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Manoj Kumar Reddy Somagutta,Maria Kezia Lourdes Pormento,Muhammad Adnan Khan,Alaa Hamdan,Namrata Hange,Manish KC,Sukrut Pagad,Molly Sanjay Jain,Sivasthikka Lingarajah,Vishal Sharma,Jaspreet Kaur,Berna 대한중환자의학회 2021 Acute and Critical Care Vol.36 No.3
Background: Previous studies have suggested favorable outcomes of hydrocortisone, ascorbic acid (vitamin C), and thiamine (HAT) therapy in patients with sepsis. However, similar results have not been duplicated in sequential studies. This meta-analysis aimed to reevaluate the value of HAT treatment in patients with sepsis. Methods: Electronic databases were searched up until October 2020 for any studies that compared the effect of HAT versus non-HAT use in patients with sepsis. Results: Data from 15 studies (eight randomized controlled trials [RCTs] and seven cohort studies) involving 67,349 patients were included. The results from the RCTs show no significant benefit of triple therapy on hospital mortality (risk ratio [RR], 0.99; P=0.92; I2=0%); intensive care unit (ICU) mortality (RR, 0.77; P=0.20; I2=58%); ICU length of stay (weighted mean difference [WMD], 0.11; P=0.86; I2=37%) or hospital length of stay (WMD: 0.57; P=0.49; I2=17%), and renal replacement therapy (RR, 0.64; P=0.44; I2=39%). The delta Sequential Organ Failure Assessment (SOFA) score favored treatment after a sensitivity analysis (WMD, –0.72; P=0.01; I2=32%). However, a significant effect was noted for the duration of vasopressor use (WMD, –25.49; P<0.001; I2=46%). The results from cohort studies have also shown no significant benefit of HAT therapy on hospital mortality, ICU mortality, ICU length of stay, length of hospital stay, the delta SOFA score, the use of renal replacement therapy, or vasopressor duration. Conclusions: HAT therapy significantly reduced the duration of vasopressor use and improved the SOFA score but appeared not to have significant benefits in other outcomes for patients with sepsis. Further RCTs can help understand its benefit exclusively.
Effect of Sulfamerazine on Structural Characteristics of Sodium Alginate Biopolymeric Films
Yerramathi Babu Bhagath,이소연,Manjula Kola,TATA SANJAY KANNA SHARMA,Annem Muniraj Beulah,Y. Veera Manohara Reddy,박태정,박종필,Ravi Sahukari,G. Madhavi 한국생물공학회 2022 Biotechnology and Bioprocess Engineering Vol.27 No.4
The present study was intended to describe and understand the mechanical, structural, morphological, and antimicrobial properties of sodium alginate (SA) biopolymeric films doped with sulfamerazine (SMR). Tensile strength (TS), elongation at break (E%), intermolecular interactions, crystallinity, structural integrity, and surface properties were investigated for SMR concentrations of 0.6, 1.2, and 1.8 mg/mL in the film-forming solution. The TS and E% of the various experimental films differed significantly according to the SMR concentration (p < 0.05). However, a slight reduction was observed at the highest SMR concentration (1.8 mg/mL). Fourier transform infrared spectroscopy and X-ray diffraction patterns revealed the reaction of the functional groups and conformational changes caused by the SMR and Ca2+ ions in the film matrix. Scanning electron microscopy and atomic force microscopy image analysis illustrated the cross-sectional microstructure and surface roughness of the experimental films. Overall, the doping of SMR into the SA polymer matrix was proven to have a significant effect on the morphological, structural, and mechanical properties of the resulting films.
Isolation of subgroup J avian leukosis virus in Korea
Haan-Woo Sung,Jae-Hong Kim,Sanjay Reddy,Aly Fadly 대한수의학회 2002 Journal of Veterinary Science Vol.3 No.2
Two subgroup J avian leukosis viurses (ALVs) wereisolated from broiler breeder flocks,in which myeloidleukosis had occurred.The isolates could be classifiedas subgroup JALV by the positive reaction in polymerasechain reaction (PCR) with primers specific for subgroupJ ALV. Two isolates replicated in chicken embryofibroblast (CEF) cells from the alv6 chicken line inwhich cells are resistant to subgroup A and E ALVs.In in vitro serum neutralization tests with othersubgroup ALVs including ADOL-Hc1,the prototype ofsubgroup J ALVs isolated in the United States ofAmerica, two isolates were partially neutralized byantibody to ADOL-Hc1, indicating that Korean isolatesand ADOL-Hc1 may be antigenically related, but notidentical.When the PCR was done with a primer pairdesigned to amplify genes of E element and longterminal repeat of proviral DNA, the PCR productsize of one isolate (KOAL-PET) was smaller than thatof ADOL-Hc1, suggesting that some sequences inthese regions are deleted.
Inhibition of Microbial Quorum Sensing Mediated Virulence Factors by Pestalotiopsis sydowiana
Paramanantham Parasuraman,B Devadatha,V. Venkateswara Sarma,Sampathkumar Ranganathan,Dinakara Rao Ampasala,Dhanasekhar Reddy,Ranjith Kumavath,김인원,Sanjay K. S. Patel,Vipin Chandra Kalia,이정걸,Busi Siddha 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.4
Quorum sensing (QS)-mediated infections cause severe diseases in human beings. The control of infectious diseases by inhibiting QS using antipathogenic drugs is a promising approach as antibiotics are proving inefficient in treating these diseases. Marine fungal (Pestalotiopsis sydowiana PPR) extract was found to possess effective antipathogenic characteristics. The minimum inhibitory concentration (MIC) of the fungal extract against test pathogen Pseudomonas aeruginosa PAO1 was 1,000 μg/ml. Sub-MIC concentrations (250 and 500 μg/ml) of fungal extract reduced QSregulated virulence phenotypes such as the production of pyocyanin, chitinase, protease, elastase, and staphylolytic activity in P. aeruginosa PAO1 by 84.15%, 73.15%, 67.37%, 62.37%, and 33.65%, respectively. Moreover, it also reduced the production of exopolysaccharides (74.99%), rhamnolipids (68.01%), and alginate (54.98%), and inhibited the biofilm formation of the bacteria by 90.54%. In silico analysis revealed that the metabolite of P. sydowiana PPR binds to the bacterial QS receptor proteins (LasR and RhlR) similar to their respective natural signaling molecules. Cyclo(- Leu-Pro) (CLP) and 4-Hydroxyphenylacetamide (4-HPA) were identified as potent bioactive compounds among the metabolites of P. sydowiana PPR using in silico approaches. The MIC values of CLP and 4-HPA against P. aeruginosa PAO1 were determined as 250 and 125 μg/ml, respectively. All the antivirulence assays were conducted at sub-MIC concentrations of CLP (125 μg/ml) and 4-HPA (62.5 μg/ml), which resulted in marked reduction in all the investigated virulence factors. This was further supported by gene expression studies. The findings suggest that the metabolites of P. sydowiana PPR can be employed as promising QS inhibitors that target pathogenic bacteria.