Evaluating phylogenetic relationships in the Lilium family using the ITS marker

Ghanbari, Sina,Fakheri, Barat Ali,Naghavi, Mohammad Reza,Mahdinezhad, Nafiseh The Korean Society of Plant Biotechnology 2018 식물생명공학회지 Vol.45 No.3

Lilium is a perennial bulbous plant belonging to the liriotypes genus. Our aim was to study the phylogenetic relationships of the Lilium family. Two varieties of Lilium ledebourii, 44 varieties of the gene bank, and one variety from the Tulipa family served as the out group. In order to study the diversity between lilium masses, ITS regions were used to design the marker. The results showed that the guanine base is the most abundant nucleotide. Relatively high conservation was observed in the ITS regions of the populations (0.653). Phylogenetic analysis showed that sargentiae and hybrid varieties are older than other varieties of the Lilium family. Also, the location of L. ledebourii varieties (Damash and Namin) was identified in a phylogenetic tree by using the ITS marker. Overall, our research showed that ITS molecular markers are very suitable for phylogenetic studies in the Lilium family.

New protocol for the indirect regeneration of the Lilium ledebourii Bioss by using bulb explants

Ghanbari, Sina,Fakheri, Barat Ali,Naghavi, Mohammad Reza,Mahdinezhad, Nafiseh The Korean Society of Plant Biotechnology 2018 식물생명공학회지 Vol.45 No.2

Lilium ledebourii Bioss is a wild species of Lilium, which grows naturally in some provinces of Iran. Previous studies on Lilium tissue culture have been linked to direct regeneration and a few studies have been conducted on indirect regeneration, which has been studied under bright conditions. In this study, for the first time in the world, all the stages of indirect regeneration (callus induction, shoot and root induction) have been studied under dark conditions. Callus formation and the regeneration levels of L. Ledebourii Bioss were examined for three replicates in an MS (Murashige and Skoog) medium with different hormonal compositions and by using a factorial experiment in the framework of a completely random plan. For callus initiation, 2,4-D and kinetin hormones were used in five and four levels, respectively, as auxin and cytokinin. Results showed that the highest percentage of the callus was found in $3{\mu}M$ of 2,4-D and $0.5{\mu}M$ of kinetin. In terms of callus wet weight, the highest amount was found in $3{\mu}M$ of 2,4-D and $0.5{\mu}M$ of kinetin. In addition, in terms of diameter, the highest amount was found in $3{\mu}M$ of 2,4-D, and $0.5{\mu}M$ of kinetin. In summary, the 2,4-D hormone had a major impact on the percentage of regeneration increase so that the best response was related to the composition of $3{\mu}M$ of 2,4-D, and $0.1{\mu}M$ of kinetin. This study contended that auxin and cytokinin can induce long shoots and roots through cell elongation in dark condition.

Design and Performance of an Integrated High Step-up Boost-Cuk-Forward Converter

Reza Heidari(헤이다리 레자),Mohammad Ali Ghanbari(감바리 모하마드 알리),Ehsan Adib(아디브 에산),Kwang-Il Jeong(정광일),Jin-Woo Ahn(안진우) 대한전기학회 2023 대한전기학회 학술대회 논문집 Vol.2023 No.10

Evaluating phylogenetic relationships in the Lilium family using the ITS marker

Sina Ghanbari,Barat Ali Fakheri,Mohammad Reza Naghavi,Nafiseh Mahdinezhad 한국식물생명공학회 2018 JOURNAL OF PLANT BIOTECHNOLOGY Vol.45 No.3

Lilium is a perennial bulbous plant belonging to the liriotypes genus. Our aim was to study the phylogenetic relationships of the Lilium family. Two varieties of Lilium ledebourii, 44 varieties of the gene bank, and one variety from the Tulipa family served as the out group. In order to study the diversity between lilium masses, ITS regions were used to design the marker. The results showed that the guanine base is the most abundant nucleotide. Relatively high conservation was observed in the ITS regions of the populations (0.653). Phylogenetic analysis showed that sargentiae and hybrid varieties are older than other varieties of the Lilium family. Also, the location of L. ledebourii varieties (Damash and Namin) was identified in a phylogenetic tree by using the ITS marker. Overall, our research showed that ITS molecular markers are very suitable for phylogenetic studies in the Lilium family.

New protocol for the indirect regeneration of the Lilium ledebourii Bioss by using bulb explants

Sina Ghanbari,Barat Ali Fakheri,Mohammad Reza Naghavi,Nafiseh Mahdinezhad 한국식물생명공학회 2018 JOURNAL OF PLANT BIOTECHNOLOGY Vol.45 No.2

