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RISS 인기검색어
- 검색키워드
- 저자 : Sairam, Thiagarajan (검색결과 11 건)
- 무료
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Experimental study of graphene oxide on wollastonite induced cement mortar
Sairam, V.,Shanmugapriya, T.,Jain, Chetan,Agrahari, Himanshu Kumar,Malpani, Tanmay Techno-Press 2021 Advances in concrete construction Vol.12 No.6
Present research is mainly focused on, microstructural and durability analysis of Graphene Oxide (GO) in Wollastonite (WO) induced cement mortar with silica fume. The study was conducted by evaluating the mechanical properties (compressive and flexural strength), durability properties (water absorption, sorptivity and sulphate resistance) and microstructural analysis by SEM. Cement mortar mix prepared by replacing 10% ordinary portland cement with SF was considered as the control mix. Wollastonite replacement level varied from 0 to 20% by weight of cement. The optimum replacement of wollastonite was found to be 15% and this was followed by four sets of mortar specimens with varying substitution levels of cementitious material with GO at dosage rates of 0.1%, 0.2%, 0.3% and 0.4% by weight. The results indicated that the addition of up to 15%WO and 0.3% GO improves the hydration process and increase the compressive strength and flexural strength of the mortar due to the pore volume reduction, thereby strengthening the mortar mix. The resistance to water penetration and sulphate attack of mortar mixes were generally improved with the dosage of GO in presence of 15% Wollastonite and 10% silica fume content in the mortar mix. Furthermore, FE-SEM test results showed that the WO influences the lattice framework of the cement hydration products increasing the bonding between silica fume particles and cement. The optimum mix containing 0.3% GO with 15% WO replacement exhibited extensive C-S-H formation along with a uniform densified structure indicating that calcium meta-silicate has filled the pores.
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Design of Broadband Compact Canonical Triple-Sleeve Antenna Operating in UHF Band
Chandana SaiRam,Damera Vakula,Mada Chakravarthy 한국전자파학회JEES 2021 Journal of Electromagnetic Engineering and Science Vol.21 No.4
In this paper, a novel compact broadband antenna at UHF frequencies is presented with canonical shapes. Hemispherical, conical and cylindrical shapes have all been considered for antenna configuration. The designed antenna provides an instantaneous frequency range from 370 to 5,000 MHz with omnidirectional characteristics. The antenna was simulated in CST Microwave Studio, fabricated and evaluated; the results are presented. The simulated and measurement results are in good agreement. The antenna has voltage standing wave ratio (VSWR) ≤ 1.9:1 in 400–570 MHz, 2,530–3,740 MHz and 4,180–4,620 MHz; it has VSWR ≤ 3:1 over the operating frequency range 370–5,000 MHz and the measured gain varies from -0.6 to 4.5 dBi over the frequency band. The concept of canonical-shaped antenna elements and the incorporation of triple sleeves resulted in a reduction of the length of the antenna by 62% compared to the length of a half-wave dipole antenna designed at the lowest frequency. The antenna can be used for trans-receiving applications in wireless communication.
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Manoharan, Gomathi,Sairam, Thiagarajan,Thangamani, Rajesh,Ramakrishnan, Dhivya,K.Tiwari, Manish,Lee, Jung-Kul,Marimuthu, Jeya IPC Science and Technology Press 2019 Enzyme and microbial technology Vol.131 No.-
<P><B>Abstract</B></P> <P>Endophytic fungi provide benefits to host plants by producing a diverse class of secondary metabolites (natural products). Arrays of polyketide natural products are synthesized by specific classes of polyketide synthases (PKS I, II and III) in host organisms. In the present study, we attempt to screen and identify type III PKSs in culturable fungal endophytes isolated from the ethno medicinal plants including <I>Arbus precatorius</I>, <I>Bacopa monnieri,Citrus aurantifolia</I> and <I>Datura metel</I> to detect the genetic potential of endophytic fungi in producing bioactive compounds. A total of seventeen endophytic fungal strains belonging to eight genera were identified using fungal morphology and rDNA-ITS phylogenetic analyses. A CODEHOP-PCR based strategy was followed to design degenerate primers for the screening of type III PKS genes from fungal endophytes. We had successfully amplified partial PKS genes from eight endophytes. The amplified PKS sequences showed 60–99% identity to already characterized/putative PKS genes. From the partial sequence of FiPKS from <I>Fusarium incarnatum</I> BMER1, a full-length gene was amplified, cloned and characterized. FiPKScDNA was cloned and expressed in <I>E. coli</I> Lemo21 (DE3) and the purified protein was shown to produce pyrones and resorcinols using acyl-CoA thioesters as substrates. FiPKS showed the highest catalytic efficiency of 7.6 × 10<SUP>4</SUP> s<SUP>−1</SUP> M<SUP>-1</SUP> with stearoyl CoA as a starter unit. This study reports the identification and characterization of type III PKS from endophytes of medicinal plants by CODEHOP PCR.