Lack of TNF-α Gene Polymorphism (rs1799724) Association with Sustained Virological Response in Iranian Patients with Chronic HCV Infection

Larijani, Mona Sadat,Bahiraei, Narges,Nikbin, Mehri,Mohajel, Nasir,Rad, Leila Naghizadeh,Baghbani, Fahimeh,Mapar, Maryam,Sadat, Seyed Mehdi Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.8

Infection with the hepatitis C virus is a major public health concern which can lead to carcinoma and liver failure. It has been shown that single nucleotide polymorphisms can affect the level of gene activity of tumor necrosis factor (TNF) which has an important role, especially in viral infections which can lead to apaptosis of infected hepatocellular cells. We investigated the impact of three possible genotypes for rs1800629 or A/G single nucleotide polymorphism located downstream of $TNF{\alpha}$ gene promoter in groups of control (n=76) and chronic hepatitis C patients (n=89) focusing on the response to treatment among sensitive and resistant groups. Genomic DNA was extracted from $500{\mu}l$ prepheral whole blood and PCR and RFLP were used to amplify the region of interest and genotyping. With statistical analyzes a p-value <0.05 was considered meaningful. There was no significant difference in distribution of possible three genotypes among healthy individuals and patients (P=0.906, OR=1.194, CI=0.063-22.790). However, the frequency of G allele was higher in patients whereas A allele was more common among healthy individuals (p<0.0001). Further studies with more samples seem to be necessary.

Association of 8q24.21 rs10505477-rs6983267 Haplotype and Age at Diagnosis of Colorectal Cancer

Haerian, Monir Sadat,Haerian, Batoul Sadat,Rooki, Hassan,Molanaei, Saadat,Kosari, Farid,Obohhat, Maedeh,Hosseinpour, Parisa,Azimzadeh, Pedram,Mohebbi, Seyed Reza,Akbari, Zahra,Zali, Mohammad Reza Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.1

Background: Colorectal cancer (CRC) is the fourth most common cause of cancer death in the world. Genetic variants in 8q24.21 including rs10505477 and rs6983267 have been hypothesized to be involved in susceptibility to CRC. This study aims to investigate the possible association between these loci and their haplotypes with CRC risk in Iranian population. Materials and Methods: Subjects were recruited from two hospitals in Tehran. The rs10505477 and rs6983267 polymorphisms were genotyped by TaqMan real time PCR using subject genomic DNA, extracted either from formalin-fixed, paraffin-embedded tissue of patients or from blood of the controls by standard methods. Results: A total of 715 subjects (380 CRC patients and 335 matched controls) were genotyped in this study. Allele and genotype analysis of the rs10505477 and rs6983267 polymorphisms by gender, age at diagnosis, tumor location, tumor grade, and tumor node metastasis (TNM) showed no significant association with CRC risk. There was a significant relationship between GG haplotype and susceptibility to age at diagnosis for both <60 and ${\geq}60$ (p=0.0005 and p=0.000004, respectively) and between GT and CRC in the age at diagnosis ${\geq}60$ (Table 3: p=0.031). The GG haplotype was less frequent in CRC patients with the age at diagnosis <60, but was more common in subjects with the age at diagnosis ${\geq}60$. Conclusions: Results of this study suggests that the rs6983267 and rs10505477 polymorphisms alone may not be relevant to CRC risk, but their GG haplotype plays a notable role in age at diagnosis of CRC in the Iranian population.

URANS simulations for a flooded ship in calm water and regular beam waves

Sadat-Hosseini, H.,Kim, D.H.,Carrica, P.M.,Rhee, S.H.,Stern, F. Pergamon Press 2016 Ocean engineering Vol.120 No.-

CFD simulations are conducted for zero-speed damaged passenger ship SSRC in calm water and waves with 6DOF motions including flooding procedure in calm water, roll decay in calm water and motions in regular beam waves for various wavelengths. The simulations model the 6DOF soft spring experimental mount, the one- and two-room flooding compartment configurations, including both intact and damaged conditions. For flooding and roll decay, simulations show ability to predict the trend of increases in roll period and damping due to flooding, as reported in ITTC. The damping magnitudes were often under-predicted with large errors while the roll period and compartment water height were well predicted. Two-room compartment simulation showed three times larger damping than one-room compartment cases whereas the roll period was similar for both conditions. For wave cases, all motions show primarily 1st order responses, except for parametric roll condition which shows large ½ harmonic responses for the intact ship. The 2nd order responses are small for both damaged and intact ship. The larger roll period and damping for the damaged ship shift the peak of responses to smaller wave frequency and reduce the amplitude of responses. The average error is often large for 1st order intact ship pitch and damaged ship surge and pitch. The errors are larger for most ½ and 2nd order responses. Large errors could be partially due to the complex mounting system in the experiment. Overall, current CFD results show better predictions than those reported for potential flow solvers even though the computational cost is larger.

