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Generation of an scFv-type Quenchbody Against HER2 for Sensitive Detection of Human Breast Cancer
Rizi Shima Yousefi,김선희,이정훈,정희진 한국생물공학회 2023 KSBB Journal Vol.38 No.1
The advanced clinical stage of breast cancer causes a lower survival rate even though the survival rate in the early stages is relatively high. Thus, the development of a rapid and easy-to-access diagnostic method is needed. In this study, a single chain Fv (scFv)-type antibody fragment that binds to HER2, a transmembrane protein overexpressed on breast cancer cells, was generated. The scFv was converted to a Quenchbody by site-specifically labeling the scFv using fluorescent dye through a maleimide-thiol reaction. The Quenchbody could detect a picomolar order of HER2 in a few minutes, and its signal-to-background ratio of fluorescence intensity was higher than a fluorescence scFv generated through a conventional fluorescent dye-conjugating method. The Quenchbody can be used as a probe for detecting and imaging of breast cancer cells for point-of-care diagnosis of breast cancer.
Mohammad Yousefi,Shima Azizi,Seyed Mohsen Peyghambarzadeh,Zoha Azizi 한국화학공학회 2021 Korean Journal of Chemical Engineering Vol.38 No.4
The solubility of ethylene in N-methyl-2-pyrrolidone (NMP) was evaluated at different temperatures, including 278.2, 298.2, and 328.2 K, and different pressures in an experimental pressure decaying setup. The kinetic and equilibrium results were obtained for pure gas absorption. Henry’s law constants were calculated at different temperatures. Eventually, thermodynamic modeling was done using Peng Robinson equation of state (PR-EOS) and UNIQUAC activity coefficient model. The binary interaction parameters, 12, 21, were adjusted and optimized. Regarding the values obtained for binary interaction parameters, it was concluded that this solution has non-ideal behavior. Indeed, because of its low prediction error (3-11%), it was concluded that the correlated thermodynamic model could accurately predict the experimental data.
Optimization of vitamin B12 nano-emulsification and encapsulation using spontaneous emulsification
Shabnam Karbalaei-Saleh,Shima Yousefi,Masoud Honarvar 한국식품과학회 2024 Food Science and Biotechnology Vol.33 No.2
In this study, the use of low-energy methods for nanoemulsification of vitamin B12 was investigated to protect this bioactive substance. The effects of sunflower oil concentrations (4–8%), Tween 80 (8–16%), and vitamin B12 (5–15%) on the physicochemical properties of B12 nanoemulsion were evaluated using response surface methodology (RSM). The results indicated that the quadratic model was the most fitting model for experimental data. Optimization revealed that the optimal formulation contained 6.5% sunflower oil, 9.6% Tween 80, and 13% vitamin B12, resulting in maximum efficiency, viscosity, and vitamin B12 content, as well as minimum pH, turbidity, p-Anisidine index, particle size, and polydispersity index (PDI). Under optimal conditions, pH, viscosity, turbidity, efficiency, vitamin B12, p-Anisidine index, PDI, and particle size were 7.24, 17.0024 cp, 2.19, 51.98%, 5.54 ppm, 0.01, 0.34, and 322 nm, respectively. This study highlights the effectiveness of spontaneous emulsification as a carrier for the encapsulation of bioactive compounds.