Supercritical extraction of decellularized extracellular matrix from porcine adipose tissue as regeneration therapeutics

Seungwon Chung,Hana Kwon,Namsoo Peter Kim 대한미용의학회 2019 대한미용의학회지 Vol.3 No.2

Background: Extracellular matrix (ECM) has been broadly applied and shown great promise in medical applications. ECM products should be used after decellularization and purification. Supercritical carbon dioxide treatment is of particular interest for purifying ECM due to its medical availability and rapid process speed. However, it is not fully researched for treatment of biomaterials for tissue engineering. Therefore, we investigated the optimal conditions of supercritical carbon dioxide processing at different extracting parameters in porcine adipose tissue. Objective: We aimed to identify the optimal supercritical extracting conditions to produce non-cytotoxic and sterile decellularized extracellular matrix (DE-ECM) for regeneration therapeutics. Methods: The three-day dual treatment including enzymatic decellularization and supercritical fluid extraction of pork adipose tissue was performed. Two protocols using different extracting parameters were applied to evaluate the influence of extracting pressure and temperature on the extraction yield, DNA concentration, and remaining collagen in product. Results: Yield rate increased when high temperature or pressure was applied and pre-enzyme treatment had higher yield rate percent than pre-supercritical processing. Nearly 90% DNA was removed from the pre-enzyme sample when extracted at 3.04×10 7 Pa and 30°C±5°C. The pre-enzyme process had efficient extracting ability at each temperature and pressure and the remaining collagen steadily decreased with increase in extracting pressure and temperature. At the lowest temperature (20°C±5°C) and pressure (1.01×10 7 Pa), remaining collagen was 75.74%±1.83%. Supercritical extraction technology can produce DE-ECM eliminating DNA content efficiently and the remaining proper collagen amount successfully. Conclusion: This study evaluated the feasibility of utilizing supercritical extraction technology in bio-materials and was proven to be is successful. Through controlling the extracting pressure and temperature, this technology has a potential for DE-ECM mass production, which can be useful as tissue regeneration therapeutics as well new drug delivery paradigm.

Sensitivity Analysis on a Vegetation Parameter for Evaluation of Phenology Representation of the Noah-MP Model

Seungwon Chung,Seungbum Hong,Seon Ki Park 한국기상학회 2013 한국기상학회 학술대회 논문집 Vol.2013 No.10

Visual Perception in Autism Spectrum Disorder: A Review of Neuroimaging Studies

Seungwon Chung,Jung-Woo Son 대한소아청소년정신의학회 2020 소아청소년정신의학 Vol.31 No.3

Although autism spectrum disorder (ASD) is a neurodevelopmental disorder characterized by social impairments, patients with ASD frequently manifest atypical sensory behaviors. Recently, atypical sensory perception in ASD has received much attention, yet little is known about its cause or neurobiology. Herein, we review the findings from neuroimaging studies related to visual perception in ASD. Specifically, we examined the neural underpinnings of visual detection, motion perception, and face processing in ASD. Results from neuroimaging studies indicate that atypical visual perception in ASD may be influenced by attention or higher order cognitive mechanisms, and atypical face perception may be affected by disrupted social brain network. However, there is considerable evidence for atypical early visual processing in ASD. It is likely that visual perceptual abnormalities are independent of deficits of social functions or cognition. Importantly, atypical visual perception in ASD may enhance difficulties in dealing with complex and subtle social stimuli, or improve outstanding abilities in certain fields in individuals with Savant syndrome. Thus, future research is required to elucidate the characteristics and neurobiology of autistic visual perception to effectively apply these findings in the interventions of ASD.

자폐스펙트럼장애 환자에서의 인지적 공감 및 정서적 공감의 신경 상관물

정승원(Seungwon Chung),손정우(Jung-Woo Son),이승복(Seungbok Lee),김혜리(Hei-Rhee Ghim),이상익(Sang-Ick Lee),신철진(Chul-Jin Shin),김시경(Siekyeong Kim),주가원(Gawon Ju),최상철(Sang Cheol Choi),김양렬(Yang Yeol Kim),구영진(Young Jin K 대한소아청소년정신의학회 2016 소아청소년정신의학 Vol.27 No.3

