C2 Superior Facetal Osteotomy: A Novel Technique in Complex Craniovertebral Junction Surgery for C1 Lateral Mass Screw Placement

Deepak Kumar Singh,Shankar Diwakar,Vipin Kumar Chand,Singh Rakesh Kumar,Singh Neha 대한척추외과학회 2023 Asian Spine Journal Vol.17 No.6

Complex craniovertebral junction (CVJ) defects account for a considerable proportion of CVJ diseases. Given the heavily assimilated C1, an unfavorable C1–C2 joint orientation, an overriding C2 superior facet, a low-hanging occiput, and an abnormal vertebral artery course with a high-riding vertebral artery, placement of C1 lateral mass screws might be difficult. To address this, a novel technique for placing C1 lateral mass screws that avoid vertebral artery injury, low-hanging occiput, and overriding C2 superior facet was developed in this study. This approach enables firm fixation of C1–C2 even in difficult situations where the placement of the C1 lateral mass is challenging.

Ruptured mirror DACA aneurysm: A rare case report and review of literature

Deepak Kumar Singh,Prevesh Kumar Sharma,Arun Kumar Singh,Vipin Kumar Chand 대한뇌혈관외과학회 2023 Journal of Cerebrovascular and Endovascular Neuros Vol.25 No.3

Bilateral distal anterior cerebral artery (DACA) aneurysms also called “kissing aneurysms” or “mirror aneurysm” are extremely rare, accounting for only 0.2% of all intracranial aneurysms. There have only been a few examples of mirror DACA aneurysms reported in the literature. Here, we report a rare case of mirror DACA aneurysm in a middle aged female with its successful clipping. Patient was admitted with severe headache and altered sensorium. Computed tomography (CT) head was suggestive of anterior inter-hemispheric hematoma. Digital subtraction angiography (DSA) was done which was suggestive of two distal anterior cerebral artery aneurysms located at same anatomical position. It was treated through microsurgical clipping. Mirror image DACA aneurysms are rare occurrence. All patients with ruptured DACA aneurysms should have angiography with 3D reconstruction studies. This aids in determining the aneurysm’s morphology and planning treatment accordingly.

Protein Engineering Approaches in the Post-Genomic Era

Singh, Raushan Kumar,Lee, Jung-Kul,Selvaraj, Chandrabose,Singh, Ranjitha,Li, Jinglin,Kim, Sang-Yong,Kalia, Vipin C. Bentham Science Publishers 2018 Current protein & peptide science Vol.19 No.1

<P> Proteins are one of the most multifaceted macromolecules in living systems. Proteins have evolved to function under physiological conditions and, therefore, are not usually tolerant of harsh experimental and environmental conditions. The growing use of proteins in industrial processes as a greener alternative to chemical catalysts often demands constant innovation to improve their performance. Protein engineering aims to design new proteins or modify the sequence of a protein to create proteins with new or desirable functions. With the emergence of structural and functional genomics, protein engineering has been invigorated in the post-genomic era. The three-dimensional structures of proteins with known functions facilitate protein engineering approaches to design variants with desired properties. There are three major approaches of protein engineering research, namely, directed evolution, rational design, and de novo design. Rational design is an effective method of protein engineering when the threedimensional structure and mechanism of the protein is well known. In contrast, directed evolution does not require extensive information and a three-dimensional structure of the protein of interest. Instead, it involves random mutagenesis and selection to screen enzymes with desired properties. De novo design uses computational protein design algorithms to tailor synthetic proteins by using the three-dimensional structures of natural proteins and their folding rules. The present review highlights and summarizes recent protein engineering approaches, and their challenges and limitations in the post-genomic era. </P>

Morin ameliorates myocardial injury in diabetic rats via modulation of inflammatory pathways

Vipin Kumar Verma,Salma Malik,Ekta Mutneja,Anil Kumar Sahu,Vaishali Prajapati,Prashant Mishra,Jagriti Bhatia,Dharamveer Singh Arya 한국실험동물학회 2024 Laboratory Animal Research Vol.40 No.1

