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      • SCIESCOPUSKCI등재

        Performance of Broiler Chickens Fed Low Protein, Limiting Amino Acid Supplemented Diets Formulated Either on Total or Standardized Ileal Digestible Amino Acid Basis

        Kumar, C. Basavanta,Gloridoss, R.G.,Singh, K.C.,Prabhu, T.M.,Suresh, B.N. Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.11

        The aim of present experiment was to investigate the effect of protein reduction in commercial broiler chicken rations with incorporation of de-oiled rice bran (DORB) and supplementation of limiting amino acids (valine, isoleucine, and/or tryptophan) with ration formulation either on total amino acid (TAA) or standardized ileal digestible amino acids (SIDAA). The experimental design consisted of $T_1$, TAA control; $T_2$ and $T_3$, 0.75% and 1.5% protein reduction by 3% and 6% DORB incorporation, respectively by replacing soybean meal with supplemental limiting amino acids to meet TAA requirement; $T_4$, SIDAA control, $T_5$ and $T_6$, 0.75% and 1.5% protein reduction by DORB incorporation (3% and 6%) with supplemental limiting amino acids on SIDAA basis. A total of 360 dold fast growing broiler chicks (Vencobb-400) were divided into 36 homogenous groups of ten chicks each, and six dietary treatments described were allocated randomly with six replications. During 42 days trial, the feed intake was significantly (p<0.05) reduced by TAA factor compared to SIDAA factor and protein factor significantly (p<0.05) reduced the feed intake at 1.5% reduction compared to normal protein group. This was observed only during pre-starter phase but not thereafter. The cumulative body weight gain (BWG) was significantly (p<0.05) reduced in TAA formulations with protein step-down of 1.5% ($T_3$, 1,993 g) compared to control ($T_1$, 2,067 g), while under SIDAA formulations, BWG was not affected with protein reduction of 1.5% ($T_6$, 2,076 g) compared to $T_4$ (2,129 g). The feed conversion ratio (FCR) was significantly (p<0.05) reduced in both TAA and SIDAA formulations with 1.5% protein step-down ($T_3$, 1.741; $T_6$, 1.704) compared to respective controls ($T_1$, 1.696; $T_4$, 1.663). The SIDAA formulation revealed significantly (p<0.05) higher BWG (2,095 g) and better FCR (1.684) compared to TAA formulation (2,028 g; 1.721). Intake of crude protein and all limiting amino acids (SID basis) was higher in SIDAA group than TAA group with resultant higher nitrogen retention (4.438 vs 4.027 g/bird/d). The nitrogen excretion was minimized with 1.5% protein reduction (1.608 g/bird) compared to normal protein group (1.794 g/bird). The serum uric acid concentration was significantly reduced in $T_3$ (9.45 mg/dL) as compared to $T_4$ (10.75 mg/dL). All carcass parameters were significantly (p<0.05) higher in SIDAA formulation over TAA formulation and 1.5% protein reduction significantly reduced carcass, breast and thigh yields. In conclusion, the dietary protein can be reduced by 0.75% with TAA formulation and 1.5% with SIDAA formulation through DORB incorporation and supplementation of limiting amino acids and among formulations, SIDAA formulation was better than TAA formulation.

      • SCIESCOPUSKCI등재

        Breakthrough behaviour of activated charcoal cloth samples against oxygen analogue of sulphur mustard

        G. K. Prasad,J. Praveen Kumar,P. V. R. K. Ramacharyulu,Beer Singh 한국탄소학회 2015 Carbon Letters Vol.16 No.1

        The breakthrough behaviour of activated charcoal cloth samples against an oxygen analogue (OA) of sulphur mustard has been studied using the modified Wheeler equation. Activated charcoal cloth samples having different surface area values in the range of 481 to 1290 m2/g were used for this purpose. Breakthrough behaviour was found to depend on the properties of the activated charcoal cloth, properties of the OA and the adsorption conditions. Activated charcoal cloth with a high surface area of 1290 m2/g, relatively large surface density of 160 g/m2 and coarser fiber structure exhibited better kinetic saturation capacity value, 0.19 g/g, against OA vapours when compared to others, thus confirming its potential use in foldable masks for protection against chemical warfare agents.

