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        Antimicrobial Activity of Phenolic-Rich Extracts from Mango Seed Kernel on Microorganisms

        Tam Dang Thi Thu,Binh Ly Nguyen,Nhan Tran Chi,Loc Nguyen Bao,Phuong Nguyen Nhat Minh 한국미생물·생명공학회 2023 한국미생물·생명공학회지 Vol.51 No.4

        Replacing synthetic additives to preserve food products with natural antimicrobial compounds needs to be considered due to public health. In the present study, the phenolic extract from mango seed kernel (MSK) with the total phenolic content (TPC) value of 5300 ± 380 mg gallic acid equivalent (GAE)/L was used for evaluating the antimicrobial properties against five types of bacterial strains, including Listeria monocytogenes, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Salmonella typhimurium in vitro. This extract was also used for in situ method on the storage of Pangasianodon hypophthalmus fish fillets. The minimum inhibitory concentration (MIC) values for all tested strains were determined at an average concentration of 1325 mg GAE/L. Furthermore, the minimum bactericidal concentration (MBC) values of E. coli, S. aureus, and S. typhimurium were seen at 5300 mg GAE/L, while the extract did not show eliminations in the growth of the remaining strains. The bacterial inhibition speed of the extract illustrated that the concentration equal MIC value eliminated S. typhimurium growth after 24 h, the 4 times MIC value had remarkable effects on S. aureus growth after the 9 h of incubation, and 24 h of incubation for E. coli, L. monocytogenes, P. aeruginosa. Additionally, the MSK extract could inhibit the growth of P. aeruginosa on fish fillets in 4 days of storage. These results provide important evidence for the utilization of MSK as a natural source of antimicrobial agents in food products.

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        High-performance nonenzymatic electrochemical glucose biosensor based on AgNP-decorated MoS2 microflowers

        Dinh Van Tuan,Dang Thi Thuy Ngan,Dao Vu Phuong Thao,Nguyen Thi Nguyet,Nguyen Thi Thuy,Nguyen Phuong Thuy,Vu Van Thu,Vuong-Pham Hung,Phuong Dinh Tam 한국물리학회 2022 Current Applied Physics Vol.43 No.-

        A facile hydrothermal route was used to synthesize silver nanoparticle (AgNP)-decorated microflower molybdenum disulfide (MoS2-MF) for bio-electrochemical platform fabrication to detect nonenzymatic glucose concentration. The morphologies of the materials were studied by scanning electron microscopy, and their structural characteristics were analyzed by X-ray diffractometry and energy-dispersive X-ray spectroscopy. The electrochemical characteristics of the AgNPs/MoS2-MF/PtE biosensor were studied by cyclic voltammetry. The obtained data indicated that the developed nonenzymatic glucose sensor has a large linear response between 1.0 and 15.0 mM, a limit of detection of as low as 1.0 mM, and a sensitivity of 46.5 μA nM-1 cm-2. The biosensor also displayed outstanding selectivity, stability, reproducibility, and repeatability. Additionally, the AgNPs/MoS2-MF/ PtE biosensor was utilized to detect glucose concentration in real sample and showed practical application potential for glucose detection.

      • KCI등재

        Effect of nanostructured MoS2 morphology on the glucose sensing of electrochemical biosensors

        Dinh Van Tuan,Dang Thi Thuy Ngan,Nguyen Thi Thuy,Hoang Lan,Nguyen Thi Nguyet,Vu Van Thu,Vuong-Pham Hung,Phuong Dinh Tam 한국물리학회 2020 Current Applied Physics Vol.20 No.9

        In this study, the effects of the morphological characteristics of MoS2 nanomaterials on the glucose sensing of electrochemical biosensors were explored. Nanostructured MoS2 materials, including nanoparticles (NPs), nanoflowers (NFs), and nanoplatelets (NPLs), were prepared via a simple hydrothermal method. The structure and morphological characteristics of MoS2 nanomaterials were examined through X-ray diffraction, field emission scanning electron microscopy, and Raman spectroscopy. Electrochemical properties were analyzed through cyclic voltammetry. Results showed that the obtained sensitivity was 64, 68.7, and 77.6 μAmM 1 cm 2 for MoS2 NP-, MoS2 NF-, and MoS2 NPL-based biosensors, respectively. The limit of detection (LOD) of all MoS2-based glucose biosensors was 0.081 mM. In addition, the pH, temperature, glucose oxidase (GOx) concentration, reproducibility, specificity, and stability of glucose biosensors with different MoS2 morphologies were also investigated and indicated the oxidation current response of the MoS2 NPL-based glucose biosensor was higher than that of MoS2 NF- and NP-based biosensors.

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