Background: High blood glucose levels in diabetes lead to vascular inflammation which accelerates atherosclerosis. Herein, Morin was orally administered in male Wistar rats, at the dose of 40 mg/kg for 28 days, and on the 27th and 28th day, ISO was administered to designate groups at the dose of 85 mg/kg s.c., to induce myocardial infarction. Results: Free radical generation, including ROS, in diabetes following ISO administration, leads to the activation of both intrinsic and extrinsic pathways of apoptosis. Morin significantly (p ≤ 0.05) reduced oxidative stress (GSH, MDA, SOD), cardiac injury markers (CK-MB, LDH), inflammation (TNF, IL-6), and apoptosis (Bax, BCl2, Caspase-3). In addition, it also reduced insulin and blood glucose levels. Akt/eNOS, Nrf2/HO-1, MAPK signaling pathways, and Insulin signal transduction pathways were positively modulated by morin pre-treatment. Conclusions: Morin attenuated oxidative stress and inflammation and also modified the activity of various molecular pathways to mitigate cardiomyocyte damage during ISO-induced MI in diabetic rats.

Green and proficient process for industrial-scale preparation of Gymnema sylvestre standardized-extract enriched with Gymnemic acids through polymer-matrix-adsorption to reduce hyperglycemia

Deepak Kumar,Vipin Kumar Singh,Narayan Prasad Yadav,Sudeep Tandon,Karuna Shanker,Chandan Singh Chanotiya,Narendra Kumar,Puja Khare,Anirban Pal,Debabrata Chanda,Dharmendra Saikia,Yusuf Hussain,Abha Mee 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.116 No.-

Gymnema sylvestre is an important medicinal plant, but a method for preparing standardized extractenriched with biomarkers is not available. The practiced crude hydro-alcoholic extracts of G. sylvestrecontained a few percentage of gymnemic acids (GAs). The enrichment of GAs in preprared extracts isessential from a pharmacology point of view. Presently, a scale-up process has been developed forpreparing G. sylvestre standardized extract (Gym-EEXT). Crude extract (Gym-Crude) was prepared in10-kg batches to obtain a yield of 268.1 mg/g, but GAs was merely 9 mg/g (GA-IV 6.7 mg/g, and GAXVII2.3 mg/g) in 30 % ethanol–water solvent at 50 C in 5 h. Further, Gym-Crude was processed throughpolymer-matrix-adsorption techniques for getting Gym-EEXT (150.9 mg/g) with improved percentage ofGAs (>10 %) comprised of deacylgymnemic acid (DGA: 8.9 mg/g), gymnemagenin (2.3 mg/g), GA-IV(78.6 mg/g), and GA-XVII (10.2 mg/g). For safety aspects, the pesticide residues, solvent residues, heavymetal, aflatoxins, and mutagenicity in Gym-EEXT were below the detection limits. Hence, Gym-EEXT wasenriched with GAs, and fully safe with compliance according to USP-561. An insignificant effect of Gym-EEXT on vitals of experimental mice was observed in acute and sub-acute oral-toxicityexperiments. Gym-EEXT significantly improved the blood glucose metabolism, and hence, it potentiallyreduces hyperglycaemia. Unique chromatographic protocols were developed for the isolation of biomarkerson a 1-kg scale from Gym-Crude with yield of gymnemagenin (5.5 %), and DGA (4.6 %). Finally, a novel,scientifically validated, and the scale-up process has been developed for the preparation of Gym-EEXTalong with an exclusive protocol for isolating its biomarkers.