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A CODEHOP PCR based screening method was employed for type III polyketide synthase gene identification in fungal endophytes. </LI> <LI> By this approach, partial type III PKS genes from eight fungal endophytes were amplified and sequenced. </LI> <LI> FiPKS gene from Fusarium incarnatum BMER1, an endophyte of Bacopa monnieri was cloned and functionally characterized. </LI> <LI> FiPKS produced pyrones and resorcinols with the highest catalytic efficiency of 7.6 x 104 s-1 M-1towards stearoyl CoA. </LI> </UL> </P>
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Anand, V.,Sairam, N. Korea Information Processing Society 2017 Journal of information processing systems Vol.13 No.1
In this paper, we present an approach to transmit data from the source to the destination through a minimal path (least-cost path) in a computer network of n nodes. The motivation behind our approach is to address the problem of finding a minimal path between the source and destination. From the work we have studied, we found that a Steiner tree with bounded Steiner vertices offers a good solution. A novel algorithm to construct a Steiner tree with vertices and bounded Steiner vertices is proposed in this paper. The algorithm finds a path from each source to each destination at a minimum cost and minimum number of Steiner vertices. We propose both the sequential and parallel versions. We also conducted a comparative study of sequential and parallel versions based on time complexity, which proved that parallel implementation is more efficient than sequential.
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Gaurav Krishna,P Sairam Reddy,Pramod W Ramteke,Pogiri Rambabu,Kailas Bhagawanrao Tawar,Parthasarathi Bhattacharya 한국작물학회 2011 Journal of crop science and biotechnology Vol.14 No.3
Agrobacterium-mediated genetic transformation was performed using embryonic axes explants of pigeon pea. Both legume pod borer resistant gene (cry1Ac) and plant selectable marker neomycine phosphor transferase (nptII) genes under the constitutive expression of the cauliflower mosaic virus 35S promoter (CaMV35S) assembled in pPZP211 binary vector were used for the experiments. An optimum average of 44.61% successfully hardened dot blot Southern hybridization positive plants were obtained on co-cultivation media supplemented with 200 μM acetosyringone without L-cysteine. The increased transformation efficiency from a baseline of 11.53% without acetosyringone to 44.61% with acetosyringone was further declined with the addition of different concentrations of L-cysteine to co-cultivation media. Transgenic shoots were selected on 50 and 75 mg L^(-1) kanamycin. Rooting efficiency was 100%on half-strength Murashige and Skoog medium with 20 g L^(-1) sucrose and 0.5 mg L^(-1) indole butyric acid in the absence of kanamycin. Furthermore, 100% seed setting was found among all the transgenic events. The plants obtained were subjected to multi- and nochoice tests to determine the behavioral responses and mortality through Helicoverpa armigera bioassays on the leaf and relate their relationship with the expression of cry1Ac protein which was found to be less in leaf as compared to the floral buds, anther, pod, and seed.
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( V. Anand ),( N. Sairam ) 한국정보처리학회 2017 Journal of information processing systems Vol.13 No.1
In this paper, we present an approach to transmit data from the source to the destination through a minimal path (least-cost path) in a computer network of n nodes. The motivation behind our approach is to address the problem of finding a minimal path between the source and destination. From the work we have studied, we found that a Steiner tree with bounded Steiner vertices offers a good solution. A novel algorithm to construct a Steiner tree with vertices and bounded Steiner vertices is proposed in this paper. The algorithm finds a path from each source to each destination at a minimum cost and minimum number of Steiner vertices. We propose both the sequential and parallel versions. We also conducted a comparative study of sequential and parallel versions based on time complexity, which proved that parallel implementation is more efficient than sequential.
