Purification and Characterization of an Antioxidant Protein from Fertilized Eggs

Shaohua Yang,Zhengjiang Tang,ShanShan Tang,Tingfang Zhang,Fei Tang,Yu Wu,Ying Wang,Lulu Wang,Guoqing Liu 한국축산식품학회 2016 한국축산식품학회지 Vol.36 No.6

Free radicals may attack cells or tissue, leading to chronic diseases, and antioxidant consumption is potentially useful for removing free radicals. Egg proteins may be used as potential sources of antioxidant considering their ability of scavenging free radicals to apply for food or cosmetics industry. In this study, we obtained a natural antioxidant protein from fertilized eggs, which was a dietary supplement in some Asian countries. Meanwhile, antioxidant activities of these proteins were evaluated using different oxidation systems. With increasing incubation time, the antioxidant activity of these proteins increased during 15 d of incubation. The samples on day 15 were performed for isolation of antioxidant protein. The protein, named P4-1 (MW, 45 kDa), was isolated and purified by consecutive chromatographic methods. P4-1 contained 17 amino acids, which was determined by liquid chromatography-mass spectrometry and Amino Acid Analyzer. Moreover, the amino acid sequence was highly similar to that of ovalbumin. Differential scanning calorimetry showed that the denaturation temperature of P4-1 was 57.16ºC. Furthermore, P4-1 suggested high oxygen radical-absorbance activity in ·OH assays, and its antioxidant activity was stable at 30-50ºC in acidic and neutral pH. Thus, these results revealed that P4-1 may be a potential resource as a natural antioxidant.

Purification and Characterization of an Antioxidant Protein from Fertilized Eggs

Yang, Shaohua,Tang, Zhengjiang,Tang, ShanShan,Zhang, Tingfang,Tang, Fei,Wu, Yu,Wang, Ying,Wang, Lu Lu,Liu, Guoqing Korean Society for Food Science of Animal Resource 2016 한국축산식품학회지 Vol.36 No.6

Free radicals may attack cells or tissue, leading to chronic diseases, and antioxidant consumption is potentially useful for removing free radicals. Egg proteins may be used as potential sources of antioxidant considering their ability of scavenging free radicals to apply for food or cosmetics industry. In this study, we obtained a natural antioxidant protein from fertilized eggs, which was a dietary supplement in some Asian countries. Meanwhile, antioxidant activities of these proteins were evaluated using different oxidation systems. With increasing incubation time, the antioxidant activity of these proteins increased during 15 d of incubation. The samples on day 15 were performed for isolation of antioxidant protein. The protein, named P4-1 (MW, 45 kDa), was isolated and purified by consecutive chromatographic methods. P4-1 contained 17 amino acids, which was determined by liquid chromatography-mass spectrometry and Amino Acid Analyzer. Moreover, the amino acid sequence was highly similar to that of ovalbumin. Differential scanning calorimetry showed that the denaturation temperature of P4-1 was $57.16^{\circ}C$. Furthermore, P4-1 suggested high oxygen radical-absorbance activity in ${\cdot}OH$ assays, and its antioxidant activity was stable at $30-50^{\circ}C$ in acidic and neutral pH. Thus, these results revealed that P4-1 may be a potential resource as a natural antioxidant.

Purification and Identification of a Natural Antioxidant Protein from Fertilized Eggs

Shaohua Yang,Lulu Wang,Ying Wang,Xiaoqian Ou,Zhaoyuan Shi,Chongchong Lu,Wei Wang,Guoqing Liu 한국축산식품학회 2017 한국축산식품학회지 Vol.37 No.5

Fertilized hen eggs are rich in a variety of bioactive ingredients. In this study, we aimed to obtain an antioxidant protein from fertilized eggs and the radical scavenging abilities on 1, 1- diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical (•OH), superoxide anion (O2-•) were used to evaluate the antioxidant activity of the purified protein. During 20 d of incubation, the radical scavenging ability of protein extracted from fertilized eggs exhibited significantly differences and the protein on day 16 showed higher antioxidant capacity. Based on this, the antioxidant protein of the samples on day 16 were isolated for the follow-up study. With a molecular weight 43.22 kDa, the antioxidant protein was purified by Diethylaminoethyl cellulose -52 (DEAE-52) column and Sephadex G-100. The LC-MS analysis showed that the purified protein molecular weight was 43.22 kDa, named D2-S. The sequence of amino acids was highly similar to ovalbumin and the coverage reached to 84%. The purified protein showed a radical scavenging rate of 52.34±3.27% on DPPH and 63.49±0.25% on •OH, respectively. Furthermore, the C-terminal amino acid sequence was NAVLFFGRCVSP, which was consistent with the sequence of ovabumin. These results here indicated that purified protein may be a potential resource as a natural antioxidant.

