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Characterization of Methionine Analogue Resistant Mutant Cephalosporium acremonium MAR-80
Lee, Jung Joon,Mheen, Tae Ick,Choi, Sang Ho,Lee, Kyoung 한국균학회 1987 韓國菌學會誌 Vol.15 No.2
Cephalosporium acremonium MAR-80, a strain of methionine analogue resistant mutant, showed good activity of sulfate utilization as only sulfur source. The effect of methionine on the sulfate uptake system was investigated by using Na₂^(35) SO₄ as a tracer in the resting cell system. And then, it was revealed that sulfate permease of this strain was less repressed and/or less inhibited by methionine than parent type. This deregulation was due to low activity of methionine uptake, which was operated by diffusion to a certain extent. From these studies, it could be anticipated that the improved productivity of Cephalosporin C and lower dependent of Cephalosporin C production on methionine were related to the increased uptake rate of sulfate.
Lee, Sang Ick,Park, Se Yeoun,Chung, Young Keun WILEY-VCH Verlag 2006 Advanced synthesis & catalysis Vol.348 No.16
<P>A new [Rh(cod)Cl]<SUB>2</SUB>/AgX (cod=1,5-cyclooctadiene)-catalyzed intermolecular [4+2+2] cycloaddition reaction between two enynes with one diene, and between two diynes and one diene has been developed. This is the first example of a rhodium-catalyzed trimolecular and three-component [4+2+2] cycloaddition to form eight-membered ring compounds. The yield of the reaction was highly dependent upon the counteranion and the reaction medium. The best yields were obtained when the reaction was carried out in toluene with OTf<SUP>−</SUP> anions. The regioselectivity was highly dependent upon the substrates. Relatively high regioselectivities were obtained for the cycloaddition of two enynes with one diene. In some cases, only one regioisomer was obtained. However, the regioselectivities for the cycloaddition of two diynes with one diene were rather poor. The use of a syringe pump in the slow addition of reactants slightly enhanced the regioselectivity.</P> <B>Graphic Abstract</B> <P> <img src='wiley_img/16154150-2006-348-16-17-ADSC200600321-content.gif' alt='wiley_img/16154150-2006-348-16-17-ADSC200600321-content'> </P>
Rhodium N-Heterocyclic Carbene Catalyzed [4 + 2] and [5 + 2] Cycloaddition Reactions.
Lee, Sang Ick,Park, Se Yeoun,Park, Ji Hoon,Jung, Il Gu,Choi, Soo Young,Chung, Young Keun,Lee, Bun Yeoul WILEY-VCH Verlag 2006 Chem Inform Vol.37 No.21
<P>ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.</P>
Silicone-Based Adhesives with Highly Tunable Adhesion Force for Skin-Contact Applications
Lee, Bong Kuk,Ryu, Jin Hwa,Baek, In-Bok,Kim, Yarkyeon,Jang, Won Ick,Kim, Sang-Hyeob,Yoon, Yong Sun,Kim, Seung Hwan,Hong, Seong-Gu,Byun, Sangwon,Yu, Han Young Wiley (John WileySons) 2017 Advanced healthcare materials Vol.6 No.22
<i>TP53</i> Mutations in Korean Patients with Non-small Cell Lung Cancer
Lee, Eung Bae,Jin, Guang,Lee, Shin Yup,Park, Ji Young,Kim, Min Jung,Choi, Jin Eun,Jeon, Hyo Sung,Cha, Seung Ick,Cho, Sukki,Kim, Chang Ho,Park, Tae-In,Jung, Tae Hoon,Son, Ji-Woong,Park, Jae Yong The Korean Academy of Medical Sciences 2010 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.25 No.5
<P>Although <I>TP53</I> mutations have been widely studied in lung cancer, the majority of studies have focused on exons 5-8 of the gene. In addition, <I>TP53</I> mutations in Korean patients with lung cancers have not been investigated. We searched for mutations in the entire coding exons, including splice sites of the gene, in Korean patients with non-small cell lung cancer (NSCLC). Mutations of the gene were determined by direct sequencing in 176 NSCLCs. Sixty-nine mutations (62 different mutations) were identified in 65 tumors. Of the 62 mutations, 12 were novel mutations. <I>TP53</I> mutations were more frequent in males, ever-smokers and squamous cell carcinomas than in females, never-smokers and adenocarcinomas, respectively (all comparisons, <I>P</I><0.001). Missense mutations were most common (52.2%), but frameshift, nonsense, and splice-site mutations were frequently observed at frequencies of 18.8%, 15.9% and 10.1%, respectively. Of the 69 mutations, 9 (13.0%) were found in the oligomerization domain. In addition, the proportion of mutations in the oligomerization domain was significantly higher in adenocarcinomas than in squamous cell carcinomas (23.5% vs. 2.9%, <I>P</I>=0.01). Our study provides clinical and molecular characteristics of <I>TP53</I> mutations in Korean patients with NSCLCs.</P>