Lilium ledebourii Bioss is a wild species of Lilium, which grows naturally in some provinces of Iran. Previous studies on Lilium tissue culture have been linked to direct regeneration and a few studies have been conducted on indirect regeneration, which has been studied under bright conditions. In this study, for the first time in the world, all the stages of indirect regeneration (callus induction, shoot and root induction) have been studied under dark conditions. Callus formation and the regeneration levels of L. Ledebourii Bioss were examined for three replicates in an MS (Murashige and Skoog) medium with different hormonal compositions and by using a factorial experiment in the framework of a completely random plan. For callus initiation, 2,4-D and kinetin hormones were used in five and four levels, respectively, as auxin and cytokinin. Results showed that the highest percentage of the callus was found in 3 μM of 2,4-D and 0.5 μM of kinetin. In terms of callus wet weight, the highest amount was found in 3 μM of 2,4-D and 0.5 μM of kinetin. In addition, in terms of diameter, the highest amount was found in 3 μM of 2,4-D, and 0.5 μM of kinetin. In summary, the 2,4-D hormone had a major impact on the percentage of regeneration increase so that the best response was related to the composition of 3 μM of 2,4-D, and 0.1 μM of kinetin. This study contended that auxin and cytokinin can induce long shoots and roots through cell elongation in dark condition.

Gastric cancer and mesenchymal stem cell-derived exosomes: from pro-tumorigenic effects to anti-cancer vehicles

Maryam Dolatshahi,Ahmad Reza Bahrami,Qaiser Iftikhar Sheikh,Mohsen Ghanbari,Maryam M. Matin 대한약학회 2024 Archives of Pharmacal Research Vol.47 No.1

Gastric cancer (GC) is one of the most prevalent malignancies in the world, with a high mortality rate in both womenand men. Conventional treatments, like chemotherapy, radiotherapy and surgery, are facing some drawbacks like acquireddrug resistance and various side eff ects, leading to cancer recurrence and increased morbidity; thus, development of novelapproaches in targeted therapy would be very benefi cial. Exosomes, extracellular vesicles with a size distribution of sub-150 nm, interplay in physiological and pathophysiological cell–cell communications and can pave the way for targeted cancertherapy. Accumulating pieces of evidence have indicated that exosomes derived from mesenchymal stem cells (MSC-EXs)can act as a double-edged sword in some cancers. The purpose of this review is to assess the diff erences between stem celltherapy and exosome therapy. Moreover, our aim is to demonstrate how naïve MSCs transform into GC-MSCs in the tumormicroenvironment. Additionally, the tumorigenic and anti-proliferation eff ects of MSC-EXs derived from diff erent originswere investigated. Finally, we suggest potential modifi cations and combination options that involve utilizing MSC-EXs fromthe foreskin and umbilical cord as promising sources to enhance the effi cacy of gastric cancer treatment. This approach ispresented in contrast to bone marrow cells, which are more heterogeneous, age-related, and are also easily aff ected by thepatient's circulation system.

Photocatalytic degradation of organic dyes using WO3-doped ZnO nanoparticles fixed on a glass surface in aqueous solution

Roya Ebrahimi,Afshin Maleki,Yahya Zandsalimi,Reza Ghanbari,Behzad Shahmoradi,Reza Rezaee,Mahdi Safari,주상우,Hiua Daraei,Shivaraju Harikaranahalli Puttaiah,Omid Giahi 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.73 No.-

The present study aimed at evaluating the application of tungsten oxide-doped zinc oxide nanoparticlesfor the photocatalytic degradation of Direct Blue 15 dye in a sequencing batch reactor. ZnO nanoparticleswere doped with WO3 through hydrothermal synthesis method. To characterize the synthesizednanoparticles scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy,atomic force microscopy, zeta potential analysis, and ultraviolet–visible spectroscopy were used. Theradiation source in this study wasfive 6 W UV lamps. Operational parameters affecting the process,namely pH, light intensity, dopant percentage, dye concentration, and contact time, were evaluated. Theresults of the present study revealed that the efficiency of the photocatalytic process for the degradationof organic dyes was higher at acidic pH values than neutral or basic values. In addition, upon increasingthe light intensity from 172 to 505 W/m2, the efficacy of dye degradation was enhanced from 27.8 to73.5%. Increasing the concentration of the dopant percentage from 1 to 5% w/v increased the degradationefficacy from 30.69 to 73.1%. Increasing the initial dye concentration from 20 to 100 mg/L decreased thedegradation efficacy from 86.9 to 37.5%. Photocatalytic process using WO3-doped ZnO nanoparticlesfixedon a glass surface thus was proven to show a good efficiency for the degradation of organic dye in aquaticsolutions.

Association of Vitamin D status with Visceral Adiposity Index and Lipid Accumulation Product Index among a Group of Iranian People

( Elham Bazshahi ),( Sanaz Pourreza ),( Mahtab Ghanbari ),( Zeinab Khademi ),( Mohammad Reza Amini ),( Kurosh Djafarian ),( Sakineh Shab-bidar ) 한국임상영양학회 2021 Clinical Nutrition Research Vol.10 No.2

