Mesenchymal-to-epithelial transition of osteoblasts induced by Fam20c knockout

Geng Ya-Wei,Zhang Zhen,Jin Han,Da Jun-Long,Zhang Kai,Wang Jian-Qun,Guo Yu-Yao,Zhang Bin,Li Ying 한국유전학회 2022 Genes & Genomics Vol.44 No.2

Background: Fam20c is intimately related to tissue development and diseases. At present, it has been reported that Fam20c regulates the mineralization of osteoblasts, but there are few reports on other effects. Objective: To study the effect of Fam20c on osteoblasts by knocking out the Fam20c gene. Methods: Fam20c knockout osteoblasts were constructed by transfecting mouse osteoblasts with lentivirus. The proliferation, migration and mineralization of Fam20c knockout cells were detected by CCK-8, scratch test and alizarin red staining assays. The subcellular structure was observed by transmission electron microscopy. RT-PCR was used to detect the differential expression of mesenchymal-to-epithelial transition (MET)-related marker genes and core transcription factors. The differential expression of MET-related proteins was detected by immunofluorescence or Western blot. Transcriptome analysis of Fam20c knockout osteoblasts was performed, and real-time PCR was used to verify transcriptome analysis related to MET. Results: The proliferation ability of osteoblasts was not significantly changed after Fam20c deletion, but the migration ability and mineralization ability were significantly weakened. There were tight junctions between Fam20c knockout cells. The expression of mesenchymal cell marker genes and core transcription factors was significantly decreased, and the expression of epithelial cell marker genes was significantly increased. The expression of mesenchymal cell marker proteins was significantly decreased, and the expression of epithelial cell marker proteins was significantly increased. Multiple signalling molecules and pathways involved in MET have changed. Conclusions: Knockdown of Fam20c resulted in MET. Fam20c affects the transcription of key factors in osteoblast MET.

Comparison among methods of effective energy evaluation of corn silage for beef cattle

Wei, Ming,Chen, Zhiqiang,Wei, Shengjuan,Geng, Guangduo,Yan, Peishi Asian Australasian Association of Animal Productio 2018 Animal Bioscience Vol.31 No.6

Objective: This study was conducted to compare different methods on effective energy evaluation of corn silage for beef cattle. Methods: Twenty Wandong bulls (Chinese indigenous yellow cattle) with initial body weight of $281{\pm}15.6kg$, were assigned to 1 of 5 dietary treatments with 4 animals per treatment in a randomized complete block design. Five dietary treatments included group 1 with corn silage only diet, group 2 with corn silage-concentrate basal diet (BD) and 3 groups with 3 test diets, which were the BD partly substituted by corn silage at 10%, 30%, and 60%. The total collection digestion trial was conducted for 5 d for each block after a 10-d adaptation period, and then an open-circuit respiratory cage was used to measure the gas exchange of each animal in a consecutive 4-d period. Results: The direct method-derived metabolizable energy and net energy of corn silage were 8.86 and 5.15 MJ/kg dry matter (DM), expressed as net energy requirement for maintenance and gain were 5.28 and 2.90 MJ/kg DM, respectively; the corresponding regression method-derived estimates were 8.96, 5.34, 5.37, and 2.98 MJ/kg DM, respectively. The direct method-derived estimates were not different (p>0.05) from those obtained using the regression method. Using substitution method, the nutrient apparent digestibility and effective energy values of corn silage varied with the increased corn silage substitution ratio (p<0.05). In addition, the corn silage estimates at the substitution ratio of 30% were similar to those estimated by direct and regression methods. Conclusion: In determining the energy value of corn silage using substitution method, there was a discrepancy between different substitution ratios, and the substitution ratio of 30% was more appropriate than 10% or 60% in the current study. The regression method based on multiple point substitution was more appropriate than single point substitution on energy evaluation of feedstuffs for beef cattle.

