The Inhibition of Fibrosis and Inflammation in Obstructive Kidney Injury via the miR-122-5p/SOX2 Axis Using USC-Exos

Wenjun Lu,Yujun Guo,Hengchen Liu,Tingting Zhang,Mingzhao Zhang,Xiangqi Li,Zhou Li,Manyu Shi,Zhitao Jiang,Zheng Zhao,Shulong Yang,Zhaozhu Li 한국생체재료학회 2024 생체재료학회지 Vol.28 No.00

Background: Fibrosis and inflammation due to ureteropelvic junction obstruction substantially contributes to poor renal function. Urine-derived stem-cell-derived exosomes (USC-Exos) have therapeutic effects through paracrine. Methods: In vitro, the effects of USC-Exos on the biological functions of HK-2 and human umbilical vein endothelial cells were tested. Cell inflammation and fibrosis were induced by transforming growth factor-β1 and interleukin-1β, and their anti-inflammatory and antifibrotic effects were observed after exogenous addition of USC-Exos. Through high-throughput sequencing of microRNA in USC-Exos, the pathways and key microRNAs were selected. Then, the antifibrotic and anti-inflammatory effects of exosomal miR-122-5p and target genes were verified. The role of the miR-122-5p/SOX2 axis in anti-inflammatory and antifibrotic effects was verified. In vivo, a rabbit model of partial unilateral ureteral obstruction (PUUO) was established. Magnetic resonance imaging recorded the volume of the renal pelvis after modeling, and renal tissue was pathologically analyzed. Results: We examined the role of USC-Exos and their miR-122-5p content in obstructive kidney injury. These Exos exhibit antifibrotic and anti-inflammatory activities. SOX2 is the hub gene in PUUO and negatively related to renal function. We confirmed the binding relationship between miR-122-5p and SOX2. The anti-inflammatory and antifibrotic effects of miR-122-5p were inhibited, indicating that miR-122-5p has anti-inflammatory and antifibrotic effects by inhibiting SOX2 expression. In vivo, the PUUO group showed typical obstructive kidney injury after modeling. After USC-Exo treatment, the shape of the renal pelvis shown a remarkable improvement, and inflammation and fibrosis decreased. Conclusions: We confirmed that miR-122-5p from USC-Exos targeting SOX2 is a new molecular target for postoperative recovery treatment of obstructive kidney injury.

Epidemiologic characteristics and a prognostic nomogram for patients with vulvar cancer: results from the Surveillance, Epidemiology, and End Results (SEER) program in the United States, 1975 to 2016

Shiyuan Wei,Lu Li,Tingting Yi,Licong Su,Qi Gao,Liangzhi Wu,Zhenbo OuYang 대한부인종양학회 2023 Journal of Gynecologic Oncology Vol.34 No.6

Objective: To elucidate clinical characteristics and build a prognostic nomogram for patients with vulvar cancer. Methods: The study population was drawn from the Surveillance, Epidemiology, and End Results (SEER) database. Patients were randomly assigned to training and validation sets. Cox proportional hazards model and competing risk model were used to identify the prognostic parameters of overall survival (OS) and cancer-specific survival (CSS) to construct a nomogram. The nomogram was assessed by concordance index (C-index), area under the curve (AUC), calibration plot, and decision curve analysis (DCA). Results: A total of 20,716 patients were included in epidemiological analysis, of whom 7,025 patients were selected in survival analysis, including 4,215 and 2,810 in training and validation sets, respectively. The multivariate Cox model showed that the predictors for OS were age, marital status, histopathology, differentiation and tumor node metastasis (TNM) stages, whether to undergo surgery and chemotherapy. However, the predictors for CSS were age, race, differentiation and TNM stages, whether to undergo surgery and radiation. The C-index for OS and CSS in the training set were 0.76 and 0.80. The AUC in the training set for 1-, 3- and 5-year OS and CSS were 0.84, 0.81, 0.80 and 0.88, 0.85, 0.83, respectively, which was similar in the validation set. The calibration curves showed good agreement between prediction and actual observations. DCA revealed that the nomogram had a better discrimination than TNM stages. Conclusions: The nomogram showed accurate prognostic prediction in OS and CSS for vulvar cancer, which could provide guidance to clinical practice.