There is a growing body of evidence linking vitamin D and its antiadipogenic activity with body composition. The aim of this study was to investigate the association between vitamin D levels, visceral adiposity index (VAI), and lipid accumulation product index among a group of Iranian people. This cross-sectional study was conducted with 270 Iranian adults. Body composition was measured via bio-impedance analysis. The 25-hydroxyvitamin D [25(OH)D] was also measured using the enzyme-linked immunosorbent assay method. The VAI and lipid accumulation product index were calculated. Multiple linear and logistic regression after controlling for confounder was used to report the results. Multiple linear regression showed that serum 25(OH)D levels were positively correlated with age (crude: β ± standard error [SE] = 0.23 ± 0.06, p ≤ 0.001; model I: β ± SE = 0.18 ± 0.05, p = 0.002) and percent body fat (crude: β ± SE = 0.10 ± 0.04, p = 0.02). Binary logistic regression analysis showed a higher chance of greater percent body fat and lipid accumulation product index in the crude model (odds ratio [OR], 2.05; 95% confidence interval [CI], 1.13-3.72 for percent body fat and OR, 2.07; 95% CI, 1.14-3.76 for lipid accumulation product index), which disappeared after adjusting for covariates. Adults with higher vitamin D levels had higher scores of percent body fat and lipid accumulation product index. More longitudinal studies are needed to confirm these results.

Breast Cancer Trend in Iran from 2000 to 2009 and Prediction till 2020 using a Trend Analysis Method

Zahmatkesh, Bibihajar,Keramat, Afsaneh,Alavi, Nasrinossadat,Khosravi, Ahmad,Kousha, Ahmad,Motlagh, Ali Ghanbari,Darman, Mahboobeh,Partovipour, Elham,Chaman, Reza Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.3

Background: Breast cancer is the most common cancer in women worldwide with a rising incidence rate in most countries. Considering the increase in life expectancy and change in lifestyle of Iranian women, this study investigated the age-adjusted trend of breast cancer incidence during 2000-2009 and predicted its incidence to 2020. Materials and Methods: The 1997 and 2006 census results were used for the projection of female population by age through the cohort-component method over the studied years. Data from the Iranian cancer registration system were used to calculate the annual incidence rate of breast cancer. The age-adjusted incidence rate was then calculated using the WHO standard population distribution. The five-year-age-specific incidence rates were also obtained for each year and future incidence was determined using the trend analysis method. Annual percentage change (APC) was calculated through the joinpoint regression method. Results: The bias adjusted incidence rate of breast cancer increased from 16.7 per 100,000 women in 2000 to 33.6 per 100,000 women in 2009. The incidence of breast cancer had a growing trend in almost all age groups above 30 years over the studied years. In this period, the age groups of 45-65 years had the highest incidence. Investigation into the joinpoint curve showed that the curve had a steep slope with an APC of 23.4% before the first joinpoint, but became milder after this. From 2005 to 2009, the APC was calculated as 2.7%, through which the incidence of breast cancer in 2020 was predicted as 63.0 per 100,000 women. Conclusions: The age-adjusted incidence rate of breast cancer continues to increas in Iranian women. It is predicted that this trend will continue until 2020. Therefore, it seems necessary to prioritize the prevention, control and care for breast cancer in Iran.

Retinoic acid loaded with chitosan nanoparticles improves spermatogenesis in scrotal hyperthermia in mice

Fatemeh Mazini,Mohammad-Amin Abdollahifar,Hassan Niknejad,Asma Manzari-Tavakoli,Mohsen Zhaleh,Reza Asadi-Golshan,Ali Ghanbari The Korean Society for Reproductive Medicine 2023 Clinical and Experimental Reproductive Medicine Vol.50 No.4

Objective: High temperatures can trigger cellular oxidative stress and disrupt spermatogenesis, potentially leading to male infertility. We investigated the effects of retinoic acid (RA), chitosan nanoparticles (CHNPs), and retinoic acid loaded with chitosan nanoparticles (RACHNPs) on spermatogenesis in mice induced by scrotal hyperthermia (Hyp). Methods: Thirty mice (weighing 25 to 30 g) were divided into five experimental groups of six mice each. The groups were as follows: control, Hyp induced by a water bath (43 ℃C for 30 minutes/day for 5 weeks), Hyp+RA (2 mg/kg/day), Hyp+CHNPs (2 mg/kg/72 hours), and Hyp+RACHNPs (4 mg/kg/72 hours). The mice were treated for 35 days. After the experimental treatments, the animals were euthanized. Sperm samples were collected for analysis of sperm parameters, and blood serum was isolated for testosterone measurement. Testis samples were also collected for histopathology assessment, reactive oxygen species (ROS) evaluation, and RNA extraction, which was done to compare the expression levels of the bax, bcl2, p53, Fas, and FasL genes among groups. Additionally, immunohistochemical staining was performed. Results: Treatment with RACHNPs significantly increased stereological parameters such as testicular volume, seminiferous tubule length, and testicular cell count. Additionally, it increased testosterone concentration and improved sperm parameters. We observed significant decreases in ROS production and caspase-3 immunostaining in the RACHNP group. Moreover, the expression levels of bax, p53, Fas, and FasL significantly decreased in the groups treated with RACHNPs and RA. Conclusion: RACHNPs can be considered a potent antioxidative and antiapoptotic agent for therapeutic strategies in reproductive and regenerative medicine.