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Sumi Lee,Byungsik Shin,Minhyo Kang,Jiyoung Hong,Dasol Bae,Yaeji Yoon,Byungmin Song 한국응용곤충학회 2018 한국응용곤충학회 학술대회논문집 Vol.2018 No.04
지의류 추출물이 흰줄숲모기(Aedes albopictus)에 대하여 살충활성 및 생장 억제에 미치는 영향을 조사하였다. 2종의 지의류의 메탄올 추출물이 흰줄숲모기 3령 유충에 미치는 치사농도, 사충수, 생장억제를 알아보기 위한 실험을 진행한 결과Parmelia sp.엽상체 추출물경우 LC50농도는 0.13%로 나타났으며 Porpidia albocaerulescens의 LC50농 도는 0.45%로 나타나 Parmelia sp.의 추출물이 더 높은 살충활성을 보였다. 24h, 48h, 72h의 노출시간 따른 농도별 사충 수를 조사한 결과, 전반적으로 지의류의 메탄올 추출물이 고농도일수록, 노출시간이 길어질수록 유충의 사충 수는 증가하였다. Parmelia sp. 엽상체의 추출물에서 사육한 유충의 경우 용화시간은 대조군보다 지연되었고, 추출물의 농도가 높을수록 더 많은 시간이 지연된 것으로 보아 지의류 추출물이 흰줄숲모기 유충의 생장을 억제하는 효과가 있음을 확인하였다.
Yunjung Lee,SuMi Lee,Honggyun Kim,SangYoon Lee,Eunji Kwak,JunHwan Bae,Hayoung Jeong,HyeRin Shin,Youngjae Cho,Mi-Jung Choi 한국산업식품공학회 2018 학술대회 및 심포지엄 Vol.2018 No.04
Probiotics are defined as advantageous microorganisms to human when they are ingested. However, without any protection, the viability of microbes and their adhesive ability to surface of colon decreases through acidic condition such as stomach and intestines. Therefore, many studies have been conducted to figure out to enhance not only the viability of probiotics, but also its adhesion for increasing effect of probiotics. In this study, extrusion method was conducted to encapsulate Enterococcus faecium. E. faecium-alginate solution was injected to CaCl2 solution with regular side air injection. To prevent coagulation of beads, stirring was conducted in CaCl2 solution and encapsulated alginate-Ca2+ microspheres were produced. For optimal encapsulation condition, air pressure was 100 mbar, flow rate of E. faecium solution was 0.02 ml/h and stirring rate was 200 rpm. For mucoadhesive ability, Monolayer of HT-29 cells used as a colon cell and encapsulated cells were inoculated and incubated in 37℃, 5% CO2/95% air atmosphere for 1 h. Encapsulation efficiency of the encapsulation method used in this study was 98.2%. For mucoadhesive test, the concentration of inoculated E. faecium was 9.9×108 CFU/ml and the concentration of adhered E. faecium was 1.6×106 CFU/ml. In conclusion, encapsulation efficiency of extrusion method was high enough to be accepted for this study, however, alginate-Ca2+ microspheres revealed lower adhesive ability compared to expectation. Therefore, it needs further studies to increase adhesive ability with other polymers.
H2AX의 BRCA1 NLS domain과 BARD1 BRCT domain 각각과의 in vitro 상호 결합
배승희(Seunghee Bae),이선미(Sun-Mi Lee),김수미(Sumi Kim),최태부(Tae-Boo Choe),김차순(Cha Soon Kim),성기문(Ki-Moon Seong),진영우(Young-Woo Jin),안성관(Sungkwan An) 한국생물공학회 2009 KSBB Journal Vol.24 No.4
본 연구에서는 H2AX의 생리학적인 기능 및 분자세포 생물학적 기전 해석에 대한 보다 명확한 정보를 제시하고자, H2AX 관련 단백질들을 literature review 및 생물정보학적인 기술을 이용하여 최적의 결합 단백질체를 40개를 예측하고, 이들 가운데 상호작용 가능성이 높은 BRCA1 와 BARD1 단백질을 선별하여 in vitro 결합실험을 통해 이를 증명하였다. 이들 두 가지의 유전자를 발굴하여, 클로닝하였다. 클로닝된 유전자를 이용하여 두 가지 단백질을 발현 및 정제하였으며, 단백질들의 자체적인 구조에 의한 결합능력을 판단하기 위해 in vitro binding assay법을 실시하였다. 단백질의 구조적 안정과 비특이적 결합을 억제하는 detergent만이 포함된 상태에서, 구조학적 및 물리학적 상호 결합의 유무를 판정할 수 있게 하였으며, BRCA1과 BARD1은 모두 H2AX에 결합함을 확인하였다. 이런 실험 결과를 바탕으로 각각의 단백질에 대해 H2AX와의 최적 결합 부위를 알아내기 위해 각 유전자의 domain을 생물정 보학적으로 분석하였다. 이에 RING domain, NES, NLS 및 BRCT domain에 해당하는 유전자 부분을 새로 클로닝하여, 다시 in vitro 결합실험 및 실험결과에 대한 literature review를 통한 분석을 실시한 결과, H2AX는 BRCA1의 NLS, BARD1의 BRCT domain 부분과 결합하는 것을 확인하였다. H2AX에 대한 BRCA1과 BARD1과의 결합은 DNA repair에 있어 BRCA1의 NLS와 BARD1의 BRCT domain을 통해 H2AX foci의 관련 세포 신호전달 기전에 중요한 역할을 하여 전체적으로 genomic stability에 영향을 미칠 가능성이 농후할 것으로 사료된다. H2AX, a crucial component of chromatin, is implicated in DNA repair, cell cycle check point and tumor suppression. The aim of this study was to identify direct binding partners of H2AX to regulate cellular responses to above mechanisms. Literature reviews and bioinformatical tools were attempted intensively to find binding partners of H2AX, which resulted in identifying two potential proteins, breast cancer-1 (BRCA1) and BRCA1-associated RING domain 1 (BARD1). Although it has been reported in vivo that BRCA1 co-localizes with H2AX at the site of DNA damage, their biochemical mechanism for H2AX were however only known that the complex monoubiquitinates histone monomers, including unphosphorylated H2AX in vitro. Therefore, it is important to know whether the complex directly interacts with H2AX, and also which regions of these are specifically mediated for the interaction. Using in vitro GST pull-down assay, we present here that BRCA1 and BARD1 directly bind to H2AX. Moreover, through combinational approaches of domain analysis, fragment clonings and in vitro binding assay, we revealed molecular details of the BRCA1-H2AX and BARD1-H2AX complex. These data provide the potential evidence that each of the BRCA1 nuclear localization signal (NLS) and BARD1 BRCA1 C-terminal (BRCT) repeat domain is the novel mediator of H2AX recognition.
