Derivation and Culture of Putative Parthenogenetic Embryonic Stem Cells in New Gelatin Substrates Modified with Galactomannan

Rafael R. Ruggeri,Adriana Bos-Mikich,Fabiana F. Bressan,Nataly M. Siqueira,Flávio Meirelles,Nilo Frantz,Yeda F. Watanabe,Rosane M. D. Soares 한국고분자학회 2014 Macromolecular Research Vol.22 No.10

Human embryonic stem cells (ESC) lines to be used for cell therapies must be created and maintainedunder strict conditions, excluding the use of undefined supplements. Two key steps in the creation of a new embryonicstem cell line are adherence to the substrate and derivation towards the formation of a primary colony. Thebovine parthenote embryo model was used to test different matrices of gelatin nanofibers and gelatin/galactomannanfilms to be used for ESC derivation and culturing. Gelatin/galactomannan films were made in two concentrations ofgalactomannan, 0.1 and 0.3%, in an aqueous solution of gelatin and tested for gel cytotoxicity using cumulus cells(CCs). CCs showed normal cell morphology, with no sign of lysis or degeneration in any of the matrices tested. Innercell masses of parthenote blastocysts (n=116) were placed onto the gel matrices for culture. There were three or fourrepeats for each matrix. Our results showed a good rate of inner cell mass (ICM) adherence on the gelatin/galactomannanfilms (41%-44%) and one derivative of the gel nanofiber (17% adherence to the substrate). These resultsencouraged us to try new gelatin formulations to increase the rates of derivation and cell proliferation under definedculture conditions to comply with good manufacturing practice directives for the potential therapeutic use of ESCs.

Oral Administration of Aloe vera (L.) Burm. f. (Xanthorrhoeaceae) and Honey Improves the Host Body Composition and Modulates Proteolysis Through Reduction of Tumor Progression and Oxidative Stress in Rats

Rebeka Tomasin,Rafael Siqueira de Andrade,Maria Cristina Cintra Gomes-Marcondes 한국식품영양과학회 2015 Journal of medicinal food Vol.18 No.10

Oxidative stress has a dual role in cancer; it is linkedwith tumorigenic events and hostwasting, aswell as senescence and apoptosis. Researchers have demonstrated the importance of coadjuvant therapies in cancer treatment, and Aloe vera and honey have immunomodulatory, anticancer, and antioxidant properties. The preventive and therapeutic effects of Aloe vera (L.) Burm. f. (Xanthorrhoeaceae) and honey in tumor progression and host wasting were analyzed in Walker 256 carcinoma-bearing rats. The animalswere distributed into the following groups:C= control-untreated,W= tumor-untreated,WA= treated after tumor induction, A= control-treated, AW= treated before tumor induction, and AWA= treated before and after tumor induction. Proteolysis and oxidative stress were analyzed in the tumor, liver, muscle, and myocardial tissues. The results suggest that the Aloe vera and honey treatment affect the tumor and host by different mechanisms; the treatment-modulated host wasting and cachexia, whereas it promoted oxidative stress and damage in tumor tissues, particularly in a therapeutic context (WA).

Effects of photobiomodulation on different application points and different phases of complex regional pain syndrome type I in the experimental model

( Jaquelini Betta Canever ),( Rafael Inácio Barbosa ),( Ketlyn Germann Hendler ),( Lais Mara Siqueira Das Neves ),( Heloyse Uliam Kuriki ),( Aderbal Silva Aguiar Júnior ),( Marisa De Cassia Registro F 대한통증학회 2021 The Korean Journal of Pain Vol.34 No.3

