True Digestibility of Phosphorus in Different Resources of Feed Ingredients in Growing Pigs

Wu, X.,Ruan, Z.,Zhang, Y.G.,Hou, Y.Q.,Yin, Y.L.,Li, T.J.,Huang, R.L.,Chu, W.Y.,Kong, X.F.,Gao, B.,Chen, L.X. Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.1

To determine the true digestible phosphorus (TDP) requirement of growing pigs, two experiments were designed with the experimental diets containing five true digestible P levels (0.16%, 0.20%, 0.23%, 0.26% and 0.39%) and the ratio of total calcium to true digestible P (TDP) kept at 2:1. In Experiment 1, five barrows (Duroc${\times}$Landrace${\times}$Yorkshire) with an average initial body weight of 27.9 kg were used in a $5{\times}5$ Latin-square design to evaluate the effect of different dietary P levels on the digestibility and output of P and nitrogen. In Experiment 2, sixty healthy growing pigs (Duroc${\times}$Landrace${\times}$Yorkshire) with an average body weight (BW) of 21.4 kg were assigned randomly to one of the five dietary treatments (12 pigs/diet), and were used to determine the true digestible phosphorus (TDP) requirement of growing pigs on the basis of growth performance and serum biochemical indices. The results indicated that the true digestibility of P increased (p<0.05) linearly with increasing dietary TDP level below 0.26%. The true P digestibility was highest (56.6%) when dietary TDP was 0.34%. Expressed as g/kg dry matter intake (DMI), fecal P output increased (p<0.05) linearly with increasing P input. On the basis of g/kg fecal dry matter (DM), fecal P output was lowest for Diet 4 and highest (p<0.05) for Diet 5. The apparent digestibility of crude protein (CP) did not differ (p>0.05) among the five diets, with the average nitrogen output of 12.14 g/d and nitrogen retention of 66% to 74% (p>0.05), which suggested that there was no interaction between dietary P and CP protein levels. During the 28-d experimental period of Experiment 2, the average daily gain (ADG) of pigs was affected by dietary TDP levels as described by Eq. (1): $y=-809,532x^4+788,079x^3-276,250x^2+42,114x-1,759$; ($R^2=0.99$; p<0.01; y = ADG, g/d; x = dietary TDP, %), F/G for pigs by Eq. (2): $y=3,651.1x^4-3,480.4x^3+1,183.8x^2-172.5x+10.9$ ($R^2=0.99$; p<0.01; y = F/G; x = dietary TDP, %), and Total P concentrations in serum by Eq. (3): $y=-3,311.7x^4+3,342.7x^3-1,224.6x^2+195.6x-8.7$ (R2 = 0.99; p<0.01; y = total serum P concentration and x = dietary TDP, %). The highest ADG (782 g/d), the lowest F/G (1.07) and the highest total serum P concentration (3.1 mmol/L) were obtained when dietary TDP level was 0.34%. Collectively, these results indicate that the optimal TDP requirement of growing pigs is 0.34% of the diet at a total Ca to TDP ratio of 2:1.

Effect of Triticale Dried Distillers Grains with Solubles on Ruminal Bacterial Populations as Revealed by Real Time Polymerase Chain Reaction

Wu, R.B.,Munns, K.,Li, J.Q.,John, S.J.,Wierenga, K.,Sharma, R.,Mcallister, T.A. Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.11