Lee, Seulki,Cha, Eui-Joon,Park, Kyeongsoon,Lee, Seung-Young,Hong, Jin-Ki,Sun, In-Cheol,Kim, Sang Yoon,Choi, Kuiwon,Kwon, Ick Chan,Kim, Kwangmeyung,Ahn, Cheol-Hee WILEY-VCH Verlag 2008 Angewandte Chemie Vol.47 No.15
<B>Graphic Abstract</B> <P>Nanodiagnosis: A matrix-metalloproteinase (MMP) sensitive gold-nanoparticle (AuNP) imaging probe quenches conjugated near-infrared (NIR) dyes with high efficiency and is specifically activated by the target MMPs (see picture, left). With this system, nanomolar amounts of protease can be detected—both in vitro and in vivo. Experiments disclose an apparent positive contrast in MMPs-positive tumor-bearing mice (right). <img src='wiley_img/14337851-2008-47-15-ANIE200705240-content.gif' alt='wiley_img/14337851-2008-47-15-ANIE200705240-content'> </P>
Lee, Eun Young,Lee, Hwan Young,Oh, Se Yoon,Jung, Sang-Eun,Yang, In Seok,Lee, Yang-Han,Yang, Woo Ick,Shin, Kyoung-Jin Elsevier 2016 FORENSIC SCIENCE INTERNATIONAL GENETICS Vol.22 No.-
<P><B>Abstract</B></P> <P>The application of next-generation sequencing (NGS) to forensic genetics is being explored by an increasing number of laboratories because of the potential of high-throughput sequencing for recovering genetic information from multiple markers and multiple individuals in a single run. A cumbersome and technically challenging library construction process is required for NGS. In this study, we propose a simplified library preparation method for mitochondrial DNA (mtDNA) analysis that involves two rounds of PCR amplification. In the first-round of multiplex PCR, six fragments covering the entire mtDNA control region and 22 fragments covering interspersed single nucleotide polymorphisms (SNPs) in the coding region that can be used to determine global haplogroups and East Asian haplogroups were amplified using template-specific primers with read sequences. In the following step, indices and platform-specific sequences for the MiSeq<SUP>®</SUP> system (Illumina) were added by PCR. The barcoded library produced using this simplified workflow was successfully sequenced on the MiSeq system using the MiSeq Reagent Nano Kit v2. A total of 0.4 GB of sequences, 80.6% with base quality of >Q30, were obtained from 12 degraded DNA samples and mapped to the revised Cambridge Reference Sequence (rCRS). A relatively even read count was obtained for all amplicons, with an average coverage of 5200×and a less than three-fold read count difference between amplicons per sample. Control region sequences were successfully determined, and all samples were assigned to the relevant haplogroups. In addition, enhanced discrimination was observed by adding coding region SNPs to the control region in <I>in silico</I> analysis. Because the developed multiplex PCR system amplifies small-sized amplicons (<250bp), NGS analysis using the library preparation method described here allows mtDNA analysis using highly degraded DNA samples.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We propose a simplified library preparation method composed of 2-round PCR amplification. </LI> <LI> mtDNA sequencing targeted the entire control region and 32 SNPs in the coding region. </LI> <LI> We obtained successful NGS results for 336 fragments from 12 degraded samples. </LI> <LI> Each sample was assigned to a certain haplogroup according to the observed sequence variation. </LI> <LI> NGS results of the control region corresponded to those using Sanger method. </LI> </UL> </P>