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Plant Regeneration and Bulblet Formation of Allium wakegi Araki
Song,Won-seob,Yang,Deok-Chun,Yoon,Jae-Ho,Ryu,Sang-Hyun 한국자원식물학회 2004 Plant Resources Vol.7 No.1
Allium wakegi was cultured shoot tip in the condition of light culture. The Allium wakegi added plant growth regulator was observed of plant regeneration and bulblet formation. Callus Induction and growing rate was the best of 78% when added alone 2,4-D 0.5mg/L. In the formation of shoot, its regeneration rate was 96% when added BA 0.5mg/L in the light culture condition. When BA 0.5mg/L and NAA 0.5mg/L mixed and BA 0.5 mg/L and NAA 1.0mg/L mixed, the rates were 99% and 97% respectively, and these conditions were suitable for forming shoot. In the formation of roots, when added NAA 2.0mg/L in the light culture condition, the regeneration rate was 90.6 % and the roots were abnormal. When added NAA 1.0mg/L, the rate was 82 % and the highest. In the formation of bulbs, when BA 05mg/L and NAA 1.0mg/L mixed, the root generantion and its size in the bulbs was the best compare to other treatment experiments.
Identification of the Gene Products Responsible for F Plasmid Partitioning
KIM, SUNG0UK,NAGAI, KAZUO,TAMURA, GAKUZO,YU, JU-HYUN,BOK, SONG-HAE 한국미생물 · 생명공학회 1993 Journal of microbiology and biotechnology Vol.3 No.4
DNA subfragments, sopA, sopB and sopC which help to maintain the stability of an on C plasmid, were derived from a mini-F plasmid DNA (EcoRI restriction fragment f5) after digestion with restriction endonuclease, and cloned in the vector plasmid pBR322. The recombinant plasmids obtained were introduced into E. coli KY7231 and E. coli CSR603 strains, and proteins specified by the mini-F fragments were analysed by SDS-PAGE. Two proteins encoded by the F fragments were detected, and their molecular weights were 41,000 and 37,000 daltons. Fluorography after one and two dimensional gel electrophoresis of the lysates showed that these two proteins had been overproduced in the cells which were allowed to incorporate radioactive amino acid after plasmid amplification by chloramphenicol treatment. The isoelectric points of sopA and sopB proteins were 6.6 and 7.0, respectively.
Song, Hyun Seok,Jin, Hye Jun,Ahn, Sae Ryun,Kim, Daesan,Lee, Sang Hun,Kim, Un-Kyung,Simons, Christopher T.,Hong, Seunghun,Park, Tai Hyun American Chemical Society 2014 ACS NANO Vol.8 No.10
<P>The sense of taste helps humans to obtain information and form a picture of the world by recognizing chemicals in their environments. Over the past decade, large advances have been made in understanding the mechanisms of taste detection and mimicking its capability using artificial sensor devices. However, the detection capability of previous artificial taste sensors has been far inferior to that of animal tongues, in terms of its sensitivity and selectivity. Herein, we developed a bioelectronic tongue using heterodimeric human sweet taste receptors for the detection and discrimination of sweeteners with human-like performance, where single-walled carbon nanotube field-effect transistors were functionalized with nanovesicles containing human sweet taste receptors and used to detect the binding of sweeteners to the taste receptors. The receptors are heterodimeric G-protein-coupled receptors (GPCRs) composed of human taste receptor type 1 member 2 (hTAS1R2) and human taste receptor type 1 member 3 (hTAS1R3), which have multiple binding sites and allow a human tongue-like broad selectivity for the detection of sweeteners. This nanovesicle-based bioelectronic tongue can be a powerful tool for the detection of sweeteners as an alternative to labor-intensive and time-consuming cell-based assays and the sensory evaluation panels used in the food and beverage industry. Furthermore, this study also allows the artificial sensor to exam the functional activity of dimeric GPCRs.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancac3/2014/ancac3.2014.8.issue-10/nn502926x/production/images/medium/nn-2014-02926x_0005.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nn502926x'>ACS Electronic Supporting Info</A></P>
Durability Evaluation of Thermal Barrier Coating (TBC) at High Temperature
Song, Hyun Woo,Kim, Yong Seok,Koo, Jae Mean,Seok, Chang Sung Trans Tech Publications, Ltd. 2013 Applied Mechanics and Materials Vol. No.
<P>Thermal barrier coating (TBC) which protects the gas turbine from high temperature is damaged by repeated thermal fatigue [1,2]. Generally, damage of top coating of thermal barrier coating is resulted in damage to the entire gas turbine. Thus, the durability of the thermal barrier coating should be evaluated to protect the gas turbine from damage. In general, the major cause of delamination in the top coating is the thermal stress at its interface according to the change in temperature [. In this research, parallel stress at the top coating interface (S11) was verified as the major cause of delamination by finite element analysis. In order to evaluate the durability of the TBC, we need information about the parallel strength at the interface, but it is difficult to measure. Furthermore, we verified the relationship between the stress perpendicular to the interface (S22) and the stress parallel to the interface (S11) by finite element analysis. The durability of the thermal barrier coating was evaluated by comparison of the results of the bond strength test of Kim <I>et al</I>. with the results of the finite element analysis of this research.</P>
Sang Hyun Joo,Jin Kyun Park,Eunyoung Emily Lee,Yeong Wook Song,Sung-Soo Yoon 대한혈액학회 2016 Blood Research Vol.51 No.3
BackgroundSince cell turnover in the hematopoietic system constitutes a major source of uric acid (UA) production, we investigated whether hematopoietic stem cell transplantation (HSCT) is associated with significant changes in serum UA levels in patients with hemato-logical disorders. MethodsPatients who underwent HSCT at our institution between 2001 and 2012 were retro-spectively enrolled. Serum UA levels at 3 months before, 1 week before, and 3 months and 1 year after HSCT were examined. ResultsComplete clinical and laboratory information including data regarding UA levels was avail-able for 93 patients. At baseline, the mean UA level was 4.9±2.1 mg/dL, with an overall prevalence of hyperuricemia of 15% (defined as serum UA>6.8 mg/dL). Mean UA levels tended to be higher in patients with acute myeloid leukemia (4.8±2.0 mg/dL) and non-Hodgkin lymphoma (5.1±2.3 mg/dL) and lower in patients with aplastic anemia (mean, 4.2±1.8 mg/dL). UA levels dropped during myeloablative conditioning, reaching a nadir on the day of HSCT (3.27±1.4 mg/dL). Over the 3 months following HSCT, UA levels rose sharply (5.0±2.1 mg/dL) and remained stable up to 1 year after HSCT (5.5±1.6mg/dL). UA levels in HSCT recipients at 12 months correlated with those of their respective graft donors (Pearson r=0.406, P=0.001).ConclusionHSCT is associated with significant changes in uric acid levels in patients with hematologic disorders.
