remain elusive. This study aimed to investigate the effect of exercise on airway smooth muscle (ASM) contractile function in asthmatic rats, and uncover the possible involvement of interleukin 4 (IL-4) and the store-operated Ca2+ entry (SOCE) pathway. Methods: In this study, chicken ovalbumin was used to induce asthma in male Sprague-Dawley rats. The exercise group received moderate-intensity aerobic exercise training for 4 weeks. IL-4 concentrations in bronchoalveolar lavage fluid (BALF) samples were evaluated by enzyme linked immunosorbent assay. The contractile function of the ASM was investigated using tracheal ring tension experiments and intracellular Ca2+ imaging techniques. Western blot analysis was used to evaluate expression levels of calcium-release activated calcium (CRAC) channel protein (Orai) and stromal interaction molecule 1 (STIM1) in ASM. Results: Our data showed that the carbachol-stimulated, SOCE-mediated contraction of rat ASM was significantly increased in asthmatic rats, which could be abolished by exercise. Pharmacological studies revealed that GSK5498A and BTP-2, selective blockers of CRAC channels significantly inhibited SOCE-induced ASM contraction. In addition, exercise inhibited the up-regulation of IL-4 in BALF as well as STIM1 and Orai expression in the ASM of asthmatic rats. In line with these observations, we demonstrated that pretreatment of the ASM with IL-4 up-regulated the expression level of STIM1, Orai1 and Orai2, thereby promoting SOCE-mediated ASM contraction. Conclusions: The data in this study reveal that aerobic exercise may improve the ASM contractile function in asthmatic rats by inhibiting IL-4 secretion and by down-regulating the expression of STIM1, Orai1 and Orai2, thus decreasing excessive SOCE-mediated ASM contraction in asthmatic rats.

Syntheses, Structure, and Properties of Four Metal-Organic Polymers Based on Rigid Multiple Carboxylate Ligands and N-Donor Ligands

Yong Zhou,Wen-Hao Zhao,Jin Wang,Jin-Rui Wei,Xian-Hong Yin,Xiang-Bo Wei,Cui-Wu Lin 대한화학회 2015 Bulletin of the Korean Chemical Society Vol.36 No.11

Four metal-organic coordination polymers, {[Cd2(BIBP)2(H2DTDA)2]n  · 2(H2O)} (1), [Zn (BIBP) (H2TDA)2] n (2), [Cd2(BIBP)(H2TDA)2] n (3), and [Ni2(BIBP)2(H2DTDA)2(μ-O)] n , (4) [where BIBP = 4,4′-bis(1-imidazoly)biphenyl, H2TDA = [1,1′:4′1″-terphen-yl]-3,3″-dicarboxylic acid, and H2DTDA = 2′,5′-dimethyl-[1,1′:4′,1″-terphenyl]-3,3″-dicarboxylic acid] were hydrothermally synthesized and characterized by elemental analyses, IR, TG, luminescence, and single crystal X-ray diffraction. X-Ray diffraction analysis reveals that the four complexes exhibit new frameworks due to diverse coordination conformations. The different coordination modes of the ligands BIBP and two aromatic carboxylate acids play important roles in the construction of the final structure for the complexes. X-ray structure analysis reveals that 1, 2, and 4 are 3D coordination polymers, while complex 3 is a 2D network polymer.

Hsa-miRNA-143-3p Reverses Multidrug Resistance of Triple-Negative Breast Cancer by Inhibiting the Expression of Its Target Protein Cytokine-Induced Apoptosis Inhibitor 1 In Vivo

Yu Wei Deng,Wen Jing Hao,Yi Wen Li,Yi Xin Li,Bo Chen Zhao,Dan Lu 한국유방암학회 2018 Journal of breast cancer Vol.21 No.3

Purpose: Multidrug resistance (MDR) remains a major obstacle in the treatment of triple-negative breast cancer (TNBC) with conventional chemotherapeutic agents. A previous study demonstrated that hsa-miRNA-143-3p plays a vital role in drug resistance of TNBC. Downregulation of hsa-miRNA-143-3p upregulated the expression of its target protein cytokine-induced apoptosis inhibitor 1 (CIAPIN1) in order to activate MDR, while upregulation of hsa-miRNA-143-3p effectively enhances the sensitivity of drug-resistant TNBC cells to chemotherapeutics. The present study aimed to further verify these findings in vivo. Methods: We established a hypodermic tumor nude mice model using paclitaxel- resistant TNBC cells. We expressed ectopic hsa-miRNA- 143-3p under the control of a breast cancer-specific human mammaglobin promoter that guided the efficient expression of exogenous hsa-miRNA-143-3p only in breast cancer cells. Thereafter, we overexpressed hsa-miRNA-143-3p in xenografts using a recombinant virus system and quantified the expression of hsa-miRNA-143-3p, CIAPIN1 protein, and proteins encoded by related functional genes by western blot. Results: We successfully completed the prospective exploration of the intravenous virus injection pattern from extensive expression to targeted expression. The overexpression of hsa-miRNA-143-3p significantly alleviated chemoresistance of TNBC by inhibiting viability. In addition, we observed that the expression of CIAPIN1 as a hsa-miRNA-143-3p target protein was remarkably decreased. Conclusion: We partly illustrated the mechanism underlying the hsa-miRNA-143-3p/CIAPIN1 drug resistance pathway. HsamiRNA- 143-3p as a tumor suppressive microRNA may be a novel target to effectively reverse MDR of TNBC in vivo.

Vibration control of rotor system by ultrasonic excited limiter

Hongliang Yao,Qian Zhao,Hao Wu,Bang-chun Wen 대한기계학회 2015 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.29 No.5

We propose a novel method with an ultrasonically excited structure as a vibration limiter to control the vibration of rotor systems. Thefriction between the rotor and the limiter is significantly reduced by ultrasonic lubrication, thereby preventing the occurrence of rubbinginducedthermal bowing, which is commonly observed in traditional vibration limiters. In our study, the friction reduction mechanism ofthe ultrasonically excited vibration limiter was first examined based on tribology theory and Hertz contact theory. The vibration controleffects and the alleviation of thermal bowing were then investigated by numerical simulation. Finally, vibration control experiments wereperformed on a Bentley test rig with the ultrasonically excited vibration limiter. The consistency between theoretical and experimentalresults confirmed the effectiveness of the proposed method.