PRESERVATION OF MECHANICAL AND ENERGETIC FUNCTION AFTER ADENOVIRAL GENE TRANSFER IN NORMAL RAT HEARTS

Sakata, Susumu,Liang, Lifan,Sakata, Naoya,Sakata, Yuri,Chemaly, Elie R,Lebeche, Djamel,Takewa, Yoshiaki,Chen, Jiqiu,Park, Woo Jin,Kawase, Yoshiaki,Hajjar, Roger J Blackwell Publishing Asia 2007 Clinical and experimental pharmacology & physiolog Vol.34 No.12

<P>SUMMARY</P><OL TYPE='1'><li level='1'>The aim of the present study was to examine the acute and chronic effects of adenoviral gene transfer on cardiac function in terms of left ventricular (LV) mechanoenergetic function. Recombinant adenoviral vector carrying &bgr;-galactosidase and green fluorescent protein genes (Ad.&bgr;gal-GFP) was used. Cardiac function was examined in cross-circulated rat heart preparations, where end-systolic/diastolic pressure–volume relationships (ESPVR/EDPVR), systolic pressure–volume area (PVA), LV relaxation rate, equivalent maximal elastance at mid-range LV volume (eE<SUB>max</SUB> at mLVV), coronary blood flow, coronary vascular resistance and myocardial oxygen consumption (VO<SUB>2</SUB>) were also measured.</LI><li level='1'>To examine the <I>ex vivo</I> acute effects of the adenoviral vector, data were obtained before and 30–90 min after intracoronary infusion of Ad.&bgr;gal-GFP in the excised, cross-circulated hearts that underwent serotonin pretreatment. To examine the <I>in vivo </I>chronic effects of adenoviral gene transfer, normal rat hearts received Ad.&bgr;gal-GFP or saline by a catheter-based technique and data were obtained 3 days after the injection of Ad.&bgr;gal-GFP or saline.</LI><li level='1'>The ESPVR, EDPVR, LV relaxation rate, eE<SUB>max</SUB> at mLVV, coronary blood flow and coronary vascular resistance remained unchanged in Ad.&bgr;gal-GFP-transfected hearts in both <I>ex vivo</I> acute and <I>in vivo </I>chronic experiments. Moreover, the <I>ex vivo</I> and <I>in vivo </I>transfection caused no change in the slope and VO<SUB>2</SUB> intercept of the VO<SUB>2</SUB>–PVA relationship, VO<SUB>2</SUB> for basal metabolism and for Ca<SUP>2+</SUP> handling in excitation–contraction coupling and O<SUB>2</SUB> costs of LV contractility.</LI><li level='1'>These results indicate that adenoviral gene transfer has neither acute nor chronic toxic effects on LV mechanical and energetic function. A special combination of <I>in vivo </I>adenoviral gene transfer and a cross-circulation experimental system may provide a useful novel strategy to explore the functional and mechanoenergetic role of specifically targeted genes in the diseased heart.</LI></OL>

Education System to Learn the Skills of Management Decision-Making by Using Business Simulator with Speech Recognition Technology

Sakata, Daiki,Akiyama, Yusuke,Kaneko, Masaaki,Kumagai, Satoshi Korean Institute of Industrial Engineers 2014 Industrial Engineeering & Management Systems Vol.13 No.3

In this paper, we propose an educational system that involves a business game simulator and related curriculum. To develop these two elements, we examined the decision-making process related to business management and identified some significant skills thereby. In addition, we created an original simulator, named BizLator (http://bizlator.com), to help students develop these skills efficiently. Next, we developed a curriculum suitable for the simulator. We confirmed the effectiveness of the simulator and curriculum in a business-game-based class at Aoyama Gakuin University in Tokyo. On the basis of this, we compared our education system with a conventional system. This allowed us to identify advantages of and issues with our proposed system. Furthermore, we proposed a speech recognition support system named BizVoice in order to provide the teachers with more meaningful feedback, such as level of students' understanding. Concretely, BizVocie fetches students' speech of discussion during the game and converts the voice data to text data with speech recognition technology. Finally, teachers can grasp students' parameters of understanding, and thereby, the students also can take more effective class using BizLator. We also confirmed the effectiveness of the system in the class of Aoyama Gakuin Universiry.

Insect assemblages on flowering patches of 12 bamboo species

Sakata Yuzu,Kobayashi Keito,Makita Akifumi 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.3

Bamboos are known as long-living monocarpic plants that exhibit synchronous flowering at long intervals. It has been reported that florivory has a critical effect on their seed production in bamboos, especially in small scale flowering patches. In this study, we aimed to determine species composition and life history of florivorous insects in multiple bamboo species. We collected the inflorescences of 12 bamboo species from 15 sites in Japan from 2017 to 2019 and recorded insect assemblages found in the flowers. Five different insect species were observed to feed on the flowers of bamboo species. Among these, the larvae of two Dicraeus species were the most widely observed florivorous insects of the bamboo species. The other insects included the larvae of Cecidomyiidae sp., which was frequently observed on Sasa species, and the other two insects were Epuraea submicrurula, and Dimorphopterus japonicus. These insects were oviposited on the florets during the budding period. In addition, predators and parasitoids were found in the bamboo flowers. Our findings suggest that the bamboos flowers were mainly consumed by dipteran larvae. Insect species composition and frequency varied among bamboo species and sites. Comparing the frequency of the florivorous insects among bamboo flowering patches, including mass flowering patches, in different areas is needed in future studies.

