Microbial Transformation of Ginseng Saponin by Lactobacillus pentosus 6105

Se-Hwa Kim(김세화),Ho-Bin Kim,Jin-Woo Min,Lin-Hu Quan,Deok-Chun Yang 고려인삼학회 2008 고려인삼학회 학술대회 Vol.2008 No.12

Transformation of Ginsenoside Rd into F2 and Compound K by Lactobacillus pentosus LH6 Isolated from Kimchi

Lin-Hu Quan(전림호),Ho-Bin Kim,Se-Hwa Kim,Yeon-Ju Kim,Deok Chun Yang 고려인삼학회 2008 고려인삼학회 학술대회 Vol.2008 No.12

Bioconversion of Ginsenoside Rd into Compound K by Lactobacillus pentosus DC101 Isolated from Kimchi

Lin-Hu Quan,Le-Qin Cheng,Ho-Bin Kim,Ju-Han Kim,Na-Ri Son,Se-Young Kim,Hyun-O Jin,Deok-Chun Yang 고려인삼학회 2010 Journal of Ginseng Research Vol.34 No.4

Ginsenosides are the principal components responsible for the pharmacological and biological activities of ginseng. Ginsenoside Rd was transformed into compound K using cell-free extracts of food microorganisms, with Lactobacillus pentosus DC101 isolated from kimchi (traditional Korean fermented food) used for this conversion. The optimum time for the conversion was about 72 h at a constant pH of 7.0 and an optimum temperature of about 30°C. The transformation products were identified by thin-layer chromatography and high-performance liquid chromatography, and their structures were assigned using nuclear magnetic resonance analysis. Generally, ginsenoside Rd was converted into ginsenoside F2 by 36 h post-reaction. Consequently, over 97% of ginsenoside Rd was decomposed and converted into compound K by 72 h post-reaction. The bioconversion pathway to produce compound K is as follows: ginsenoside Rd→ginsenoside F2→compound K.

Stenotrophomonas ginsengisoli sp. nov., isolated from a ginseng field

Kim, Ho-Bin,Srinivasan, Sathiyaraj,Sathiyaraj, Gayathri,Quan, Lin-Hu,Kim, Se-Hwa,Bui, Thi Phuong Nam,Liang, Zhi-qi,Kim, Yeon-Ju,Yang, Deok-Chun Microbiology Society 2010 International journal of systematic and evolutiona Vol.60 No.7

<P>A Gram-negative, non-spore-forming, rod-shaped bacterium, designated strain DCY01<SUP>T</SUP>, was isolated from soil from a ginseng field in South Korea and was characterized in order to determine its taxonomic position. 16S rRNA gene sequence analysis revealed that strain DCY01<SUP>T</SUP> belonged to the <I>Gammaproteobacteria</I> and was most closely related to <I>Stenotrophomonas koreensis</I> KCTC 12211<SUP>T</SUP> (98.4 % similarity), <I>Stenotrophomonas humi</I> R-32729<SUP>T</SUP> (97.2 %), <I>Stenotrophomonas terrae</I> R-32768 (97.1 %), <I>Stenotrophomonas maltophilia</I> DSM 50170<SUP>T</SUP> (96.9 %) and <I>Stenotrophomonas nitritireducens</I> DSM 12575<SUP>T</SUP> (96.8 %). Chemotaxonomic analyses revealed that strain DCY01<SUP>T</SUP> possessed a quinone system with Q-8 as the predominant compound, and iso-C15 : 0 (28.2 %), C16 : 0 10<I>-</I>methyl (13.2 %), iso-C15 : 1 F (10.8 %) and C15 : 0 (7.5 %) as major fatty acids, corroborating assignment of strain DCY01<SUP>T</SUP> to the genus <I>Stenotrophomonas</I>. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The results of DNA-DNA hybridization and physiological and biochemical tests clearly demonstrated that strain DCY01<SUP>T</SUP> represents a species distinct from recognized <I>Stenotrophomonas</I> species. Based on these data, DCY01<SUP>T</SUP> (=KCTC 12539<SUP>T</SUP>=NBRC 101154<SUP>T</SUP>) should be classified as the type strain of a novel species of the genus <I>Stenotrophomonas</I>, for which the name <I>Stenotrophomonas ginsengisoli</I> sp. nov. is proposed.</P>

Iron Homeostasis and Energy Metabolism in Obesity

( Se Lin Kim ),( Sunhye Shin ),( Soo Jin Yang ) 한국임상영양학회 2022 Clinical Nutrition Research Vol.11 No.4