A New Statistical Time of Arrival Ranging Algorithm

Xiaolin Liang,Tingting Lu,Hao Zhang,T. Aaron. Gulliver 보안공학연구지원센터 2015 International Journal of Hybrid Information Techno Vol.8 No.8

Major ginsenosides from Panax ginseng promote aerobic cellular respiration and SIRT1-mediated mitochondrial biosynthesis in cardiomyocytes and neurons

Huang, Qingxia,Lou, Tingting,Lu, Jing,Wang, Manying,Chen, Xuenan,Xue, Linyuan,Tang, Xiaolei,Qi, Wenxiu,Zhang, Zepeng,Su, Hang,Jin, Wenqi,Jing, Chenxu,Zhao, Daqing,Sun, Liwei,Li, Xiangyan The Korean Society of Ginseng 2022 Journal of Ginseng Research Vol.46 No.6

Background: Aerobic cellular respiration provides chemical energy, adenosine triphosphate (ATP), to maintain multiple cellular functions. Sirtuin 1 (SIRT1) can deacetylate peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α) to promote mitochondrial biosynthesis. Targeting energy metabolism is a potential strategy for the prevention and treatment of various diseases, such as cardiac and neurological disorders. Ginsenosides, one of the major bioactive constituents of Panax ginseng, have been extensively used due to their diverse beneficial effects on healthy subjects and patients with different diseases. However, the underlying molecular mechanisms of total ginsenosides (GS) on energy metabolism remain unclear. Methods: In this study, oxygen consumption rate, ATP production, mitochondrial biosynthesis, glucose metabolism, and SIRT1-PGC-1α pathways in untreated and GS-treated different cells, fly, and mouse models were investigated. Results: GS pretreatment enhanced mitochondrial respiration capacity and ATP production in aerobic respiration-dominated cardiomyocytes and neurons, and promoted tricarboxylic acid metabolism in cardiomyocytes. Moreover, GS clearly enhanced NAD⁺-dependent SIRT1 activation to increase mitochondrial biosynthesis in cardiomyocytes and neurons, which was completely abrogated by nicotinamide. Importantly, ginsenoside monomers, such as Rg1, Re, Rf, Rb1, Rc, Rh1, Rb2, and Rb3, were found to activate SIRT1 and promote energy metabolism. Conclusion: This study may provide new insights into the extensive application of ginseng for cardiac and neurological protection in healthy subjects and patients.

A Novel Non-Line of Sight Identification Algorithm in the60GHz Wireless Communication Systems

Xiaolin Liang,Hao Zhang,Tingting Lu,Xuerong Cui 보안공학연구지원센터 2016 International Journal of Smart Home Vol.10 No.4

Autophagy inhibition contributes to epigallocatechin-3-gallate-mediated apoptosis in papillary thyroid cancer cells

Bu Ling,Zheng Tingting,Mao Chaoming,Fei Wu,Mou Xiao,Xu Chengcheng,Luo Xuan,Lu Qingyan,Dong Liyang,Wang Xuefeng 대한독성 유전단백체 학회 2021 Molecular & cellular toxicology Vol.17 No.4

Background Epigallocatechin-3-gallate is a natural polyphenolic compound that induces apoptosis in papillary thyroid cancer cells. However, its underlying molecular mechanism was not completely clarified. Objectives The present study demonstrated the role of apoptosis and autophagy in EGCG-treated papillary thyroid cancer cells and the relationship between these processes. Results EGCG significantly suppressed the viability of TPC-1 papillary thyroid cancer cells at an IC50 of 17.2 μM. EGCG induced TPC-1 cell apoptosis and cell cycle arrest at S phase and downregulated the protein expression of cyclin A and cyclin-dependent kinase-2. EGCG decreased reactive oxygen species levels, upregulated Bax expression, downregulated Bcl-2 expression and increased cytochrome C levels in the cytosol. Treatment with EGCG also increased the levels of cleaved caspase 3, cleaved caspase 9 and cleaved poly(ADP-ribose) polymerase. EGCG induced an autophagic response via the upregulation of the autophagy-related protein LC3-II and suppression of the AKT/mTOR signalling pathway. Autophagy inhibition further enhanced EGCG-induced cell apoptosis and ROS suppression, which indicated that autophagy played a cytoprotective role in EGCG-treated TPC-1 cells. Conclusion Taken together, these results demonstrated that autophagy inhibition was beneficial to EGCG–mediated apoptosis in papillary thyroid cancer cells. Background Epigallocatechin-3-gallate is a natural polyphenolic compound that induces apoptosis in papillary thyroid cancer cells. However, its underlying molecular mechanism was not completely clarified. Objectives The present study demonstrated the role of apoptosis and autophagy in EGCG-treated papillary thyroid cancer cells and the relationship between these processes. Results EGCG significantly suppressed the viability of TPC-1 papillary thyroid cancer cells at an IC50 of 17.2 μM. EGCG induced TPC-1 cell apoptosis and cell cycle arrest at S phase and downregulated the protein expression of cyclin A and cyclin-dependent kinase-2. EGCG decreased reactive oxygen species levels, upregulated Bax expression, downregulated Bcl-2 expression and increased cytochrome C levels in the cytosol. Treatment with EGCG also increased the levels of cleaved caspase 3, cleaved caspase 9 and cleaved poly(ADP-ribose) polymerase. EGCG induced an autophagic response via the upregulation of the autophagy-related protein LC3-II and suppression of the AKT/mTOR signalling pathway. Autophagy inhibition further enhanced EGCG-induced cell apoptosis and ROS suppression, which indicated that autophagy played a cytoprotective role in EGCG-treated TPC-1 cells. Conclusion Taken together, these results demonstrated that autophagy inhibition was beneficial to EGCG–mediated apoptosis in papillary thyroid cancer cells.

