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ShuQin Liu,Song Lian,YunZhou Yang,ChunZheng Fu,HongYing Ma,ZhiYao Xiong,Yao Ling,ChunJiang Zhao 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.12
Objective: An experiment was conducted to study the association between the single nucleotide polymorphisms (SNPs) in 5'-untranslated regions (5'-UTR) of equine chorionic gonadotropin (eCG) genes and the serum eCG levels. Methods: SNPs in 5'-UTR of eCG genes were screened across 10 horse breeds, including 7 Chinese indigenous breeds and 3 imported breeds using iPLEX chemistry, and the association between the serum eCG levels of 174 pregnant Da’an mares and their serum eCG levels (determined with ELISA) was analyzed. Results: Four SNPs were identified in the 5'-UTR of the eCGα gene, and one of them was unique in the indigenous breeds. There were 2 SNPs detected at the 5’ end of the eCGβ subunit gene, and one of them was only found in the Chinese breeds. The SNP g.39948246T>C at the 5'-UTR of eCGα was associated significantly with eCG levels of 75-day pregnant mare serum (p<0.05) in Da’an mares. Prediction analysis on binding sites of transcription factors showed that the g.39948246T>C mutation causes appearance of the specific binding site of hepatocyte nuclear factor 3 forkhead homolog 2 (HFH-2), which is a transcriptional repressor belonging to the forkhead protein family of transcription factors. Conclusion: The SNP g.39948246T>C at the 5'-UTR of eCGα is associated with eCG levels of 75-day pregnant mare serum (p<0.05).
Lina Xiong,Shuqin Mao,Baiyi Lu,Jiajia Yang,Fei Zhou,Yinzhou Hu,Yirong Jiang,Canxi Shen,Yajing Zhao 한국식품영양과학회 2016 Journal of medicinal food Vol.19 No.1
Osmanthus fragrans flower extract (OFE) is an organic extract from O. fragrans flower, which exhibits neuroprotective, free radical scavenging, and antioxidant effects. Therefore, the protective effect of OFE and acteoside against aging was studied. An aging ICR mouse model was established by chronically administering d-galactose (250 mg/kg) for 8 weeks. d-galactose induced spatial learning and memory impairments that were successfully inhibited by OFE and acteoside, which could shorten escape latency, improve platform crossing times, and increase zone time. The antioxidant potential of OFE and acteoside in vivo was evaluated by estimating the following: activities of antioxidant enzymes, such as glutathione peroxidase and aging-related enzyme, particularly monoamine oxidase; contents of lipid peroxidation methane dicarboxylic aldehyde, advanced glycation end products, and 8-hydroxy-2'-deoxyguanosine (a DNA damage product); and levels of nuclear factor-erythroid 2-related factor 2. OFE and acteoside also inhibited d-galactose-induced neurological aging by suppressing the increase in glial fibrillary acidic protein and neurotrophin-3. Considering the dose-dependent protective effects of OFE and acteoside, we concluded that OFE, rich in acteoside, was a good source of natural antiaging compounds.
Chunyu Zhu,Fangliang Zheng,Sugai Yin,Hongsheng Liu,Yan Liu,Maosheng Zhang,Shuqin Xiong 한국유전학회 2014 Genes & Genomics Vol.36 No.6
The baculovirus ChaB proteins are conservedin all completely sequenced Lepidopteran NPVs and areannotated as putative DNA binding proteins. In our previousstudy, ORF51, a ChaB homologue from Helicoverpaarmigera single nucleocapsid nucleopolyhedrovirus(HearNPV) was found to be involved in budded virusproduction and DNA replication. In the present study, wecharacterized ORF52 (Ha52), the other ChaB homologousgene in HearNPV. 50-RACE revealed that Ha52 was transcribedfrom a conventional late promoter transcriptionalinitiator motif (TAAG) located 25 nucleotides upstream ofATG. Expression analysis demonstrated that HA52 proteinwas expressed from 48 to 96 h post infection. Western blotanalysis of virions from both budded viruses (BVs) andocclusion-derived viruses (ODVs) indicated that HA52 wasa structural component of nucleocapsid from ODV. Tostudy the function of Ha52 in the life cycle of HearNPV,Ha52-knockout and Ha52-restored viruses were generatedby the Bac-to-Bac system. Growth curve analyses showedthat the level of BV production in cells infected with Ha52null virus was similar to those infected with wild-typebacmid derived virus. However, bioassay showed thatdeletion of Ha52 significantly decreased the per os infectivityof HearNPV. Taken together, our results indicatedthat, unlike the previously characterized baculovirual ChaBgenes, Ha52 may be involved in HearNPV per os infection.