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Chemical transformation and target preparation of saponins in stems and leaves of Panax notoginseng
Wang, Ru-Feng,Li, Juan,Hu, Hai-Jun,Li, Jia,Yang, Ying-Bo,Yang, Li,Wang, Zheng-Tao The Korean Society of Ginseng 2018 Journal of Ginseng Research Vol.42 No.3
Background: Notoginsenoside Ft1 is a promising potential candidate for cardiovascular and cancer disease therapy owing to its positive pharmacological activities. However, the yield of Ft1 is ultralow utilizing reported methods. Herein, an acid hydrolyzing strategy was implemented in the acquirement of rare notoginsenoside Ft1. Methods: Chemical profiles were identified by ultraperformance liquid chromatography coupled with quadruple-time-of-flight and electrospray ionization mass spectrometry (UPLC-Q/TOF-ESI-MS). The acid hydrolyzing dynamic changes of chemical compositions and the possible transformation pathways of saponins were monitored by ultrahigh-performance LC coupled with tandem MS (UHPLC-MS/ MS). Results and conclusion: Notoginsenoside Ft1 was epimerized from notoginsenoside ST4, which was generated through cleaving the carbohydrate side chains at C-20 of notoginsenosides Fa and Fc, and vinaginsenoside R7, and further converted to other compounds via hydroxylation at C-25 or hydrolysis of the carbohydrate side chains at C-3 under the acid conditions. High temperature contributed to the hydroxylation reaction at C-25 and 25% acetic acid concentration was conducive to the preparation of notoginsenoside Ft1. C-20 epimers of notoginsenoside Ft1 and ST4 were successfully separated utilizing solvent method of acetic acid solution. The theoretical preparation yield rate of notoginsenoside Ft1 was about 1.8%, which would be beneficial to further study on its bioactivities and clinical application.
Intense X-ray induced formation of silver nanoparticles stabilized by biocompatible polymers
Wang, Chang-Hai,Liu, Chi-Jen,Wang, Cheng-Liang,Chien, Chia-Chi,Hwu, Y.,Liu, Ru-Shi,Yang, Chung-Shi,Je, Jung-Ho,Lin, Hong-Ming,Margaritondo, G. Springer-Verlag 2009 Applied physics. A, Materials science & processing Vol.97 No.2
Wang, Fu-Ru,Fang, Qiao-Qiao,Tang, Wei-Ming,Xu, Xiao-San,Mahapatra, Tanmay,Mahapatra, Sanchita,Liu, Yu-Fei,Yu, Ning-Le,Sun, Quan-Fu Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.11
Medical diagnostic X-ray workers are one occupational group that expose to the long-term low-dose external radiation over their working lifetime, and they may under risk of different cancers. This study aims to determine the relationship between the occupational X-ray radiation exposure and cancer risk among these workers in Jiangsu, China. We conducted Nested case-control study to investigate the occupational X-ray radiation exposure and cancer risk. Data were collected through self-administered questionnaire, which includes but not limits to demographic data, personal behaviors and family history of cancer. Retrospective dose reconstruction was conducted to estimate the cumulative doses of the x-ray workers. Inferential statistics, t-test and 2 tests were used to compare the differences between each group. We used the logistic regression model to calculate the odds ratio (OR) and 95% confidence interval (CI) of cancer by adjusting the age, gender. All 34 breast cancer cases and 45 esophageal cancer cases that detected in a cohort conducted among health workers between 1950~2011 were included in this presented study, and 158 cancer-free controls were selected by frequency-matched (1:2). Our study found that the occupational radiation exposure was associated with a significantly increased cancer risk compared with the control, especially in breast cancer and esophageal cancer (adjusted OR=2.90, 95% CI: 1.19-7.04 for breast cancer; OR=4.19, 95% CI: 1.87-9.38 for esophageal cancer, and OR=3.43, 95% CI: 1.92-6.12 for total cancer, respectively). The occupational X-ray radiation exposure was associated with increasing cancer risk, which indicates that proper intervention and prevention strategies may be needed in order to bring down the occupational cancer risk.