SiO₂ microparticles with carbon nanotube-derived mesopores as an efficient support for enzyme immobilization

Kumar, Ashok,Park, Gi Dae,Patel, Sanjay K.S.,Kondaveeti, Sanath,Otari, Sachin,Anwar, Muhammad Zahid,Kalia, Vipin C.,Singh, Yogendra,Kim, Sun Chang,Cho, Byung-Kwan,Sohn, Jung-Hoon,Kim, Dong Rip,Kang, Y Elsevier 2019 CHEMICAL ENGINEERING JOURNAL -LAUSANNE- Vol.359 No.-

<P><B>Abstract</B></P> <P>Novel mesoporous SiO<SUB>2</SUB> microparticles were synthesized by spray pyrolysis using multiwalled carbon nanotubes (MCNTs) as a template. The synthesized multicompartment structure with uniform pores of 12.0 nm was used to immobilize lipase from <I>Thermomyces lanuginosus</I>. The total surface area of mesoporous SiO<SUB>2</SUB> microparticles prepared from silica colloidal solution was increased by 26-folds compared to that of dense SiO<SUB>2</SUB> particles (494 vs 19.0 m<SUP>2</SUP> g<SUP>−1</SUP>, respectively). Mesoporous SiO<SUB>2</SUB> particles showed 236% higher protein loading for lipase, than dense SiO<SUB>2</SUB> particles. The maximum velocity (<I>V</I> <SUB>max</SUB>) and catalytic efficiencies of immobilized lipase were 3.80 and 5.90 folds higher than that of free enzyme. Contact angle analysis revealed increased hydrophobicity of the mesoporous particles, which is advantageous for lid opening at the active center, and increased activity after immobilization. We next developed a lipase/SiO<SUB>2</SUB>/glassy carbon electrode (GCE) biosensors. Cyclic voltammetric results showed linear responses of the lipase/SiO<SUB>2</SUB>/GCE bioelectrode towards tributyrin (50–300 mg dL<SUP>−1</SUP>) as a surface-limited reaction in Tris-HCl buffer. After 12 repetitive uses, dense SiO<SUB>2</SUB>- and mesoporous SiO<SUB>2</SUB>-bound lipase retained 74.2 and 95.4% of its original activities, respectively. Thus, given their desirable characteristics and industrial utility, greatly porous SiO<SUB>2</SUB> particles may provide an excellent support for enzyme immobilization in biosensor development or biocatalysis in organic media.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Mesoporous SiO<SUB>2</SUB> is synthesized by spray pyrolysis using carbon nanotubes as a template. </LI> <LI> Mesoporous SiO<SUB>2</SUB> microparticles have been successfully used to immobilize enzyme. </LI> <LI> Mesoporous SiO<SUB>2</SUB> provides an excellent support for enzyme in biosensor or biocatalysis. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Ecobiotechnological Approach for Exploiting the Abilities of Bacillus to Produce Co-polymer of Polyhydroxyalkanoate.

Kumar, Prasun,Singh, Mamtesh,Mehariya, Sanjeet,Patel, Sanjay K S,Lee, Jung-Kul,Kalia, Vipin C Association of Microbiologists of India 2014 Indian journal of microbiology Vol.54 No.2

<P>Ecobiotechnological approach is an attractive and economical strategy to enrich beneficial microbes on waste biomass for production of Polyhydroxyalkanoate (PHA). Here, six strains of Bacillus spp. were used to produce co-polymers of PHA from pea-shells. Of the 57 mixed bacterial cultures (BCs) screened, two of the BCs, designated as 5BC1 and 5BC2, each containing 5 strains could produce PHA co-polymer at the rate of 505-560?mg/l from feed consisting of pea-shell slurry (PSS, 2?% total solids) and 1?% glucose (w/v). Co-polymer production was enhanced from 65-560?mg/l on untreated PSS to 1,610-1,645?mg/l from PSS treated with defined hydrolytic bacteria and 1?% glucose. Supplementation of the PSS hydrolysate with sodium propionate enabled 5BC1 to produce co-polymer P(3HB-co-3HV) with a 3HV content up to 13?% and a concomitant 1.46-fold enhancement in PHA yield. Using the principles of ecobiotechnology, this is the first demonstration of PHA co-polymer production by defined co-cultures of Bacillus from biowaste as feed under non-axenic conditions.</P>

