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Lim, Seung-Oe,Park, Sung-Gyoo,Yoo, Jun-Hi,Park, Young-Min,Kim, Hie-Joon,Jang, Kee-Taek,Cho, Jae-Won,Yoo, Byung-Chul,Jung, Gu-Hung,Park, Cheol-Keun WJG Press 2005 World journal of gastroenterology Vol.11 No.14
<P>Expression of heat shock proteins (HSPs) is frequently up-regulated in hepatocellular carcinoma (HCC), which evolves from dysplastic nodule (DN) and early HCC to advanced HCC. However, little is known about the differential expression of HSPs in multistep hepatocarcinogenesis. It was the purpose of this study to monitor the expression of HSPs in multistep hepatocarcinogenesis and to evaluate their prognostic significance in hepatitis B virus (HBV)-related HCC.</P>
Induction of Ovulation by Hormone Treatment in Ussurian Bullhead Leiocassis ussuriensis
Sang Gu Lim,Young Soo Kim,Hyung Kyun Han,Kwang Suk Kim,Dae Jung Kim,Eon Jong Kang,Sang Gyu Son,Eung Oh Kim 한국발생생물학회 2012 발생과 생식 Vol.16 No.4
The effects of ovulation induction in ussurian bullhead, Leiocassis ussuriensis, were investigated by treating ussurian bullhead with hCG, LHRHa, GnRHa, ovaprim, and pimozide. hCG was injected to ussurian bullhead at 0.75%NaCl, 5,000, 10,000, 20,000, and 30,000 IU, respectively. The ovulation inducement rates were 100% in 20,000 and 30,000IU. Fertilization rates were 82.7% and 79.8%. Hatching rates were 59.4% and 57.2%. Ovulation time was between 16-19hr The concentrations of LHRHa injected were 0.75 NaCl, 50, 100, 200, 300, and 300 μg/kg. The ovulation inducement rates were 100% in 300 and 400 ㎍/kg. Fertilization and hatching rates were 84.9% and 68.4% at 200 ㎍/kg. The times to ovulation were between 23 hr and 34 hr. Ovaprim of 0.75% NaCl, 1.0, 1.5, 2.0, 2.5 and 3.0 ml/kg were injected to the abdominal cavity. The ovulation inducement rate was highest at 2.0 and 3.0 ml/kg to 92% and ovulation time was between 27-38 hr. LHRHa concentrations of 0.75% NaCl, 50, 100, 200, 300 and 400 μg were injected with pimozide (1,000μg). Ovulation inducement rate was 100% from 200 to 400 IU with pimozide. Ovulation time was 22-36 h. Fertilization and hatching rates were 88.9% and 70.4% in 200 ㎍/kg with pimozide.
Sang Gu Lim,Hyoung Kyun Han,Hyun Woo Gil,박인석 한국발생생물학회 2012 발생과 생식 Vol.16 No.4
Korean bullhead (Pseudobagrus fulvidraco) was collected from the Kum River areas of Kangkyung-eup,Nonsan city, Chungcheongnam-do, Korea, from April to June, 2012 and was fertilized in order to observe egg development and temperature-related cleavage rates and mitotic intervals (τ0). The fertilized eggs were separative, demersal and light yellowish with 1.5±0.06 mm in diameter, and did not contain oil globules. The first cleavage stages were 90 min, 80 min,60 min and 50 min at 21℃, 24℃, 27℃ and 30℃, respectively. At higher temperatures, eggs developed faster and underwent further identical development. For Korean bullhead, τ0 were 33.4±2.08 min at 21℃, 31.5±3.06 min at 24℃, 28.1±2.11 min at 27℃ and 26.4±3.35 min at 30℃. There were strong negative correlations between the τ0 and water temperatures at all points studied (Y=-1.13X+58.15, R^2=0.98, n=30, where Y is τ0 and X is temperature). The results obtained in this work will be helpful for chromosome manipulation by use of cleavage frequency data and τ0 data in Korean bullhead.