Real time PCR was used in this study to determine the effect of triticale dried distillers grains with solubles (TDDGS) as a replacement for grain or barley silage in finishing diets on the presence of six classical ruminal bacterial species (Succinivibrio dextrinosolvens, Selenomonas ruminantium, Streptococcus bovis, Megasphaera elsdenii, Prevotella ruminicola and Fibrobacter succinogenes) within the rumen contents of feedlot cattle. This study was divided into a step-wise adaptation experiment (112 days) that examined the effects of adaptation to diets containing increasing levels of TDDGS up to 30% (n = 4), a short-term experiment comparing animals (n = 16) fed control, 20%, 25% or 30% TDDGS diets over 28 days, and a rapid transition experiment (56 days) where animals (n = 4) were rapidly switched from a diet containing 30% TDDGS to a barley-based diet with no TDDGS. It was found that feeding TDDGS as replacement for barley grain (control vs. 20% TDDGS) decreased 16S rRNA copy numbers of starch-fermenting S. ruminantium and S. bovis (p<0.001 and p = 0.04, respectively), but did not alter 16S rRNA copy numbers of the other rumen bacteria. Furthermore, feeding TDDGS as a replacement barley silage (20% vs. 25% and 30% TDDGS) increased 16S rRNA copy numbers of S. ruminantium, M. elsdenii and F. succinogenes (p<0.001; p = 0.03 and p<0.001, respectively), but decreased (p<0.001) the 16S rRNA copy number of P. ruminicola. Upon removal of 30% TDDGS and return to the control diet, 16S rRNA copy numbers of S. ruminantium, M. elsdenii and F. succinogenes decreased (p = 0.01; p = 0.03 and p = 0.01, respectively), but S. dextrinosolvens and S. bovis increased (p = 0.04 and p = 0.009, respectively). The results suggest that replacement of TDDGS for grain reduces 16S rRNA copy numbers of starch-fermenting bacteria, whereas substitution for barley silage increases 16S rRNA copy numbers of bacteria involved in fibre digestion and the metabolism of lactic acid. This outcome supports the contention that the fibre in TDDGS is highly fermentable.

Numerical study on catalytic combustion of methane with ozone using Pd-exchanged zeolite X

Wu, R.,Chen, R.,Wang, H.,Wang, Z.,Zhu, X.,Liao, Q.,Hui, K.,Hui, K. Springer Science and Business Media 2015 Science China. Chemistry Vol.58 No.5

Short-circuiting in fullerene devices studied by in situ electrical measurement in high vacuum and infrared imaging analysis?

H.R. Wu,M.L. Wang,Q.L. Song,Y. Wu,Z.T. Xie,X.D. Gao,X.M. Ding,X.Y. Hou 한국물리학회 2007 Current Applied Physics Vol.7 No.3

In the present work, currentvoltage (IV) characteristics of fullerene devices (ITOnC60nAl) are reexamined byin situelectrical mea-surement in high vacuum and by infrared imaging analysis. Two kinds ofIV curves are detected: ‘ohmic’ and nonohmic. Degradationprocesses of the two dierent devices are measured, and ‘ohmic’ degradation processes are ascribed to short-circuiting. ITOnC60nAldevices in high vacuum are conrmed to be intrinsically nonohmic. Surface temperature distribution of the two dierent devices is mea-buers are inserted between fullerene layer and cathode and this is found to be eective.

Cluster Analysis of 12 Chinese Native Chicken Populations Using Microsatellite Markers

Chen, G.H.,Wu, X.S.,Wang, D.Q.,Qin, J.,Wu, S.L.,Zhou, Q.L.,Xie, F.,Cheng, R.,Xu, Q.,Liu, B.,Zhang, X.Y.,Olowofeso, O. Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.8

The genomes of Chinese native chicken populations were screened using microsatellites as molecular markers. A total of, 528 individuals comprisede12 Chinese native chicken populations were typed for 7 microsatellite markers covering 5 linkage groups and genetic variations and genetic distances were also determined. In the 7 microsatellite loci, the number of alleles ranged from 2 to 7 per locus and the mean number of alleles was 4.6 per locus. By using fuzzy cluster, 12 Chinese native chicken populations were divided into three clusters. The first cluster comprised Taihe Silkies, Henan Game Chicken, Langshan Chicken, Dagu Chicken, Xiaoshan Chicken, Beijing Fatty Chicken and Luyuan Chicken. The second cluster included Chahua Chicken, Tibetan Chicken, Xianju Chicken and Baier Chicken. Gushi Chicken formed a separate cluster and demonstrated a long distance when comparing with other chicken populations.

Bioreactor application on adventitious root culture of Astragalus membranaceus

Wu, S. Q.,Lian, M. L.,Gao, R.,Park, S. Y.,Piao, X. C. Springer Science + Business Media 2011 In vitro cellular & developmental biology. journal Vol.47 No.6

Marker-Assisted Mating Applied in In-Situ Conservation of Indigenous Animals in Small Populations : (1) Choosing Mating Schemes for Maximum Heterozygosity

Wu, X.L.,Liu, R.Z.,Shi, Q.S.,Liu, X.C.,Li, X.,Wu, M.S. Asian Australasian Association of Animal Productio 2000 Animal Bioscience Vol.13 No.4