Iron plays a role in energy metabolism as a component of vital enzymes and electron transport chains (ETCs) for adenosine triphosphate (ATP) synthesis. The tricarboxylic acid (TCA) cycle and oxidative phosphorylation are crucial in generating ATP in mitochondria. At the mitochondria matrix, heme and iron-sulfur clusters are synthesized. Iron-sulfur cluster is a part of the aconitase in the TCA cycle and a functional or structural component of electron transfer proteins. Heme is the prosthetic group for cytochrome c, a principal component of the respiratory ETC. Regarding fat metabolism, iron regulates mitochondrial fat oxidation and affects the thermogenesis of brown adipose tissue (BAT). Thermogenesis is a process that increases energy expenditure, and BAT is a tissue that generates heat via mitochondrial fuel oxidation. Iron deficiency may impair mitochondrial fuel oxidation by inhibiting iron-containing molecules, leading to decreased energy expenditure. Although it is expected that impaired mitochondrial fuel oxidation may be restored by iron supplementation, its underlying mechanisms have not been clearly identified. Therefore, this review summarizes the current evidence on how iron regulates energy metabolism considering the TCA cycle, oxidative phosphorylation, and thermogenesis. Additionally, we relate iron-mediated metabolic regulation to obesity and obesity-related complications.

AMESim software를 이용한 엔진 폐열 회수율 조사 및 최적 시스템 개발

김세린(Se-lin Kim),최경욱(Kyung-wook Choi),이기형(Ki-hyung Lee),김기범(Ki-bum Kim) 대한기계학회 2014 대한기계학회 춘추학술대회 Vol.2014 No.11

In recent, automobile engineers have paid much attention on the waste heat recovery technologies, one of possible means to improve thermal efficiency of automotive engine. Two superheaters for the heat recovery in V-type gasoline engine is not cost effective. Therefore, finding the optimum position for one superheater is always tricky decision. The superheaterd better be installed as near exhaust manifold as possible for higher effectiveness; however, only half of exhaust mass flow could be utilized for heat recovery. With the fact in mind, the optimum position of one superheater was investigated with simulation models developed using a commercial software (i.e. AMESim). It was found that installing the superheater near the exhaust manifold regardless of utilizing only half of exhaust mass flow, could recover 3.8 kW more energy from the engine exhaust. Based on this result, the optimum layout of an automotive waste heat recovery system was developed and proposed in this paper.

자동차 배기폐열 회수용 Rankine Cycle System의 Superheater 부착 위치에 따른 폐열회수율 조사

김세린(Se-Lin Kim),최경욱(Kyng-Wook Choi),이기형(Ki-Hyung Lee),김기범(Ki-Bum Kim) 한국산학기술학회 2014 한국산학기술학회 학술대회 Vol.- No.-

최근 환경문제에 대한 규제가 심해지면서 이를 해결하기 위해 버려지는 열을 회수하여 다시 사용하는 폐열 회수 시스템에 관한 연구가 활발하게 진행되고 있다. 본 논문에서는 1-D simulation 프로그램인 AMESim software를 이용하여 대표적인 폐열회수 시스템인 Rankine-Cycle System을 Modeling하고, 자동차의 배기가스 관을 따라 부착되는 Superheater와 Boiler의 위치에 따라 폐열 회수 열량이 얼마나 되는지 조사하였다. 연구 결과, 두 개의 배기다기관이 합류하는 지점에 Superheater를 부착하는 경우(Model B)보다 엔진과의 거리가 가까운 하나의 배기다기관에 부착하여(Model A) 폐열 회수시 약 2.4kW의 회수 열량을 더 얻을 수 있다는 것을 확인하였다.

Autoclave와 Hot pressing 공법으로 제작된 탄소섬유복합소재 블럭의 온도 분포 해석

김세린(Se-Lin Kim),서형석(Hyoung-Seok Seo),김기범(Ki-Bum Kim) 한국산학기술학회 2014 한국산학기술학회 학술대회 Vol.- No.-

최근 우주 왕복선에 사용된 복합소재인 탄소섬유강화플라스틱(Carbon Fiber Reinforced Plastics)은 가볍고, 단단하며 열 하중에 잘 견디는 이점 때문에 다른 분야에서도 큰 인기를 얻고 있다. 탄소 섬유 블록은 Hot Pressing이나 Autoclave 등의 경화 공법으로 제작되는데 제작 과정 중 경화정도가 일정하 지 않기 때문에 블록을 제작하는데 있어 심각한 결함을 야기하기도 한다. 따라서 탄소 섬유 블록의 경 화 과정에서 전도, 대류, 복사를 포함한 열전달 현상은 반드시 이해되어야 하며 본 논문은 에폭시로 접착하여 적층한 탄소 섬유 블록 경화 과정에서의 열전달 현상과 온도 분포 해석에 목적을 두고 있다.