A Novel Production Method for High-Fructose Glucose Syrup from Sucrose-Containing Biomass by a Newly Isolated Strain of Osmotolerant Meyerozyma guilliermondii

( Sadat Mohammad Rezq Khattab ),( Tsutomu Kodaki ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.4

One osmotolerant strain from among 44 yeast isolates was selected based on its growth abilities in media containing high concentrations of sucrose. This selected strain, named SKENNY, was identified as Meyerozyma guilliermondii by sequencing the internal transcribed spacer regions and partial D1/D2 large-subunit domains of the 26S ribosomal RNA. SK-ENNY was utilized to produce high-fructose glucose syrup (HFGS) from sucrose-containing biomass. Conversion rates to HFGS from 310-610 g/l of pure sucrose and from 75-310 g/l of sugar beet molasses were 73.5-94.1% and 76.2-91.1%, respectively. In the syrups produced, fructose yields were 89.4-100% and 96.5-100% and glucose yields were 57.6-82.5% and 55.3-79.5% of the theoretical values for pure sucrose and molasses sugars, respectively. This is the first report of employing M. guilliermondii for production of HFGS from sucrose-containing biomass.

Wheat Blast: A New Fungal Inhabitant to Bangladesh Threatening World Wheat Production

Sadat, Md. Abu,Choi, Jaehyuk The Korean Society of Plant Pathology 2017 Plant Pathology Journal Vol.33 No.2

World wheat production is now under threat due to the wheat blast outbreak in Bangladesh in early March 2016. This is a new disease in this area, indicating the higher possibility of this pathogen spreading throughout the Asia, the world's largest wheat producing area. Occurrence of this disease caused ~3.5% reduction of the total wheat fields in Bangladesh. Its economic effect on the Bangladesh wheat market was little because wheat contributes to 3% of total cereal consumption, among which ~70% have been imported from other countries. However, as a long-term perspective, much greater losses will occur once this disease spreads to other major wheat producing areas of Bangladesh, India, and Pakistan due to the existing favorable condition for the blast pathogen. The wheat blast pathogen belongs to the Magnaporthe oryzae species complex causing blast disease on multiple hosts in the Poaceae family. Phylogenetic analysis revealed that the Bangladesh outbreak strains and the Brazil outbreak strains were the same phylogenetic lineage, suggesting that they might be migrated from Brazil to Bangladesh during the seed import. To protect wheat production of Bangladesh and its neighbors, several measures including rigorous testing of seed health, use of chemicals, crop rotation, reinforcement of quarantine procedures, and increased field monitoring should be implemented. Development of blast resistant wheat varieties should be a long-term solution and combination of different methods with partial resistant lines may suppress this disease for some time.

Analysis of in planta Expressed Orphan Genes in the Rice Blast Fungus Magnaporthe oryzae

Sadat, Md. Abu,Jeon, Junhyun,Mir, Albely Afifa,Kim, Seongbeom,Choi, Jaeyoung,Lee, Yong-Hwan The Korean Society of Plant Pathology 2014 Plant Pathology Journal Vol.30 No.4

Genomes contain a large number of unique genes which have not been found in other species. Although the origin of such "orphan" genes remains unclear, they are thought to be involved in species-specific adaptive processes. Here, we analyzed seven orphan genes (MoSPC1 to MoSPC7) prioritized based on in planta expressed sequence tag data in the rice blast fungus, Magnaporthe oryzae. Expression analysis using qRT-PCR confirmed the expression of four genes (MoSPC1, MoSPC2, MoSPC3 and MoSPC7) during plant infection. However, individual deletion mutants of these four genes did not differ from the wild-type strain for all phenotypes examined, including pathogenicity. The length, GC contents, codon adaptation index and expression during mycelial growth of the four genes suggest that these genes formed during the evolutionary history of M. oryzae. Synteny analyses using closely related fungal species corroborated the notion that these genes evolved de novo in the M. oryzae genome. In this report, we discuss our inability to detect phenotypic changes in the four deletion mutants. Based on these results, the four orphan genes may be products of de novo gene birth processes, and their adaptive potential is in the course of being tested for retention or extinction through natural selection.

