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Lee, Song-Yi,Lee, Hakbong,Lee, Hye-Kyeong,Lee, Seung-Won,Ha, Sung Chul,Kwon, Taejoon,Seo, Jeong Kon,Lee, Changwook,Rhee, Hyun-Woo American Chemical Society 2016 ACS central science Vol.2 No.8
<▼1><P/><P>Mammalian target of rapamycin (mTOR) signaling is a core pathway in cellular metabolism, and control of the mTOR pathway by rapamycin shows potential for the treatment of metabolic diseases. In this study, we employed a new proximity biotin-labeling method using promiscuous biotin ligase (pBirA) to identify unknown elements in the rapamycin-induced interactome on the FK506-rapamycin binding (FRB) domain in living cells. FKBP25 showed the strongest biotin labeling by FRB–pBirA in the presence of rapamycin. Immunoprecipitation and immunofluorescence experiments confirmed that endogenous FKBP25 has a rapamycin-induced physical interaction with the FRB domain. Furthermore, the crystal structure of the ternary complex of FRB–rapamycin–FKBP25 was determined at 1.67-Å resolution. In this crystal structure we found that the conformational changes of FRB generate a hole where there is a methionine-rich space, and covalent metalloid coordination was observed at C2085 of FRB located at the bottom of the hole. Our results imply that FKBP25 might have a unique physiological role related to metallomics in mTOR signaling.</P></▼1><▼2><P>A proximity biotin-labeling method revealed that FKBP25 is a strong rapamycin-induced interaction partner of FK506-rapamycin binding (FRB). This interaction was supported by the crystal structure of the ternary complex of FKBP25−rapamycin−FRB.</P></▼2>
Simple and rapid detection of bacteria using a nuclease-responsive DNA probe
Lee, Kyung Jin,Lee, Wang Sik,Hwang, Ahreum,Moon, Jeong,Kang, Taejoon,Park, Kyoungsook,Jeong, Jinyoung The Royal Society of Chemistry 2018 The Analyst Vol.143 No.1
<P>We demonstrate simple and rapid bacterial detection using a nuclease-responsive DNA probe. The probe consisting of a fluorescent dye and a quencher at the 5′ and 3′ termini, respectively, was designed to be cleaved by nucleases such as endonucleases, exonucleases, and DNases, which are released from bacteria using an optimized lysis buffer. The fluorescence signal of the cleaved DNA probe correlates with the number of Gram-negative <I>Escherichia coli</I> and Gram-positive <I>Staphylococcus aureus</I>, and the detection limit was 10<SUP>3</SUP> CFU for <I>E. coli</I> and 10<SUP>4</SUP> CFU for <I>S. aureus</I>. Moreover, this method is specific for live bacteria and takes just one minute to get the signal including sample collection. These features make the present bacterial detection method a powerful on-site bacterial contamination assay which is simple, rapid, and quantitative.</P>
Precision Targeting Tumor Cells Using Cancer-specific InDel Mutations with CRISPR-Cas9
Taejoon Kwon,Jae Sun Ra,Soyoung Lee,In-Joon Baek,Keon Woo Khim,Eun A Lee,Eun Kyung Song,Daniyar Otarbayev,Woojae Jung,Yong Hwan Park,Minwoo Wie,Juyoung Bae,Himchan Cheng,Jun Hong Park,Namwoo Kim,Yuri 한국고분자학회 2024 한국고분자학회 학술대회 연구논문 초록집 Vol.49 No.1
Lee, Taejoon,Park, Keun-Young,Pyo, Dongjin The Korean Society of Analytical Science 2017 분석과학 Vol.30 No.1
An analytical method for determining potential endocrine disruptors (bisphenol A, 2-chlorophenol, 2,4-dichlorophenol, 2,4,6-trichlorophenol, pentachlorophenol, p-t-butylphenol, p-pentylphenol, p-hexylphenol, p-t-octylphenol, p-heptylphenol, nonylphenol) by solid-phase extraction (SPE) and High Perfomance Liquid Chromatography(HPLC) equipped with fluorescence and variable wavelength detector has been developed. The SPE process for sample concentration was performed on a commercially available Oasis HLB cartridge packed with polymeric sorbents. The effect of elution solvent and elution volume on the recoveries of the analytes were investigated with HPLC. Average recovery of >85% was achieved with 60mg sorbents using 5mL of methanol as elution solvent. Phenolic compounds in canned drinks, beverages and water samples were surveyed by this proposed method.
Lee, Wang Sik,Cho, Hyun-Ju,Kim, Eungwang,Huh, Yang Hoon,Kim, Hyo-Jeong,Kim, Bongsoo,Kang, Taejoon,Lee, Jeong-Soo,Jeong, Jinyoung The Royal Society of Chemistry 2019 Nanoscale Vol.11 No.7
<P>As nano- and micro-sized plastics accumulate in the environment and the food chain of animals, including humans, it is imperative to assess the effects of nanoplastics in living organisms in a systematic manner, especially because of their ability to adsorb potential toxicants such as pollutants, heavy metals, and organic macromolecules that coexist in the environment. Using the zebrafish embryo as an animal model, we investigated the bioaccumulation and <I>in vivo</I> toxicity of polystyrene (PS) nanoplastics individually or in combination with the Au ion. We showed that smaller PS nanoplastics readily penetrated the chorion and developing embryos and accumulated throughout the whole body, mostly in lipid-rich regions such as in yolk lipids. We also showed that PS nanoplastics induced only marginal effects on the survival, hatching rate, developmental abnormalities, and cell death of zebrafish embryos but that these effects were synergistically exacerbated by the Au ion in a dose- and size-dependent manner. Such exacerbation of toxicity was well correlated with the production of reactive oxygen species and the pro-inflammatory responses synergized by the presence of PS, supporting the combined toxicity of PS and Au ions. The synergistic effect of PS on toxicity appeared to relate to mitochondrial damage as determined by ultrastructural analysis. Taken together, the effects of PS nanoplastics were marginal but could be a trigger for exacerbating the toxicity induced by other toxicants such as metal ions.</P>
이상우(Lee Sangwoo),권오성(Kwon Osung),이상철(Lee Sangcheol),배준형(Bae Junhyung),박태준(Park Taejoon),강진규(Kang Jinkyu),이동하(Lee Dongha) 한국태양에너지학회 2012 한국태양에너지학회 학술대회논문집 Vol.2012 No.3
In recent years, understanding the dynamics of DC distribution system has become critically important due mainly to the increasing needs for the interconnection of DC distributed generators and the (DC-based) electric vehicle (EV) charging systems. In this paper, the characteristics of the DC grid system connected to the compact proton exchange membrane fuel cell (PEMFC) has been studied. In particular, the voltage and current transient phenomena were measured by varying the load of the DC grid system. Also, the voltage and current ripple were measured at the different load conditions. Our experimental results clearly manifested that the study contributes to the establishment of fundamental method to characterize the small DC grid system including distributed generation.