Clinical Application of Transcatheter Arterial Chemoembolization Combined with Synchronous C-arm Cone-Beam CT Guided Radiofrequency Ablation in treatment of Large Hepatocellular Carcinoma

Wang, Zhi-Jun,Wang, Mao-Qiang,Duan, Feng,Song, Peng,Liu, Feng-Yong,Wang, Yan,Yan, Jie-Yu,Li, Kai,Yuan, Kai Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3

Objective: This work aimed to evaluate the safety and clinical efficacy of transcatheter arterial chemoembolization (TACE) combined with c-arm cone-beam CT guided synchronous radiofrequency ablation (RFA) in treatment of large hepatocellular carcinoma (HCC). Methods: 21 patients with large HCC were studied from January 2010 to March 2012. TACE combined with synchronous C-arm cone-beam CT guided RFA were performed on a total of 25 lesions. Conventional imaging examination (CEUS, enhanced CT or MRI) and AFP detection were regularly conducted to evaluate the technical success rate of combined treatment, complications, treatment response, time without disease recurrence and survival rate. Results: The technical success rate of combined treatment was 100%, without any significant complication. After 1 month, there were 19 cases with complete response and 2 cases with partial response, with an complete response rate of 90.4% (19/21) and a clinical effective rate of 100% (21/21). The complete response rates of single nodular lesions (100%, 17/17) was significantly higher than that of multiple nodular lesions (50%, 2/4) (P<0. 05). During 2 to 28 months of follow-up, in 19 cases with complete response, the average time without disease recurrence was $10.8{\pm}6$ months. The total survival rates of 6, 12 and 18 months in 21 patients were 100%, respectively. Conclusion: TACE combined with synchronous C-arm CT guided RFA is safe and effective for treatment of large HCC. The treatment efficacy for single nodular lesion is better than that for multiple nodular lesions.

Coefficient of Earth Pressure at Rest of a Saturated Artificially Mixed Soil from Oedometer Tests

Jun-Jie Wang,Yang Yang,Jiping Bai,Jian-Yun Hao,Tian-Long Zhao 대한토목학회 2018 KSCE JOURNAL OF CIVIL ENGINEERING Vol.22 No.5

The present study focuses on the coefficient of earth pressure at rest (K0) of a saturated crushed binary soil mixture. The mixture isartificially mixed by crushed sandstone and mudstone particles according to presupposed weight ratios and particle size distributioncurves. The oedometer tests are performed to determine the coefficient K0. From test data, the values of coefficient K0, which rangefrom 0.242 to 0.381, with a mean value of 0.300, are obtained. Just as other mechanical parameters of soils, the K0 value is affectedby many factors. The effects of the properties of test specimen and material are discussed on the basis of the test data. Strong negativecorrelation between the values of K0 and initial dry bulk density of test specimen, one between the values of K0 and median particlesize diameter of test material, and one between the values of K0 and gravel content by weight of test material, respectively, areobserved from the test data. The mudstone particle content by weight of the mixture may also affect the value of K0. The effects ofthese factors on the K0 value may exhibit interlocking effect. Higher interlocking effect results in higher shear strength, and thereforeresults in lower K0 value.

Effects of Soaking and Cyclic Wet-dry Actions on Shear Strength of an Artificially Mixed Sand

Jun-Jie Wang,Yong-Feng Zhou,Xiao Wu,Hui-Ping Zhang 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.4

This study focuses on the effects of soaking action and wetting-drying cycles on the shear strength of a sandstone-mudstone mixture. This mixture is constantly used as a filler in earth structures along bank of harbor or in a large reservoir. Two-type direct shear tests in laboratory, Soaking Direct Shear Test (SDST) and Wetting-Drying Direct Shear Test (WDDST), were carried out. The experimental data indicate that the shear strength is reduced by the soaking action and wetting-drying cycles. While the period of soaking time (t) increased from 24 to 392 hours, the values of initial angle of shearing resistance (φ0) and reducing angle of shearing resistance (φd) reduced from 45.17° to 40.26° and from 19.68° to 12.28°, respectively, along logarithmic curves. With increment of the number of wetting-drying cycles (N) from 1 to 30, the values of φ0 and φd reduced from 40.99° to 38.84° and from 8.76° to 7.58°, respectively, along logarithmic curves. During the soaking action or wetting-drying cycles, the inevitably lessening of the stiffness of soil particle would happen, and hence the shear strength of mixture is decreased. With increment of t or N value, the rates of effects isinstantly reducing.

