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RISS 인기검색어
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- 저자 : Wang Xuehong (검색결과 4 건)
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Xu Xiong,Xuehong Zhang,Zhenqiang Wu,Zhilong Wang 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.6
Agricultural products exhibit a “buffering nature”during autoclaving and fermentation processes: i.e., thefinal pH of agricultural product medium after Monascusfermentation maintains a certain value. The protein contentof agricultural products strongly affects the bufferingcapacity and an extremely low final pH, below 3, can beachieved by the selection of agricultural products with lowprotein content, such as rice meal and cornmeal. This lowpH can be used to inhibit citrinin biosynthesis duringMonascus fermentation. Thus, optimal selection of agriculturalproducts with low protein content may provide a novelstrategy to inhibit citrinin production in submerged cultureof Monascus anka at a low initial pH for the production ofMonascus pigments. pH values ranging from 2.6 to 3 canbe maintained in Monascus fermentation using cornmeal/wheat starch (30:30 g/L) as substrate at initial pH 2.5,where the concentration of intracellular pigments reaches45 AU at 470 nm while citrinin is undetectable by TLCanalysis.
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Tong Mu,Nengyu Pan,Yingfeng Wang,Xuehong Ren,Tung-Shi Huang 한국섬유공학회 2018 Fibers and polymers Vol.19 No.11
In this study, we synthesized a novel N-halamine precursor, sulfuric acid mono-[2-(4-[4-chloro-6-(2-[4,4- dimethyl-2,5-dioxo-imidazolidin-1-yl]-ethylamino)-[1,3,5]triazin-2-ylamino]-benzenesulfonyl)-ethyl] ester sodium (TB), which contains two reactive groups of monochloro triazine reactive groups and bis-sulphatoethylsolphone reactive groups. The structure of TB is similar to iso-bifunctional group reactive dyes and could be coated on cotton fabrics by covalent bonds through a reactive dyeing process. The cotton coated with TB was characterized by FTIR and SEM. After chlorination, the treated cotton fabrics showed excellent antibacterial efficacy and inactivated all inoculated S. aureus (ATCC 6538) and E. coli O157: H7 (ATCC 43895) within 1 min of contact. Over 85 % of tensile strength retained both in warp and weft directions after treatment and chlorination. Almost 80 % of active chlorine can be regained by treating with household bleach after extensive washing and long time storage.
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Antibacterial and Hydrophilic Modification of PET Fabrics by Electron Beam Irradiation Process
Shumin Zhang,Fang Ding,Yingfeng Wang,Xuehong Ren,Tung-Shi Huang 한국섬유공학회 2020 Fibers and polymers Vol.21 No.5
Electron beam (EB) irradiation has been utilized to modify materials for various applications due to its remarkableadvantages. As an efficient and environmental-friendly way for antibacterial and hydrophilic purposes, EB irradiation wasapplied to modify polyethylene terephthalate (PET) fabrics by grafting with a N-halamine precursor monomer 3-allyl-5,5-dimethylhydantoin (ADMH) and acrylic acid (AA) in this study. The grafted PET fabrics were loaded with silver ions tofurther enhance the antimicrobial efficacy. The hydrophilicity of the modified PET fabrics was evaluated by testing the watercontact angles with different contact times. The breaking strength and thermal stability of the modified swatches werestudied. The UVA light stability results showed the chlorine loading of the modified PET fabrics decreased with the extensionof UVA exposure time, and most chlorine loading could be recovered by re-chlorination. The antibacterial test showed thatthe modified PET swatches can inactivate all inoculated S. aureus and E. coli with short contact times.
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Dongwei Yuan,Li Qing,Lu Xing,Lan Jianfeng,Qiu Zhidong,Wang Xuehong,Wang Junnan,Zheng Xiaojiao,Chen Sifan,Zhang Chong,Jin Junfei 생화학분자생물학회 2024 Experimental and molecular medicine Vol.56 No.-
Acute liver injury is the basis of the pathogenesis of diverse liver diseases. However, the mechanism underlying liver injury is complex and not completely understood. In our study, we revealed that CERK, which phosphorylates ceramide to produce ceramide-1-phosphate (C1P), was the sphingolipid pathway-related protein that had the most significantly upregulated expression during acute liver injury. A functional study confirmed that CERK and C1P attenuate hepatic injury both in vitro and in vivo through antioxidant effects. Mechanistic studies have shown that CERK and C1P positively regulate the protein expression of NRF2, which is a crucial protein that helps maintain redox homeostasis. Furthermore, our results indicated that C1P disrupted the interaction between NRF2 and KEAP1 by competitively binding to KEAP1, which allowed for the nuclear translocation of NRF2. In addition, pull-down assays and molecular docking analyses revealed that C1P binds to the DGR domain of KEAP1, which allows it to maintain its interaction with NRF2. Importantly, these findings were verified in human primary hepatocytes and a mouse model of hepatic ischemia‒reperfusion injury. Taken together, our findings demonstrated that CERK-mediated C1P metabolism attenuates acute liver injury via the binding of C1P to the DGR domain of KEAP1 and subsequently the release and nuclear translocation of NRF2, which activates the transcription of cytoprotective and antioxidant genes. Our study suggested that the upregulation of CERK and C1P expression may serve as a potential antioxidant strategy to alleviate acute liver injury.
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