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Krishna, Gaurav,Reddy, P Sairam,Ramteke, Pramod W,Rambabu, Pogiri,Tawar, Kailas Bhagawanrao,Bhattacharya, Parthasarathi 한국작물학회 2011 Journal of crop science and biotechnology Vol.14 No.3
Agrobacterium-mediated genetic transformation was performed using embryonic axes explants of pigeon pea. Both legume pod borer resistant gene (crylAc) and plant selectable marker neomycine phosphor transferase (nptII) genes under the constitutive expression of the cauliflower mosaic virus 35S promoter (CaMV35S) assembled in pPZP211 binary vector were used for the experiments. An optimum average of 44.61% successfully hardened dot blot Southern hybridization positive plants were obtained on co-cultivation media supplemented with 200 ${\mu}M$ acetosyringone without L-cysteine. The increased transformation efficiency from a baseline of 11.53% without acetosyringone to 44.61% with acetosyringone was further declined with the addition of different concentrations of L-cysteine to co-cultivation media. Transgenic shoots were selected on 50 and 75 mg $L^{-1}$ kanamycin. Rooting efficiency was 100% on half-strength Murashige and Skoog medium with 20 g $L^{-1}$ sucrose and 0.5 mg $L^{-1}$ indole butyric acid in the absence of kanamycin. Furthermore, 100% seed setting was found among all the transgenic events. The plants obtained were subjected to multi- and nochoice tests to determine the behavioral responses and mortality through Helicoverpa armigera bioassays on the leaf and relate their relationship with the expression of cry1Ac protein which was found to be less in leaf as compared to the floral buds, anther, pod, and seed.
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Molecular characterization of two alkylresorcylic acid synthases from <i>Sordariomycetes</i> fungi
Ramakrishnan, Dhivya,Tiwari, Manish K.,Manoharan, Gomathi,Sairam, Thiagarajan,Thangamani, Rajesh,Lee, Jung-Kul,Marimuthu, Jeya Elsevier 2018 Enzyme and microbial technology Vol.115 No.-
<P><B>Abstract</B></P> <P>Two putative type III polyketide synthase genes (PKS) were identified from Sordariomycetes fungi. These two type III PKS genes from <I>Sordaria macrospora</I> (SmPKS) and <I>Chaetomium thermophilum</I> (CtPKS), shared 59.8% sequence identity. Both, full-length and truncated versions of type III PKSs were successfully cloned and overexpressed in a bacterial host, <I>Escherichia Coli</I> BL21 (DE3) using a N-terminus hexa-histidine tag. The full-length and the truncated construct of PKSs showed similar activity profiles, suggesting that additional amino acid residues at the C-terminal of both SmPKS and CtPKS may not be involved in catalytic functions. We demonstrate that these two recombinant polyketide synthases could efficiently synthesize tri- and tetraketide pyrones, resorcinols and resorcylic acids using various acyl-CoAs (C<SUB>4</SUB>–C<SUB>20</SUB>) as starter units. The truncated <I>S. macrospora</I> polyketide synthases (TrSmPKS) showed a maximum of 7.0 × 10<SUP>4</SUP> s<SUP>−1</SUP> M<SUP>−1</SUP> catalytic efficiency towards stearoyl-CoA.Whereas, truncated <I>C. thermophilum</I> polyketide synthases (TrCtPKS) preferred the long-chain acyl-CoA starter arachidoyl-CoA, to produce pentaketide and hexaketide resorcinols with a high catalytic efficiency of 6.2 × 10<SUP>4</SUP> s<SUP>−1</SUP> M<SUP>−1</SUP>. Homology model and substrate docking analyses suggest a shorter distance between sulfur of catalytic Cys152 and thioester carbonyl group of arachidoyl-CoA as well as stronger imidazolium–thiolate ion pair distance in TrCtPKS between catalytic Cys152-His309 compared to TrSmPKS- arachidoyl CoA complex. Enhanced binding interactions of CtPKS residues forming intermolecular contacts at the active site could be attributed to its high specificity towards arachidoyl-CoA. This study reports the functional characterization of two fungal type III polyketide synthases, SmPKS and CtPKS with high catalytic efficiency from S. <I>macrospora</I> and C. <I>thermophilum</I> respectively. Furthermore, the results suggested that the both SmPKS and CtPKS could be attractive targets for protein engineering to discern the unique substrate specificity and catalytic efficiency.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The study reports the functional characterization of two fungal type III polyketide synthases, SmPKS and CtPKS with high catalytic efficiency. </LI> <LI> Both the recombinant PKSs efficiently synthesize pyrones, resorcylic acids and resorcinols using various acyl- CoA (C<SUB>4</SUB>–C<SUB>20</SUB>) as starter units. </LI> <LI> TrCtPKS produces pentaketide and hexaketide resorcinols from arachidoyl-CoA with a high catalytic efficiency of 6.2 × 10<SUP>4</SUP> s<SUP>−1</SUP> M<SUP>−1</SUP>. </LI> <LI> Enhanced binding interactions of CtPKS residues forming intermolecular contacts at the active site could be attributed to its high specificity. </LI> </UL> </P>
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