Reusable, magnetic laser-induced graphene for efficient removal of organic pollutants from water

Jiang Ye,Wan Sijie,Zhao Weiwei,Yu Wenjie,Wang Shuaipeng,Yu Zeqi,Yang Qiu,Zhou Weihua,Liu Xiaoqing 한국탄소학회 2022 Carbon Letters Vol.32 No.4

The hybridization of graphene with magnetic nanoparticles has endowed graphene with increasing interest as the adsorbent for wastewater treatment. However, its fabrication often involves a multi-stepped chemical synthesis process. In this work, we demonstrate a facile, one-step, and solvent-free approach to fabricate Fe3O4 nanoparticle-anchored Laser-Induced Graphene (Fe3O4@LIG) as an efficient adsorbent by direct laser irradiation on a ferric acetylacetonate containing polybenzoxazine film. Raman and X-ray diffraction analysis confirm the graphene component in the adsorbent, and the morphology characterizations show that Fe3O4 nanoparticles are distributed uniformly on LIG with hierarchical meso- and macro-porous structures. Adsorption experiments indicate that Fe3O4@LIG can adsorb methylene blue (MB) from aqueous solutions in a fast and effective manner, with a maximum adsorption capacity up to 350.9 mg/g. The adsorption kinetics and isotherms are also investigated, which are well-described by the pseudo-second-order model and Langmuir model, respectively. Additionally, Fe3O4@LIG is also demonstrated with the efficient removal of a variety of organic solvents from water. The favorable adsorption behavior of Fe3O4@LIG is attributed to its unique porous structure and the molecular interactions with adsorbates. On the other hand, Fe3O4@LIG has high magnetic property, and therefore, it could be easily recovered from water and well regenerated for repeated use. With the efficient adsorption of organic pollutants, magnetic separability, and good recyclability, it is believed that the easy-fabricated Fe3O4@LIG has great potential applications in wastewater treatment.

MiR-1297 and MiR-26a-5p Inhibit Cell Progression of Keratinocytes in Cholesteatoma Depending on the Regulation of BMI1

Xiaodan Zhu,Fanglei Ye,Shaojuan Hao,Qiuning Yu,Yang Wang,Weihua Lou,Kun Zhao,Hongmin Li 한국생물공학회 2022 Biotechnology and Bioprocess Engineering Vol.27 No.1

Cholesteatoma is a pathologically benign but clinically destructive middle ear disease characterized by hyperproliferative keratinocytes. B-cell-specific Moloney murine leukemia virus insertion site 1 (BMI1) has been reported to be upregulated in cholesteatoma tissues. This study aimed to explore the biological role and underlying mechanisms of BMI1 in the progression of cholesteatoma. The expression levels of microRNA (miR)-1297, miR-26a- 5p, and BMI1 in cholesteatoma tissues and cells were examined by quantitative real-time polymerase chain reaction (qRT-PCR) or western blot. Functional experiments were performed by CCK-8 assay for cell proliferation viability, 5-ethynyl-2'deoxyuridine (EdU) incorporation assay for DNA biosynthesis, colony formation assay for cloning forming ability analysis, transwell assay and wound healing assay for cell metastasis, flow cytometry for cell cycle distribution and cell apoptosis. The protein expression of apoptosis-associated proteins was investigated by western blot. Dual-luciferase reporter assay was conducted to verify the interaction between miR-1297 or miR-26a-5p and BMI1. BMI1 was highly expressed in cholesteatoma tumor tissues. Functional analyses showed that BMI1 knockdown could inhibit the proliferation, colony formation, migration, invasion, cell cycle progression and promoted the apoptosis of keratinocytes. Mechanically, BMI1 was a target of miR-1297 and miR-26a-5p. Moreover, the rescue experiments presented that BMI1 addition could abolish the suppressive effects of miR-1297 or miR-26a-5p overexpression on cell malignant behaviors in keratinocytes. BMI1 could exert an oncogenic role in the malignant development of cholesteatoma through serving as the targets of miR-1297 and miR-26a-5p, which might provide novel strategies for cholesteatoma treatment.

Optimizations of Multi-hop Cooperative Molecular Communication in Cylindrical Anomalous-Diffusive Channel

Xuancheng Jin,Zhen Cheng,Zhian Ye,Weihua Gong 한국인터넷정보학회 2024 KSII Transactions on Internet and Information Syst Vol.18 No.4

In this paper, the optimizations of multi-hop cooperative molecular communication (CMC) system in cylindrical anomalous-diffusive channel in three-dimensional enviroment are investigated. First, we derive the performance of bit error probability (BEP) of CMC system under decode-and-forward relay strategy. Then for achieving minimum average BEP, the optimization variables are detection thresholds at cooperative nodes and destination node, and the corresponding optimization problem is formulated. Furthermore, we use conjugate gradient (CG) algorithm to solve this optimization problem to search optimal detection thresholds. The numerical results show the optimal detection thresholds can be obtained by CG algorithm, which has good convergence behaviors with fewer iterations to achieve minimized average BEP compared with gradient decent algorithm and Bisection method which are used in molecular communication.