      • SCIESCOPUSKCI등재

        Accuracy of Ultrasonography in Early Pregnancy Diagnosis in Doe

        Singh, N.S.,Gawande, P.G.,Mishra, O.P.,Nema, R.K.,Mishra, U.K.,Singh, Mohan Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.6

        The present study was undertaken to evaluate the accuracy of ultrasonography in early pregnancy diagnosis in goats. Ultrasonographic scanning with real time B-mode ultrasound machine having 5 MHz linear array transducer was performed on gravid uterus (n=24) obtained from slaughterhouse (Group I). Crown rump length (CRL) measured by ultrasound was found significantly different (p<0.05) with actual CRL measured after dissection in early pregnancy. However, age predicted by ultrasound through the measurement of CRL was found highly correlated (r=0.92) with age measured after dissection through CRL and the weight of fetus. Ages predicted by ultrasound through the measurement of trunk diameter (TD) and uterine diameter (UD) and ages measured after dissection were found highly and equally correlated (r=0.98) and did not differ significantly. Data from six does synchronized (Group II) with PGF2$\alpha$ (Estrumate) at 11 days apart were collected through ultrasound from 17 to 42 days post breding. The correlation between CRL and gestational age was high (r=0.97) in day 30 to 42 post breeding. A high coefficient of correlation (r=0.98) was also observed between predicated age by ultrasound and actual age calculated after kidding. The correlation between CRL and gestational age by the formula Y=(a+bX) i.e. Y=24.42+0.39 X where Y=gestational age and X=CRL, was recorded very high (r=0.99). Accuracy of ultrasonography was lowest on day 17 to 19 (66%) and reached 100% on day 34. Data from 30 does (group III) randomly subjected to only one time ultrasounds scanning to assess the accuracy of pregnancy diagnosis were also obtained. Ages predicted by TD and UD measurements were observed to be non-significantly different with actual age obtained after kidding and correlation between ages predicted by TD and UD measurement with actual age after kidding was found equally and highly correlated (r=0.98). The operator's accuracy in the whole experiment including all three groups was found to be 92%. The sensitivity was 93% and specificity was 86%. From the present study, it was observed that CRL was the most reliable parameter to find out gestational age in early pregnancy and the new formula derived was found very accurate to find out gestational age. TD and UD were also found to be equally reliable parameter to find out gestational age in mid and late stage of pregnancy through ultrasonography. It was concluded that ultrasonography by real time B mode with 5 MHz transrectal transducer was found to be reliable, safe and accurate and practicable means in diagnosing early pregnancy diagnosis as early as 25 days post breeding.

      • SCIESCOPUSKCI등재

        Quality and Fertility of Post Thaw Sephadex Filtered Bull Semen

        Kumar, A.,Singh, J.,Nanda, A.S.,Pangaonkar, G.R. Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.6

        The present investigation was carried out to assess the effect of Sephadex (G-15) filtration on the post thaw bull semen quality and conception rate. Post thaw unfiltered (control) and Sephadex filtered semen from four healthy bulls (three cross bred and one pure bred Holstein Friesian) were subjected to microscopic examination viz. sperm concentration, individual motility, live sperm count and sperm morphology. Sixty-two healthy, normal cycling crossbred cows were inseminated with post thaw unfiltered (n=32) and filtered semen (n=30). Sephadex filtration of post thaw semen significantly (p<0.05) decreased total sperm concentration and sperm with abnormal head, mid piece and tail. The overall average total sperm concentration, head and tail defects in filtered semen decreased significantly (53.4, 1.2 and 6.4 million) than in the unfiltered semen (80.4, 2.4 and 15.7 million, respectively). However, after filtration significant (p<0.05) increase in overall average motile and live sperm concentration were observed (38.8 and 38.0) as compared to unfiltered semen (29.2 and 32.0 million, respectively). The overall conception rate recorded was 21.9% with post thaw unfiltered semen and 56.7% with filtered semen. It was concluded that Sephadex filtration of post thaw semen improved its quality and conception rate.

      • SCISCIESCOPUS

        Eu2+ and Mn2+ Co-doped BaMgAl10O17 Blue- and Green-Emitting Phosphor: A Luminescence and EPR Study

        Singh, V.,Sivaramaiah, G.,Rao, J. L.,Singh, N.,Srivastava, A. K.,Jirimali, H. D.,Li, J.,Gao, H.,Kumaran, R. S.,Singh, P. K. Springer Science + Business Media 2016 Journal of electronic materials Vol.45 No.6

        <P>Eu2+ and Mn2+ co-doped BaMgAl10O17 phosphor has been prepared by a solution combustion method. The structural, morphological and compositional analysis of the BaMgAl10O17:Eu2+ and Mn2+ powders have been studied by x-ray diffraction, scanning electron microscopy and energy dispersive x-ray spectroscopy. The electron paramagnetic resonance (EPR) spectrum exhibited resonance signals with the effective g values of g a parts per thousand 4.88 and g a parts per thousand 1.98. The resonance signal with the effective g value of g a parts per thousand 4.88 is characteristic of Eu2+ ions whereas g a parts per thousand 1.98 is due to Mn2+ ions. The number of spins participating in resonance, Gibbs free energy, magnetic susceptibility, Curie constant, effective magnetic moment, zero-field splitting parameter and hyperfine splitting constant have been evaluated. From optical and EPR correlation, it is inferred that Eu2+ and Mn2+ are present in the BaMgAl10O17 matrix.</P>

