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Effect of Germination and Heating on Phytase Activity in Cereal Seeds
Ma, Xi,Shan, Anshan Asian Australasian Association of Animal Productio 2002 Animal Bioscience Vol.15 No.7
The effect of germination on phytase activity in wheat NEAU123, triticale5305 and rye2 was studied in the present study. Germination significantly increased phytase activity by 2.04 times for wheat NEAU123 (3 d), 1.82 times for triticale 5305 (1 d) and 2.45 times for rye2 (1 d), respectively. It was safe for phytase in fresh malts kilned for 2 h at $40^{\circ}C$. Phytase in cereal seeds had strong heat stability. There was no loss of phytase activity in cereal seeds heated at $70^{\circ}C$ for 1 h, a little loss (${\leq}$5.46%) at $80^{\circ}C$ or $90^{\circ}C$. Even heated at $100^{\circ}C$, the phytase activity in wheat NEAU123, triticale5305 and rye2 remained 89.47%, 86.44% and 104.64%, respectively.
Ma, Deying,Liu, Yuqin,Liu, Shengwang,Li, Qundao,Shan, Anshan Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.9
The experiment was conducted to evaluate the effects of Ligustrum lucidum (LL) and Schisandra chinensis (SC) on the growth, antioxidative metabolism and immunity of laying strain male chicks. The results showed that diets supplemented with 1% of either LL or SC had no effects on the growth performance of chicks compared with the control. Furthermore, both LL and SC significantly reduced malondialdehyde (MDA) concentration of serum and heart of chicks (p<0.05). In addition, superoxide dismutase (SOD) activity of serum of the birds was significantly elevated by supplementation with SC (p<0.05). Glutathione reductase (GR) activity of heart and serum of the birds was significantly elevated by supplementation with LL or SC (p<0.05). LL supplementation significantly elevated antibody values against Newcastle Disease virus (NDV)(p<0.05) and lymphoblastogenesis (p<0.05) of the birds. The results suggest that diets supplemented with 1% of either LL or SC may improve immune function and antioxidant status of chicks.
Yu-Yang Chi,Jing-Lin Shen,Jing Zhang,Anshan Shan,Shu-Ling Niu,Chang-Hai Zhou,이홍구,Yong-Cheng Jin 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.4
The aim of this study is to reveal the relation among villin 2, Wnt/β-catenin, andadipogenesis by adding appropriate lithium chloride (LiCl). The study comprises three parts: theselection of LiCl concentration, the effect of LiCl on adipocyte differentiation during and afterdifferentiation induction. By comprehensively analyzing the results of the experiments, we proved thatLiCl can inhibit adipocyte differentiation and enhance villin 2 and β-catenin expressions not only duringdifferentiation induction but also after it. Moreover, villin 2 has a significant impact on β-catenin. Wesuggest that villin 2 may participate in Wnt/β-catenin signaling.
Li Feng,Yang Chunxu,Xie Yingjie,Gao Xiang,Zhang Yuanyuan,Ning Hangyi,Liu Guangtao,Chen Zhihui,Shan Anshan 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.8
Objective: The objectives of this study were to evaluate the effects of daily feed intake during the laying period on embryonic myogenic differentiation 1 (MYOD1), myogenic factor 5 (MYF5), and myogenic factor 6 (MYF6) gene expression in genetically fat and lean lines of chickens. Methods: An experiment in a 2×2 factorial design was conducted with two dietary intake levels (100% and 75% of nutrition recommendation) and two broiler chicken lines (fat and lean). Two lines of hens (n = 384 for each line) at 23th week of age were randomly divided into 4 treatments with 12 replicates of 16 birds. The experiment started at 27th week of age (5% egg rate) and ended at 54th week of age. Hatched eggs from the medium laying period were collected. Real time polymerase chain reaction analysis was used to analyse the MYOD1, MYF5, and MYF6 mRNA levels of E7, E9, E11, E13, and E15 body tissues and E17, E19, and E21 chest and thigh muscle samples. Results: The results indicated that there were significant effects of line, dietary intake, and interactions between them on MYOD1, MYF5, and MYF6 gene mRNA expression levels in embryonic tissues. Low daily feed intake did not change the expression trend of MYOD1 mRNA in either line, but changed the peak values, especially in lean line. Low daily feed intake altered the trend in MYF5 mRNA expression level in both lines and apparently delayed its onset. There was no apparent effect of low daily feed intake on the trends of MYF6 mRNA expression levels in either line, but it significantly changed the values on many embryonic days. Conclusion: Maternal nutrient restriction affects myogenesis and is manifested in the expression of embryonic MYOD1, MYF5, and MYF6 genes. Long term selection for fat deposition in broiler chickens changes the pattern and intensity of myogenesis. Objective: The objectives of this study were to evaluate the effects of daily feed intake during the laying period on embryonic myogenic differentiation 1 (<i>MYOD1</i>), myogenic factor 5 (<i>MYF5</i>), and myogenic factor 6 (<i>MYF6</i>) gene expression in genetically fat and lean lines of chickens.Methods: An experiment in a 2×2 factorial design was conducted with two dietary intake levels (100% and 75% of nutrition recommendation) and two broiler chicken lines (fat and lean). Two lines of hens (n = 384 for each line) at 23th week of age were randomly divided into 4 treatments with 12 replicates of 16 birds. The experiment started at 27th week of age (5% egg rate) and ended at 54th week of age. Hatched eggs from the medium laying period were collected. Real time polymerase chain reaction analysis was used to analyse the <i>MYOD1</i>, <i>MYF5</i>, and <i>MYF6</i> mRNA levels of E7, E9, E11, E13, and E15 body tissues and E17, E19, and E21 chest and thigh muscle samples.Results: The results indicated that there were significant effects of line, dietary intake, and interactions between them on <i>MYOD1</i>, <i>MYF5</i>, and <i>MYF6</i> gene mRNA expression levels in embryonic tissues. Low daily feed intake did not change the expression trend of <i>MYOD1</i> mRNA in either line, but changed the peak values, especially in lean line. Low daily feed intake altered the trend in <i>MYF5</i> mRNA expression level in both lines and apparently delayed its onset. There was no apparent effect of low daily feed intake on the trends of <i>MYF6</i> mRNA expression levels in either line, but it significantly changed the values on many embryonic days.Conclusion: Maternal nutrient restriction affects myogenesis and is manifested in the expression of embryonic <i>MYOD1</i>, <i>MYF5</i>, and <i>MYF6</i> genes. Long term selection for fat deposition in broiler chickens changes the pattern and intensity of myogenesis.