Maintaining maximum genetic variability is of critical importance with in-situ conservation of animal species in small populations. Marker-assisted mating (MAM) was suggested to achieve maximum heterozygosity in offspring populations. The aims of this research was to investigate and decide the effectiveness and promising types of MAM to achieve this goal. Analysis of variance with simulation data revealed that the heterozygosity in offspring populations was significantly determined by sire heterozygosity from mating of non-inbred parent animals, and significantly by sire heterozygosity and percent parental difference in offspring reproduced by inbred parents. Seven types of marker-assisted mating schemes were examined, in which offspring exhibited heterozygosity that was -0.01 to 7.37% below or above that from random mating of non-inbred parent animals, and 0.00 to 16.39% above that from random mating of inbred parent animals. The great increase in offspring heterozygosity was observed with mating by tandem maximizing sire heterozygosity, percent parental difference, and dam heterozygosity. Random mating resulted in fluctuation of offspring heterozygosity. These results suggested that MAM was a promising method for maintaining maximum offspring variability in in-situ conservation of animal species in small populations.

Wind-induced lateral-torsional coupled responses of tall buildings

Wu, J.R.,Li, Q.S.,Tuan, Alex Y. Techno-Press 2008 Wind and Structures, An International Journal (WAS Vol.11 No.2

Based on the empirical formulas for power spectra of generalized modal forces and local fluctuating wind forces in across-wind and torsional directions, the wind-induced lateral-torsional coupled response analysis of a representative rectangular tall building was conducted by setting various parameters such as eccentricities in centers of mass and/or rigidity and considering different torsional to lateral stiffness ratios. The eccentricity effects on the lateral-torsional coupled responses of the tall building were studied comprehensively by structural dynamic analysis. Extensive computational results indicated that the torsional responses at the geometric center of the building may be significantly affected by the eccentricities in the centers of mass and/or rigidity. Covariance responses were found to be in the same order of magnitude as the along-wind or across-wind responses in many eccentricity cases, suggesting that the lateral-torsional coupled effects on the overall wind-induced responses can not be neglected for such situations. The calculated results also demonstrated that the torsional motion contributed significantly to the total responses of rectangular tall buildings with mass and/or rigidity eccentricities. It was shown through this study that the framework presented in this paper provides a useful tool to evaluate the wind-induced lateral-torsional coupled responses of rectangular buildings, which will enable structural engineers in the preliminary design stages to assess the serviceability of tall buildings, potential structural vibration problems and the need for a detailed wind tunnel test.

Wind-induced lateral-torsional coupled responses of tall buildings

J. R. Wu,Q. S. Li,Alex Y. Tuan 한국풍공학회 2008 Wind and Structures, An International Journal (WAS Vol.11 No.2

Based on the empirical formulas for power spectra of generalized modal forces and local fluctuating wind forces in across-wind and torsional directions, the wind-induced lateral-torsional coupled response analysis of a representative rectangular tall building was conducted by setting various parameters such as eccentricities in centers of mass and/or rigidity and considering different torsional to lateral stiffness ratios. The eccentricity effects on the lateral-torsional coupled responses of the tall building were studied comprehensively by structural dynamic analysis. Extensive computational results indicated that the torsional responses at the geometric center of the building may be significantly affected by the eccentricities in the centers of mass and/or rigidity. Covariance responses were found to be in the same order of magnitude as the along-wind or across-wind responses in many eccentricity cases, suggesting that the lateral-torsional coupled effects on the overall wind-induced responses can not be neglected for such situations. The calculated results also demonstrated that the torsional motion contributed significantly to the total responses of rectangular tall buildings with mass and/or rigidity eccentricities. It was shown through this study that the framework presented in this paper provides a useful tool to evaluate the wind-induced lateral-torsional coupled responses of rectangular buildings, which will enable structural engineers in the preliminary design stages to assess the serviceability of tall buildings, potential structural vibration problems and the need for a detailed wind tunnel test.

Activation of antiferromagnetic domain switching in exchange-coupled Fe/CoO/MgO(001) systems

Li, Q.,Chen, G.,Ma, T. P.,Zhu, J.,N'Diaye, A. T.,Sun, L.,Gu, T.,Huo, Y.,Liang, J. H.,Li, R. W.,Won, C.,Ding, H. F.,Qiu, Z. Q.,Wu, Y. Z. American Physical Society 2015 Physical review. B, Condensed matter and materials Vol.91 No.13