Optimum synthesis of esomeprazole catalyzed by Rhodococcus rhodochrous ATCC 4276 through response surface methodology

Yuanyuan Zhang,Qiuxiang Zhao,Hui Tang,Huiling Li,Depeng Li,Zhiyong Wang,Xin Gao,Fanye Wang 한국화학공학회 2021 Korean Journal of Chemical Engineering Vol.38 No.5

Enantiopure esomeprazole is an important drug in the treatment of gastric ulcer. The asymmetric sulfoxidation of omeprazole thioether was catalyzed by immobilized cells of a mutant of Rhodococcus rhodocrous ATCC 4276 to synthesize esomeprazole. The bioreaction was carried out in a biphasic system (chloroform-water), at a high substrate concentration (200mM), and optimized using response surface methodology (RSM). The optimal yield of esomeprazole obtained was 94.8% with e.e. (>99%) without the formation of the sulfone form as a byproduct, under the optimal conditions: the concentration of immobilized cells, 283.5 g/L, the incubation temperature, 37.05 oC, and pH of phosphate buffer, 7.35, respectively. A quadratic polynomial model was developed with R2 of 0.9998, which indicates that the model predicts the observed data with very high accuracy. The mutant exhibited a high enantioselective activity and substrate and product tolerance. The small size of immobilized cell beads (0.5-1 mm) creates a large reaction interface. The aerated flask provides enough oxygen for a high concentration of cells. The significant improvement of substrate tolerance may mainly be attributed to employing the chloroform-water biphasic system because organic substrates may be partitioned in the organic phase, eliminating potential damage and inhibition to cells. Based on the above, the asymmetric sulfoxidation catalyzed by immobilized bacterial cells is therefore more promising for efficient synthesis of chiral sulfoxides.

Reliability assessment for multi-source data of mechanical parts of civil aircraft based on the model

Yuanyuan Guo,Youchao Sun,Longbiao Li,Xiaoming Tang 대한기계학회 2019 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.33 No.7

In view of the abnormal, missing and multi-source reliability data, a complete reliability data processing method for civil machinery parts are formed. The abnormal data in the original reliability data set is screened out by Pauta criterion. The vacant data is supplemented by linear interpolation. Each subjective index weight of multi-source data is determined by analytic hierarchy process (AHP) and Euclidean distance. Each objective index weight is determined by the discrete degree of the index weight. The comprehensive reliability model about a certain mechanical part with multi-source data is established and validated to be practical through case analysis. The proposed method obtains more accurate assessment of part reliability, and is thus valuable for the safe operation and maintenance of civil aircraft.

Novel Mutations in the UNC13D Gene Carried by a Chinese Neonate with Hemophagocytic Lymphohistiocytosis

Yuanyuan Chen,Zhujun Wang,Yuping Cheng,Yongmin Tang 연세대학교의과대학 2013 Yonsei medical journal Vol.54 No.4

Hemophagocytic lymphohistiocytosis (HLH) in different ethnicities has been described in the literature, but few cases in patients of Chinese descent have been reported. Here, we describe the case of a Chinese neonate presenting with HLH carrying novel, compound heterozygous mutations of the UNC13D gene, including [c.2295_2298delGCAG, p.Glu765Aspfs*27] in exon 23, c.-250C>T, c.1+30G>A, c.279C>T, c.888G>C, c.18+36A>G, c.20-48T>C, c.1977C>T, c.2296C>T, c.24-46C>T, c.26-9_26-8insC, c.2599A>G, c.28+48C>T and c.3198A>G, some of which have not been reported in the literature. Cytokine profile analyses were performed in this patient, and the results were consistent with our previous findings in HLH patients. Cytokine profile monitoring may be helpful in differentiating among various clinical phases of HLH.

Knowledge management pattern differences in oriental and occidental firms

Shukun Tang,Yuanyuan Fang,Xiaojiong Wang 인하대학교 정석물류통상연구원 2009 인하대학교 정석물류통상연구원 학술대회 Vol.2009 No.10

This is a cross-disciplinary study exploring the roots of knowledge management pattern differences in Oriental and Occidental firms from the perspective of cultural studies, Beginning with a critical review of the literature on knowledge management (KM) studies from the view of culture, this paper employs Edward T. Hall’s elaboration of high-context and low-context cultures to account for the nation-specific features in various processes of MK operation. specifically focusing on knowledge sharing and innovation. Two case of knowledge management patterns, in firms from th United States and Japan, are contrasted. Analysis results shed light on the underlying causes for KM pattern differences in firms with different cultural background. Plus, Strengths and weaknesses of each KM pattern resulting from different cultures are identified and implications on a better convergence of cultural background and KM practice are proposed.