Retrospective Study of Gemcitabine Based Chemotherapy for Unresectable or Recurrent Esophagus Squamous Cell Carcinoma Refractory to First Line Chemotherapy

Wang, Mei,Gu, Jun,Wang, Hai-Xing,Wu, Mei-Hong,Li, Yong-Mei,Wang, Ya-Jie Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8

Purpose: To investigate the efficacy and toxicity of a combination of gemcitabine with nedaplatin (GN) or cisplatin (GC) for patients with unresectable or recurrent esophagus squamous cell carcinoma. Methods: Gemcitabine was administered at 1 g/m2 intravenously on days 1 and 8; and nedaplatin or cisplatin were administered at 80 mg/m2 intravenously on day 1. We analyzed the response rate, overall survival time, progression-free survival time, and toxicity in 21 patients treated with GN and 27 patients treated with GC. Results: In patients treated with gemcitabine plus nedaplatin, the ORR was 47.6%, the median progression-free survival time was 4.1 months, and the median survival time was 9.3 months. In patients treated with gemcitabine plus cisplatin, the ORR was 48.2%, the median progression-free survival time was 3.9 months, and the median survival time was 9.1 months, respectively. There were no statistically significant differences in ORR, PFS and OS between the two groups. In both, the most commonly observed toxicities were thrombocytopenia and fatigue. Nausea and vomiting was more frequent in the GC group than in the GN group. Conclusion: Gemcitabine based chemotherapy was effective and tolerable for patients with unresectable or recurrent esophagus squamous cell carcinoma refractory to first line chemotherapy.

Mechanistic Analysis of Taxol-induced Multidrug Resistance in an Ovarian Cancer Cell Line

Wang, Ning-Ning,Zhao, Li-Jun,Wu, Li-Nan,He, Ming-Feng,Qu, Jun-Wei,Zhao, Yi-Bing,Zhao, Wan-Zhou,Li, Jie-Shou,Wang, Jin-Hua Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.9

Objectives: To establish a taxol-resistant cell line of human ovarian carcinoma (A2780/Taxol) and investigate its biological features. Methods: The drug-resistant cell line (A2780/Taxol) was established by continuous stepwise selection with increasing concentrations of Taxol. Cell morphology was assessed by microscopy and growth curves were generated with in vitro and in vivo tumor xenograft models. With rhodamine123 (Rh123) assays, cell cycle distribution and the apoptotic rate were analyzed by flow cytometry (FCM). Drug resistance-related and signal associated proteins, including P-gp, MRPs, caveolin-1, PKC-${\alpha}$, Akt, ERK1/2, were detected by Western blotting. Results: A2780/Taxol cells were established with stable resistance to taxol. The drug resistance index (RI) was 430.7. Cross-resistance to other drugs was also shown, but there was no significant change to radioresistance. Compared with parental cells, A2780/Taxol cells were significantly heteromorphous, with a significant delay in population doubling time and reduced uptake of Rh123 (p<0.01). In vivo, tumor take by A2780 cells was 80%, and tumor volume increased gradually. In contrast, with A2780/Taxol cells in xenograft models there was no tumor development. FCM analysis revealed that A2780/Taxol cells had a higher percentage of G0/G1 and lower S phase, but no changes of G2 phase and the apoptosis rate. Expression of P-gp, MRP1, MRP2, BCRP, LRP, caveolin-1, PKC-${\alpha}$, Phospho-ERK1/2 and Phospho-JNK protein was significantly up-regulated, while Akt and p38 MARK protein expression was not changed in A2780/Taxol cells. Conclusion: The A2780/Taxol cell line is an ideal model to investigate the mechanism of muti-drug resistance related to overexpression of drug-resistance associated proteins and activation of the PKC-${\alpha}/ERK$ (JNK) signaling pathway.

Study on Synthesis of PAEKs Containing Triazole Units through Click Reaction and Their Properties

Wang, Lian-Jun,Li, Jie,Wang, Le-Yong Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.9

A series of novel linear aromatic ether ketone polymers containing triazole units were synthesized by click chemistry and their structures and properties were characterized by FT-IR, $^1H$ NMR, GPC, TGA, DSC and WAXD. The results showed that these polymers exhibited relatively small molecular weights distributions, good thermal stability and solvent-repelling which could have potential applications as engineering thermoplastic.