영조법식 대목작과 부석사 무량수전과의 비교연구(2) 부재 : 중국영조법식과 한국전통건축과의 비교고찰 2

Sakata Izumi,문종만,박찬 대한건축학회 1992 大韓建築學會論文集 Vol.8 No.12

한국 전통 목조건축의 개구부 비례 특성에 관한 연구

Sakata Izumi,문종만 대한건축학회 1988 大韓建築學會論文集 Vol.4 No.5

Role of glycation and lipid peroxidation of low-density lipoprotein pathophysiology of diabetic angiopathy

Sakata, Noriyuki 仁濟大學校食品科學硏究所 2002 仁濟食品科學FORUM論叢 Vol.- No.9

Glycation of proteins and dyslipidemia contributes to the development of diabetic complications, including macro- and microangiopathies. Recently, glycation and oxidation reaction has been shown to share a common pathway, resulting in the formation of glycoxidative products, such as carboxy-methyllysine (CML) and pentosidine. In this study, we evaluated the role of glycation and lipid peroxidation of low-density lipoprotein (LDL) in the development of pathophysiology in diabetic angiopathy. Immunochemical analysis showed that CML was formed by in vitro glycoxidation of LDL, but not by oxidation and non-oxidative glycation of LDL. In contrast, malondialdehyde (MDA) formation was observed in both oxidation and glycoxidation of LDL, but not in non-oxidative glycation of LDL. Hexitol-lysine (HL), an Amadori product, was formed by non-oxidative glycation and glycoxidation of LDL, but not by oxidation of LDL. Immunohistochemical analysis showed that macrophage/foam cells in atherosclerotic and diabetic glomerular lesions accumulated glycoxidation and lipid peroxidation products, and expressed macrophage scavenger receptor class A. These results suggest that glycoxidation and lipid peroxidation of LDL synergistically promote the development of diabetic macro- and microangiopathies through interaction with macrophage scavenger receptor. However, there are various antioxidant agents in our body, which include superoxide dismutase, catalase and serum albumin. Human serum albumin (HSA) is the most abundant protein in the circulation and undergoes enhanced glycation and oxidation in diabetic patients. Therefore, decreased antioxidant activity of glycated and oxidized HSA may be involved in enhancement of lipid peroxidation of LDL in diabetic patients. We further examined the antioxidant activity of modified HSA to LDL oxidation. Native HSA showed a strong antioxidant activity to copper-induced oxidation of LDL, as evidenced by complete inhibition of MDA in the presence of more than 1 ㎎/ml of HSA. In contrast, in vitro glycoxidation induced the lowest antioxidant activity of HSA to LDL oxidation among various modifications. Oxidative HSA showed a moderate decrease in antioxidant activity. These results indicate that enhanced lipid peroxidation of LDL may be caused by not only glycoxidation of LDL, but also the decrease in the antioxidant activity of glycoxidative serum albumin. In conclusion, hyperglycemia in diabetic patients induces increased nonenzymatic chemical reaction between sugar and various proteins, and enhances oxidative stress. Consequently, glycoxidative and oxidative LDLs are increased in blood and accumulated in tissues. Macrophages incorporate these LDLs, and subsequently undergo cellular activation, leading to cell proliferation and matrix accumulation. These events may contribute to the development of diabetic macro- and micro-angiopathies.

영조 법식 대목작과 부석사 무량수전과의 비교연구 평면 및 단면 : 중국 영조 법식과 한국 전통 건축과의 비교고찰

Sakata Izumi,문종만,박찬 대한건축학회 1992 大韓建築學會論文集 Vol.8 No.5

A Design Method for Nonlinear Model Predictive Control with a Step-type Input Constraint

Ryuichi Sakata,Shiro Masuda,Takuya Sugiyama,Takao Sato 제어로봇시스템학회 2009 제어로봇시스템학회 국제학술대회 논문집 Vol.2009 No.8

The nonlinear model predictive control needs to solve a two-point boundary-value problem(TP-BVP) at every sample time based on the receding horizon control strategy. However, solving a nonlinear algebraic equation for the TP-BVP requires high computational load, so computing the cotrol law in real-time is a significant issue on the nonlinear model prective control. This paper, the refore, proposes a design method for nonlinear model predictive control with a step-type input constraint. The proposed method searches an optimal control law under a step-type input signal constraint. Hence, the number of unknown parameters in the nonlinear equation can bereduced to the number of inputs. The effectiveness of the proposed method is demonstrated through a numerical simulation.

Challenges in High-rise Wooden Structures and the Seismic Design in Japan

Hiroyasu, Sakata,Yoshihiro, Yamazaki Council on Tall Building and Urban Habitat Korea 2022 International journal of high-rise buildings Vol.11 No.3

Research and development on high-rise or large-scale wooden buildings have been actively conducted both domestically and internationally. The trend of high-rise wooden buildings is driven by increasing awareness of environmental issues. To utilize wooden materials in buildings is believed to lead to the reduction of the environmental impact. On the other hand, Japan is one of the most earthquake-prone countries in the world, and many wooden detached houses have been damaged in past major earthquakes. This paper summarizes the issues that arise in the realization of medium- and high-rise wooden buildings in Japan, and introduces the initiatives that have been seen so far.

Fault detection of shadow mask by use of image data processing

Masato Sakata,Hiroshi Kashiwagi 대한전자공학회 1992 대한전자공학회 학술대회 Vol.1992 No.10

At the KACC'91 conference, we proposed a method of automatic detection of shape of the faulty holes of a shadow mask which is used in a cathoderay tube of a color television. In this method, the image data are taken from two areas of the mask with CCD camera. Comparing the shape of holes in these two areas by use of a signal processing technique, we can find any fault in the shape of holes. This paper describes the effect of smoothing filters of effectively finding the faulty holes from the difference image data. A computer simulation and actual experiment with a shadow mask have shown that this method of fault detection is very effective for practical use.