A Genetically Engineered Pseudomonas fluorescens Strain Possesses Dual Activity Against Phytopathogenic Fungi and Insects

( Wen Wei Lu ),( Wei Qiong Zhang ),( Yan Bai ),( Ying Ying Fu ),( Jun Chen ),( Xiao Lu Geng ),( Yu Jing Wang ),( Ming Xiao ) 한국미생물 · 생명공학회 2010 Journal of microbiology and biotechnology Vol.20 No.2

An Improved Active Damping Method with Capacitor Current Feedback

Geng, Yi-Wen,Qi, Ya-Wen,Liu, Hai-Wei,Guo, Fei,Zheng, Peng-Fei,Li, Yong-Gang,Dong, Wen-Ming The Korean Institute of Power Electronics 2018 JOURNAL OF POWER ELECTRONICS Vol.18 No.2

Proportional capacitor current feedback active damping (CCFAD) has a limited valid damping region in the discrete time domain as (0, $f_s/6$. However, the resonance frequency ($f_r$) of an LCL-type filter is usually designed to be less than half the sampling frequency ($f_s$) with the symmetry regular sampling method. Therefore, ($f_s/6$, $f_s/2$) becomes an invalid damping region. This paper proposes an improved CCFAD method to extend the valid damping region from (0, $f_s/6$ to (0, $f_s/2$), which covers all of the possible resonance frequencies in the design procedure. The full-valid damping region is obtained and the stability margin of the system is analyzed in the discrete time domain with the Nyquist criterion. Results show that the system can operate stably with the proposed CCFAD method when the resonance frequency is in the region (0, $f_s/2$). The performances at the steady and dynamic state are enhanced by the selected feedback coefficient H and controller gain $K_p$. Finally, the feasibility and effectiveness of the proposed CCFAD method are verified by simulation and experimental results.

Numerical and experimental research on the rock-breaking process of tunnel boring machine normal disc cutters

Geng Qi,Wei Zhengying,Meng Hao,Chen Qiao 대한기계학회 2016 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.30 No.4

A common drawback presented by several numerical rock-breaking studies was that the rocks beneath disc cutters were cut off excessively while the rocks between disc cutters remained, which usually resulted in a smaller cutter spacing than the proper value. To overcome this limitation, the constitutive equations of different rock parts were defined separately using VUMAT, an ABAQUS-based material subroutine. The constitutive model of rock was an application of the Drucker-Prager yield criterion coupled with the Lemaitre damage model. Full-scale rock-breaking tests on a rotary cutting machine were conducted, and 25 groups of orthogonal numerical simulations were carried out. By comparing the normal force, rolling force, and specific energy of numerical results with those of experimental results, the optimal values of the defined parameters D c1 , k, and B were determined to be 9 ☓ 10 -4 , 0.1 and 0.8, respectively. With the presented numerical method and the determined parameters, the influences of cutter spacing on normal force, rolling force, and specific energy were studied. Both the normal and rolling forces of the first cutting generally remained constant, whereas the forces of the second cutting generally increased linearly with the cutter spacing. The optimal cutter spacing for the studied rock type (Hard sand rock collected from West Qinling tunnel) was approximately 72 mm, which was in accordance with the cutter spacing of the tunnel boring machine applied in this tunnel project.

CCDC26 Gene Polymorphism and Glioblastoma Risk in the Han Chinese Population

Wei, Xiao-Bing,Jin, Tian-Bo,Li, Gang,Geng, Ting-Ting,Zhang, Jia-Yi,Chen, Cui-Ping,Gao, Guo-Dong,Chen, Chao,Gong, Yong-Kuan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.8

Background: Glioblastoma (GBM) is an immunosuppressive tumor whose median survival time is only 12-15 months, and patients with GBM have a uniformly poor prognosis. It is known that heredity contributes to formation of glioma, but there are few genetic studies concerning GBM. Materials and Methods: We genotyped six tagging SNPs (tSNP) in Han Chinese GBM and control patients. We used Microsoft Excel and SPSS 16.0 statistical package for statistical analysis and SNP Stats to test for associations between certain tSNPs and risk of GBM in five different models. ORs and 95%CIs were calculated for unconditional logistic-regression analysis with adjustment for age and gender. The SHEsis software platform was applied for analysis of linkage disequilibrium, haplotype construction, and genetic associations at polymorphism loci. Results: We found rs891835 in CCDC26 to be associated with GBM susceptibility at a level of p=0.009. The following genotypes of rs891835 were found to be associated with GBM risk in four different models of gene action: i) genotype GT (OR=2.26; 95%CI, 1.29-3.97; p=0.019) or GG (OR=1.33; 95%CI, 0.23-7.81; p=0.019) in the codominant model; ii) genotypes GT and GG (OR=2.18; 95%CI, 1.26-3.78; p=0.0061) in the dominant model; iii) GT (OR=2.24; 95%CI, 1.28-3.92; p=0.0053) in the overdominant model; iv) the allele G of rs891835 (OR=1.85; 95%CI, 1.14-3.00; p=0.015) in the additive model. In addition, "CG" and "CGGAG" were found by haplotype analysis to be associated with increased GBM risk. In contrast, genotype GG of CCDC26 rs6470745 was associated with decreased GBM risk (OR=0.34; 95%CI, 0.12-1.01; p=0.029) in the recessive model. Conclusions: Our results, combined with those from previous studies, suggest a potential genetic contribution of CCDC26 to GBM progression among Han Chinese.