Background: Complex regional pain syndrome type I (CRPS-I) consists of disorders caused by spontaneous pain or induced by some stimulus. The objective was to verify the effects of photobiomodulation (PBM) using 830 nm wavelength light at the affected paw and involved spinal cord segments during the warm or acute phase. Methods: Fifty-six mice were randomized into seven groups. Group (G) 1 was the placebo group; G2 and G3 were treated with PBM on the paw in the warm and acute phase, respectively; G4 and G5 treated with PBM on involved spinal cord segments in the warm and acute phase, respectively; G6 and G7 treated with PBM on paw and involved spinal cord segments in the warm and acute phase, respectively. Edema degree, thermal and mechanical hyperalgesia, skin temperature, and functional quality of gait (Sciatic Static Index [SSI] and Sciatic Functional Index [SFI]) were evaluated. Results: Edema was lower in G3 and G7, and these were the only groups to return to baseline values at the end of treatment. For thermal hyperalgesia only G3 and G5 returned to baseline values. Regarding mechanical hyperalgesia, the groups did not show significant differences. Thermography showed increased temperature in all groups on the seventh day. In SSI and SFI assessment, G3 and G7 showed lower values when compared to G1, respectively. Conclusions: PBM irradiation in the acute phase and in the affected paw showed better results in reducing edema, thermal and mechanical hyperalgesia, and in improving gait quality, demonstrating efficacy in treatment of CRPS-I symptoms.

Treatment efficacy of gingival recession defects associated with non-carious cervical lesions: a systematic review

Lívia Maria Lopes de Oliveira,Camila Agra Souza,Sinara Cunha,Rafael Siqueira,Bruna de Carvalho Farias Vajgel,Renata Cimões 대한치주과학회 2022 Journal of Periodontal & Implant Science Vol.52 No.2

Purpose: This systematic review aimed to compare the efficacy, defined in terms of the mean percentage of root coverage (mRC), of surgical treatment approaches combined with adhesive restorations of non-carious cervical lesions (NCCLs) to that of root coverage alone in patients with a single gingival recession (GR) and NCCL. Methods: A literature search was conducted to identify longitudinal studies reporting the mRC following treatment for the correction of GR defects associated with NCCLs using a combination of surgical and restorative techniques in systemically and periodontally healthy patients. Results: The search resulted in the retrieval of 12,409 records. Seven publications met the inclusion criteria for the qualitative synthesis of data. The mRCs ranged from 69% to 97%. In the medium term, the gingival margin position was more stable when a connective tissue graft (CTG) was used, independently of whether restoration of teeth with NCCLs was performed. Conclusions: The strength of the evidence was limited by methodological heterogeneity in terms of study design as well as the unit and period of analysis, which precluded a metaanalysis. Although no definitive conclusion could be drawn due to the lack of sufficient evidence to estimate the effectiveness of the interventions, CTG-based procedures contributed to gingival margin stability regardless of the performance of restoration to treat NCCLs.

Treatment efficacy of gingival recession defects associated with non-carious cervical lesions: a systematic review

Oliveira, Livia Maria Lopes de,Souza, Camila Agra,Cunha, Sinara,Siqueira, Rafael,Vajgel, Bruna de Carvalho Farias,Cimoes, Renata Korean Academy of Periodontology 2021 Journal of Periodontal & Implant Science Vol.51 No.-

Purpose: This systematic review aimed to compare the efficacy, defined in terms of the mean percentage of root coverage (mRC), of surgical treatment approaches combined with adhesive restorations of non-carious cervical lesions (NCCLs) to that of root coverage alone in patients with a single gingival recession (GR) and NCCL. Methods: A literature search was conducted to identify longitudinal studies reporting the mRC following treatment for the correction of GR defects associated with NCCLs using a combination of surgical and restorative techniques in systemically and periodontally healthy patients. Results: The search resulted in the retrieval of 12,409 records. Seven publications met the inclusion criteria for the qualitative synthesis of data. The mRCs ranged from 69% to 97%. In the medium term, the gingival margin position was more stable when a connective tissue graft (CTG) was used, independently of whether restoration of teeth with NCCLs was performed. Conclusions: The strength of the evidence was limited by methodological heterogeneity in terms of study design as well as the unit and period of analysis, which precluded a metaanalysis. Although no definitive conclusion could be drawn due to the lack of sufficient evidence to estimate the effectiveness of the interventions, CTG-based procedures contributed to gingival margin stability regardless of the performance of restoration to treat NCCLs.