Objectives: Individuals with autism spectrum disorder (ASD) are considered to have problems with empathy. It has recently been suggested that there are two systems for empathy; cognitive and emotional. We aimed to investigate the neural response to cognitive and emotional empathy and elucidate the neurobiological aspects of empathy in patients with ASD. Methods: We recruited patients with ASD (N=17, ASD group) and healthy controls (HC) (N=22, HC group) for an functional magnetic resonance imaging study. All of the subjects were scanned while performing cognitive and emotional empathy tasks. The differences in brain activation between the groups were assessed by contrasting their neural activity during the tasks. Results: During both tasks, the ASD group showed greater neural activities in the bilateral occipital area compared to the HC group. The ASD group showed more activation in the bilateral precunei only during the emotional empathy task. No brain regions were more activated in the HC group than in the ASD group during the cognitive empathy task. While performing the emotional empathy task, the HC group exhibited greater neural activities in the left middle frontal gyrus and right anterior cingulate gyrus than the ASD group. Conclusion: This study showed that the brain regions associated with cognitive and emotional empathy in ASD patients differed from those in healthy individuals. The results of this study suggest that individuals with ASD might have defects both in cognitive empathy and in emotional empathy.

Antibacterial Effects of Vulgarone B from Artemisia iwayomogi Alone and in Combination with Oxacillin

Eun Young Chung,Youn Hee Byun,신은주,Ha Sook Chung,Yeon Hee Lee,Seungwon Shin 대한약학회 2009 Archives of Pharmacal Research Vol.32 No.12

The antibacterial activities of vulgarone B, a component of Artemisia iwayomogi essential oil, were evaluated against some antibiotic-susceptible and -resistant human pathogens. Moreover, the effects of combining antibiotics, such as oxacillin, with vulgarone B were determined in this study. Significant inhibitory activities of Artemisia oils against antibiotic-susceptible and -resistant bacteria were confirmed by broth microdilution methods. The effects of vulgarone B on bacterial morphology and DNA were observed by scanning electron microscope and electrophoresis, respectively. In checkerboard microtiter tests, vulgarone B and A. iwayomogi oil combined with oxacillin resulted in synergism, or additive effects. Moreover, the safety of Artemisia oil and vulgarone B were confirmed in vivo. Both vulgarone B and the essential oil fraction of A. iwayomogi showed significant inhibitory activities against strains of antibioticsusceptible and -resistant bacteria. The oils showed synergism or additive effects when combined with oxacillin against two strains of Staphylococcus aureus. The antibiotic mechanism involved might be related to DNA cleavage. Thus, vulgarone B and the essential oil fraction of A. iwayomogi may be promising candidates for a safe, effective, natural agent active against antibiotic-resistant S. aureus, especially when combined with oxacillin.

대화형 비디오 서비스를 위한 CORBA 기반의 분산 플랫폼

정형석(Hyungseok Chung),정광수(Kwangsue Chung),손승원(Seungwon Son) 한국정보과학회 1997 한국정보과학회 학술발표논문집 Vol.1997 No.4

Animal Sounds Classification Scheme Based on Multi-Feature Network with Mixed Datasets

( Chung-il Kim ),( Yongjang Cho ),( Seungwon Jung ),( Jehyeok Rew ),( Eenjun Hwang ) 한국인터넷정보학회 2020 KSII Transactions on Internet and Information Syst Vol.14 No.8

In recent years, as the environment has become an important issue in dealing with food, energy, and urban development, diverse environment-related applications such as environmental monitoring and ecosystem management have emerged. In such applications, automatic classification of animals using video or sound is very useful in terms of cost and convenience. So far, many works have been done for animal sounds classification using artificial intelligence techniques such as a convolutional neural network. However, most of them have dealt only with the sound of a specific class of animals such as bird sounds or insect sounds. Due to this, they are not suitable for classifying various types of animal sounds. In this paper, we propose a sound classification scheme based on a multi-feature network for classifying sounds of multiple species of animals. To do that, we first collected multiple animal sound datasets and grouped them into classes. Then, we extracted their audio features by generating mixed records and used those features for training. To evaluate the effectiveness of our scheme, we constructed an animal sound classification model and performed various experiments. We report some of the results.