Comparison of the mechanical performance between two-stage and flat-face cutter head for the rock tunnel boring machine (TBM)

Geng Qi,Wei Zhengying,He Fei,Meng Hao 대한기계학회 2015 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.29 No.5

To overcome the drawbacks of the large flat-face TBM cutter head (for example, enormous rock cutting forces and eccentric forcescause excessive deformation of the cutter head and damage to the main bearing), a two-stage cutter head is introduced. The two-stagecutter head was designed using grey relational analysis method according to the flat-face cutter head applied in the West Qinling Tunnel,China. The mechanical performance analysis models of the two cutter heads in the layered-banded rock (LBR) mixed ground were builtto calculate the cutter heads’ rock cutting thrust and torque, eccentric forces and overturning moments. The two cutter heads’ deformationand stress distribution were also compared. We concluded that with the same penetration, the thrust, torque, eccentric force, overturningmoment, deformation and maximum stress of the two-stage cutter head were smaller than those of the flat-face cutter head.

A New Method for Elimination of Zero-Sequence Voltage in Dual Three-Level Inverter Fed Open-End Winding Induction Motors

Geng, Yi-Wen,Wei, Chen-Xi,Chen, Rui-Cheng,Wang, Liang,Xu, Jia-Bin,Hao, Shuang-Cheng The Korean Institute of Power Electronics 2017 JOURNAL OF POWER ELECTRONICS Vol.17 No.1

Due to the excessive zero-sequence voltage in dual three-level inverter fed open-end winding induction motor systems, zero-sequence circumfluence which is harmful to switching devices and insulation is then formed when operating in a single DC voltage source supplying mode. Traditionally, it is the mean value instead of instantaneous value of the zero-sequence voltage that is eliminated, through adjusting the durations of the operating vectors. A new strategy is proposed for zero-sequence voltage elimination, which utilizes unified voltage modulation and a decoupled SVPWM strategy to achieve two same-sized equivalent vectors for an angle of $120^{\circ}$, generated by two inverters independently. Both simulation and experimental results have verified its efficiency in the instantaneous value elimination of zero-sequence voltage.

Preparation and Characterization of Paclitaxel-Loaded PLGA Nanoparticles Coated with Cationic SM5-1 Single-chain Antibody

( Geng Kou ),( Jie Gao ),( Hao Wang ),( Huai Wen Chen ),( Bo Hua Li ),( Da Peng Zhang ),( Shu Hui Wang ),( Sheng Hou ),( Wei Zhu Qian ),( Jian Xin Dai ),( Yan Qiang Zhong ),( Ya Jun Guo ) 생화학분자생물학회 2007 BMB Reports Vol.40 No.5

Numerical and experimental method to determine the boring diameters of a two-stage TBM cutterhead to prevent rock burst

Geng Qi,Wei Zhengying,Meng Hao 대한기계학회 2014 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.28 No.11

A two-stage tunnel boring machine (TBM) cutterhead with a pilot-enlargement boring method was presented to reduce the risk of rockbursts resulting from boring with a TBM with a large flat-face cutterhead. A reduced scale similarity experiment was designed on rocktunnel boring to determine the boring diameters of the two stages, and numerical simulation models were built. A micron X-ray computerizedtomography system was adopted to examine the failure area of the excavated testing piece and validate the numerical models. Stress distribution and energy release rate (ERR) were used in the numerical simulations to evaluate the rock burst risk of the boringprocess. Experimental and numerical results show that rock burst is prone to occur in the upper, lower, and side areas of the surroundingrock around the tunnel, and risk increases with the lateral pressure coefficient and boring diameter. The optimal boring diameter ratio ofstages 1 to 2 was determined between 0.55 and 0.59. Meanwhile, the ERR of the designed two-stage cutterhead is approximately 60%smaller than that of the flat-face cutterhead.