The Effect of $H^+$ on Reduction of $[Co(NH_3)_4(C_2O_4)]^+$ with $[Fe(H_2O)_6]^{2+}$
Lim, Joo-Sang,Lee, Jae-Weon,Kang, Seung-Gu,Park, Byung-Kak Korean Chemical Society 1990 Bulletin of the Korean Chemical Society Vol.11 No.4
Kinetic studies were carried out for the redox reaction of $[Co(NH_3)_4(C_2O_4)]^+$ with aqueous $[Fe(H_2O)_6]^{2+}$ solution in the present of $H^+$ by UV/VIS-spectrophotometric method. It was found that the order of $H^+$ for the reaction is first one in the higher $H^+$ concentration range of $1.67×10^{-1} M{\sim}1.00 M,$ while second order in the lower range of $6.30×10^{-2} M{\sim}1.67{\times}10^{-1} M.$ Reaction order of the substrates was found to be first order with respect to each of them. Accordingly overall reactions are third or fourth order. The results of calculation for the Extended Huckel Molecular Orbital theory contribute to estimate the preferred intermediates, bridging form of binuclear complex. On the basis of these results, we propose that this redox reaction proceed via inner-sphere reaction mechanism.
Lim, Sang Gu,Han, Hyoung Kyun,Gil, Hyun Woo,Park, In-Seok The Korean Society of Developmental Biology 2012 발생과 생식 Vol.16 No.4
Korean bullhead (Pseudobagrus fulvidraco) was collected from the Kum River areas of Kangkyung-eup, Nonsan city, Chungcheongnam-do, Korea, from April to June, 2012 and was fertilized in order to observe egg development and temperature-related cleavage rates and mitotic intervals (${\tau}_0$). The fertilized eggs were separative, demersal and light yellowish with $1.5{\pm}0.06mm$ in diameter, and did not contain oil globules. The first cleavage stages were 90 min, 80 min, 60 min and 50 min at $21^{\circ}C$, $24^{\circ}C$, $27^{\circ}C$ and $30^{\circ}C$, respectively. At higher temperatures, eggs developed faster and underwent further identical development. For Korean bullhead, ${\tau}_0$ were $33.4{\pm}2.08$ min at $21^{\circ}C$, $31.5{\pm}3.06$ min at $24^{\circ}C$, $28.1{\pm}2.11$ min at $27^{\circ}C$ and $26.4{\pm}3.35$ min at $30^{\circ}C$. There were strong negative correlations between the $\tau_0$ and water temperatures at all points studied (Y=-1.13X+58.15, $R^2$=0.98, n=30, where Y is ${\tau}_0$ and X is temperature). The results obtained in this work will be helpful for chromosome manipulation by use of cleavage frequency data and ${\tau}_0$ data in Korean bullhead.
Lim, Sang Gu,Han, Hyoung Kyun,Kang, Jung Ha,Park, Hye Jung,Oh, Ji Su,Lim, Ji Su,Goo, In Bon,Park, In-Seok The Korean Society of Developmental Biology 2013 발생과 생식 Vol.17 No.3
Morphometric changes in the Ussurian bullhead, Leiocassis ussuriensis, and the Korean bullhead, Pseudobagrus fulvidraco, were observed during the early period of growth. Yolk length, yolk height, and yolk volume in the two species decreased within 9 days post-hatching (DPH) (p<0.05). The body lengths and body heights of both species increased gradually to 150 and 130 DPH, respectively (p<0.05). The horizontal distance between the anteriormost extension of the head and the anterior insertion of the pectoral fin, the anteriormost extension of the head ${\times}$ the verticality position of the anterior insertion of the primary dorsal fin rays, and the anterior insertion of the primary dorsal fin ${\times}$ the anterior insertion of the pectoral fin were greater in the Korean bullhead than in the Ussurian bullhead (p<0.05). However, the relative sizes of the head region, pectoral fin, ventral fin, and anal fin were greater in the Ussurian bullhead than in the Korean bullhead (p<0.05), and relative body depth and the size of the outer-mandible barbel were greater in the Korean bullhead than in the Ussurian bullhead (p<0.05). The growth curves of the morphometric characteristics of both species were divided into three types.