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      • KCI등재

        Insight on the microbial activity and microbiome in partial nitrification systems: CuO nanoparticles impact under different pH levels

        Xiaojing Zhang,Nan Zhang,Yue Zhou,Robert Delatolla,Yali Song,Yongpeng Ma,Hongzhong Zhang 대한환경공학회 2020 Environmental Engineering Research Vol.25 No.6

        In this study, the effect of CuO nanoparticles (NPs) on partial nitrification (PN) process was investigated at various pH values. The short- and long-term experiments were carried out in six identical reactors, with or without CuO NPs, at pH values of 6.5, 8.0 and 10.0. The ammonia oxidation, reaction rates, copper content, and the microbial community were investigated. The results of this work suggested that CuO NPs inhibited the ammonia oxidation by aerobic ammonia-oxidizing bacteria (AOB), under both the acid and alkali conditions. AOB could resist the acid condition but was significantly suppressed when CuO NPs was fed, and the low pH did not aggravate the inhibition level. Almost all the bacteria lost bioactivity under the pH as 10.0, while anaerobic ammonia-oxidizing bacteria survived. The acid condition increased the Nitrosomonas relative abundance while the alkali condition lowered it. More than 60% of the supplied CuO NPs was discharged via effluent.

      • KCI등재

        Overexpression of the ABC transporter gene TsABCG11 increases cuticle lipids and abiotic stress tolerance in Arabidopsis

        Ningmei Chen,Buerbatu Song,Shuai Tang,Junqing He,Yijun Zhou,Jinchao Feng,Sha Shi,Xiaojing Xu 한국식물생명공학회 2018 Plant biotechnology reports Vol.12 No.5

        The cuticle, composed primarily of wax and cutin, covers most plant aerial surfaces and plays a vital role in interactions between plants and their environment. Some ATP-binding cassette G subfamily (ABCG) members are involved in cuticular lipid molecule exportation to outside in the plant surface. Thellungiella salsugineum, a relative of Arabidopsis thaliana with a heavy cuticle, has extreme stress tolerance. TsABCG11, an ABCG transporter was cloned (GenBank accession number JQ389853), and its structure was studied. qRT-PCR showed that TsABCG11 expression varied in different organs of T. salsugineum and was upregulated under ABA, NaCl, drought and cold conditions. The rosette leaves from 4-week-old TsABCG11 overexpressed (OE) Arabidopsis plants displayed lower rates of water loss and decreased chlorophyll-extracted rates compared to wild-type plants. TsABCG11-OE plants also exhibited significantly increased total cuticular wax and cutin monomer amounts, mainly due to prominent changes in the C29, C31, and C33 alkanes in the wax and C18:2 dioic in cutin monomers, respectively. TsABCG11-OE seedlings exhibit lower root growth inhibition under 100 mM of NaCl or 1 μM of ABA than the wild type. Four-week-old TsABCG11-OE plants exhibited higher photosynthetic rates and water-use efficiency under cold stress (4 °C) than control plants. These results indicate that TsABCG11 plays an important role in cuticle lipid exportation and is involved in abiotic stresses, probably having a close relationship with extreme stress tolerance in T. salsugineum.

      • KCI등재

        Dynamic measurements of hydrate based gas separation in cooled silica gel

        Mingjun Yang,Yongchen Song,Lanlan Jiang,Xiaojing Wang,Weiguo Liu,Yuechao Zhao,Yu Liu,Shanrong Wang 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.1

        Hydrate based gas separation is a promising method for carbon dioxide capture. The purpose of thisstudy is to analyze hydrates formation and dissociation characters when gas mixture flows throughcooled silica gel. The additives mixture (THF/SDS) was used to saturate the silica gel partly, and gasmixture (CO2/H2) was injected into it to form hydrates. Magnetic resonance imaging (MRI) images wereobtained using fast spin echo multi-slice pulse sequence. Hydrates saturations were calculatedquantitatively using MRI data. The experimental results showed that the optimal initial solutionsaturation was 34.2% in this investigation. The gas component was analyzed to assess the separationefficiency. For hydrates dissociation processes at 1 atmospheric pressure, CO2 concentrations increasedobviously. Half of the six cycles showed that more than 85.00 mol% CO2 contained in the capture gas, andthe lowest CO2 concentration was 64.83 mol%. Hydrate blockages appeared frequently, which restrictedthe contact of gas and solution and caused the incomplete transformations of residual solution tohydrates. It was a key restricted factor for hydrate based CO2 capture.