Adjustable and extensible hexagonal acoustic metamaterial cell combining multiple parallel Helmholtz resonators with optional apertures

Fei Yang,Shaohua Bi,Xinmin Shen,Xiaocui Yang,Xinqing Wang,Xiangpo Zhang,Jinhong Xue,Xiaonan Zhang,Qin Yin,Juying Dai,Wenqiang Peng,Jingwei Zhu,Junhong Ren 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.12

To prevent noise pollution, a hexagonal acoustic metamaterial cell combining multiple parallel Helmholtz resonators with optional apertures is proposed. There were 6 trapezoidal chambers and 6 triangular chambers, and each front panel had 6 different apertures, which meant that there were 6 12 = 2176782336 possible permutations. The distribution of sound pressures obtained by acoustic finite element simulation revealed the acoustic absorption mechanism, which provided effective guidance to alter the absorption capacity. For certain scenarios, the acoustic absorption performance was optimized by the joint combination of artificial neural network and acoustic finite element simulation. Through manufacturing and testing the sample, actual average acoustic absorption coefficients were achieved at 0.6733, 0.7296, 0.8785 and 0.7065 for the target frequency ranges 350-950 Hz, 400-1000 Hz, 500-800 Hz and 350-700 Hz, respectively, with total thickness 40 mm. The tunable acoustic absorption property proved that the hexagonal acoustic metamaterial cell was appropriate for noise reduction with variable frequency ranges.

Coupling natural and human processes to simulate changes in the water environment in the Dianchi Lake basin, China

Guilin Wang,Kun Yang,Yang Yang,Shaohua Zhang 한국자원공학회 2017 Geosystem engineering Vol.20 No.4

This study proposes a comprehensive simulation model that couples natural and human processes that have traditionally been simulated and analyzed independently. This model was established to support a risk assessment associated with natural disasters and social emergencies. In this paper, a driving mechanism was refined to reflect changes in the water environment and to understand the natural process of non-point source pollution formation in the Dianchi Lake basin. The land use and land cover model, incorporating the changes from the human activities and the natural process, was constructed to understand how humans affect the land use and land cover in the Dianchi Lake basin. Moreover, this paper studies the effects of land use and land cover changes on the non-point pollution and the urban non-point pollution with the SWAT and SWMM models. The land use and land cover changes, especially the growth and spread of the urban area, are changing the structure of the ecosystem and are degenerating the functions of the ecosystem. Ultimately, this study comprehensively simulated natural and human processes to analyze the effects of the human activities on water environment.

Multiple damages detection in beam based approximate waveform capacity dimension

Yang, Zhibo,Chen, Xuefeng,Tian, Shaohua,He, Zhengjia Techno-Press 2012 Structural Engineering and Mechanics, An Int'l Jou Vol.41 No.5

A number of mode shape-based structure damage identification methods have been verified by numerical simulations or experiments for on-line structure health monitoring (SHM). However, many of them need a baseline mode shape generated by the healthy structure serving as a reference to identify damages. Otherwise these methods can hardly perform well when multiple cracks conditions occur. So it is important to solve the problems above. By aid of the fractal dimension method (FD), Qiao and Wang proposed a generalized fractal dimension (GFD) to detect the delamination damage. As a modification of GFD, Qiao and Cao proposed the approximate waveform capacity dimension (AWCD) technique to simplify the calculation of fractal and overcome the false peak appearing in the high mode shapes. Based on their valued work, this paper combined and applied the AWCD method and curvature mode shape data to detect multiple damages in beam. In the end, the identification properties of the AWCD for multiple damages have been verified by groups of Monte Carlo simulations and experiments.