Synthesis and Magnetic Properties of FeCo Alloy Nanowires via AC Frequency Conversion Electrodeposition

Lirong Wang,Shunzhen Feng,Tingting Lu,Lizhen Zhen,Jiwei Zhang,Lihu Liu 한국자기학회 2022 Journal of Magnetics Vol.27 No.3

Binary alloy nanowires of Fe3Co7 system are synthesized in the highly ordered porous anodic aluminum oxide (PAA) templates by AC frequency (5, 50, 120, and 200 Hz) conversion electrodeposition method. High resolution field emission transmission electron microscope (HRTEM) and Lorenz TEM (LTEM) were used to study the morphology and the magnetic domain structure of the Fe3Co7 alloy nanowires, respectively. Magnetic measurements showed that the M-H curves presented a discontinuity in a restricted magnetic field, but with high squareness (about 90 %). Quantitative local measurements of the magnetic properties of the nanowires are explored by differential phase contrast (DPC) imaging. The magnetic properties, however, were significantly affected by the AC frequency, which is correlated with the specific magnetic structure formation of Bloch line. We reported herein a detailed investigation on Fe3Co7 alloy nanowires and their magnetic properties.

Outage Probability Analysis of the VAF Relaying M2M Networks

Lingwei Xu,Hao Zhang,Tingting Lu,T. Aaron Gulliver 보안공학연구지원센터 2015 International Journal of Hybrid Information Techno Vol.8 No.5

Based on variable-gain amplify-and-forward (VAF) relaying scheme, the lower bound on outage probability (OP) of the mobile-relay-based mobile-to-mobile (M2M) networks over N-Nakagami fading channels is investigated in this paper. By the method of the harmonic mean of positive random variables, the exact closed form expression of the lower bound on OP is derived. Then the OP performance under different conditions is evaluated through numerical simulations. The numerical simulation results coincide with the theoretical results well, and the accuracy of the analytical results is verified. The simulation results showed that: the fading coefficient, the number of cascaded components, the relative geometrical gain, and the power-allocation parameter have an important influence on the OP performance.

Performance Analysis of the Home M2M Cooperative Networks

Lingwei Xu,Hao Zhang,Tingting Lu,T A Gulliver 보안공학연구지원센터 2015 International Journal of Smart Home Vol.9 No.4

Based on decode-and-forward (DF) relaying scheme, the exact closed-form expression of the outage probability (OP) for the home mobile-to-mobile (M2M) cooperative networks over N-Nakagami fading channels is derived in this paper. Then the OP performance under different conditions is evaluated through numerical simulations. The numerical simulation results coincide with the theoretical results well, and the accuracy of the theoretical results is verified. The simulation results showed that: the fading coefficient, the number of cascaded components, the relative geometrical gain, and the power-allocation parameter have an important influence on the OP performance.

Performance Analysis of the MRC M2M System

Lingwei Xu,Hao Zhang,Tingting Lu,Xing Liu,T. Aaron Gulliver 보안공학연구지원센터 2015 International Journal of Future Generation Communi Vol.8 No.2

The average symbol error probability (ASEP) of a mobile-to-mobile (M2M) system employing maximal ratio combining (MRC) over N-Nakagami fading channels is investigated in this paper. The characteristic function (CF) of the signal-to-noise ratio (SNR) at the MRC output is used to derive exact ASEP expressions for several q-ary modulation schemes, including phase shift keying (PSK) modulation, quadrature amplitude modulation (QAM), and pulse amplitude modulation (PAM). Then the ASEP performance under different channel conditions is evaluated through numerical simulation to verify the analysis. These results show that the performance of the MRC M2M system is improved as the number of diversity branches or the fading coefficient is increased. Conversely, performance is degraded as the number of cascaded components is increased.