배수영(Su-Young Bae),권수미(Sumi Kwon) 한국교원대학교 뇌기반교육연구소 2020 Brain, Digital, & Learning Vol.10 No.4
The purpose of this study was to analyze the eye movements of pre-service elementary school teachers while reading music and playing the piano to find out the difference according to music reading ability. To this end, each subject was asked to read a 5-bar single line melody and a 5-bar melody in parallel motion through a computer monitor and play on the electronic piano at the same time. Meanwhile, the eye tracker attached to the bottom of the computer monitor detects the eye movements. The results were as follow: First, total fixation number, mean fixation duration, and total fixation duration were different between reading a single line melody and reading a melody in parallel motion. Second, there was no significant correlation between music reading ability and total fixation number and mean fixation duration. Third, music reading ability was influenced by the way of music reading approach. Based on these findings, the study suggests implications for developing music reading ability.
Lee, Hee Joong,Do, Jin Hwan,Bae, Sumi,Yang, Sanghwa,Zhang, Xianglon,Lee, Ahwon,Choi, Young Jin,Park, Dong Choon,Ahn, Woong Shick Science and Technology Letters 2011 MEDICAL ONCOLOGY -NORTHWOOD THEN BASINGSTOKE THEN Vol.28 No.1
<P>Glutathione peroxidase 3 (GPX3) is a member of glutathione peroxidase family, exerting one of the most important cellular defense mechanisms against stress signals, including oxidative damage. In this study, the expression of GPX3 mRNA and protein was analyzed for ovarian cancer tissues to test its applicability as a biomarker that can distinguish the four major histologic types of epithelial ovarian cancer. A public microarray dataset containing 99 ovarian cancer and 4 normal ovary samples was downloaded, and GPX3 mRNA expression was analyzed. The expression of GPX3 protein was measured by immunohistochemical staining in 40 epithelial ovarian cancer tissues, 10 for each of the serous, endometrioid, mucinous, and clear cell type. Histoscores were made from the immunohistostaining, and analysis of variance (ANOVA) was performed to quantitate the differences in protein level. Analysis of genomic dataset confirms a GPX3 overexpression in clear cell type ovarian adenocarcinoma compared with normal ovary and 3 other subtypes of epithelial ovarian cancer at mRNA level. GPX3 also shows the highest average antibody staining intensities in clear cell type ovarian adenocarcinomas over the other 3 types in immunostaining on tissue arrays. This is the first validation of GPX3 as a clear cell type-specific biomarker in ovarian cancer patients' tissues by immunostaining. GPX3 may serve as an important molecular marker for the diagnosis and molecular understanding of clear cell carcinoma of the ovary.</P>
Process Innovation Improves Trial Operation Efficiency
Choi, Yun Jung,Kim, Kyu-pyo,Park, Sumi,Park, MiYeon,Kim, Sulhwa,Kim, Younkyoung,Bae, Kyun-Seop,Beck, Sung-Ho,Choi, Ki-Eun,Chung, Jong Woo,Lim, Young–,Suk,Kim, Tae Won SAGE Publications 2016 Therapeutic innovation & regulatory science Vol.50 No.4
<P>Background: Despite the fact that unaddressed delays in clinical trial operation could severely compromise the overall effort invested, there seems to be a lack of concerted effort in reforming such delays. This study evaluated the composite effect of initiatives in reforming trial operation efficiency. Methods: A high-volume academic medical center in Korea has implemented various initiatives to improve the trial operation efficiency by expediting times from institutional review board (IRB) submission to approval, from IRB submission to trial open for subject enrollment, and from trial open to first patient-in. The initiatives include implementation of the protocol preliminary review, parallel processing of the clinical trial agreement review in line with the protocol submission to the IRB, and involvement of project manager for operational risk management. Times from IRB submission to approval, from IRB submission to trial open, and from trial open to first patient-in before and after implementation of initiatives were compared. Results: The median time required in IRB approval was meaningfully shorter in the postinitiative group (19 vs 14 days; P < .001). The median times from IRB submission to trial open for subject enrollment and from trial open to first patient-in were reduced significantly in the postinitiative group (trial open: 25 vs 18 days, P < .001; first patient-in: 111.5 vs 100 days, P = .014). Conclusions: The initiatives were effective in reforming trial operational efficiency. Additional studies to address the cause of operational delay and modifiable factors influencing subject enrollment are needed to further improve operational efficiency.</P>