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Insilico profiling of microRNAs in Korean ginseng (Panax ginseng Meyer)
Ramya Mathiyalagan,Sathiyamoorthy Subramaniyam,Sathishkumar Natarajan,Yeon Ju Kim,Myung Suk Sun,Se Young Kim,Yu-Jin Kim,Deok Chun Yang 고려인삼학회 2013 Journal of Ginseng Research Vol.37 No.2
MicroRNAs (miRNAs) are a class of recently discovered non-coding small RNA molecules, on average approximately 21 nucleotides in length, which underlie numerous important biological roles in gene regulation in various organisms. The miRNA database (release 18) has 18,226 miRNAs, which have been deposited from different species. Although miRNAs have been identified and validated in many plant species, no studies have been reported on discovering miRNAs in Panax ginseng Meyer, which is a traditionally known medicinal plant in oriental medicine, also known as Korean ginseng. It has triterpene ginseng saponins called ginsenosides, which are responsible for its various pharmacological activities. Predicting conserved miRNAs by homologybased analysis with available expressed sequence tag (EST) sequences can be powerful, if the species lacks whole genome sequence information. In this study by using the EST based computational approach, 69 conserved miRNAs belonging to 44 miRNA families were identified in Korean ginseng. The digital gene expression patterns of predicted conserved miRNAs were analyzed by deep sequencing using small RNA sequences of flower buds, leaves, and lateral roots. We have found that many of the identified miRNAs showed tissue specific expressions. Using the insilico method, 346 potential targets were identified for the predicted 69 conserved miRNAs by searching the ginseng EST database, and the predicted targets were mainly involved in secondary metabolic processes, responses to biotic and abiotic stress, and transcription regulator activities, as well as a variety of other metabolic processes.
Ramya, E.,Muthtamilselvan, M.,Doh, Deog Hee Elsevier 2018 Applied Mathematics and Computation Vol.324 No.-
<P><B>Abstract</B></P> <P>A mathematical model is developed to examine the effects of radiation and slanted magnetic on boundary layer flow of a micropolar fluid containing gyrostatic microorganisms through a vertical fixed or continuous moving porous plate. The governing boundary layer equations are cast into a matrix form and solved analytically by utilizing the state space approach and the inversion of the Laplace transform is carried out, utilizing numerical approach. Numerical outcomes for the momentum, microrotation, density of motile microorganism and temperature distributions are given and illustrated graphically for the problem. Excellent agreement is found when present solutions are compared with the numerical solutions by utilizing the Crank–Nicolson implicit finite difference method. It is found that the density of the motile microorganisms is increasing functions of the bioconvection Lewis number in both cases moving and fixed plate.</P>
Orchid floral volatiles: Biosynthesis genes and transcriptional regulations
Ramya, Mummadireddy,An, Hye Ryun,Baek, Yun Su,Reddy, Kondreddy Eswar,Park, Pue Hee Elsevier 2018 Scientia horticulturae Vol.235 No.-
<P>Floral scent and colour are key trait for many floricultural crops. Floral volatiles are biologically and economically important plant derived compounds and they play vital role in pollinator attraction, plant defense and interaction with surrounding the environment. Orchidaceae is one of the largest and most widespread families of flowering plants, with more than 25,000 species. Orchids are one of the most significant plants in ecologically adopted every habitation on earth. Floral volatiles is not limited in its role to pollinator attraction, it's widely used for perfumes, cosmetics, flavourings and therapeutic applications. Even though biochemistry of floral volatiles is still new field for investigation. Due to importance of the plant biology orchid floral scent research were deeply needed. Analysis of the biosynthesis mechanisms involved in floral scent is necessary to understand the fine-scale molecular functions and to breed to new cultivars through floral volatile genetic engineering approaches. Now a days many researchers developed floral scent models in plants. However, little is known about the pathways responsible for floral scent in orchids, due to the long life cycle and large genome size. Only some terpenoid pathways were reported in orchids. In this review, we mainly concentrated orchid floral volatile regulation and compounds synthesis responsible pathways. Moreover,we mentioned emission of orchid floral volatiles and their function in pollination ecology. This information will provide a basic information on orchid floral scent research.</P>
Ramya R.S.,Srivastava Chitra,Subramanian Sabtharishi,Ranjith M. 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.1
Resistance developed by Tribolium castaneum (Herbst), a major pest of stored grain and grain products, to phosphine is a serious concern. Treatment of phosphine is given to all the stages (egg, larva, pupa and adult) of insects simultaneously in the infected commodity. Effective resistance management tactics need a proper un derstanding of expression of resistance in relation to dosage in immature stages including larvae. So, this study was undertaken to understand the genetic basis of phosphine resistance in larval stage of T. castaneum by crossing the resistant and susceptible parents to obtain the F 1 , F 2 and the back-cross progenies. The resistant parent had a resistance ratio of 37.75 when compared to the susceptible parent. The susceptibility levels of the reciprocal F 1 hybrids did not show any significant difference indicating autosomal mode of inheritance of resistance. Level of dominance for the reciprocal F 1 hybrids was estimated (-0.70 and − 0.68) which showed that the resistance is expressed as incompletely recessive in larval stage of T. castaneum. χ 2 analysis showed that the observed and expected mortalities differed significantly (P < 0.05) in most of the concentrations indicating that more than one gene is responsible for phosphine resistance. Two plateaus at ~ 40 % and ~ 90 % mortality levels for the backcross progeny to the resistant parent further confirmed the involvement of two or more genes in imparting resistance to phosphine. Our results will aid in enhancing the knowledge on the development of resistance to phosphine in the field and thus facilitate in designing suitable resistance management tactics.