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Waterborne Pathogens Identification in Public Bathroom by PCR-Reverse Blot Hybridization Assay
Choi, Seung-Gu,Song, Woon-Heung,Lee, Jae-Sang,Yang, Byoung-Seon,Choi, Myeong-Sik 대한임상검사과학회 2011 대한임상검사과학회지(KJCLS) Vol.43 No.3
A total of 30 water samples were collected from 30 different public baths in Seoul, Korea. Contamination of public bath water by waterborne pathogens can cause disease outbreaks and contribute to increase background rates of disease. Pathogens in water was filtered by nitrocellulose membrane with $0.45{\mu}m$ pore size. The membrane filters were analyzed by both cultivation and polymerase chain reaction (PCR) amplification of partial 16S rRNA gene. Various microorganisms including 4 Escherichia coli/Shigella spp. 1 Salmonella spp. 3 Pseudomonas aeruginosa and 2 Mycobacterium spp. were identified by reverse blot hybridization assay (REBA). PCR-REBA was able to identify many bacterial genera in one assay. Our results suggest that appropriate hygiene practice and continuous monitoring is needed for reducing health risk associated with public bath houses.
Choi, Dae Woon,Kwon, Da-Ae,Jung, Sung Keun,See, Hye-Jeong,Jung, Sun Young,Shon, Dong-Hwa,Shin, Hee Soon Informa UK (TaylorFrancis) 2018 Bioscience, Biotechnology, and Biochemistry Vol.82 No.9
<P>Allergic contact dermatitis (ACD) is an inflammatory skin disease caused by hapten-specific immune response. Silkworm droppings are known to exert beneficial effects during the treatment of inflammatory diseases. Here, we studied whether topical treatment and oral administration of silkworm dropping extract (SDE) ameliorate trimellitic anhydride (TMA)-induced ACD. In ACD mice model, SDE treatment significantly suppressed the increase in both ear thickness and serum IgE levels. Furthermore, IL-1 and TNF- levels were reduced by SDE. In allergic responses, SDE treatment significantly attenuated the production of the Th2-associated cytokine IL-4 in both ear tissue and draining lymph nodes. However, it increased the production of the Th1-mediated cytokine IL-12. Thus, these results showed that SDE attenuated TMA-induced ACD symptoms through regulation of Th1/Th2 immune response. Taken together, we suggest that SDE treatment might be a potential agent in the prevention or therapy of Th2-mediated inflammatory skin diseases such as ACD and atopic dermatitis.Abbreviations: ACD: allergic contact dermatitis; AD: atopic dermatitis; APC: antigen presenting cells; CCL: chemokine (C-C motif) ligand; CCR: C-C chemokine receptor; Dex: dexamethasone; ELISA: enzyme-linked immunosorbent assay; IFN: interferon; Ig: immunoglobulin; IL: interleukin; OVA: ovalbumin; PS: prednisolone; SDE: silkworm dropping extract; Th: T helper; TMA: trimellitic anhydride; TNF: tumor necrosis factor</P>
GaN light-emitting diodes on glass substrates with enhanced electroluminescence
Choi, Jun Hee,Ahn, Ho Young,Lee, Yun Sung,Park, Kyungwoo,Kim, Tae-Ho,Cho, Kyung Sang,Baik, Chan Wook,Kim, Sun Il,Yoo, Hyobin,Lee, Eun Hong,Choi, Byoung Lyong,Kim, Sung-Dae,Kim, Young-Woon,Kim, Miyoung The Royal Society of Chemistry 2012 Journal of materials chemistry Vol.22 No.43
<P>We report the enhanced electroluminescence (EL) of GaN light-emitting diodes (LEDs) on glass substrates by controlling GaN crystal morphology, crystallinity, and device fabrication. Depending on the degree of epitaxy, we studied three different GaN morphologies; randomly oriented GaN polycrystals, nearly single-crystalline pyramid arrays, and fully single-crystalline pyramid arrays, which were fabricated by controlling the epitaxial relationship with the substrates. At proper growth temperature, GaN crystallinity was improved with increasing GaN crystal size irrespective of the GaN crystallographic orientation, as determined by spatially resolved cathodoluminescent spectroscopy. All the different GaN morphologies were further fabricated into LEDs to investigate their EL characteristics. The optimized GaN LEDs on glass composed of the nearly single-crystalline GaN pyramid arrays exhibited excellent microscopic EL uniformity and luminance values of about 2700 and 1150 cd m<SUP>−2</SUP> at peak wavelengths of 537 and 480 nm, respectively.</P> <P>Graphic Abstract</P><P>GaN LEDs on glass exhibiting excellent microscopic and macroscopic uniformities by optimizing GaN pyramid crystallinity and related process. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c2jm34405j'> </P>
Sung Ho Lee,Tae-Hoon Kim,Yong-Seog Oh,Seil Oh,Jong-Il Choi,Jin-Bae Kim,Jong-Chun Nah,Sung Il Im,Ki-Woon Kang,Seongwook Han,June Soo Kim 대한의학회 2020 Journal of Korean medical science Vol.35 No.2