      • KCI등재

        Growth kinetics of ZnO nanocrystallites: Structural, optical and photoluminescence properties tuned by thermal annealing

        R.G. Singh,Fouran Singh,Vinod Kumar,R.M. Mehra 한국물리학회 2011 Current Applied Physics Vol.11 No.3

        In this paper, we report temperature dependent studies of ZnO nanocrystallites deposited by the sol-gel spin coating process. The films are annealed at different temperature from 500 to 900 ℃ to grow the size of crystallites in controlled environment. The crystallite size, lattice parameters, and strain in the nanocrystallites are calculated. A coarsening kinetics is understood by Lifshitz, Slyozov and Wagner (LSW) theory and the activation energy of the coarsening process is estimated. Optical absorption and Photoluminescence (PL) measurements were carried out to investigate the effect of coarsening on optical properties of the ZnO thin films. The origin of PL spectra is explained in terms of the stabilization of structural defects and size of the crystallites.

      • SCIESCOPUSKCI등재

        Molecular Characterization and Expression of LDHA and LDHB mRNA in Testes of Japanese Quail (Coturnix japonica)

        Singh, R.P.,Sastry, K.V.H.,Pandey, N.K.,Shit, N.G.,Agarwal, R.,Singh, R.,Sharma, S.K.,Saxena, V.K.,Jagmohan, Jagmohan Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.8

        The LDH isozymes are key catalysts in the glycolytic pathway of energy metabolism. It is well known that the distribution of the LDH isozymes vary in accordance with the metabolic requirements of different tissues. The substrates required for energy production change noticeably at successive stages of testes development suggesting a significant flexibility in the expression of glycolytic enzymes. Therefore, expression of LHDA and LDHB mRNAs was examined in adult and prepubertal quail testis. The mRNA of both LDHA and LDHB were expressed and no significant difference was observed in prepubertal testes. The mRNA levels of LDHB significantly increased during testicular development. In the adult testis, LDHA mRNA was not expressed. Expression studies revealed the presence of different LDH isozymes during testicular development. In contrast, electrophoresis of both testicular samples revealed only single band at a position indicative of an extreme type of LDH isozyme in quail testes. Furthermore, nucleotide and amino acid sequence analysis revealed significant similarity to chicken, duck and rock pigeon. These sequence results confirmed the similarity of LDHA and LDHB subunit protein in different avian species.

      • Gadolinium activated ZrO<sub>2</sub> ultraviolet B emitting powder phosphor - A luminescence and ESR investigation

        Singh, N.,Sivaramaiah, G.,Rao, J.L.,Watanabe, S.,Rao, T.K. Gundu,Patel, R.V.,Singh, Vijay Elsevier 2017 Journal of luminescence Vol.188 No.-

        <P><B>Abstract</B></P> <P>Gadolinium activated UV-emitting ZrO<SUB>2</SUB> phosphor has been prepared by combustion synthesis. The prepared phosphor was characterized using X-ray diffraction, scanning electron microscopy and energy dispersive X-ray techniques. The optical absorption spectrum in the 200−500nm region and excitation spectrum corresponding to the emission at 314nm for ZrO<SUB>2</SUB>:Gd<SUP>3+</SUP> phosphor has been recorded. Gd<SUP>3+</SUP> doped ZrO<SUB>2</SUB> displays electron spin resonance signals with effective g values at <I>g</I>≈1.9, 2.4 and 4.1. Low field signals are attributed to Gd<SUP>3+</SUP> ion located in distorted surroundings experiencing a relatively strong crystal field due to the proximity of charge compensator oxygen vacancies.</P>

      • SCISCIESCOPUS

        Development of Nanoparticle-Based High Current Density Cathode for THz Devices Application

        Barik, R. K.,Singh, A. K.,Shukla, S.,Singh, T. P.,Raju, R. S.,Park, G.-S Institute of Electrical and Electronics Engineers 2016 IEEE transactions on electron devices Vol.63 No.4

        <P>Scandia (Sc2O3)-doped tungsten nanoparticle-based high current density cathode is developed for the application in terahertz (THz) devices. This paper involves synthesis of scandia-doped tungsten nanoparticle powder using chemical technique, estimation of optimum porosity, and development of pellet with required porosity by optimizing process parameters. The cathode, made out of the above pellet, is tested in an analytical system containing Auger electron spectroscope and anode. The results show that the cathode can deliver more than 100 A/cm(2) current density with stable emission-strongly recommending it as a candidate for the use in a THz device. Theoretical estimates show that the void porosity is to be kept at 21% for proper surface coverage to produce good emission with long life.</P>

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