Based on Digital Elevation Model (DEM) for Farmland Landscape Pattern

Wang Yansong,Wang Huili,LiYanyan,Zhang Pengyan 보안공학연구지원센터 2016 International Journal of Smart Home Vol.10 No.3

Dexamethasone enhances glucose uptake by SGLT1 and GLUT1 and boosts ATP generation through the PPP-TCA cycle in bovine neutrophils

Xinbo Wang,Mingyu Tang,Yuming Zhang,Yansong Li,Jingdong Mao,Qinghua Deng,Shusen Li,Zhenwei Jia,Liyin Du 대한수의학회 2022 Journal of Veterinary Science Vol.23 No.5

Background: Clinical dexamethasone (DEX) treatment or stress in bovines results in extensive physiological changes with prominent hyperglycemia and neutrophils dysfunction. Objectives: To elucidate the effects of DEX treatment in vivo on cellular energy status and the underlying mechanism in circulating neutrophils. Methods: We selected eight-month-old male bovines and injected DEX for 3 consecutive days (1 time/d). The levels of glucose, total protein (TP), total cholesterol (TC), and the proinflammatory cytokines interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF)-α in blood were examined, and we then detected glycogen and adenosine triphosphate (ATP) content, phosphofructosekinase-1 (PFK1) and glucose-6-phosphate dehydrogenase (G6PDH) activity, glucose transporter (GLUT)1, GLUT4, sodium/glucose cotransporter (SGLT)1 and citrate synthase (CS) protein expression and autophagy levels in circulating neutrophils. Results: DEX injection markedly increased blood glucose, TP and TC levels, the Ca2+/P5+ ratio and the neutrophil/lymphocyte ratio and significantly decreased blood IL-1β, IL-6 and TNF-α levels. Particularly in neutrophils, DEX injection inhibited p65-NFκB activation and elevated glycogen and ATP contents and SGLT1, GLUT1 and GR expression while inhibiting PFK1 activity, enhancing G6PDH activity and CS expression and lowering cell autophagy levels. Conclusions: DEX induced neutrophils glucose uptake by enhancing SGLT1 and GLUT1 expression and the transformation of energy metabolism from glycolysis to pentose phosphate pathway (PPP)-tricarboxylic acid (TCA) cycle. This finding gives us a new perspective on deeper understanding of clinical anti-inflammatory effects of DEX on bovine.

Ketohexokinase-dependent metabolism of cerebral endogenous fructose in microglia drives diabetes-associated cognitive dysfunction

Li Yansong,Jiang Tao,Du Mengyu,He Shuxuan,Ning Huang,Cheng Bo,Yan Chaoying,Tang Wenxin,Gao Wei,Guo Hongyan,Li Qiao,Wang Qiang 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Dementia, as an advanced diabetes-associated cognitive dysfunction (DACD), has become the second leading cause of death among diabetes patients. Given that little guidance is currently available to address the DACD process, it is imperative to understand the underlying mechanisms and screen out specific therapeutic targets. The excessive endogenous fructose produced under high glucose conditions can lead to metabolic syndrome and peripheral organ damage. Although generated by the brain, the role of endogenous fructose in the exacerbation of cognitive dysfunction is still unclear. Here, we performed a comprehensive study on leptin receptor-deficient T2DM mice and their littermate m/m mice and revealed that 24-week-old db/db mice had cognitive dysfunction and excessive endogenous fructose metabolism in the hippocampus by multiomics analysis and further experimental validation. We found that the rate-limiting enzyme of fructose metabolism, ketohexokinase, is primarily localized in microglia. It is upregulated in the hippocampus of db/db mice, which enhances mitochondrial damage and reactive oxygen species production by promoting nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4) expression and mitochondrial translocation. Inhibiting fructose metabolism via ketohexokinase depletion reduces microglial activation, leading to the restoration of mitochondrial homeostasis, recovery of structural synaptic plasticity, improvement of CA1 pyramidal neuron electrophysiology and alleviation of cognitive dysfunction. Our findings demonstrated that enhanced endogenous fructose metabolism in microglia plays a dominant role in diabetes-associated cognitive dysfunction and could become a potential target for DACD.