On the validity of archeomagnetic dating method in Korea: a case study

Seungwon Shin,Yong-Hee Park,Daekyo Cheong,Sook Chung Shin 한국지질과학협의회 2018 Geosciences Journal Vol.22 No.1

An archeomagnetic study has been performed for six archeological relics of the Proto-Three Kingdoms period excavated in Jungdo Island, South Korea. Among a total of 90 samples from hearths remains of six dwelling sites, the characteristic remanent magnetization components were isolated from 70 samples using the progressive alternating field demagnetizations and considered to record faithfully a thermoremanent magnetization at the timing of the last cooling of the hearths. Two different approaches were made to determine the archeomagnetic ages: One is the conventional method using the relocated paleosecular variation (PSV) curve obtained from southwestern Japan. The other method adopts a Matlab archaeo_dating tool using a Bayesian statistics with global geomagnetic field prediction model (ARCH3K.1). Both archeomagnetic ages using two individual dating methods generally overlapped with the radiocarbon age ranges for each sites. However, no systematic relationship between archeomagnetic ages and radiocarbon ages was observed, implying that neither the relocated Japanese PSV data nor the global model accurately represent the Korean secular variation during the archeological period of this study. At present, archeomagnetic dating in Korea should be carefully applied in conjunction with other dating methods on the consideration of archeological context.

Enhancements in catalytic activity and duration of PdFe bimetallic catalysts and their use in direct formic acid fuel cells

Seungwon Yang,Yongjin Chung,이국승,Yongchai Kwon 한국공업화학회 2020 Journal of Industrial and Engineering Chemistry Vol.90 No.-

The palladium–iron based bimetallic catalysts (PdxFey/Cs) and facile synthetic method are introduced toenhance the catalytic activity and operational duration for direct formic acid fuel cells. To improve theproperties of PdxFey/C catalysts, such as degree of PdFe alloy and its crystallinity, heat treatment isconducted at 600 C. According to results, the Pd–Fe bond and Fe metal particle are formed after the heattreatment, while iron oxides and Pd particles are observed in the untreated samples. Electrochemicalevaluations for measuring the formic acid oxidation reaction rate demonstrates heat treated Pd3Fe1/C(HT-Pd3Fe1/C) is the best catalysts of six samples which are synthesized by using different Pd to Fe ratios(3:1, 1:1, 1:3) before and after heat treatment. This is because the HT-Pd3Fe1/C has high Pd–Fe alloyingand Pd contents. Through the heat treatment, the indirect formic acid oxidation reaction way is activatedand the resistance to CO poisoning is significantly improved. The maximum power density of directformic acid fuel cells using HT-Pd3Fe1/C whose open circuit voltage is 0.83 V is 137 mW cm 2, which is 1.6and 1.9 times higher than that of direct formic acid fuel cells using untreated catalyst and Pd/C.

The effect of intrinsic foot muscle training on medial longitudinal arch and ankle stability in patients with chronic ankle sprain accompanied by foot pronation

Chung, Kyoung A,Lee, Eunsang,Lee, Seungwon korean Academy of Physical Therapy Rehabilitation 2016 Physical therapy rehabilitation science Vol.5 No.2

Objective: The purpose of this study was to investigate whether the intrinsic foot muscle training method can improve the medial longitudinal arch in patients with chronic ankle injury and with pronated feet, as well as to investigate for the most effective exercise method for these patients. Design: Randomized controlled trial. Methods: Thirty men and women with pronated feet had participated in this study and were allocated to either the short foot exercise group (SFEG) or the towel curl exercise group (TCEG) randomly. SFEG and TCEG underwent exercises three times a week for 8 weeks, with three sets per day, totalling up to 5 minutes per day. The navicular drop test (NDT) was used in order to assess for changes in the medial longitudinal arch and the Cumberland ankle instability tool (CAIT) was used to assess for ankle instability of the chronic ankle sprain patients. Results: There was a significant increase in CAIT scores in the SFEG (p<0.05) and a significant difference between groups was presented (p<0.05). The NDT scores were significantly decreased in both groups (p<0.05). In the SFEG, the NDT scores were more decreased than in the TCEG (p<0.05). Conclusions: These results suggest that short foot exercises are more effective in providing intrinsic foot muscle training for patients with pronated feet among chronic ankle sprain patients. Furthermore, short foot exercises may be used to provide ankle stability.