Early Enteral Nutrition and Sepsis-Associated Acute Kidney Injury: A Propensity Score Matched Cohort Study Based on the MIMIC-III Database

Jun Wang,Li Jiang,Sheng Ding,Si-Yi He,Shun-Bi Liu,Zhong-Jie Lu,Yuan-Zhang Liu,Li-Wen Hou,Bin-Su Wang,Jin-Bao Zhang 연세대학교의과대학 2023 Yonsei medical journal Vol.64 No.4

Purpose: We aimed to analyze the optimal timing of enteral nutrition (EN) in the treatment of sepsis and its effect on sepsis-asso ciated acute kidney injury (SA-AKI.)Materials and Methods: The MIMIC-III database was employed to identify patients with sepsis who had received EN. With AKI as the primary outcome variable, receiver operating characteristic (ROC) curves were utilized to calculate the optimal cut-off time of early EN (EEN). Propensity score matching (PSM) was employed to control confounding effects. Logistic regressions and propen sity score-based inverse probability of treatment weighting were utilized to assess the robustness of our findings. Comparisons within the EEN group were performed. Results: 2364 patients were included in our study. With 53 hours after intensive care units (ICU) admission as the cut-off time of EEN according to the ROC curve, 1212 patients were assigned to the EEN group and the other 1152 to the delayed EN group. The risk of SA-AKI was reduced in the EEN group (odds ratio 0.319, 95% confidence interval 0.245–0.413, p<0.001). The EEN patients re ceived fewer volumes (mL) of intravenous fluid (IVF) during their ICU stay (3750 mL vs. 5513.23 mL, p<0.001). The mediating ef fect of IVF was significant (p<0.001 for the average causal mediation effect). No significant differences were found within the EEN group (0–48 hours vs. 48–53 hours), except that patients initiating EN within 48 hours spent fewer days in ICU and hospital. Conclusion: EEN is associated with decreased risk of SA-AKI, and this beneficial effect may be proportionally mediated by IVF volume.

Down-regulated MYH11 Expression Correlates with Poor Prognosis in Stage II and III Colorectal Cancer

Wang, Ren-Jie,Wu, Peng,Cai, Guo-Xiang,Wang, Zhi-Min,Xu, Ye,Peng, Jun-Jie,Sheng, Wei-Qi,Lu, Hong-Fen,Cai, San-Jun Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.17

The MYH11 gene may be related to cell migration and adhesion, intracellular transport, and signal transduction. However, its relationship with prognosis is still uncertain. The aim of this study was to investigate correlations between MYH11 gene expression and prognosis in 58 patients with stage II and III colorectal cancer. Quantitative real-time polymerase chain reaction was performed in fresh CRC tissues to examine mRNA expression, and immunohistochemistry was performed with paraffin-embedded specimens for protein expression. On univariate analysis, MYH11 expression at both mRNA and protein levels, perineural invasion and lymphovascular invasion were related to disease-free survival (p<0.05; log-rank test). Cancers with lower MYH11 expression were more likely to have a poor prognosis. Otherwise, MYH11 expression was unrelated to patient clinicopathological features. On multivariate analysis, low MYH11 expression proved to be an independent adverse prognosticator (p<0.05). These findings show that MYH11 can contribute to predicting prognosis in stage II and III colorectal cancers.

Radixin Knockdown by RNA Interference Suppresses Human Glioblastoma Cell Growth in Vitro and in Vivo

Qin, Jun-Jie,Wang, Jun-Mei,Du, Jiang,Zeng, Chun,Han, Wu,Li, Zhi-Dong,Xie, Jian,Li, Gui-Lin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.22

Radixin, a member of the ERM (ezrin-radixin-moesin) family, plays important roles in cell motility, invasion and tumor progression. It is expressed in a variety of normal and neoplastic cells, including many types of epithelial and lymphoid examples. However, its function in glioblastomas remains elusive. Thus, in this study, radixin gene expression was first examined in the glioblastoma cells, then suppressed with a lentivirus-mediated short-hairpin RNA (shRNA) method.We found that there were high levels of radixin expression in glioblastoma U251cells. Radixin shRNA caused down-regulation of radixin gene expression and when radixin-silenced cells were implanted into nude mice, tumor growth was significantly inhibited as compared to blank control cells or nonsense shRNA cells. In addition, microvessel density in the tumors was significantly reduced. Thrombospondin-1 (TSP-1) and E-cadherin were up-regulated in radixin- suppressed glioblastoma U251 cells. In contrast, MMP9 was down-regulated. Taken together, our findings suggest that radixin is involved in GBM cell migration and invasion, and implicate TSP-1, E-cadherin and MMP9 as metastasis-inducing factors.

Study on Synthesis of PAEKs Containing Triazole Units through Click Reaction and Their Properties

Lian-jun Wang,Jie Li,Le-yong Wang 대한화학회 2011 Bulletin of the Korean Chemical Society Vol.32 No.9

A series of novel linear aromatic ether ketone polymers containing triazole units were synthesized by click chemistry and their structures and properties were characterized by FT-IR, ^1H NMR, GPC, TGA, DSC and WAXD. The results showed that these polymers exhibited relatively small molecular weights distributions,good thermal stability and solvent-repelling which could have potential applications as engineering thermoplastic.