Transfer of Heavy Metals from the Polluted Rhizosphere Soil to Celosia argentea L. in Copper Mine Tailings

Zhangjun Shen,Yupeng Wang,Yansong Chen,Zhen Zhang 한국원예학회 2017 Horticulture, Environment, and Biotechnology Vol.58 No.1

Copper mine tailings are often contaminated by a range of heavy metals, which can restrict plant growth. The aim of this study was to investigate the degree of soil contamination by copper (Cu), zinc (Zn), lead (Pb), cadmium(Cd), chromium (Cr), and manganese (Mn) in a deserted copper mine tailings site, as well as the translocation of theseheavy metals into the rhizosphere soil and the root, fibril and shoot tissues of a tolerant plant, Celosia argentea. Inorder of concentration, the heavy metal concentrations in the soils were Cu ≥ Mn > Zn ≥ Cr > Pb > Cd, and themean concentrations of Cu, Zn, Pb, and Cd in the rhizosphere were 1.8, 1.6, 1.5, and 1.2 times greaterthanin thenon-rhizosphere soil, respectively. The concentrations of all examined heavy metals except for Pb were higher thanthe second-level criterion of the Chinese soil environmental quality standard (CSES) (GB15618-1995) in both soilsamples. The pollution load indexes of Cu, Zn, Pb, Cd, and Cr in the rhizosphere were 28.13, 1.69, 0.148, 8.67, and1.49, respectively, using the CSES. Cu and Cr were mainly stored in the roots of C. argentea, whileCd, Mn, andZn accumulated in the shoots. The translocation factors of Zn, Cd, and Mn were greater than 1, and the Cdenrichment factor of the C. argentea shoots was close to 1. This indicates that C. argentea is a hyperaccumulator ofCd, and could be used in the phytoremediation of Cd-contaminated soils.

Surface Modifications of Titanium Dental Implants with Strontium Eucommia Ulmoides to Enhance Osseointegration and Suppress Inflammation

Avery Rui Sun,Qili Sun,Yansong Wang,Liqiu Hu,Yutong Wu,Fenbo Ma,Jiayi Liu,Xiangchao Pang,Bin Tang 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Background Titanium (Ti) is now widely used as implant material due to its excellent mechanical properties and superior biocompatibilities, while its inert bioactivities might lead to insufficient osseointegration, and limit its performance in dental applications. Methods We introduced a robust and simple approach of modifying titanium surfaces with polysaccharide complexes. Titanium samples were subjected to hydrothermal treatment to create a uniform porous structure on the surface, followed by coating with a bioinspired and self-assembly polydopamine layer. Strontium Eucommia Ulmoides Polysaccharide (EUP-Sr) complexes are then introduced to the polydopamine-coated porous titanium. Multiple morphological and physiochemical characterizations are employed for material evaluation, while cell proliferation and gene expression tests using macrophages, primary alveolar bone osteoblasts, and vascular endothelial cells are used to provide an overall insight into the functions of the product. The significances of statistical differences were analyzed using student’s t-test. Results Microscopic and spectrometric characterizations confirmed that the Ti surface formed a porous structure with an adequate amount of EUP-Sr loading. The attachment was attributed to hydrogen bonding between the ubiquitous glycosidic linkage of the polysaccharide complex and the ring structure of polydopamine, yet the loaded EUP-Sr complex can be gradually released, consequently benefiting the neighboring microenvironment. Cell experiments showed no cytotoxicity of the material, and the product showed promising anti-inflammation, osseointegration, and angiogenesis properties, which were further confirmed by in vivo evaluations. Conclusion We believe the EUP-Sr modified titanium implant is a promising candidate to be used in dental applications with notable osteoimmunomodulation and angiogenesis functions. And the novel technique proposed in this study would benefit the modification of metal/inorganic surfaces with polysaccharides for future research.

H_∞ Control of Networked Control Systems with Packet Disordering with an Improved Prediction-based Method

Yan Song,Jingcheng Wang,Yuanhao Shi,Chuang Li 제어·로봇·시스템학회 2011 International Journal of Control, Automation, and Vol.9 No.5

This paper is concerned with H_∞ control of networked control systems (NCSs) with both network-induced delay and packet disordering taken into account. A switching-based method depends on different cases of prediction numbers is presented to model the NCSs as a switched system. With the application of an improved prediciton-based method to compensate for the time delay and packet disordering, H_∞ controller is designed by solving the linear matrix inequalities (LMIs). The proposed method can be applied variously due to all kinds of prediciton numbers of the consective disordering packet have been considered, which means that our techniques are less conservative than existing lit-eratures. Finally numerical examples and simulations are used to illustrate the effectiveness and valid-ity of the proposed switching-based method and the improved prediction-based H∞ controller design synthesis.