Biological methanol production by immobilized <i>Methylocella tundrae</i> using simulated biohythane as a feed

Patel, Sanjay K.S.,Singh, Raushan K,Kumar, Ashok,Jeong, Jae-Hoon,Jeong, Seong Hun,Kalia, Vipin C.,Kim, In-Won,Lee, Jung-Kul Elsevier 2017 Bioresource technology Vol.241 No.-

<P><B>Abstract</B></P> <P>Biohythane may be used as an alternative feed for methanol production instead of costly pure methane. In this study, methanol production potential of <I>Methylocella tundrae</I> immobilized through covalent immobilization, adsorption, and encapsulation was evaluated. Cells covalently immobilized on groundnut shells and chitosan showed a relative methanol production potential of 83.9 and 91.6%, respectively, compared to that of free cells. The maximum methanol production by free cells and cells covalently immobilized on groundnut shells and chitosan was 6.73, 6.20, and 7.23mM, respectively, using simulated biohythane as a feed. Under repeated batch conditions of eight cycles, cells covalently immobilized on chitosan and groundnut shells, and cells encapsulated in sodium-alginate resulted in significantly higher cumulative methanol production of 37.76, 31.80, and 25.58mM, respectively, than free cells (18.57mM). This is the first report on immobilization of methanotrophs on groundnut shells and its application in methanol production using biohythane as a feed.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Biohythane is used as a feed to produce methanol by <I>Methylocella tundrae</I>. </LI> <LI> Compared to pure CH<SUB>4</SUB> as a feed, biohythane results in 1.9-fold higher methanol production. </LI> <LI> Covalently immobilized cells result in higher methanol production than free cells. </LI> <LI> Repeated use of the immobilized cells founds effective to improve methanol production. </LI> </UL> </P>

Efficacy and tolerability of exclusive enteral nutrition in adult patients with complicated Crohn’s disease

( Sanchit Sharma ),( Arti Gupta ),( Saurabh Kedia ),( Samagra Agarwal ),( Namrata Singh ),( Sandeep Goyal ),( Saransh Jain ),( Vipin Gupta ),( Pabitra Sahu ),( Sudheer Kumar Vuyyuru ),( Bhaskar Kante 대한장연구학회 2021 Intestinal Research Vol.19 No.3

Background/Aims: Exclusive enteral nutrition (EEN), an established modality for pediatric Crohn’s disease (CD) is seldomly utilized in adults. The present study reports the outcome of EEN in adult CD patients at a tertiary care hospital in India. Methods: This was a retrospective analysis of CD patients who received EEN as a sole modality/adjunct to other treatment. The primary and secondary outcomes changed in Crohn’s Disease Activity Index (CDAI), and clinical response (decline in CDAI >70), respectively, at 4 and 8 weeks. Subgroup analysis evaluated response across different phenotypes, EEN formulations and prior treatment. Linear mixed effect model was created to assess the predictors of EEN response. Results: Thirty-one CD patients received EEN over median duration of 4 weeks (range, 2-6 weeks). CDAI showed a significant improvement post EEN at 4 (baseline 290 [260-320] vs. 240 [180-280], P=0.001) and 8 weeks (baseline 290 [260-320] vs. 186 [160-240], P=0.001), respectively. The cumulative clinical response rates at 4 and 8 weeks were 37.3% and 80.4% respectively. The clinical response rates at 8 weeks across B1 (n=4), B2 (n=18), and B3 (n=9) phenotypes were 50%, 78.8%, and 100% respectively (log-rank test, P=0.093). The response rates at 8 weeks with polymeric (n=8) and semi-elemental diet (n=23) were 75% and 82.6% respectively (log-rank test, P=0.49). Baseline CDAI (odds ratio, 1.008; 95% confidence interval, 1.002-1.017; P=0.046) predicted response to EEN. Conclusions: EEN was effective in inducing clinical response across different phenotypes of CD. Baseline disease activity remained the most important predictor of clinical response to EEN. (Intest Res 2021;19:291-300)