Damage detection using the improved Kullback-Leibler divergence

Shaohua Tian,Xuefeng Chen,Zhibo Yang,Zhengjia He,Xingwu Zhang 국제구조공학회 2013 Structural Engineering and Mechanics, An Int'l Jou Vol.48 No.3

Structural health monitoring is crucial to maintain the structural performance safely. Moreover, the Kullback-Leibler divergence (KLD) is applied usually to asset the similarity between different probability density functions in the pattern recognition. In this study, the KLD is employed to detect the damage. However the asymmetry of the KLD is a shortcoming for the damage detection, to overcoming this shortcoming, two other divergences and one statistic distribution are proposed. Then the damage identification by the KLD and its three descriptions from the symmetric point of view is investigated. In order to improve the reliability and accuracy of the four divergences, the gapped smoothing method (GSM) is adopted. On the basis of the damage index approach, the new damage index (DI) for detect damage more accurately based on the four divergences is developed. In the last, the grey relational coefficient and hypothesis test (GRCHT) is utilized to obtain the more precise damage identification results. Finally, a clear remarkable improvement can be observed. To demonstrate the feasibility and accuracy of the proposed method, examples of an isotropic beam with different damage scenarios are employed so as to check the present approaches numerically. The final results show that the developed approach successfully located the damaged region in all cases effect and accurately.

Damage detection using the improved Kullback-Leibler divergence

Tian, Shaohua,Chen, Xuefeng,Yang, Zhibo,He, Zhengjia,Zhang, Xingwu Techno-Press 2013 Structural Engineering and Mechanics, An Int'l Jou Vol.48 No.3

Structural health monitoring is crucial to maintain the structural performance safely. Moreover, the Kullback-Leibler divergence (KLD) is applied usually to asset the similarity between different probability density functions in the pattern recognition. In this study, the KLD is employed to detect the damage. However the asymmetry of the KLD is a shortcoming for the damage detection, to overcoming this shortcoming, two other divergences and one statistic distribution are proposed. Then the damage identification by the KLD and its three descriptions from the symmetric point of view is investigated. In order to improve the reliability and accuracy of the four divergences, the gapped smoothing method (GSM) is adopted. On the basis of the damage index approach, the new damage index (DI) for detect damage more accurately based on the four divergences is developed. In the last, the grey relational coefficient and hypothesis test (GRCHT) is utilized to obtain the more precise damage identification results. Finally, a clear remarkable improvement can be observed. To demonstrate the feasibility and accuracy of the proposed method, examples of an isotropic beam with different damage scenarios are employed so as to check the present approaches numerically. The final results show that the developed approach successfully located the damaged region in all cases effect and accurately.

Multiple damages detection in beam based approximate waveform capacity dimension

Zhibo Yang,Xuefeng Chen,Shaohua Tian,Zhengjia He 국제구조공학회 2012 Structural Engineering and Mechanics, An Int'l Jou Vol.41 No.5

A number of mode shape-based structure damage identification methods have been verified by numerical simulations or experiments for on-line structure health monitoring (SHM). However, many of them need a baseline mode shape generated by the healthy structure serving as a reference to identify damages. Otherwise these methods can hardly perform well when multiple cracks conditions occur. So it is important to solve the problems above. By aid of the fractal dimension method (FD), Qiao and Wang proposed a generalized fractal dimension (GFD) to detect the delamination damage. As a modification of GFD, Qiao and Cao proposed the approximate waveform capacity dimension (AWCD) technique to simplify the calculation of fractal and overcome the false peak appearing in the high mode shapes. Based on their valued work, this paper combined and applied the AWCD method and curvature mode shape data to detect multiple damages in beam. In the end, the identification properties of the AWCD for multiple damages have been verified by groups of Monte Carlo simulations and experiments.

Industrial test on coal re-burning at a 600MW utility boiler and NOx reduction

Penghua Qiu,Shaohua Wu,Shaozeng Sun,Hui Liu,Longbin Yang,Guozhong Wang 한국화학공학회 2007 Korean Journal of Chemical Engineering Vol.24 No.4

research conducts a series of industrial tests on coal reburning of a 600 MW pulverized coal boilerfiring lignite, which is one part of a coal reburning demonstration project. When running steadily under 60 MW load,the boiler has an average NOx emission of 274 mg/m3 (O2 content in flue gas is converted to 6% ), the NOx emissionis reduced by 65.36% . In the meanwhile, loss of ignition (LOI) under coal reburning rarely increases. Three operationconditions - traditional air feeding, air staging and coal reburning - are realized, respectively, during the industrial tests,and the results indicate that coal reburning has the lowest NOx emission, while the traditional air feeding has the highestNOx x controlcan reach.