( Ru Wang ),( Chen Wang ),( Qing Feng ),( Rey-may Liou ),( Ying-feng Lin ) 한국미생물생명공학회(구 한국산업미생물학회) 2021 Journal of microbiology and biotechnology Vol.31 No.3
Using salt-tolerant bacteria to protect plants from salt stress is a promising microbiological treatment strategy for saline-alkali soil improvement. Here, we conducted research on the growth-promoting effect of Brevibacterium frigoritolerans on wheat under salt stress, which has rarely been addressed before. The synergistic effect of B. frigoritolerans combined with representative salt-tolerant bacteria Bacillus velezensis and Bacillus thuringiensis to promote the development of wheat under salt stress was also further studied. Our approach involved two steps: investigation of the plant growth-promoting traits of each strain at six salt stress levels (0, 2, 4, 6, 8, and 10%); examination of the effects of the strains (single or in combination) inoculated on wheat in different salt stress conditions (0, 50, 100, 200, 300, and 400 mM). The experiment of plant growth-promoting traits indicated that among three strains, B. frigoritolerans had the most potential for promoting wheat parameters. In single-strain inoculation, B. frigoritolerans showed the best performance of plant growth promotion. Moreover, a pot experiment proved that the plant growth-promoting potential of co-inoculation with three strains on wheat is better than single-strain inoculation under salt stress condition. Up to now, this is the first report suggesting that B. frigoritolerans has the potential to promote wheat growth under salt stress, especially combined with B. velezensis and B. thuringiensis.
Yingying Wang,Bin Yang,Liangyong He,Yuqing Yang,Nuo Zhang,Yang Wang,Zhiqiang Shi,Yuchao Ke,Lifen Su,Jia-Sheng Qian,RU XIA,Tao Jiang 한국고분자학회 2022 폴리머 Vol.46 No.5
In this study, a series of poly(methyl methacrylate) (PMMA) copolymer films were prepared via solutionpolymerization of methyl methacrylate (MMA) with butyl acrylate (BA) and lauryl methacrylate (LMA) as monomers. Mechanical properties, hydrophobic properties, and optical properties of the films were intensively investigated. The rheological results showed that the fluidity of the copolymer was considerably enhanced. When the monomer ratio ofMMA:BA:LMA was 100:30:10, the copolymer film S4 showed the best overall performance with perfect optical transparency maintained. The results of the dynamic mechanical and thermal analysis suggested that the glass transition temperature (Tg) moved towards lower temperature, with enhanced ductility of the PMMA films. A large number of yieldfolds and crazes appeared on the cross-sectional surface of copolymer films through morphological observations, displaying the obvious characteristics of toughness fracture and obeying the energy dissipation mechanism of cracks shearband. The present study provided a facile way of preparing PMMA films with high toughness and light transmittanceby appropriate selection of the monomers, which will be of practical significance for further studies on the replacementof triacetyl cellulose as a support film of polarizers.
Zeng-ru Zhao,Xin Wang,Xue-feng Zhang,Qiang Ma,Yan-li Liu,Yong-feng Li,Fei Liu,Gao-feng Wang 한국자기학회 2017 Journal of Magnetics Vol.22 No.1
Mischmetal of Bayan Obo ore was utilized to prepare the high performance (Pr7.34Nd21.86)1-xMMxFe68.7Al0.1-Cu0.12Co0.88B ribbons using melt-spinning method. Phase composition and magnetic properties were investigated at room temperature. The ribbons mainly consist of R2Fe14B phase in isotropic nanostructure. Both coercivity and maximum energy product decrease with the increase of MM content. The magnetic parameters of the ribbons with MM = 20 % in mass are Br = 7.38 kGs, Hcj = 13.66 kOe, (BH)max = 11.81 MGOe. Henkel plots were applied to demonstrate the exchange coupling interaction between grains.
Xu, Ru-Xiang,Wei, Ning,Wang, Yu,Wang, Guo-Qiang,Yang, Gong-She,Pang, Wei-Jun Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.9
Lymphoid enhancer binding factor 1 (LEF-1) is a member of the T-cell specific factor (TCF) family, which plays a key role in the development of breast endothelial cells. Moreover, LEF-1 gene has been identified as a candidate gene for teat number trait. In the present study, we detected two novel mutations (NC_010450.3:g. 99514A>G, 119846C>T) by DNA sequencing and polymerase chain reaction-restriction fragment length polymorphism in exon 4 and intron 9 of LEF-1 in Guanzhong Black, Hanjiang Black, Bamei and Large White pigs. Furthermore, we analyzed the association between the genetic variations with teat number trait in these breeds. The 99514A>G mutation showed an extremely significant statistical relevance between different genotypes and teat number trait in Guanzhong (p<0.001) and Large White (p = 0.002), and significant relevance in Hanjiang (p = 0.017); the 119846C>T mutation suggested significant association in Guanzhong Black pigs (p = 0.042) and Large White pigs (p = 0.003). The individuals with "AG" or "GG" genotype displayed more teat numbers than those with "AA"; the individuals with "TC" or "CC" genotype showed more teat numbers than those with "TT". Our findings suggested that the 99514A>G and 119846C>T mutations of LEF-1 affected porcine teat number trait and could be used in breeding strategies to accelerate porcine teat number trait improvement of indigenous pigs breeds through molecular marker assisted selection.