http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Whole genome sequence of acute hepatopancreatic necrosis (AHPND) isolated from Korea
Seong Don Hwang,Jee Youn Hwang,Byeong Chul Kang,Hae Ryeon Jeon,Won Joo Chun,Da won Lee,Sae bom Sohn,Su Mi Kim,Seok Ryel Kim,Kyoo Yeol Lee,Myung Hee Jung,Jung Soo Seo,Mun-Gyeong Kwon,Bo Young Jee 한국수산해양기술학회(구 한국어업기술학회) 2018 한국수산해양기술학회 학술발표대회 Vol.2018 No.11
Hwang, Seong Don,Ohtani, Maki,Hikima, Jun-ichi,Jung, Tae Sung,Kondo, Hidehiro,Hirono, Ikuo,Aoki, Takashi Elsevier 2012 DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY Vol.37 No.1
<P><B>Highlights</B></P><P>► We cloned the Japanese flounder TLR3 (JfTLR3) gene. ► Gene expression of JfTLR3 was up-regulated in PBLs after stimulation with poly I:C and CpG ODN 1668. ► JfTLR3 gene was induced in different tissues following infection with VHSV. ► Intracellular poly I:C in JfTLR3-overexpressing YO-K cells activates IFN and NF-κB pathways. ► In JfTLR3-overexpressing HINAE cells, extracellular poly I:C activates IFN and NF-κB pathways.</P> <P><B>Abstract</B></P><P>Mammalian Toll-like receptor 3 (TLR3) recognizes extracellular and intracellular viral dsRNA, and then initiates signaling cascades leading to NF-κB activation and interferon (IFN) production. To understand the roles of TLR3 in the fish immune system, TLR3 gene (JfTLR3) was identified from Japanese flounder (<I>Paralichthys olivaceus</I>), which consisted of 4 exons and 3 introns. Its expression in peripheral blood leukocytes increased upon stimulation with poly I:C and CpG ODN 1668. Exposure to viral hemorrhagic septicemia virus increased expression of JfTLR3 in the blood, liver, head kidney and spleen. Intracellular poly I:C stimulation in JfTLR3-overexpressing YO-K cells significantly induced IFN-inducible and NF-κB-regulated genes. NF-κB activity in JfTLR3-overexpressing YO-K cells was significantly induced by intracellular poly I:C while expression of IFN-inducible genes and NF-κB reporter activity in JfTLR3-overexpressing HINAE cells increased upon stimulation by extracellular poly I:C. These results suggest that JfTLR3 plays an important role in the induction of antiviral immune response.</P>
신속한 항생제 감수성 테스트 법의 개발을 위한 Water-Soluble Tetrazolium Salt의 적용
황성돈 ( Seong Don Hwang ),조동희 ( Dong Hee Jo ),김광일,조미영 ( Mi Young Cho ),지보영 ( Bo Young Jee ),박명애 ( Myoung Ae Park ),박찬일 ( Chan Il Park ) 한국어병학회 2015 한국어병학회지 Vol.28 No.2
In this study, we conducted to the development of a rapid antimicrobial susceptibility test method using WST-1 which is known to water-soluble tetrazolium salt, in order to rapidly response against bacterial diseases in fish. Eight of antibiotics which are permissioned for marine organism from government were used to rapid antimicrobial susceptibility testing using the WST-1. As a result, a similar tendency was verified compare to conventional antibiotic susceptibility test results. Generally, the antibiotic susceptibility test method required about 3 days (72 hours) for determine the effective antibiotics, however, we have confirmed that the our method using WST-1 was required at least 36 hours in this study. Consequentially, our method will contribute to development of rapid antimicrobial susceptibility testing for bacterial diseases in fish.
The rice RING E3 ligase OsHIR1 participates in positive regulation of arsenic and cadmium uptake
Sung Don Lim,Jin Gyu Hwang,Cheol Seong Jang 한국육종학회 2013 한국육종학회 심포지엄 Vol.2013 No.07
The metalloid arsenic (As) and the hevy metal cadmium (Cd) are ubiquitously found at low concentrations in the earth, while high concentrations of the both elements in soil and crop are severe dangerous to human health. We have tried to retrieve RING E3 ligase gene, which is believed to regulate substrate proteins in As or Cd uptake via ubiquitin 26S proteasome pathway, related to inhibit metal ion transport system. A total of 48 rice RING E3 ligases were randomly selected and then conducted semi-quantitative RT-PCR for their expression patterns as exposed to As and Cd treatments. We discovered one gene, Oryza sativa heavy metal induced RING E3 ligase 1 (OsHIR1) that was significantly up-regulated against both treatments. A total of 31 positive interaction clones with OsHIR1 were screened depending on their strong α-galactosidase activity via yeast-two hybrid screen. Bimolecular fluorescence complementation analysis evidenced that the OsHIR1 protein was clearly interacted with each of six partner protein including aquaporin tonoplast intrinsic protein 4;1 (OsTIP4;1) in the plasma membrane. Protein degradation assay showed that OsHIR1 strongly degraded the protein level of OsTIP4;1 via ubiquitin 26S proteasome system. Heterogeneous overexpression of OsHIR1 in Arabidopsis showed As- and Cd-insensitive phenotype. In addition, the transgenic plant showed low levels of As and Cd accumulation than the control plant in leaf and root. Here, we report the novel finding that OsHIR1 E3 ligase positively regulates OsTIP4;1 related to As and Cd uptake.