Parametric Study of FRP Strengthening on Stress Concentration Factors in an Off shore Tubular T-Joint Subjected to In-Plane and Out-of-Plane Bending Moments

Alireza Sadat Hosseini,Mohammad Reza Bahaari,Mohammad Lesani 한국강구조학회 2019 International Journal of Steel Structures Vol.19 No.6

This paper describes numerical investigations conducted on a tubular T-joint strengthened by FRP composites under in-plane bending (IPB) and out-of-plane bending (OPB) moments. The FRP materials were applied as a protective layer on the T-joint, and the analyzed models were eventually validated against the results obtained from an experimental study. The eff ectiveness of using FRP materials on enhancing the fatigue life of a tubular T-joint was investigated through computing the relative SCFs of 150 fi nite element models using ABAQUS software package. Analyses have been performed to investigate diff erent FRP parameters such as fi ber orientation, FRP thickness, wrapping length and FRP material properties of the wrapped joint. Eff ectiveness of these FRP parameters on SCF reduction along with the most effi cient lay-ups against both IPB and OPB moments were reported. According to the results of this study, further reduction in SCFs was observed when the joint was confi ned with stiff er FRPs. SCF reduction of around 15% was observed for only a 1 mm thick CFRP layup. Moreover, using a thicker FRP layup tends to increase its eff ectiveness signifi cantly. However, it was observed that increasing the FRP wrapping length has negligible eff ect on SCF values, and has to be chosen so as to cover all the chord surface area infl uenced by the brace loading in order to balance the state of stresses and deformations.

A new algorithm for design of support structures in additive manufacturing by using topology optimization

Haleh Sadat Kazemi,Seyed Mehdi Tavakkoli 국제구조공학회 2023 Structural Engineering and Mechanics, An Int'l Jou Vol.86 No.1

In this paper, a density based topology optimization is proposed for generating of supports required in additive manufacturing to maintain the overhanging regions of main structures during layer by layer fabrication process. For this purpose, isogeometric analysis method is employed to model geometry and structural analysis of main and support structures. In order to model the problem two cases are investigated. In the first case, design domain of supports can easily be separated from the main structure by using distinct isogeometric patches. The second case happens when the main structure itself is optimized by using topology optimization and the supports should be designed in the voids of optimum layout. In this case, in order to avoid boundary identification and re-meshing process for separating design domain of supports from main structure, a parameterization technique is proposed to identify the design domain of supports. To achieve this, two density functions are defined over the entire domain to describe the main structure and supporting areas. On the other hand, since supports are under gravity loads while main structure and its stiffness is not completed during manufacturing process, in the proposed method, stiffness of the main structure is considered to be trivial and the gravity loads are also naturally applied to design support structures. By doing so, the results show reasonable supports are created to protect, continuously, overhanging surfaces of the main structure. Several examples are presented to demonstrate the efficiency of the proposed method and compare the results with literature.

Evaluating Kyphosis and Lordosis in Students by Using a Flexible Ruler and Their Relationship with Severity and Frequency of Thoracic and Lumbar Pain

Sedigheh-Sadat Mirbagheri,Amir Rahmani-Rasa,Farzad Farmani,Payam Amini,Mohammad-Reza Nikoo 대한척추외과학회 2015 Asian Spine Journal Vol.9 No.3

Study Design: A cross-sectional, descriptive study. Purpose: This study aimed to investigate the relationship between kyphosis and lordosis measured by using a flexible ruler and musculoskeletal pain in students of Hamadan University of Medical Sciences. Overview of Literature: The spine supports the body during different activities by maintaining appropriate body alignment and posture. Normal alignment of the spine depends on its structural, muscular, bony, and articular performance. Methods: Two hundred forty-one students participated in this study. A single examiner evaluated the angles of lumbar lordosis and thoracic kyphosis by using a flexible ruler. To determine the severity and frequency of pain in low-back and inter-scapular regions, a tailor-made questionnaire with visual analog scale was used. Finally, using the Kendall correlation coefficient, the data were statistically analyzed. Results: The mean value of lumbar lordosis was 34.46°±12.61° in female students and 22.46°±9.9° in male students. The mean value of lumbar lordosis significantly differed between female and male students (p <0.001). However, there was no difference in the level of the thoracic curve (p =0.288). Relationship between kyphosis measured by using a flexible ruler and inter-scapular pain in male and female students was not significant (p =0.946). However, the relationship between lumbar lordosis and low back pain was statistically significant (p =0.006). Also, no significant relationship was observed between abnormal kyphosis and frequency of inter-scapular pain, and between lumbar lordosis and low back pain. Conclusions: Lumbar lordosis contributes to low back pain. The causes of musculoskeletal pain could be muscle imbalance and muscle and ligament strain.

Synthesis and characterization of mercury telluride nanoparticles using a new precursor

Fatemeh Sadat Sangsefidi,Masoud Salavati-Niasari,Mahdiyeh Esmaeili-Zare 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.5

HgTe nanoparticles have been synthesized through a simple hydrothermal reduction approach using [NN0-bis(salicylaldehydo)ethylenediamine]mercury(II), [Hg(salen)] complex, as a new precursor. HgTe nanoparticles with size about 15–25 nm was prepared. The products were also characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), photoluminescence spectroscopy (PL), X-ray energy dispersive spectroscopy (EDS) and Fourier transform infrared (FT-IR) spectra. Effects of temperature, reaction time and reductant agent on the morphology, the particle sizes and the phase of the final products have been investigated.