Song, Bong-Seok,Yoon, Seung-Bin,Sim, Bo-Woong,Kim, Young-Hyun,Cha, Jae-Jin,Choi, Seon-A,Jeong, Kang-Jin,Kim, Ji-Su,Huh, Jae-Won,Lee, Sang-Rae,Kim, Sang-Hyun,Kim, Sun-Uk,Chang, Kyu-Tae Commonwealth Scientific and Industrial Research Or 2014 Reproduction, fertility, and development Vol.26 No.3
<P>Despite the positive roles of histone deacetylase inhibitors in somatic cell nuclear transfer (SCNT), few studies have evaluated valproic acid (VPA) and its associated developmental events. Thus, the present study was conducted to elucidate the effect of VPA on the early development of bovine SCNT embryos and the underlying mechanisms of action. The histone acetylation level of SCNT embryos was successfully restored by VPA, with optimal results obtained by treatment with 3mM VPA for 24h. Importantly, the increases in blastocyst formation rate and inner cell mass and trophectoderm cell numbers were not different between the VPA and trichostatin A treatment groups, whereas cell survival was notably improved by VPA, indicating the improvement of developmental competence of SCNT embryos by VPA. Interestingly, VPA markedly reduced the transcript levels of endoplasmic reticulum (ER) stress markers, including sXBP-1 and CHOP. In contrast, the levels of GRP78/BiP, an ER stress-alleviating transcript, were significantly increased by VPA. Furthermore, VPA greatly reduced cell apoptosis in SCNT blastocysts, which was further evidenced by the increased levels of the anti-apoptotic transcript Bcl-xL and decreased level of the pro-apoptotic transcript Bax. Collectively, these results suggest that VPA enhances the developmental competence of bovine SCNT embryos by alleviating ER stress and its associated developmental damage.</P>
Hyun Wook Kim,Min Sung Choi,Ko Eun Hwang,Dong Heon Song,Yong Jae Kim,Youn Kyung Ham,Seong Jin Chang,Yun Bin Lim,Yun Sang Choi 한국축산식품학회 2014 한국축산식품학회지 Vol.34 No.2
This study is conducted to evaluate the effects of the mixing ratio between pork loin and chicken breast for textural and sensory properties of emulsion sausages. Meat homogenates are prepared by using five mixing ratios between pork loin and chicken breast (100:0, 70:30, 50:50, 30:70, and 0:100), and the emulsion sausages are also formulated with five mixing ratios. The additions of chicken breast increase the salt soluble protein solubility due to high pH levels of chicken breast, thereby resulting in the reduction of cooking losses. In addition, the apparent viscosity of meat homogenates increase with increasing amounts of chicken breast. In terms of emulsion sausages formulated with pork loin and chicken breast, the addition of chicken breast above 50% may contribute to a softer and more flexible texture of emulsion sausages. For sensory evaluations, an increase in the added amount of chicken breast contributes to a rich umami taste and deeper flavor within the emulsion sausages, resulting in the high overall acceptance score for the formulation of 0-30% pork loin and 70-100% chicken breast. Therefore, the optimal mixing ratios between pork loin and chicken breast are 0-30% and 70-100% for enhancing the textural and sensory properties of emulsion sausages
Song, Donghoon,Kang, Moon-Sung,Lee, Yong-Gun,Cho, Woohyung,Lee, Jung Hyun,Son, Taewook,Lee, Kyoung Jun,Nagarajan, S.,Sudhagar, P.,Yum, Jun-Ho,Kang, Yong Soo The Royal Society of Chemistry 2012 Physical chemistry chemical physics Vol.14 No.2
<p>A new I<SUP>−</SUP>/(SeCN)<SUB>2</SUB> redox mediator has favorable properties for dye-sensitized solar cells (DSCs) such as less visible light absorption, higher ionic conductivity, and downward shift of redox potential than I<SUP>−</SUP>/I<SUB>3</SUB><SUP>−</SUP>. It was then applied for DSCs towards increasing energy conversion efficiency, giving a new potential for improving performance.</p> <P>Graphic Abstract</P><P>A novel I<SUP>−</SUP>/(SeCN)<SUB>2</SUB> redox mediator, for the first time, showed favourable electrochemical properties for DSCs, resulting in an increased energy conversion efficiency. <img src='http://pubs.rsc.org/ej/CP/2011/c1cp23457a/c1cp23457a-ga.gif'> </P>