Effect of Overfeeding on Plasma Parameters and mRNA Expression of Genes Associated with Hepatic Lipogenesis in Geese

Han, Chunchun,Wang, Jiwen,Xu, Hengyong,Li, Liang,Ye, Jianqiang,Li, Jiang,Zhuo, Weihua Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.4

The aim of our study was to research the effect of overfeeding on plasma parameters and mRNA expression of genes associated with hepatic lipogenesis in the Sichuan white goose and Landes goose. Fifty-four male Landes geese and 57 male Sichuan white geese were hatched on the same day under the same feeding conditions. After overfeeding for 14 days, (1) extrahepatic adipose tissues grew greatly in the Sichuan white geese, while more lipid accumulated in liver tissue in the Landes geese. (2) Sichuan white geese had a higher plasma concentration of triacylglycerols (TG), lipoproteins and insulin than the Landes geese. However, the Landes geese exhibited higher increase of plasma concentrations of TG, lipoproteins and insulin, with greater decrease of the diacylglycerol acyltransferase 2 (DGAT2) activity and DGAT2 mRNA level and a smller decrease of plasma glucose concentration. In addition, the mRNA level of MTP and LPL in liver was down- and up- regulated by overfeeding, respectively. (3) The correlations between the activity of LPL and the proportions of subcutaneous adipose tissue, abdominal adipose tissue, and liver weight, and the plasma concentration of VLDL were different in the two breeds. (4) The proportion of fatty liver weight was positively correlated to plasma concentrations of VLDL and TG in the overfed Sichuan white geese. Such a relationship did not exist in the Landes geese. (5) The activity of DGAT2 and its mRNA abundance in liver had significant negative correlations with the TG content in liver lipid and plasma insulin level in the Landes geese, while in the Sichuan white geese they had negative correlation (p>0.05) with TG concentration in liver lipid and had significant positive correlation with VLDL and TG concentrations in plasma.

Studies on seismic performance of the new section steel beam-wall connection joint

Weicheng Su,Jian Liu,Changjiang Liu,Chiyu Luo,Weihua Ye,Yaojun Deng 국제구조공학회 2023 Structural Engineering and Mechanics, An Int'l Jou Vol.88 No.5

This paper introduces a new hybrid structural connection joint that combines shear walls with section steel beams, fundamentally resolving the construction complexity issue of requiring pre-embedded connectors in the connection between shear walls and steel beams. Initially, a quasi-static loading scheme with load-deformation dual control was employed to conduct low-cycle repeated loading experiments on five new connection joints. Data was acquired using displacement and strain gauges to compare the energy dissipation coefficients of each specimen. The destruction process of the new connection joints was meticulously observed and recorded, delineating it into three stages. Hysteresis curves and skeleton curves of the joint specimens were plotted based on experimental results, summarizing the energy dissipation performance of the joints. It's noteworthy that the addition of shear walls led to an approximate 17% increase in the energy dissipation coefficient. The energy dissipation coefficients of dog-bone-shaped connection joints with shear walls and cover plates reached 2.043 and 2.059, respectively, exhibiting the most comprehensive hysteresis curves. Additionally, the impact of laminated steel plates covering composite concrete floors on the stiffness of semi-rigid joint ends under excessive stretching should not be disregarded. A comparison with finite element analysis results yielded an error of merely 2.2%, offering substantial evidence for the wide-ranging application prospects of this innovative joint in seismic performance.

Estimation of Lipoprotein-lipase Activity (LPL) and Other Biochemical Changes in Two Breeds of Overfeeding Geese

Xu, Hengyong,Wang, Yan,Han, Chunchun,Jiang, Li,Zhuo, Weihua,Ye, Jianqiang,Wang, Jiwen Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.9

This study aimed to examine the effect of overfeeding on biochemical parameters and lipoprotein-lipase (LPL) mRNA expression in different tissues associated with hepatic lipogenesis in Sichuan white and Landes geese. Fifty healthy male Sichuan white geese and fifty male Landes geese (Cygnus atratus) were hatched on the same day under the same feeding conditions and were selected as experimental animals. After overfeeding for 14 days (from 14 weeks to 16 weeks) and then slaughtering, the biochemical changes of hepatic lipogenesis were evaluated. Results showed that i) in Landes geese, the plasma concentration of glucose was higher (p<0.001), while plasma concentrations of insulin and VLDL were both lower (p<0.01); ii) the LPL mRNA level in pectoralis muscle and leg muscle of the overfed groups in both breeds was higher (p<0.05) than in the control groups; iii) in Sichuan white geese, the proportion of fatty liver weight was positively correlated with plasma triacylglycerols (TG)(p<0.05) and VLDL concentrations (p<0.05), while these correlations were not significant in Landes geese; and iv) the activity of LPL had significant positive correlation with the proportions of lipids in subcutaneous adipose tissue and abdominal adipose tissue in Sichuan white geese, while in Landes geese the correlation was negative (p<0.05) with proportions of lipids in the liver, LPL activity had a significant positive correlation with the proportions of lipids in subcutaneous adipose tissue. These results suggest that the Landes geese have a better ability to use the massive amount of ingested food and to store lipids preferentially in the liver, but the Sichuan white geese have a relatively lower ability to use energetic nutrients and lipid storage is more efficient in the adipose tissues.