Antioxidant and Anti-anemia Activity of Heme Iron Obtained from Bovine Hemoglobin

Ning Tang,Yuanyuan Zhu,Hong Zhuang 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.2

The industrial by-product bovine blood was used to prepare heme iron. Obtained heme iron exhibited strong in vitro antioxidant activities. Heme iron exhibited a significantly (p<0.05) higher DPPH radical scavenging activity than ascorbic acid at low concentrations. The effects of heme iron in iron deficient anemic rats were evaluated. Heme iron exhibited an effective restorative action by returning hemoglobin, red blood cell, hematocrit, mean corpuscular hemoglobin concentrations, and serum iron levels in iron-deficient animals to normal or better values. Rats in the medium dosage group (2 mg of Fe/kg of body weight) experienced better iron bioavailability than rats in other treatment groups. The activities of antioxidant enzymes were enhanced while malondialdehyde levels were reduced in iron deficient anemic rats. Heme iron obtained from bovine blood can be used as a safe and efficient new iron supplement.

Contribution of Urine and Dung Patches from Grazing Sheep to Methane and Carbon Dioxide Fluxes in an Inner Mongolian Desert Grassland

Jiang, Yuanyuan,Tang, Shiming,Wang, Chengjie,Zhou, Pei,Tenuta, Mario,Han, Guodong,Huang, Ding Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.2

The effects of sheep urine and dung patches on methane ($CH_4$) and carbon dioxide ($CO_2$) fluxes were investigated during the summer-autumn in 2010, to evaluate their contribution to climate change in a desert grassland in Inner Mongolia, China. Results indicate that the cumulative $CH_4$ emissions for dung patches, urine patches and control plots were -0.076, -0.084, and -0.114 g/$m^2$ and these were net $CH_4$ sinks during the measured period. The level of $CH_4$ intake from urine and dung plots decreased 25.7%, and 33.3%, respectively, compared with a control plot. $CO_2$ fluxes differed (p<0.01) in urine plots, with an average of 569.20 mg/$m^2$/h compared with control plots (357.62 mg/$m^2$/h) across all sampling days. Dung patches have cumulative $CO_2$ emissions that were 15.9% higher compared with the control during the 55-d period. Overall, sheep excrement weakened $CH_4$ intake and increased $CO_2$ emissions.

Transcriptome sequencing and comparative analysis of differentially expressed genes in the roots of Musa Paradisiaca under salt stress

Ji Fusang,Tang Lu,Yang Zhen,Li Yuanyuan,Wang Wenchang,Xu Ya,Li Shuangmei,Li Xinguo 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.3

Salt stress severely impacts plant growth and development. Banana, a salt-sensitive plant, has been studied for decades with respect to its physiological and biochemical performances under salt stress. To understand the salinity tolerance mechanisms of banana, the RNA-seq technique is applsied to analyze the transcriptomic changes on roots treated with salt (60 mM NaCl) for 12 h and 24 h in comparison with those cultured in normal condition. Totally, 1466 and 2089 diferentially expressed genes (DEGs), including 542 up- and 924 down-regulated-; 507 up- and 1582 down-regulated genes are identifed after salt treatment at 12 h and 24 h, respectively. Functional annotation analysis indicates that a large number of genes can be involved in several aspects of salt stress response including stress signal transduction, ROS scavenging, osmoregulation and transcription factors (TFs). This study is the frst report of a comprehensive transcriptome-based gene expression profle to analyze banana response to salt stress. The outcomes of this study could facilitate further dissecting the molecular mechanism underlying salt stress response and provide a valuable platform for further genetic improvement of salt tolerance in banana breeding programs.