      • KCI등재

        Umbilical Cord Mesenchymal Stem Cell Treatment for Crohn’s Disease: A Randomized Controlled Clinical Trial

        ( Jian Zhang ),( Samei Lv ),( Xiaojing Liu ),( Bin Song ),( Liping Shi ) 대한간학회 2018 Gut and Liver Vol.12 No.1

        Background/Aims: Stem cell therapy has been applied to treat a variety of autoimmune diseases, including Crohn’s disease (CD), but few studies have examined the use of umbilical cord mesenchymal stem cells (UC-MSCs). This trial sought to investigate the efficacy and safety of UC-MSCs for the treatment of CD. Methods: Eighty-two patients who had been diagnosed with CD and had received steroid maintenance therapy for more than 6 months were included in this study. Forty-one patients were randomly selected to receive a total of four peripheral intravenous infusions of 1×10<sup>6</sup> UC-MSCs/kg, with one infusion per week. Patients were followed up for 12 months. The Crohn’s disease activity index (CDAI), Harvey-Bradshaw index (HBI), and corticosteroid dosage were assessed. Results: Twelve months after treatment, the CDAI, HBI, and corticosteroid dosage had decreased by 62.5±23.2, 3.4±1.2, and 4.2±0.84 mg/day, respectively, in the UC-MSC group and by 23.6±12.4, 1.2±0.58, and 1.2±0.35 mg/day, respectively, in the control group (p<0.01, p<0.05, and p<0.05 for UC-MSC vs control, respectively). Four patients developed a fever after cell infusion. No serious adverse events were observed. Conclusions: UC-MSCs were effective in the treatment of CD and produced mild side effects. (Gut Liver 2018;12:73-78)

      • KCI등재

        Application of Biophysical Properties of Meridians in the Visualization of Pericardium Meridian

        Xiong Feng,Xu Ruimin,Li Tongju,Wang Jinyu,Hu Qingchuan,Song Xiaojing,Wang Guangjun,Su Huanhuan,Jia Shuyong,Wang Shuyou,Zhu Zongxiang,Zhang Weibo 사단법인약침학회 2023 Journal of Acupuncture & Meridian Studies Vol.16 No.3

        Background: The biophysical properties of the meridian system, an important concept of traditional Chinese medicine, include low impedance, resounding voice, and high acoustic conductance, all of which are helpful for elucidating the essence of meridians. Objectives: To visualize the human pericardium meridian (PC) based on the resounding voice property of meridians. Methods: Visualization of the PC was performed by injection of fluorescein sodium at the PC6 acupoint (Neiguan) on the PC. Before injection, percussion active points (PAPs) were identified by the virtue of their resounding voice properties. After injection, the trajectories of fluorescein migration throughout the body surface were recorded and analyzed. The distribution of fluorescein in the tissue was further studied using cross-sections of hind limbs of mini-pigs, in which fluorescein was injected into low impedance points. Results: The identified PAP lines were colocalized with PC. Following intradermal fluorescein injection, 1-3 fluorescent lines, which were unrelated to the arm veins, were observed in 7 of 10 participants; 85.4% of fluorescent signals were coincident with PAPs and their intensity had a negative correlation with the body mass index (r = –0.56, p = 0.045). Cross-sections showed a Y-shaped fluorescence pattern where the two migration lines on the surface were the two vertices of the “Y.” Conclusion: The trajectories of fluorescein in the body are suggestive of the anatomical structure of meridians. The PC is related to the deep horizontal interstitial channels that connect to the body surface through vertical interstitial spaces. These biophysical properties and techniques for meridian visualization are valuable for revealing the anatomical structure of meridians.

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