Background: An implantable loop recorder (ILR) is an effective tool for diagnosing unexplained syncope (US). We examined the diagnostic utility of an ILR in detecting arrhythmic causes of US and determining which clinical factors are associated with pacemaker (PM) implantation. Methods: This retrospective, multicenter, observational study was conducted from February 2006 to April 2018 at 11 hospitals in Korea. Eligible patients with recurrent US received an ILR to diagnose recurrent syncope and document arrhythmia. Results: A total of 173 US patients (mean age, 67.6 ± 16.5 years; 107 men [61.8%]) who received an ILR after a negative conventional workup were enrolled. During a mean follow-up of 9.4 ± 11.1 months, 52 patients (30.1%) had recurrent syncope, and syncope- correlated arrhythmia was confirmed in 34 patients (19.7%). The ILR analysis showed sinus node dysfunction in 24 patients (70.6%), supraventricular tachyarrhythmia in 4 (11.8%), ventricular arrhythmia in 4 (11.8%), and sudden atrioventricular block in 2 (5.9%). Overall, ILR detected significant arrhythmia in 99 patients (57.2%) irrespective of syncope. Among patients with clinically relevant arrhythmia detected by ILR, PM implantation was performed in 60 (34.7%), an intra-cardiac defibrillator in 5 (2.9%), and catheter ablation in 4 (2.3%). In a Cox regression analysis, history of paroxysmal atrial fibrillation (PAF) (hazard ratio [HR], 2.34; 95% confidence interval [CI], 1.33–4.12; P < 0.01) and any bundle branch block (BBB) (HR, 2.52; 95% CI, 1.09–5.85; P = 0.03) were significantly associated with PM implantation. Conclusion: ILR is useful for detecting syncope-correlated arrhythmia in patients with US. The risk of PM is high in US patients with a history of PAF and any BBB.
Choi, Sun-Mi,Choi, Ji-Hun,Choi, Yun-Sang,Kim, Hyun-Wook,Kim, Hack-Youn,Lee, Mi-Ai,Hwang, Ko-Eun,Lee, Ju-Woon,Kim, Cheon-Jei Korean Society for Food Science of Animal Resource 2011 한국축산식품학회지 Vol.31 No.5
In this study, the effects of kimchi powder on the quality properties of an emulsion type sausage made with irradiated pork meat were investigated. Raw ground pork ham was gamma-irradiated at 5 and 10 kGy, and the emulsion type sausage was then manufactured with the irradiated pork meat. The pH value was shown to increase by irradiation of raw material (p<0.05). Samples containing kimchi powder had a lower pH value than other treatments, but no significant differences were observed. In a sensory evaluation, the scores of all the criteria were decreased with an increased irradiation dose of raw material, but samples containing kimchi powder had a higher sensory score than other treatments in all irradiation doses of raw material. The TBARS values were increased by irradiation of raw material and the samples containing kimchi powder had the highest value (p<0.05). Odor substances analyzed by an electronic nose were increased with increased irradiation dose of raw material in all treatments, and samples containing kimchi powder showed a higher increase of odor substances than other treatments. Thus, this study demonstrated that kimchi powder was effective in eliminating the off-odor and flavor of sausages made with irradiated pork meat despite the increase of the TBARS value.
One-step graphene coating of heteroepitaxial GaN films
Choi, Jae-Kyung,Huh, Jae-Hoon,Kim, Sung-Dae,Moon, Daeyoung,Yoon, Duhee,Joo, Kisu,Kwak, Jinsung,Chu, Jae Hwan,Kim, Sung Youb,Park, Kibog,Kim, Young-Woon,Yoon, Euijoon,Cheong, Hyeonsik,Kwon, Soon-Yong IOP Pub 2012 Nanotechnology Vol.23 No.43
<P>Today, state-of-the-art III-Ns technology has been focused on the growth of c-plane nitrides by metal–organic chemical vapor deposition (MOCVD) using a conventional two-step growth process. Here we show that the use of graphene as a coating layer allows the one-step growth of heteroepitaxial GaN films on sapphire in a MOCVD reactor, simplifying the GaN growth process. It is found that the graphene coating improves the wetting between GaN and sapphire, and, with as little as ∼0.6 nm of graphene coating, the overgrown GaN layer on sapphire becomes continuous and flat. With increasing thickness of the graphene coating, the structural and optical properties of one-step grown GaN films gradually transition towards those of GaN films grown by a conventional two-step growth method. The InGaN/GaN multiple quantum well structure grown on a GaN/graphene/sapphire heterosystem shows a high internal quantum efficiency, allowing the use of one-step grown GaN films as ‘pseudo-substrates’ in optoelectronic devices. The introduction of graphene as a coating layer provides an atomic playground for metal adatoms and simplifies the III-Ns growth process, making it potentially very useful as a means to grow other heteroepitaxial films on arbitrary substrates with lattice and thermal mismatch. </P>