The Role of Wheat Germ Agglutinin in the Attachment of Pseudomonas sp. WS32 to Wheat Root

Jian Zhang,Liyuan Meng,Yuanyuan Cao,Huiping Chang,Zhongyou Ma,Leni Sun,Ming Zhang,Xinyun Tang 한국미생물학회 2014 The journal of microbiology Vol.52 No.12

Wheat germ agglutinin (WGA), which is secreted on thesurface of wheat root, has been defined as a protein that reversiblyand non-enzymatically binds to specific carbohydrates. However, little attention has been paid to the functionof WGA in the attachment of bacteria to their host plants. The aim of this study was to investigate the role of WGA inthe attachment of Pseudomonas sp. WS32 to wheat roots. Wheat roots were initially treated with double-distilled water,WGA-H (WGA solution that was heated at 100°C for 15 min)and WGA, independently. Subsequently, the roots were coincubatedwith cell solutions (109 cells/ml). A dilution platemethod using a solid nutrient medium was employed to determinethe adsorption of WS32 to wheat roots. WGA waslabeled with fluorescein isothiocyanate and detected usingthe fluorescent in situ hybridization (FISH) technique. Thenumber of adsorptive WS32 cells on wheat roots was significantlyincreased when the wheat roots were pretreatedwith WGA, compared with the control treatment (p = 0.01). However, WGA-H failed to increase the amount of bacterialcells that attached to the wheat roots because of the lossof its physiological activity. The FISH assay also revealedthat more cells adhered to WGA-treated wheat roots than tocontrol or WGA-H-treated roots. The results indicated thatWGA can mediate Pseudomonas strain WS32’s adherenceto wheat seedling roots. The findings of this study provide abetter understanding of the processes involved in plant-microbe interactions.

Effects of three drying methods on polyphenol composition and antioxidant activities of Litchi chinensis Sonn.

Si Tan,Jianmin Tang,Wenjing Shi,Zhuwei Wang,Yuanyuan Xiang,Tingwei Deng,Xiaoxu Gao,Wenfeng Li,Shengyou Shi 한국식품과학회 2020 Food Science and Biotechnology Vol.29 No.3

The aim of this study was to investigate the effects of three different drying methods, freeze drying (FD), vacuum drying (VD) and oven drying (OD) on phenolic contents and antioxidant activities of litchi fruits. 20 polyphenols were exactly identified in the litchi fruits by UPLC-QqQ/MS. Significant losses were observed in the contents of total polyphenols and antioxidant activities in the dried litchi when compared with the fresh litchi. Principle component analysis indicated that there was significant difference of phenolic component between the use of thermal drying (VD and OD) and FD. Our results suggest that FD is the optimum drying method for litchi fruits considering the content of total polyphenols and antioxidant activities.

LINC00174 Facilitates Proliferation and Migration of Colorectal Cancer Cells via MiR-3127-5p/ E2F7 Axis

( Yuhong Ma ),( Yuzhen Li ),( Yuanyuan Tang ),( Ning Tang ),( Dengke Wang ),( Xiaofei Li ) 한국미생물 · 생명공학회 2021 Journal of microbiology and biotechnology Vol.31 No.8

The literature indicates that LINC00174 promotes the growth of colorectal cancer (CRC) cells, but its research needs to be enriched. We tried to explore the function and mechanism of LINC00174 in CRC cell proliferation and migration. Bioinformatics analysis predicted the binding relationship and expressions of lncRNA, miRNA and mRNA. Clinical study analyzes the relationship between LINC00174 and clinical data characteristics of CRC patients. The expressions of LINC00174, miR-3127-5p and E2F7 were verified by RT-qPCR, and the combination of the two was verified by dual luciferase analysis and RNA immunoprecipitation as needed. Western blot was used to detect the expression of EMT-related protein and E2F7 protein. Functional experiments were used to evaluate the function of the target gene on CRC cells. LINC00174 was up-regulated in CRC clinical samples and cells and was related to the clinical characteristics of CRC patients. High-expression of LINC00174, contrary to the effect of siLINC00174, promoted cell viability, proliferation, migration and invasion, up-regulated the expressions of N-Cadherin, Vimentin, E2F7, and inhibited the expression of E-Cadherin. MiR-3127-5p was one of the targeted miRNAs of LINC00174 and was down-regulated in CRC samples. In addition, miR-3127-5p mimic partially reversed the malignant phenotype of CRC cells induced by LINC00174. Besides, E2F7 was a target gene of miR-3127-5p, and LINC00174 repressed miR-3127-5p to regulate E2F7. Our research reveals that LINC00174 affected the biological characteristics of CRC cells through regulated miR-3127-5p/ E2F7 axis.