도시 오픈스페이스에서의 콘텍스트적 접근을 통한 경관조명디자인 표현 연구

김세린 ( Kim Se Lin ),김연정 ( Kim Yeon Jung ) 한국기초조형학회 2018 기초조형학연구 Vol.19 No.1

현대 도시디자인의 패러다임은 사람과 사람들이 활동하는 장소 중심의 수평적 네트워크를 중요한 가치로 두며, 도시환경에서 보다 친밀하고 사회ㆍ문화적으로 풍부한 공공공간을 요구한다. 또한 야간 활동 시간대의 증가는 다양하게 즐길 수 있는 야간 환경을 필요로 하지만 현재의 야간 환경은 공간적 가치와 유용성에 한계가 있다. 이에 따라 본 연구는 장소와 이용자 중심적 이해를 바탕으로 하는 콘텍스트적 접근을 통해 도시 오픈스페이스에서의 경관조명디자인 방향성을 제시하는데 목적이 있다. 우선 관련문헌 및 선행연구를 통해 도시 오픈스페이스, 경관조명디자인, 콘텍스트의 개념 및 콘텍스트적 접근의 의의에 대한 이론적 고찰을 하여 도시 오픈스페이스 경관조명디자인에서 콘텍스트적 접근의 필요성을 밝혔다. 다음으로 콘텍스트 유형과 경관조명 역할을 연계하여 경관조명디자인의 콘텍스트적 접근 구조를 제시하고, 이를 토대로 상징적 개념, 시각적 심미성, 행태적 상호작용성 측면에서 사례 분석을 진행하여 콘텍스트적 접근을 통한 경관조명디자인의 표현 및 효과를 도출하였다. 분석 결과는 다음과 같다. 1)상징적 콘텍스트의 정체성 표현은 빛을 통한 직접적 오브제화, 은유적 형상화, 형태ㆍ구조의 입체화로 나타나며, 이러한 표현은 장소정체성을 뚜렷하게 함으로써 장소 가치에 대한 정서적 유대감을 형성했다. 2)시각적 콘텍스트의 심미성 표현은 수평적ㆍ수직적 밝기 위계, 다양한 빛 볼륨의 리듬과 대비, 건축화 조명 디테일로 나타나며, 이는 전반적으로 조화로우며 입체적인 공간성을 창출했다. 3)행태적 콘텍스트의 상호작용적 표현은 비일상적 빛, 장면적ㆍ연속적 경관 구성, 신기술ㆍ매체와의 결합으로 다양한 행태를 지원하며 공간경험을 풍부하게 했다. 이러한 콘텍스트적 접근에 의한 경관조명디자인이 도시 환경에서의 사람과 환경간의 상호 관계성을 증진하며 장소성을 강화할 수 있음을 밝힌 데에 연구의 의의가 있다. The paradigm of contemporary urban design sets the horizontal network of people and place as an important value and it demands the urban environment to have more intimate public spaces that are socially and culturally rich. In addition, the increase of the nighttime activities requires enjoyable environment that can afford various nighttime activities, but the current condition is limited in such spatial value and usability. Therefore, this study aims to propose the direction for outdoor lighting design in urban open spaces through a contextual approach based on the place and user-centered understanding. First, theoretical study about urban open spaces; outdoor lighting; context and contextual approach was conducted. The significance of the contextual approach in outdoor lighting design was revealed through literature and advanced research reviews. Next, the structure of contextual approach was suggested and based on it, case study was conducted in terms of symbolic concept, visual aesthetics and behavioral interactions, which have drawn the expression and the effects of outdoor lighting design. 1) The expression of the identity from symbolic context appears as: direct objectification, metaphoric representation, and modeling of form and structure by light. These expressions formed emotional connectedness with the place values by clarifying place identities. 2) The aesthetic expressions of the visual context appears as: horizontal and vertical hierarchy of brightness, the rhythm and contrast formed by diverse light volumes, and the light integrated with architecture. It created an overall harmonious and three- dimensional space. 3) Interactive expressions of behavioral appears as: the use of nonusual lighting, scenic and sequential nightscape, and the combination of new technological media and light. It afforded various behaviors and enriched the spatial experience. The significance of this study is that it suggests one of the ways to enhance the interrelation between people and the environment, and strengthen the placeness through outdoor lighting design.

설계 초기단계에서 다변량 통계 모델링 기반 건물에너지 평가

김영록(Kim, Young-Rok),김종희(Kim, Jong-Hee),오세민(Oh, Se-Min),김종헌(Kim, Jong-Hun),박상린(Park, Sang-Lin) 대한건축학회 2013 대한건축학회 학술발표대회 논문집 Vol.33 No.2